##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549721_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2461156
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.121128851645324	33.0	31.0	34.0	30.0	34.0
2	32.30965164337409	34.0	31.0	34.0	30.0	34.0
3	32.38197131754346	34.0	31.0	34.0	30.0	34.0
4	35.910338881403696	37.0	35.0	37.0	35.0	37.0
5	35.86898067412224	37.0	35.0	37.0	35.0	37.0
6	35.481524129311595	37.0	35.0	37.0	33.0	37.0
7	35.82826484790074	37.0	35.0	37.0	35.0	37.0
8	35.90098717838284	37.0	35.0	37.0	35.0	37.0
9	37.68553151445906	39.0	38.0	39.0	35.0	39.0
10	37.17810979880999	39.0	37.0	39.0	34.0	39.0
11	37.077775240578006	39.0	37.0	39.0	33.0	39.0
12	36.83679823627596	39.0	35.0	39.0	33.0	39.0
13	36.70929758211182	39.0	35.0	39.0	33.0	39.0
14	37.6441009834403	40.0	37.0	41.0	32.0	41.0
15	37.737594447487275	40.0	37.0	41.0	33.0	41.0
16	37.84307130470397	40.0	36.0	41.0	33.0	41.0
17	37.798730352728555	40.0	36.0	41.0	33.0	41.0
18	37.788707014102314	40.0	36.0	41.0	33.0	41.0
19	37.80829740170879	40.0	36.0	41.0	33.0	41.0
20	37.70723554297249	40.0	36.0	41.0	33.0	41.0
21	37.54740211510364	39.0	36.0	41.0	33.0	41.0
22	37.6654035745804	39.0	36.0	41.0	33.0	41.0
23	37.63124686123106	39.0	36.0	41.0	33.0	41.0
24	37.5767009486599	39.0	35.0	41.0	33.0	41.0
25	37.49749995530556	39.0	35.0	41.0	33.0	41.0
26	37.430008093757564	39.0	35.0	41.0	33.0	41.0
27	37.351764374139634	39.0	35.0	41.0	32.0	41.0
28	37.23401401617777	39.0	35.0	41.0	32.0	41.0
29	37.135385160469305	39.0	35.0	41.0	32.0	41.0
30	37.08442496127836	39.0	35.0	41.0	32.0	41.0
31	36.98609555834738	39.0	35.0	41.0	31.0	41.0
32	36.853711832976046	39.0	35.0	41.0	31.0	41.0
33	36.78100372345353	39.0	35.0	41.0	31.0	41.0
34	36.59275153626995	39.0	35.0	41.0	30.0	41.0
35	36.49651992803382	39.0	35.0	41.0	30.0	41.0
36	36.33600755092323	39.0	35.0	40.0	30.0	41.0
37	36.278247295173486	39.0	35.0	40.0	30.0	41.0
38	36.20177388186689	39.0	35.0	40.0	30.0	41.0
39	36.0999981309596	38.0	35.0	40.0	30.0	41.0
40	35.92716268290186	38.0	35.0	40.0	29.0	41.0
41	35.7740992444201	38.0	35.0	40.0	28.0	41.0
42	35.73666886617508	38.0	35.0	40.0	28.0	41.0
43	35.71438787301577	38.0	35.0	40.0	29.0	41.0
44	35.60424938524823	38.0	34.0	40.0	28.0	41.0
45	35.64804872182015	38.0	34.0	40.0	29.0	41.0
46	35.547249747679544	38.0	34.0	40.0	28.0	41.0
47	35.30274025701743	38.0	34.0	40.0	27.0	41.0
48	35.182672288956894	37.0	34.0	40.0	27.0	41.0
49	35.17968019906093	37.0	34.0	40.0	27.0	41.0
50	35.061426825443	37.0	34.0	40.0	27.0	41.0
51	34.106531646104514	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	27.0
10	45.0
11	51.0
12	38.0
13	41.0
14	48.0
15	87.0
16	165.0
17	330.0
18	695.0
19	1225.0
20	2097.0
21	3426.0
22	5255.0
23	7986.0
24	11686.0
25	17172.0
26	22921.0
27	28272.0
28	32938.0
29	39094.0
30	48111.0
31	61085.0
32	79043.0
33	111041.0
34	177887.0
35	189386.0
36	216123.0
37	318159.0
38	509496.0
39	577051.0
40	167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.84596181631721	4.490207040918983	50.5321076762302	18.13172346653361
2	21.118490660486373	5.29531650980271	50.24281272702746	23.343380102683454
3	21.392711392532615	5.580263908504784	49.925766590984075	23.101258107978527
4	19.383127278400885	5.057420171659172	50.15598361095355	25.403468938986396
5	18.466728642962902	5.806377165852145	49.98195969698792	25.744934494197036
6	21.862937578926324	6.977249715174495	52.63412802764229	18.525684678256884
7	82.75351095176413	2.7493178002532144	9.946220393993716	4.5509508539889385
8	84.16886211195065	3.393689794551828	8.048534916112592	4.388913177384937
9	78.90158120818022	5.221408151291507	10.014806050490096	5.862204590038178
10	40.72679667603354	24.854133586005926	18.628888213506173	15.790181524454361
11	30.292431686573302	24.30699232393233	25.281818787594123	20.118757201900245
12	26.976185174771526	21.431392402594554	30.678997999314145	20.913424423319775
13	25.239034014910068	21.634061392288828	32.25927165933407	20.867632933467036
14	20.69889109020314	23.418141718769554	34.05704473832622	21.82592245270109
15	19.31726391988155	23.050347072676416	36.048913599950595	21.58347540749144
16	24.253358990653172	22.819114269879684	31.017781887860828	21.90974485160632
17	24.210858637160747	23.470434218716733	29.333004490572723	22.985702653549794
18	25.24427545429871	22.146828563488054	30.32518052492406	22.283715457289176
19	25.21404575735955	24.52400416714747	27.935653002085196	22.32629707340778
20	25.411717095543718	27.15154992206914	28.090823986776947	19.34590899561019
21	25.433129797542293	25.589235302435114	29.881242798099755	19.096392101922834
22	23.797353763841055	22.604174623632147	30.551781358028503	23.04669025449829
23	22.744433916419766	24.535299672186568	30.126777823104266	22.593488588289407
24	22.849994067828288	24.78319131335031	29.54810666207262	22.818707956748778
25	23.72312035482513	25.54901030247575	26.414294746046167	24.313574596652956
26	22.024487679773245	24.970176616191743	27.37705370972015	25.628281994314868
27	21.69456141748024	23.88312646577462	29.439539793495413	24.982772323249726
28	19.37500101578283	25.031651792897318	28.93168901118011	26.661658180139742
29	21.47263318538118	25.058712247415443	27.995827976771892	25.47282659043149
30	23.304495936056068	22.8578765425678	29.08889156152637	24.748735959849764
31	22.418001947052524	25.335370858247103	27.620841588261776	24.625785606438598
32	23.453816011662813	25.23976537854569	26.64077368521134	24.665644924580157
33	23.075904168610197	24.7051385609039	26.28415265021803	25.934804620267876
34	20.392937302633396	25.376367853155184	28.81003885978784	25.42065598442358
35	20.264217302763416	26.790012498191906	27.2039236846425	25.741846514402177
36	21.68099055890809	27.76508274973224	24.89825919202196	25.65566749933771
37	20.531652605523583	28.230148759363487	26.10439972110667	25.13379891400626
38	21.468732579324513	27.312693709785158	26.438511008647968	24.78006270224236
39	21.181550458402473	25.458443105597535	26.944330225308754	26.415676210691235
40	22.857429598123808	25.257074317922147	27.485783103549714	24.39971298040433
41	19.654869500348617	27.21465035129833	26.324702700682117	26.80577744767093
42	20.213915737157663	26.334779266328507	27.94093507278693	25.5103699237269
43	20.624576418561034	24.375090404671624	28.66335981953196	26.336973357235383
44	21.9637032353902	23.54759308227516	27.712221411401792	26.776482270932846
45	21.754573866914573	22.378630204668053	26.574097700430205	29.292698227987174
46	21.068351620132976	23.6885837386984	27.680203936686667	27.562860704481956
47	18.659686748828598	25.98234325658349	29.555095247924147	25.80287474666376
48	19.4097814197881	26.183793306885057	28.41189262281627	25.99453265051057
49	21.654783361964867	23.963251415188637	29.048950980758637	25.333014242087863
50	19.94713053540694	24.490158283343273	27.579763330727513	27.982947850522276
51	18.72595642047883	24.96672295457907	26.50579646312546	29.801524161816644
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	462.0
1	1614.5
2	2767.0
3	9738.5
4	16710.0
5	11045.0
6	5380.0
7	5332.0
8	5284.0
9	5577.0
10	5870.0
11	5753.5
12	5637.0
13	5704.5
14	5772.0
15	5572.0
16	5372.0
17	5543.5
18	5715.0
19	5750.5
20	5786.0
21	6205.5
22	6625.0
23	8059.5
24	9494.0
25	11621.5
26	16000.0
27	18251.0
28	21683.0
29	25115.0
30	29928.0
31	34741.0
32	40470.0
33	46199.0
34	53919.5
35	61640.0
36	67351.0
37	73062.0
38	77490.5
39	81919.0
40	90146.0
41	98373.0
42	106128.0
43	113883.0
44	128263.5
45	142644.0
46	169540.0
47	196436.0
48	218098.0
49	239760.0
50	239522.0
51	239284.0
52	215495.5
53	191707.0
54	178938.5
55	166170.0
56	156344.0
57	146518.0
58	138089.5
59	129661.0
60	117301.0
61	104941.0
62	91928.0
63	78915.0
64	69892.0
65	60869.0
66	51099.0
67	41329.0
68	34566.0
69	27803.0
70	23025.0
71	18247.0
72	15070.5
73	11894.0
74	10154.0
75	6401.5
76	4389.0
77	3417.5
78	2446.0
79	1745.5
80	1045.0
81	783.5
82	522.0
83	328.0
84	134.0
85	127.5
86	121.0
87	97.5
88	74.0
89	45.5
90	17.0
91	12.0
92	7.0
93	4.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2461156.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.364656618093186
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.37408367091402	19.80491947703707
2	11.100660871380963	6.075315459584862
3	4.349700545001897	3.570841854165291
4	2.1843817944199655	2.390994309084663
5	1.2762772168026075	1.7462443893649513
6	0.8305957395490681	1.3637380320726846
7	0.575395917521697	1.1021858191703737
8	0.46844642164477845	1.0255100377827084
9	0.3623347997686884	0.8923650638810134
>10	5.437991073996519	38.122155894413865
>50	0.9438965280561916	16.16020845108763
>100	0.08740580956746531	4.329106450243915
>500	0.005537213913801729	0.993915255519233
>1k	0.002843434171952239	1.544070078042734
>5k	2.993088602054989E-4	0.4609604497785119
>10k+	1.4965443010274944E-4	0.4174689787706246
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10194	0.4141956056422267	No Hit
CTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCT	5632	0.22883555532440852	No Hit
GCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	5624	0.22851050481968635	No Hit
TCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	4963	0.20165320686701696	No Hit
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	4396	0.17861525234483308	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC	2970	0.12067499987810607	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCG	2495	0.10137512616022716	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.03156565451357E-4	0.0	0.0	0.2527673987345784	0.0
2	2.03156565451357E-4	0.0	0.0	0.9450843424797127	0.0
3	2.03156565451357E-4	0.0	0.0	1.3912974228370734	0.0
4	2.03156565451357E-4	0.0	0.0	1.9622080030684768	0.0
5	2.03156565451357E-4	0.0	0.0	2.990749062635607	0.0
6	2.03156565451357E-4	0.0	0.0	3.754252066914897	0.0
7	2.03156565451357E-4	0.0	0.0	4.445228177328052	0.0
8	2.03156565451357E-4	0.0	0.0	5.455850827822373	0.0
9	2.03156565451357E-4	0.0	0.0	5.986495776781317	0.0
10	2.03156565451357E-4	0.0	0.0	6.974771205075989	0.0
11	2.03156565451357E-4	0.0	0.0	8.48857203688023	0.0
12	2.03156565451357E-4	0.0	0.0	9.869264687000742	0.0
13	2.03156565451357E-4	0.0	0.0	10.316777969376991	0.0
14	2.03156565451357E-4	0.0	0.0	10.49900940858686	0.0
15	2.03156565451357E-4	0.0	0.0	10.764169357813971	0.0
16	2.4378787854162842E-4	0.0	0.0	11.29241705930059	0.0
17	2.4378787854162842E-4	0.0	0.0	11.987578194962042	0.0
18	2.4378787854162842E-4	0.0	0.0	12.736738345720466	0.0
19	2.4378787854162842E-4	0.0	0.0	13.290055567383781	0.0
20	2.4378787854162842E-4	0.0	0.0	13.87514647588369	0.0
21	2.4378787854162842E-4	0.0	0.0	14.710973217463664	0.0
22	2.4378787854162842E-4	0.0	0.0	15.612460160997514	0.0
23	2.4378787854162842E-4	0.0	0.0	16.46791182680009	0.0
24	2.844191916318998E-4	0.0	0.0	17.13459041198526	0.0
25	2.844191916318998E-4	0.0	0.0	17.731098719463535	0.0
26	2.844191916318998E-4	0.0	0.0	18.263612708824635	0.0
27	2.844191916318998E-4	0.0	0.0	18.828469223405587	0.0
28	2.844191916318998E-4	0.0	0.0	19.377926470325328	0.0
29	2.844191916318998E-4	0.0	0.0	20.017382075740016	0.0
30	2.844191916318998E-4	0.0	0.0	20.758294069941115	0.0
31	2.844191916318998E-4	0.0	0.0	21.352201973381614	0.0
32	2.844191916318998E-4	0.0	0.0	21.930710609160897	0.0
33	2.844191916318998E-4	0.0	0.0	22.506375053023863	0.0
34	2.844191916318998E-4	0.0	0.0	23.137257451376506	0.0
35	2.844191916318998E-4	0.0	0.0	23.804829925449667	0.0
36	2.844191916318998E-4	0.0	0.0	24.39410585919787	0.0
37	2.844191916318998E-4	0.0	0.0	24.99244257576521	0.0
38	2.844191916318998E-4	0.0	0.0	25.695242398287633	0.0
39	2.844191916318998E-4	0.0	0.0	26.827596462800408	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	65	0.0	41.538464	5
AAACACG	505	0.0	40.544556	40
CGTTTTT	5330	0.0	40.398685	1
TATTGCG	115	0.0	39.130436	1
CGACCAA	565	0.0	39.02655	29
TCAAGCG	590	0.0	38.51695	17
CGTTTGG	1690	0.0	38.476334	2
TTACACG	2205	0.0	37.7551	34
GGGCGAT	4360	0.0	37.4656	7
ACGACCA	590	0.0	37.372883	28
TGGGCGA	2945	0.0	37.359932	6
TTTGGGA	10675	0.0	37.243557	4
TTGGGAC	3785	0.0	37.21268	5
TTTGGGC	5825	0.0	37.04292	4
AACACGT	530	0.0	36.933964	41
ACACGCG	2220	0.0	36.89189	36
GCGAGAC	605	0.0	36.818184	21
TTGGGAG	6365	0.0	36.7989	5
GTTTGCG	630	0.0	36.785713	1
CCCGATC	340	0.0	36.39706	41
>>END_MODULE