SRR3549717_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549717_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1056422
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	3482	0.32960313208168707	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	2763	0.26154320905850126	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2482	0.23494399018574016	No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	2175	0.20588363362368448	TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	2064	0.19537646887323434	TruSeq Adapter, Index 13 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCCTA	45	3.8562575E-10	45.000004	24
TCGCCGA	20	7.0330274E-4	45.0	34
GTCGCGA	20	7.0330274E-4	45.0	16
GATCGAC	35	1.2118107E-7	45.0	9
CGGTTCA	25	3.8905906E-5	45.0	41
CGCATCG	35	1.2118107E-7	45.0	21
CGTTACA	25	3.8905906E-5	45.0	10
ATCCGAT	25	3.8905906E-5	45.0	43
GAACGAT	20	7.0330274E-4	45.0	14
CTCGACG	25	3.8905906E-5	45.0	1
CGTCGCG	20	7.0330274E-4	45.0	33
CCGAATA	20	7.0330274E-4	45.0	25
TTACCCG	25	3.8905906E-5	45.0	1
GCTCGAC	20	7.0330274E-4	45.0	21
CGATCCA	20	7.0330274E-4	45.0	35
TTCGATG	25	3.8905906E-5	45.0	1
GCGAGAC	30	2.165334E-6	44.999996	21
GGACGTA	30	2.165334E-6	44.999996	8
TTATGCG	100	0.0	42.75	1
TATGCGG	140	0.0	41.785713	2