##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549722_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1625787
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28162299243382	33.0	31.0	34.0	30.0	34.0
2	32.47079968040094	34.0	31.0	34.0	31.0	34.0
3	32.54608137474343	34.0	31.0	34.0	31.0	34.0
4	36.01060224986422	37.0	35.0	37.0	35.0	37.0
5	35.97030299787119	37.0	35.0	37.0	35.0	37.0
6	35.635453475762816	37.0	35.0	37.0	35.0	37.0
7	35.98051835818591	37.0	35.0	37.0	35.0	37.0
8	36.0687291754701	37.0	35.0	37.0	35.0	37.0
9	37.866571697276456	39.0	38.0	39.0	35.0	39.0
10	37.32607407981488	39.0	37.0	39.0	34.0	39.0
11	37.18299937199645	39.0	37.0	39.0	34.0	39.0
12	37.06747747398644	39.0	37.0	39.0	33.0	39.0
13	37.041720102325826	39.0	37.0	39.0	33.0	39.0
14	38.25954937516415	40.0	38.0	41.0	33.0	41.0
15	38.299966723808225	40.0	38.0	41.0	33.0	41.0
16	38.32653847029162	40.0	38.0	41.0	34.0	41.0
17	38.25436419407954	40.0	37.0	41.0	34.0	41.0
18	38.225934885689206	40.0	37.0	41.0	34.0	41.0
19	38.26629011057414	40.0	37.0	41.0	34.0	41.0
20	38.21391978161961	40.0	37.0	41.0	34.0	41.0
21	38.08510093880687	40.0	37.0	41.0	33.0	41.0
22	38.14836691399304	40.0	37.0	41.0	34.0	41.0
23	38.099518571621005	40.0	37.0	41.0	34.0	41.0
24	38.06274315147064	40.0	37.0	41.0	34.0	41.0
25	37.98365960608616	40.0	37.0	41.0	33.0	41.0
26	37.93260187220097	40.0	37.0	41.0	33.0	41.0
27	37.86328221347569	40.0	37.0	41.0	33.0	41.0
28	37.7705025320045	40.0	36.0	41.0	33.0	41.0
29	37.6899796836855	40.0	36.0	41.0	33.0	41.0
30	37.65691938734902	40.0	36.0	41.0	33.0	41.0
31	37.62328336983873	40.0	36.0	41.0	33.0	41.0
32	37.50236285565083	40.0	36.0	41.0	33.0	41.0
33	37.44239190004595	40.0	36.0	41.0	32.0	41.0
34	37.31999025702629	40.0	36.0	41.0	32.0	41.0
35	37.28656705952256	40.0	36.0	41.0	32.0	41.0
36	37.15228932203296	39.0	35.0	41.0	31.0	41.0
37	37.14242333097755	39.0	35.0	41.0	31.0	41.0
38	37.062863708468576	39.0	35.0	41.0	31.0	41.0
39	36.974975196627845	39.0	35.0	41.0	31.0	41.0
40	36.883599143061176	39.0	35.0	41.0	31.0	41.0
41	36.82456127401683	39.0	35.0	41.0	31.0	41.0
42	36.77424410454752	39.0	35.0	41.0	31.0	41.0
43	36.722061992130584	39.0	35.0	41.0	31.0	41.0
44	36.57543884900051	39.0	35.0	41.0	30.0	41.0
45	36.607871141791634	39.0	35.0	41.0	31.0	41.0
46	36.497600854232445	39.0	35.0	41.0	30.0	41.0
47	36.315490897639116	39.0	35.0	40.0	30.0	41.0
48	36.217706870580216	39.0	35.0	40.0	30.0	41.0
49	36.18484094164857	38.0	35.0	40.0	30.0	41.0
50	36.09147815796288	38.0	35.0	40.0	29.0	41.0
51	35.24162267258872	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	23.0
10	36.0
11	27.0
12	21.0
13	19.0
14	30.0
15	43.0
16	66.0
17	121.0
18	295.0
19	485.0
20	886.0
21	1487.0
22	2351.0
23	3398.0
24	5143.0
25	7697.0
26	10594.0
27	13301.0
28	16249.0
29	19999.0
30	24591.0
31	31343.0
32	40903.0
33	56665.0
34	93919.0
35	117999.0
36	127582.0
37	194710.0
38	348364.0
39	507215.0
40	217.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.518455984701564	4.471495958572679	55.605685123574	16.40436293315176
2	18.462074060132107	4.275713854274883	55.350239607033394	21.911972478559612
3	20.654366162357064	4.1552798736857905	53.733730187287755	21.456623776669392
4	18.74212304563882	5.006006321861351	52.11414533392136	24.137725298578474
5	17.676669822061562	5.496476475700692	52.38176956760019	24.44508413463756
6	21.44143113458282	5.99346654881605	54.39611708052777	18.16898523607336
7	87.23172223667676	1.6033465638487696	7.590969788785369	3.5739614106891
8	89.23352198043163	2.252324566502254	5.1979748884694	3.3161785645967155
9	84.15721124599963	5.091134324484081	6.786928422972998	3.9647260065432928
10	36.952503618247654	39.0859319209712	12.591317312784517	11.370247147996631
11	24.732821704196184	25.10341145549817	31.561083955032238	18.60268288527341
12	25.434328113092302	22.419665060675232	32.23288167515179	19.913125151080678
13	23.651253208446125	22.857975860306425	32.64689654917895	20.8438743820685
14	19.099303906354276	26.108647688780877	31.889724791747014	22.90232361311783
15	17.548731783437805	27.660942054524977	34.535212792327656	20.255113369709562
16	21.571706502758357	26.141247285160972	32.77385044904406	19.51319576303661
17	20.947085934381317	25.813529078532426	30.414193249177167	22.825191737909087
18	20.87567436570719	26.117812480970752	31.247020673679888	21.759492479642166
19	22.767066042476657	27.67521206652532	29.760356061402877	19.797365829595144
20	25.51312072245626	26.699807539364013	29.82217227718022	17.964899460999504
21	25.294518900692402	26.840231838488066	28.85765478503642	19.00759447578311
22	22.97459630320577	25.106855941153423	29.61476503379594	22.30378272184487
23	21.918861449870125	26.267524589629517	30.145584876739694	21.668029083760665
24	22.716813457113386	25.154217618913176	30.414808335901323	21.714160588072115
25	21.32339599221792	26.843061237419167	28.24355219964239	23.589990570720516
26	20.76434366863556	28.3697064867661	28.514190358269566	22.351759486328778
27	21.006441803262053	27.68173198580134	29.757403645126946	21.554422565809666
28	19.54462669464081	27.596665491851024	30.752675473478387	22.10603234002978
29	20.616353802804426	26.536502014101476	30.17578563489559	22.6713585481985
30	22.76798867256289	25.75835579937593	29.87051809369862	21.60313743436256
31	22.252484489050534	27.479552979572354	27.389012213777082	22.87895031760003
32	22.2620183332749	29.464314821068193	26.848843052626204	21.424823793030697
33	22.914625347600882	28.201664793727595	27.259967019049853	21.623742839621674
34	20.66672940551253	27.29847144798181	28.88065902851973	23.154140117985936
35	20.53633101999216	27.848051436012224	27.902302085082486	23.71331545891313
36	21.5279738366711	29.09040360145579	26.966324616939364	22.415297944933744
37	22.086103530167236	29.05435951942044	28.028886932913107	20.830650017499217
38	21.188199930249166	28.951517019142116	27.489271349813965	22.371011700794753
39	20.418726438334172	28.95237814055593	27.57150844483318	23.05738697627672
40	21.36958900520179	27.1070564594255	28.554047977994657	22.969306557378058
41	18.730805449914413	27.666969904421673	28.934848168917576	24.667376476746337
42	20.843382312689176	29.01837694605751	26.93581631542139	23.202424425831918
43	22.04409310690761	26.88273433112702	27.707627136888163	23.365545425077208
44	20.988973340296116	26.83242023709133	28.2226392510212	23.955967171591357
45	21.69982906739936	25.901301954069012	27.399222653398013	24.99964632513361
46	21.57059934665488	27.558222571591486	27.251540330928957	23.619637750824676
47	19.98429068506514	27.587623717005975	28.54758956739105	23.880496030537827
48	20.330092441383773	26.55181767353288	29.504603001500197	23.61348688358315
49	20.846949815689264	26.880027949540747	28.94727292074546	23.32574931402453
50	20.12243916330983	26.619231178500012	28.02802581149929	25.230303846690866
51	18.594317705824935	26.54935732663627	27.55963727105703	27.296687696481765
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	244.0
1	943.5
2	1643.0
3	3970.0
4	6297.0
5	4477.0
6	2657.0
7	2640.0
8	2623.0
9	2686.0
10	2749.0
11	2836.0
12	2923.0
13	2950.5
14	2978.0
15	2989.5
16	3001.0
17	3297.5
18	3594.0
19	4252.5
20	4911.0
21	6227.5
22	7544.0
23	8730.0
24	9916.0
25	11753.5
26	17756.5
27	21922.0
28	25992.0
29	30062.0
30	35191.0
31	40320.0
32	45819.5
33	51319.0
34	57690.0
35	64061.0
36	66393.5
37	68726.0
38	73988.0
39	79250.0
40	85578.5
41	91907.0
42	96090.5
43	100274.0
44	107623.5
45	114973.0
46	122098.0
47	129223.0
48	139269.5
49	149316.0
50	142862.0
51	136408.0
52	121002.5
53	105597.0
54	94778.5
55	83960.0
56	77340.5
57	70721.0
58	67471.0
59	64221.0
60	58351.5
61	52482.0
62	46679.0
63	40876.0
64	33035.0
65	25194.0
66	20355.0
67	15516.0
68	12477.5
69	9439.0
70	7610.5
71	5782.0
72	5289.5
73	4797.0
74	3713.0
75	1950.0
76	1271.0
77	831.0
78	391.0
79	341.5
80	292.0
81	211.0
82	130.0
83	83.0
84	36.0
85	24.5
86	13.0
87	9.0
88	5.0
89	4.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1625787.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.819172653104147
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.093351059294	17.892973270814664
2	11.449039957858075	5.683113988527355
3	4.3121831847430725	3.210744569218524
4	2.1060792926995973	2.0908458234655503
5	1.2294935798252287	1.5257506716782716
6	0.7713569107409975	1.1486664206907515
7	0.5252185898725626	0.9124843603866919
8	0.41577086950683195	0.8255271195537036
9	0.3367421643392517	0.7521895724684272
>10	4.96529504364919	32.46798203651739
>50	1.677321217303827	27.20004256382882
>100	0.11387468721590535	4.628540865568982
>500	0.002513789971476321	0.47048440254778845
>1k	0.0017596529800334249	1.1906543347330585
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	3669	0.22567531909161534	No Hit
CTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGCT	3433	0.21115927240161225	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3234	0.1989190465909741	No Hit
GCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	2812	0.17296238683173135	No Hit
CCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	2638	0.16225987783147483	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1972	0.1212951020029069	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0754336207633595E-4	0.0	0.0	0.22905829607445502	0.0
2	3.0754336207633595E-4	0.0	0.0	0.8659190902621315	0.0
3	3.0754336207633595E-4	0.0	0.0	1.2483185066678477	0.0
4	3.0754336207633595E-4	0.0	0.0	1.7194749373687943	0.0
5	3.0754336207633595E-4	0.0	0.0	2.4483527054897105	0.0
6	3.0754336207633595E-4	0.0	0.0	2.899518817655695	0.0
7	3.0754336207633595E-4	0.0	0.0	3.320730206355445	0.0
8	3.0754336207633595E-4	0.0	0.0	3.997756163630291	0.0
9	3.0754336207633595E-4	0.0	0.0	4.3303335553796405	0.0
10	3.0754336207633595E-4	0.0	0.0	4.974821424946811	0.0
11	3.0754336207633595E-4	0.0	0.0	6.2164354863213935	0.0
12	3.0754336207633595E-4	0.0	0.0	7.333309960037815	0.0
13	3.0754336207633595E-4	0.0	0.0	7.669823906821742	0.0
14	3.0754336207633595E-4	0.0	0.0	7.7892737486521915	0.0
15	3.0754336207633595E-4	0.0	0.0	7.962605187518414	0.0
16	3.0754336207633595E-4	0.0	0.0	8.377296656942146	0.0
17	3.0754336207633595E-4	0.0	0.0	9.02965763657847	0.0
18	3.0754336207633595E-4	0.0	0.0	9.72390602212959	0.0
19	3.690520344916031E-4	0.0	0.0	10.252634570211226	0.0
20	4.3056070690687036E-4	0.0	0.0	10.799508176655367	0.0
21	4.3056070690687036E-4	0.0	0.0	11.550283032156118	0.0
22	4.3056070690687036E-4	0.0	0.0	12.401870601745493	0.0
23	4.3056070690687036E-4	0.0	0.0	13.201790886506043	0.0
24	4.3056070690687036E-4	0.0	0.0	13.790182846830488	0.0
25	4.3056070690687036E-4	0.0	0.0	14.300458793187545	0.0
26	4.3056070690687036E-4	0.0	0.0	14.761404784267558	0.0
27	4.3056070690687036E-4	0.0	0.0	15.213616543864601	0.0
28	4.920693793221375E-4	0.0	0.0	15.702056911514239	0.0
29	5.535780517374047E-4	0.0	0.0	16.232384685078674	0.0
30	6.150867241526719E-4	0.0	0.0	16.814810304178838	0.0
31	6.150867241526719E-4	0.0	0.0	17.354610413295223	0.0
32	6.150867241526719E-4	0.0	0.0	17.862979590807406	0.0
33	6.150867241526719E-4	0.0	0.0	18.382604855371582	0.0
34	6.150867241526719E-4	0.0	0.0	18.901676541884022	0.0
35	6.150867241526719E-4	0.0	0.0	19.438954795431382	0.0
36	6.150867241526719E-4	0.0	0.0	19.977586239771878	0.0
37	6.150867241526719E-4	0.0	0.0	20.526305106388474	0.0
38	6.150867241526719E-4	0.0	0.0	21.056509862608078	0.0
39	6.150867241526719E-4	0.0	0.0	21.631492932345996	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGAT	20	7.034151E-4	45.0	14
CACGAAC	45	3.8562575E-10	45.0	18
CGGTCTA	20	7.034151E-4	45.0	31
TATTGCG	60	0.0	44.999996	1
TTCGTAC	75	0.0	42.0	33
TCGTACA	75	0.0	42.0	34
CGAATAT	420	0.0	41.785713	14
TACGCGG	55	6.184564E-11	40.909092	2
CGTTTTT	2175	0.0	40.24138	1
GCTACGA	435	0.0	39.827583	10
TTGGGAT	9075	0.0	39.471077	5
TCGATTA	40	3.4594086E-7	39.375	41
GGAACGT	240	0.0	39.374996	8
TTTCGCG	435	0.0	39.310345	1
TTTGGGA	13040	0.0	39.167946	4
CTCGCGG	265	0.0	39.056602	2
CCGCGTG	75	0.0	39.0	1
TTTTGCG	1510	0.0	38.890728	1
CGTAGCG	35	6.2498875E-6	38.571426	10
CTCGACG	35	6.2498875E-6	38.571426	1
>>END_MODULE