##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549724_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	326341
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10394035686598	33.0	31.0	34.0	30.0	34.0
2	32.29275512424121	34.0	31.0	34.0	30.0	34.0
3	32.3691568022406	34.0	31.0	34.0	30.0	34.0
4	35.88090065299794	37.0	35.0	37.0	35.0	37.0
5	35.847815628437736	37.0	35.0	37.0	35.0	37.0
6	35.49849390668044	37.0	35.0	37.0	33.0	37.0
7	35.777894901345526	37.0	35.0	37.0	35.0	37.0
8	35.83575768904306	37.0	35.0	37.0	35.0	37.0
9	37.61015931188542	39.0	37.0	39.0	35.0	39.0
10	37.19337747938506	39.0	37.0	39.0	34.0	39.0
11	37.09511216794702	39.0	37.0	39.0	33.0	39.0
12	36.9792670856558	39.0	37.0	39.0	33.0	39.0
13	36.940476372873775	39.0	37.0	39.0	33.0	39.0
14	38.06104044542365	40.0	37.0	41.0	33.0	41.0
15	38.09196515301479	40.0	37.0	41.0	33.0	41.0
16	38.11929852516233	40.0	37.0	41.0	33.0	41.0
17	38.05765135241971	40.0	37.0	41.0	33.0	41.0
18	38.04745343061399	40.0	37.0	41.0	33.0	41.0
19	38.09119295460883	40.0	37.0	41.0	34.0	41.0
20	38.047756794273475	40.0	37.0	41.0	33.0	41.0
21	37.898544773718285	40.0	37.0	41.0	33.0	41.0
22	37.963875210286176	40.0	37.0	41.0	33.0	41.0
23	37.9147854544786	40.0	37.0	41.0	33.0	41.0
24	37.87289981951395	40.0	37.0	41.0	33.0	41.0
25	37.78762092412538	40.0	36.0	41.0	33.0	41.0
26	37.70900683640732	40.0	36.0	41.0	33.0	41.0
27	37.649231938371216	40.0	36.0	41.0	33.0	41.0
28	37.536644185070216	39.0	36.0	41.0	33.0	41.0
29	37.42590419224063	39.0	36.0	41.0	32.0	41.0
30	37.3938886011871	39.0	36.0	41.0	32.0	41.0
31	37.29436999947907	39.0	36.0	41.0	32.0	41.0
32	37.1708397044809	39.0	35.0	41.0	32.0	41.0
33	37.12556191223291	39.0	35.0	41.0	31.0	41.0
34	36.96975556243316	39.0	35.0	41.0	31.0	41.0
35	36.92144413359033	39.0	35.0	41.0	31.0	41.0
36	36.78228907798898	39.0	35.0	41.0	31.0	41.0
37	36.694984693924454	39.0	35.0	41.0	30.0	41.0
38	36.62877174489261	39.0	35.0	40.0	30.0	41.0
39	36.56896926834201	39.0	35.0	40.0	30.0	41.0
40	36.432354500353924	39.0	35.0	40.0	30.0	41.0
41	36.384809141358275	39.0	35.0	40.0	30.0	41.0
42	36.33758246741905	39.0	35.0	40.0	30.0	41.0
43	36.25189602287178	38.0	35.0	40.0	30.0	41.0
44	36.11059903597771	38.0	35.0	40.0	30.0	41.0
45	36.1746486037611	38.0	35.0	40.0	30.0	41.0
46	36.03442105037369	38.0	35.0	40.0	30.0	41.0
47	35.82002261438189	38.0	35.0	40.0	29.0	41.0
48	35.73710934268143	38.0	35.0	40.0	29.0	41.0
49	35.705626936241536	38.0	35.0	40.0	29.0	41.0
50	35.59210457772698	38.0	34.0	40.0	28.0	41.0
51	34.68184506390555	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	13.0
10	14.0
11	12.0
12	8.0
13	8.0
14	11.0
15	8.0
16	19.0
17	28.0
18	71.0
19	132.0
20	221.0
21	376.0
22	606.0
23	833.0
24	1180.0
25	1667.0
26	2213.0
27	2875.0
28	3554.0
29	4316.0
30	5537.0
31	7002.0
32	9329.0
33	12768.0
34	20420.0
35	25661.0
36	29962.0
37	44636.0
38	71504.0
39	81338.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.134138830241987	4.436463699014221	55.40247777631373	15.02691969443006
2	20.4427270860848	5.124394421785801	52.73226471696783	21.700613775161564
3	21.424828630175185	4.797435811007504	51.37662751539034	22.40110804342697
4	18.827851848220114	4.939618374644927	51.26355560594593	24.968974171189032
5	17.518178837473684	5.558602811169911	50.894309939603055	26.02890841175335
6	21.236375447767823	6.3954575122341355	53.82713174256376	18.54103529743428
7	80.8497859600847	2.27614672995425	11.100964941579514	5.773102368381539
8	81.27112437603611	3.4874563723222027	8.905408759549061	6.336010492092627
9	75.2222368626682	6.24163068691951	11.504224109137375	7.031908341274924
10	36.07361624803503	32.74703454362155	17.421347608789578	13.75800159955384
11	23.803628719652142	26.29488786269577	29.47744843583859	20.424034981813502
12	23.147260074584562	22.968919014160036	33.82351589288505	20.060305018370357
13	23.63539978121045	24.136103033330166	32.14337150404025	20.08512568141913
14	19.181469689680426	27.329388584333564	31.049117334322073	22.440024391663936
15	16.77907464891019	27.351451395932475	33.72852323183418	22.14095072332315
16	19.990745876246013	27.90148954621087	29.14957054124367	22.958194036299453
17	20.11760704293975	27.14553182100931	28.485234769765366	24.25162636628557
18	20.749155024958554	25.50032021719612	30.826650650699726	22.923874107145593
19	21.31665956775275	26.88690664059986	29.8252441464603	21.97118964518709
20	23.59035487419601	27.644396505495784	29.38521362623759	19.38003499407062
21	23.903524227724986	27.792094772032932	29.373262936621508	18.93111806362057
22	21.957706815876644	25.875081586438725	28.177581119136118	23.989630478548513
23	21.416861503764466	26.059551205640723	28.598919535087532	23.924667755507276
24	22.635831844604265	26.048213371902396	27.902408830027486	23.413545953465853
25	20.895014723862463	27.802513321954642	26.544626632877875	24.757845321305016
26	20.37990935861568	27.934890191548106	27.91987522254329	23.765325227292923
27	21.26855038134957	27.13327470345436	28.449382700917138	23.14879221427893
28	18.62928654382992	27.297520078690695	29.367440805782905	24.705752571696475
29	20.284610269625944	27.640106514351555	27.974112967723947	24.10117024829856
30	22.014702412507162	28.07707275518553	26.036569110225194	23.871655722082117
31	20.668870904973634	32.18933569487132	23.45338158551944	23.68841181463561
32	20.73138220450388	31.540934176214453	23.834884369417267	23.892799249864403
33	22.57270768919627	29.34782941769499	24.214242157742973	23.865220735365767
34	19.7106707401154	28.176355407380623	27.13480684314873	24.978167009355246
35	20.648953088946836	28.02069001443276	26.350657747570793	24.979699149049615
36	23.324988279131336	27.583110917721033	26.613572919124472	22.47832788402315
37	21.599492555333224	29.97968382765267	26.02461842060912	22.396205196404985
38	19.868787556574258	31.53357990568148	24.967748459433537	23.629884078310724
39	21.736772271948666	28.63354589218027	25.93177075513037	23.697911080740695
40	21.282339638598888	28.52139326655247	26.724806260935647	23.471460833912992
41	19.849176168486338	28.987470161579452	25.73044759929031	25.432906070643895
42	22.853089253265757	27.27116727594755	25.649550623427643	24.22619284735905
43	23.291587633794098	25.466613143920007	26.564544448904677	24.677254773381218
44	20.848437677153655	25.607876423740812	26.03626268228632	27.507423216819216
45	21.720225163249484	25.371314054930274	25.484998820252436	27.423461961567806
46	21.164671310071366	27.53929172246209	27.171578195813584	24.124458771652964
47	18.737455606252357	28.33784293116709	29.167649789637217	23.757051672943334
48	18.92621521659859	27.28189225380813	28.907798897472276	24.884093632121
49	21.468034969556385	25.378668325463245	29.81972844356057	23.333568261419803
50	21.202974802430585	24.6319034384279	27.344709981277255	26.820411777864255
51	19.754183507435474	24.661626948498654	26.279872893690953	29.30431665037491
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	83.0
1	182.0
2	281.0
3	694.5
4	1108.0
5	771.5
6	435.0
7	523.5
8	612.0
9	738.0
10	864.0
11	1073.5
12	1283.0
13	1340.0
14	1397.0
15	1342.5
16	1288.0
17	1317.5
18	1347.0
19	1338.0
20	1329.0
21	1407.5
22	1486.0
23	1618.5
24	1751.0
25	2082.0
26	2707.0
27	3001.0
28	3401.5
29	3802.0
30	4740.5
31	5679.0
32	6477.0
33	7275.0
34	8262.5
35	9250.0
36	9671.0
37	10092.0
38	10914.5
39	11737.0
40	13265.0
41	14793.0
42	17070.0
43	19347.0
44	21577.5
45	23808.0
46	28230.5
47	32653.0
48	36781.0
49	40909.0
50	37098.5
51	33288.0
52	27649.0
53	22010.0
54	19255.5
55	16501.0
56	15261.0
57	14021.0
58	12821.0
59	11621.0
60	10274.0
61	8927.0
62	8091.5
63	7256.0
64	6316.0
65	5376.0
66	4435.5
67	3495.0
68	2945.0
69	2395.0
70	1995.0
71	1595.0
72	1208.0
73	821.0
74	671.5
75	381.0
76	240.0
77	191.0
78	142.0
79	102.5
80	63.0
81	44.5
82	26.0
83	19.0
84	12.0
85	7.5
86	3.0
87	3.0
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	326341.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.32955357755976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.88232921539587	30.535236837426122
2	8.960671333016784	7.4068109189764195
3	3.6104838989665287	4.47659063229762
4	2.2621997660361504	3.7398282573413684
5	1.7876626755651222	3.6941650164186277
6	1.4306302975733864	3.5476386919943717
7	1.3222192289260126	3.825271132422417
8	1.1708294652785725	3.8711887292333116
9	1.025678095836105	3.815173603366899
>10	4.426065056653561	25.96359564060983
>50	0.07734981927732831	2.2045855636792964
>100	0.03793116137638215	3.392639551914165
>500	0.002974993049128012	0.7862980858744617
>1k	0.002974993049128012	2.740977338445104
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGCT	3167	0.9704572824131813	No Hit
TCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	2421	0.7418620400133602	No Hit
GCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	1852	0.5675045427941938	No Hit
CCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	1477	0.45259406571653577	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	686	0.21020956606739577	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCC	676	0.20714528667865822	No Hit
TCCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTG	658	0.20162958377893062	No Hit
TTCCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCT	538	0.16485823111408007	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	487	0.14923040623151856	No Hit
GCCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTG	475	0.1455532709650335	No Hit
TGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTG	463	0.14187613569854846	No Hit
CGGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCT	450	0.13789257249318965	No Hit
TTCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTG	446	0.13666686073769463	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGC	384	0.11766832852752183	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCG	382	0.11705547264977431	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	341	0.10449192715595036	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTC	331	0.10142764776721282	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0642793887375474E-4	0.0	0.0	1.0648370875862978	0.0
2	3.0642793887375474E-4	0.0	0.0	3.062747249043179	0.0
3	3.0642793887375474E-4	0.0	0.0	4.141067165939922	0.0
4	3.0642793887375474E-4	0.0	0.0	5.552474252392436	0.0
5	3.0642793887375474E-4	0.0	0.0	7.490937393707808	0.0
6	3.0642793887375474E-4	0.0	0.0	8.506439583135432	0.0
7	3.0642793887375474E-4	0.0	0.0	9.388339191214099	0.0
8	3.0642793887375474E-4	0.0	0.0	10.585859576332732	0.0
9	3.0642793887375474E-4	0.0	0.0	11.225374684762258	0.0
10	3.0642793887375474E-4	0.0	0.0	12.46119856224011	0.0
11	3.0642793887375474E-4	0.0	0.0	14.714669624717704	0.0
12	3.0642793887375474E-4	0.0	0.0	16.59950787673017	0.0
13	3.0642793887375474E-4	0.0	0.0	17.24024869691519	0.0
14	3.0642793887375474E-4	0.0	0.0	17.473133930459245	0.0
15	3.0642793887375474E-4	0.0	0.0	17.843605308557613	0.0
16	3.0642793887375474E-4	0.0	0.0	18.69271712717679	0.0
17	3.0642793887375474E-4	0.0	0.0	20.077771410886157	0.0
18	0.0015321396943687737	0.0	0.0	21.659246003413607	0.0
19	0.0015321396943687737	0.0	0.0	22.520002083709983	0.0
20	0.0015321396943687737	0.0	0.0	23.447253026741965	0.0
21	0.0015321396943687737	0.0	0.0	24.376648965346064	0.0
22	0.0015321396943687737	0.0	0.0	25.317995593566238	0.0
23	0.0015321396943687737	0.0	0.0	26.164962416613296	0.0
24	0.0015321396943687737	0.0	0.0	26.793446119243367	0.0
25	0.0015321396943687737	0.0	0.0	27.31345433151213	0.0
26	0.0015321396943687737	0.0	0.0	27.812319015998604	0.0
27	0.0015321396943687737	0.0	0.0	28.3237472459789	0.0
28	0.0015321396943687737	0.0	0.0	28.840997606797796	0.0
29	0.0015321396943687737	0.0	0.0	29.368666517538404	0.0
30	0.0015321396943687737	0.0	0.0	29.921768947205532	0.0
31	0.0015321396943687737	0.0	0.0	30.44576072267965	0.0
32	0.0015321396943687737	0.0	0.0	30.942173983655135	0.0
33	0.0015321396943687737	0.0	0.0	31.43552296524188	0.0
34	0.0015321396943687737	0.0	0.0	31.936226217361593	0.0
35	0.0015321396943687737	0.0	0.0	32.44857373115851	0.0
36	0.0015321396943687737	0.0	0.0	32.916489193818734	0.0
37	0.0015321396943687737	0.0	0.0	33.413208882733095	0.0
38	0.0015321396943687737	0.0	0.0	33.89613931439813	0.0
39	0.0015321396943687737	0.0	0.0	34.38121474163528	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCACGG	45	3.8380676E-10	45.000004	2
TCGATCA	55	1.8189894E-12	45.000004	17
CCCCACG	45	3.8380676E-10	45.000004	38
TTACACG	45	3.8380676E-10	45.000004	34
CTAGACG	20	7.025854E-4	45.0	26
TGCCCCG	20	7.025854E-4	45.0	1
TAGACGG	20	7.025854E-4	45.0	27
TACGGGA	40	6.7884685E-9	45.0	4
ACGGGAA	25	3.884646E-5	45.0	5
TTGAGCG	20	7.025854E-4	45.0	1
ATCCGGC	20	7.025854E-4	45.0	44
TGTTGCG	40	6.7884685E-9	45.0	1
CGCTAGA	20	7.025854E-4	45.0	24
ACACTAC	20	7.025854E-4	45.0	32
ACGAGTT	20	7.025854E-4	45.0	12
TGCGTAG	20	7.025854E-4	45.0	1
CGGACGA	20	7.025854E-4	45.0	31
CGGGGTA	90	0.0	42.500004	6
TATCTGG	65	0.0	41.538464	2
GCCTCGA	65	0.0	41.538464	14
>>END_MODULE