FastQCFastQC Report
Sat 18 Jun 2016
SRR3549721_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549721_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2461156
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101940.4141956056422267No Hit
CTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCT56320.22883555532440852No Hit
GCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC56240.22851050481968635No Hit
TCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC49630.20165320686701696No Hit
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC43960.17861525234483308No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC29700.12067499987810607No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCG24950.10137512616022716No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA650.041.5384645
AAACACG5050.040.54455640
CGTTTTT53300.040.3986851
TATTGCG1150.039.1304361
CGACCAA5650.039.0265529
TCAAGCG5900.038.5169517
CGTTTGG16900.038.4763342
TTACACG22050.037.755134
GGGCGAT43600.037.46567
ACGACCA5900.037.37288328
TGGGCGA29450.037.3599326
TTTGGGA106750.037.2435574
TTGGGAC37850.037.212685
TTTGGGC58250.037.042924
AACACGT5300.036.93396441
ACACGCG22200.036.8918936
GCGAGAC6050.036.81818421
TTGGGAG63650.036.79895
GTTTGCG6300.036.7857131
CCCGATC3400.036.3970641