##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549725_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	792969
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21520891737256	33.0	31.0	34.0	30.0	34.0
2	32.41000215645252	34.0	31.0	34.0	30.0	34.0
3	32.46691106461917	34.0	31.0	34.0	30.0	34.0
4	35.960536918845506	37.0	35.0	37.0	35.0	37.0
5	35.92669574724863	37.0	35.0	37.0	35.0	37.0
6	35.57914243810288	37.0	35.0	37.0	33.0	37.0
7	35.91973456717728	37.0	35.0	37.0	35.0	37.0
8	36.01739664476165	37.0	35.0	37.0	35.0	37.0
9	37.79626567999506	39.0	38.0	39.0	35.0	39.0
10	37.27004964885134	39.0	37.0	39.0	34.0	39.0
11	37.168072144056076	39.0	37.0	39.0	34.0	39.0
12	37.04473188737517	39.0	37.0	39.0	33.0	39.0
13	36.97781628285595	39.0	37.0	39.0	33.0	39.0
14	38.1526705331482	40.0	37.0	41.0	33.0	41.0
15	38.210916189661894	40.0	37.0	41.0	33.0	41.0
16	38.245946562854286	40.0	37.0	41.0	34.0	41.0
17	38.20050720772186	40.0	37.0	41.0	34.0	41.0
18	38.16246915074864	40.0	37.0	41.0	33.0	41.0
19	38.198111149363974	40.0	37.0	41.0	34.0	41.0
20	38.12953722024442	40.0	37.0	41.0	34.0	41.0
21	37.98777505804136	40.0	37.0	41.0	33.0	41.0
22	38.05175359944714	40.0	37.0	41.0	34.0	41.0
23	38.01602710824761	40.0	37.0	41.0	34.0	41.0
24	37.980286745131274	40.0	37.0	41.0	33.0	41.0
25	37.91973204501058	40.0	37.0	41.0	33.0	41.0
26	37.86283448659405	40.0	36.0	41.0	33.0	41.0
27	37.803636712153946	40.0	36.0	41.0	33.0	41.0
28	37.69700454872763	40.0	36.0	41.0	33.0	41.0
29	37.631955347560876	40.0	36.0	41.0	33.0	41.0
30	37.62361706447541	40.0	36.0	41.0	33.0	41.0
31	37.57302240062348	40.0	36.0	41.0	33.0	41.0
32	37.46855173405265	40.0	36.0	41.0	33.0	41.0
33	37.429460672485305	40.0	36.0	41.0	33.0	41.0
34	37.340068779485705	40.0	36.0	41.0	32.0	41.0
35	37.290390923226504	40.0	36.0	41.0	32.0	41.0
36	37.13896003500767	39.0	35.0	41.0	31.0	41.0
37	37.09750318108274	39.0	35.0	41.0	31.0	41.0
38	37.02399715499597	39.0	35.0	41.0	31.0	41.0
39	36.9713267479561	39.0	35.0	41.0	31.0	41.0
40	36.8669985838034	39.0	35.0	41.0	31.0	41.0
41	36.81610125994837	39.0	35.0	41.0	31.0	41.0
42	36.784200895621396	39.0	35.0	41.0	31.0	41.0
43	36.730796538073996	39.0	35.0	41.0	31.0	41.0
44	36.57458362180615	39.0	35.0	41.0	30.0	41.0
45	36.605504124373084	39.0	35.0	41.0	31.0	41.0
46	36.524793529129134	39.0	35.0	40.0	30.0	41.0
47	36.35326979995435	39.0	35.0	40.0	30.0	41.0
48	36.287088145942654	39.0	35.0	40.0	30.0	41.0
49	36.28753078619719	39.0	35.0	40.0	30.0	41.0
50	36.196693691682775	38.0	35.0	40.0	30.0	41.0
51	35.30228420026508	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	5.0
10	19.0
11	17.0
12	11.0
13	13.0
14	9.0
15	20.0
16	20.0
17	68.0
18	119.0
19	214.0
20	395.0
21	622.0
22	1017.0
23	1563.0
24	2337.0
25	3567.0
26	4979.0
27	6438.0
28	7737.0
29	9460.0
30	12179.0
31	15495.0
32	20617.0
33	28494.0
34	47157.0
35	60228.0
36	65073.0
37	97036.0
38	171834.0
39	236129.0
40	93.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.021415717386176	4.480755237594408	57.35949324626814	16.138335798751275
2	19.572896292288856	4.49248331271462	54.728242844297824	21.206377550698704
3	20.98770569845732	4.496392671088025	52.918336025746285	21.59756560470838
4	19.272506239209854	4.442544412202746	51.82081518949669	24.464134159090708
5	17.604849622116376	5.899095677132397	51.579191620353384	24.916863080397846
6	21.71207197254874	6.476671849719219	53.555813657280424	18.25544252045162
7	84.16167592932385	2.354947040804874	9.11838924346349	4.3649877864077915
8	86.42002398580524	2.8156207871934464	6.716277685508514	4.048077541492795
9	80.49318447505514	5.945755760943997	8.593778571419563	4.967281192581298
10	35.54111194762973	35.633044923572044	15.664925110565484	13.160918018232742
11	24.480402134257456	25.255590067203133	31.06981483513227	19.194192963407144
12	24.323145040978904	22.605927848377426	34.22567590914652	18.845251201497156
13	22.841624325793315	23.567377791565622	34.49668272025767	19.094315162383396
14	18.873751685122624	27.491238623451864	32.52371782503477	21.11129186639074
15	17.01642813275172	26.84190680846288	35.77239463333371	20.36927042545169
16	19.86471097861329	25.48788161958412	33.45351457623186	21.193892825570735
17	20.07014145571895	25.118131982460852	30.647225805800733	24.164500756019468
18	20.894385530834118	24.905765546950764	32.39571786538944	21.804131056825675
19	22.12015854339829	25.806683489518505	31.952573177513877	20.120584789569325
20	24.31217361586645	26.605705897708486	31.1205103856519	17.96161010077317
21	24.67612226959692	26.137213434573102	30.58757656352266	18.599087732307314
22	22.50264512231878	23.842420069384808	30.944841475517958	22.710093332778456
23	20.5039541268322	26.65930193992451	30.0379964412228	22.798747492020496
24	21.37498439409359	25.185221616481858	30.754291781898157	22.685502207526397
25	20.018941471860817	26.603940381023722	30.57572238006782	22.801395767047637
26	19.009696469849388	28.75509635307307	30.243048593324584	21.992158583752957
27	19.806322819681476	27.747995192750285	30.9270602003357	21.51862178723254
28	17.92554311707015	27.00811759349987	32.79661626116532	22.26972302826466
29	19.50731995828336	25.978064716275163	31.73037029190296	22.78424503353851
30	22.05622161774294	25.409063910442903	30.53461106297976	22.00010340883439
31	22.205155560936177	26.949603326233433	27.497796256852414	23.347444855977976
32	20.79261610479098	27.645469116699388	29.50417986075118	22.057734917758452
33	21.705388230813565	26.91580629255368	28.798603728519023	22.580201748113733
34	19.24312299724201	25.469848127732607	30.835253332728012	24.451775542297366
35	19.46608253286068	25.071976331987756	31.783083575776605	23.678857559374958
36	21.99985119216514	24.81446311268158	31.277263045591948	21.90842264956133
37	20.52955411876126	27.112409186235524	29.855896006022935	22.502140688980276
38	19.343379123269635	28.733405719517407	28.320400923617445	23.602814233595513
39	21.39730556932238	26.108712950947645	29.210095224403474	23.2838862553265
40	21.83237932378189	24.6298405107892	30.096636816823864	23.441143348605053
41	18.636668016025848	24.928465047183433	31.70741857500104	24.727448361789676
42	20.52841914374963	24.401332208446988	31.7120845833822	23.35816406442118
43	20.719725487377186	24.183921439551863	31.064392176743354	24.0319608963276
44	20.215418257208036	25.80554851450687	29.304676475372933	24.674356752912157
45	21.565407979378765	24.614329185630208	28.445248174897124	25.375014660093896
46	22.445013613394725	24.638037552539885	29.571395603106804	23.345553230958586
47	18.563651290277424	25.325327976251277	32.2098341801508	23.901186553320493
48	19.706066693653852	24.213052464850453	32.159769171304305	23.921111670191394
49	20.664490036811024	22.921198684942286	32.39609619039332	24.01821508785337
50	20.721743220731202	23.809253577378183	30.10117671687039	25.367826485020224
51	18.89846891870931	23.808623035705054	28.60326191818344	28.689646127402206
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	187.0
1	526.5
2	866.0
3	2189.5
4	3513.0
5	2489.0
6	1465.0
7	1443.5
8	1422.0
9	1569.5
10	1717.0
11	1826.0
12	1935.0
13	1935.0
14	1935.0
15	1893.5
16	1852.0
17	2047.5
18	2243.0
19	2419.0
20	2595.0
21	3300.5
22	4006.0
23	4701.0
24	5396.0
25	6125.5
26	8771.5
27	10688.0
28	12061.5
29	13435.0
30	16355.5
31	19276.0
32	20733.0
33	22190.0
34	24864.0
35	27538.0
36	28921.0
37	30304.0
38	32733.5
39	35163.0
40	41181.5
41	47200.0
42	49543.0
43	51886.0
44	55771.5
45	59657.0
46	65358.5
47	71060.0
48	79119.5
49	87179.0
50	80191.0
51	73203.0
52	63737.0
53	54271.0
54	45904.5
55	37538.0
56	33861.5
57	30185.0
58	27401.5
59	24618.0
60	22327.0
61	20036.0
62	17721.0
63	15406.0
64	12955.5
65	10505.0
66	8401.0
67	6297.0
68	5058.5
69	3820.0
70	3069.5
71	2319.0
72	1932.5
73	1546.0
74	1145.0
75	547.0
76	350.0
77	280.5
78	211.0
79	217.0
80	223.0
81	166.5
82	110.0
83	64.0
84	18.0
85	10.5
86	3.0
87	2.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	792969.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.176588110254297
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.94729630173863	19.009288610182644
2	12.341361502549832	6.707921965490916
3	4.956582447565914	4.04108998836045
4	2.586876551810408	2.812099141625055
5	1.546724575511832	2.101734835434649
6	0.9861405427075534	1.607996120879149
7	0.658403201525463	1.2525206832831184
8	0.4762307989012285	1.0353862613724825
9	0.3637735090146883	0.8897510537922657
>10	4.867634623160937	33.914502116039586
>50	1.1728414041915067	20.36318302011556
>100	0.09050715353729401	4.176223357949441
>500	0.003282642874409627	0.6312317828412091
>1k	0.0023447449102925906	1.4570710626334633
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC	2820	0.35562550364516143	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGCT	2620	0.33040383671997264	TruSeq Adapter, Index 19 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC	2126	0.26810631941475643	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2005	0.2528472109250173	No Hit
GCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC	1862	0.2348137190735073	TruSeq Adapter, Index 13 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTG	980	0.12358616793342488	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2610833462594375E-4	0.0	0.0	0.3549949619720317	0.0
2	1.2610833462594375E-4	0.0	0.0	1.3042123967015105	0.0
3	1.2610833462594375E-4	0.0	0.0	1.9245392947265278	0.0
4	1.2610833462594375E-4	0.0	0.0	2.60098440166009	0.0
5	1.2610833462594375E-4	0.0	0.0	3.6969919378941674	0.0
6	1.2610833462594375E-4	0.0	0.0	4.342792719513626	0.0
7	1.2610833462594375E-4	0.0	0.0	4.977495967686	0.0
8	1.2610833462594375E-4	0.0	0.0	5.910697643917985	0.0
9	1.2610833462594375E-4	0.0	0.0	6.356995040159199	0.0
10	1.2610833462594375E-4	0.0	0.0	7.302933658188404	0.0
11	1.2610833462594375E-4	0.0	0.0	9.096824718242454	0.0
12	1.2610833462594375E-4	0.0	0.0	10.77393946043288	0.0
13	1.2610833462594375E-4	0.0	0.0	11.268158023831953	0.0
14	1.2610833462594375E-4	0.0	0.0	11.442944175623511	0.0
15	1.2610833462594375E-4	0.0	0.0	11.696926361560163	0.0
16	1.2610833462594375E-4	0.0	0.0	12.36731826843168	0.0
17	1.2610833462594375E-4	0.0	0.0	13.455002654580444	0.0
18	1.2610833462594375E-4	0.0	0.0	14.66728207533964	0.0
19	1.2610833462594375E-4	0.0	0.0	15.38862174940004	0.0
20	1.2610833462594375E-4	0.0	0.0	16.15182939055625	0.0
21	1.2610833462594375E-4	0.0	0.0	17.183647784465723	0.0
22	1.2610833462594375E-4	0.0	0.0	18.309669104340774	0.0
23	1.2610833462594375E-4	0.0	0.0	19.28890032271123	0.0
24	1.2610833462594375E-4	0.0	0.0	20.039875455408723	0.0
25	1.2610833462594375E-4	0.0	0.0	20.66575112015728	0.0
26	1.2610833462594375E-4	0.0	0.0	21.263252409615003	0.0
27	1.2610833462594375E-4	0.0	0.0	21.864032515773	0.0
28	1.2610833462594375E-4	0.0	0.0	22.49924019728388	0.0
29	1.2610833462594375E-4	0.0	0.0	23.1599217623892	0.0
30	1.2610833462594375E-4	0.0	0.0	23.87407326137592	0.0
31	1.2610833462594375E-4	0.0	0.0	24.563633635110577	0.0
32	1.2610833462594375E-4	0.0	0.0	25.21183047508793	0.0
33	1.2610833462594375E-4	0.0	0.0	25.859775098396028	0.0
34	1.2610833462594375E-4	0.0	0.0	26.45765471285763	0.0
35	1.2610833462594375E-4	0.0	0.0	27.12438947802499	0.0
36	1.2610833462594375E-4	0.0	0.0	27.739041500991842	0.0
37	1.2610833462594375E-4	0.0	0.0	28.392534891023484	0.0
38	1.2610833462594375E-4	0.0	0.0	29.02068050579531	0.0
39	1.2610833462594375E-4	0.0	0.0	29.679848770885116	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTATCG	30	2.1646465E-6	45.000004	1
CGGTAGT	30	2.1646465E-6	45.000004	12
TCGCAAC	20	7.031963E-4	45.0	16
CATATCG	20	7.031963E-4	45.0	27
GATCGAC	20	7.031963E-4	45.0	15
CAACGAA	20	7.031963E-4	45.0	17
GACGTCC	20	7.031963E-4	45.0	28
CGTTGAT	25	3.8897077E-5	45.0	25
TCGGATT	20	7.031963E-4	45.0	31
CGAATTG	20	7.031963E-4	45.0	27
CGAATAC	20	7.031963E-4	45.0	28
AGACACG	35	1.2112832E-7	45.0	10
CGTCGGT	25	3.8897077E-5	45.0	43
GACGCAT	25	3.8897077E-5	45.0	38
TGTCGCG	35	1.2112832E-7	45.0	1
ATCGCTA	25	3.8897077E-5	45.0	14
TCGGTAC	25	3.8897077E-5	45.0	45
CCGCACG	20	7.031963E-4	45.0	22
TCGCGCG	20	7.031963E-4	45.0	1
TCGCGAG	25	3.8897077E-5	45.0	1
>>END_MODULE