FastQCFastQC Report
Sat 18 Jun 2016
SRR3549722_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549722_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1625787
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC36690.22567531909161534No Hit
CTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGCT34330.21115927240161225No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32340.1989190465909741No Hit
GCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC28120.17296238683173135No Hit
CCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC26380.16225987783147483No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT19720.1212951020029069No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAT207.034151E-445.014
CACGAAC453.8562575E-1045.018
CGGTCTA207.034151E-445.031
TATTGCG600.044.9999961
TTCGTAC750.042.033
TCGTACA750.042.034
CGAATAT4200.041.78571314
TACGCGG556.184564E-1140.9090922
CGTTTTT21750.040.241381
GCTACGA4350.039.82758310
TTGGGAT90750.039.4710775
TCGATTA403.4594086E-739.37541
GGAACGT2400.039.3749968
TTTCGCG4350.039.3103451
TTTGGGA130400.039.1679464
CTCGCGG2650.039.0566022
CCGCGTG750.039.01
TTTTGCG15100.038.8907281
CGTAGCG356.2498875E-638.57142610
CTCGACG356.2498875E-638.5714261
Produced by FastQC (version 0.11.5)