##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549714_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	669859
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.113190984968476	33.0	31.0	34.0	30.0	34.0
2	32.30003329058802	34.0	31.0	34.0	30.0	34.0
3	32.361046130603604	34.0	31.0	34.0	30.0	34.0
4	35.870426462882484	37.0	35.0	37.0	35.0	37.0
5	35.83814354961268	37.0	35.0	37.0	35.0	37.0
6	35.49107498742273	37.0	35.0	37.0	33.0	37.0
7	35.83009558728031	37.0	35.0	37.0	35.0	37.0
8	35.90970637104226	37.0	35.0	37.0	35.0	37.0
9	37.660631565747416	39.0	38.0	39.0	35.0	39.0
10	37.19579941450365	39.0	37.0	39.0	34.0	39.0
11	37.10235288321871	39.0	37.0	39.0	33.0	39.0
12	36.871687922383664	39.0	35.0	39.0	33.0	39.0
13	36.75813566735686	39.0	35.0	39.0	33.0	39.0
14	37.886880671902595	40.0	37.0	41.0	33.0	41.0
15	37.93284855469584	40.0	37.0	41.0	33.0	41.0
16	37.969141267042765	40.0	36.0	41.0	33.0	41.0
17	37.87562158603527	40.0	36.0	41.0	33.0	41.0
18	37.85181508347279	40.0	36.0	41.0	33.0	41.0
19	37.88406963256447	40.0	36.0	41.0	33.0	41.0
20	37.834148679050365	40.0	36.0	41.0	33.0	41.0
21	37.68352145750076	39.0	36.0	41.0	33.0	41.0
22	37.76782576631798	39.0	36.0	41.0	33.0	41.0
23	37.72631105949162	39.0	36.0	41.0	33.0	41.0
24	37.71975296293698	39.0	36.0	41.0	33.0	41.0
25	37.62966833318654	39.0	36.0	41.0	33.0	41.0
26	37.529990639821214	39.0	35.0	41.0	33.0	41.0
27	37.3886310402637	39.0	35.0	41.0	32.0	41.0
28	37.28856221981044	39.0	35.0	41.0	32.0	41.0
29	37.20992178951093	39.0	35.0	41.0	32.0	41.0
30	37.16819659062579	39.0	35.0	41.0	32.0	41.0
31	37.094869218746034	39.0	35.0	41.0	32.0	41.0
32	36.987982545580486	39.0	35.0	41.0	31.0	41.0
33	36.93958131487373	39.0	35.0	41.0	31.0	41.0
34	36.815465642769595	39.0	35.0	41.0	31.0	41.0
35	36.7449239317528	39.0	35.0	41.0	31.0	41.0
36	36.55360755024565	39.0	35.0	41.0	30.0	41.0
37	36.50569448197307	39.0	35.0	40.0	30.0	41.0
38	36.50379258918668	39.0	35.0	40.0	30.0	41.0
39	36.4741818800673	39.0	35.0	40.0	30.0	41.0
40	36.28266247075877	39.0	35.0	40.0	30.0	41.0
41	36.174245624825524	38.0	35.0	40.0	30.0	41.0
42	36.16046361995584	38.0	35.0	40.0	30.0	41.0
43	36.13215318447614	38.0	35.0	40.0	30.0	41.0
44	35.99765323747236	38.0	35.0	40.0	30.0	41.0
45	36.01931749816006	38.0	35.0	40.0	30.0	41.0
46	35.88316048601273	38.0	35.0	40.0	29.0	41.0
47	35.645867264603446	38.0	34.0	40.0	28.0	41.0
48	35.567098449076596	38.0	34.0	40.0	28.0	41.0
49	35.57812315726145	38.0	34.0	40.0	28.0	41.0
50	35.461029858522465	38.0	34.0	40.0	28.0	41.0
51	34.44901389695443	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	15.0
10	26.0
11	19.0
12	13.0
13	12.0
14	12.0
15	23.0
16	40.0
17	71.0
18	123.0
19	258.0
20	450.0
21	757.0
22	1167.0
23	1771.0
24	2484.0
25	3787.0
26	5513.0
27	6865.0
28	8183.0
29	9667.0
30	12257.0
31	15500.0
32	20294.0
33	27621.0
34	47961.0
35	57163.0
36	56532.0
37	83037.0
38	140737.0
39	167440.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.53426765931338	4.676655833541089	54.227830035873225	16.561246471272312
2	21.329414100579374	4.648440940556147	51.9981070643225	22.024037894541987
3	22.23676923053956	4.886998607169569	50.51391412222572	22.362318040065148
4	20.37921413312354	5.09136997487531	48.3424123584217	26.187003533579457
5	18.047380120293973	6.289084717828677	50.17652968759098	25.487005474286377
6	22.429197786399826	6.516147428040826	52.02691909789971	19.027735687659643
7	81.3068123291618	2.945694541687131	10.459216043973434	5.288277085177627
8	81.83393818699159	3.6655475256733134	8.895901973400372	5.604612313934724
9	75.87671435331913	5.789576612391563	11.695595640276537	6.63811339401277
10	38.08532840493298	29.59309347190976	17.82061598037796	14.5009621427793
11	29.247498354131242	26.16222219900009	26.261048967021416	18.329230479847254
12	29.76178568922714	20.092735933980137	30.97174181432212	19.17373656247061
13	25.68316615884835	22.409492146854788	30.693623583470554	21.213718110826306
14	19.388408605393074	26.361517871671502	31.216270886858275	23.033802636077144
15	17.56429338114439	26.141172993122435	35.43551702671756	20.859016599015614
16	20.273520248291057	24.920617622514587	33.900865704573654	20.904996424620702
17	19.85447683766285	23.62109040857852	30.900682083841524	25.6237506699171
18	21.24357514044	24.772377470482592	31.52051401862183	22.463533370455576
19	23.280123130390127	26.644711797557395	29.21838775025789	20.85677732179459
20	27.184228322676862	25.52641675337646	28.84547345038284	18.443881473563838
21	25.412362900252145	26.529314378100466	28.744556690288555	19.31376603135884
22	21.883411285061484	25.477749795106135	28.57556590267504	24.063273017157343
23	21.657990711478085	26.572607071040323	28.410605814059377	23.358796403422215
24	23.884130839475173	22.805396359532377	28.791282941633984	24.519189859358463
25	23.02156125393553	24.338853400491743	27.30962784705438	25.329957498518347
26	21.725616883553105	26.13863514560527	28.418965782351215	23.716782188490413
27	20.769893365618735	26.99732331729513	29.807616229684157	22.425167087401977
28	18.911442557314302	26.470645314909557	31.39765234176147	23.22025978601467
29	22.156901676322928	24.5995052690193	30.281148719357358	22.962444335300415
30	23.06769036468869	24.05461447856937	30.517168538453614	22.360526618288326
31	22.782406446729834	26.813851870318977	27.74001692893579	22.663724754015398
32	23.600936913589276	26.835647501936975	28.135771856465315	21.42764372800843
33	23.20324127913486	25.83319773265717	29.049695532940518	21.91386545526745
34	21.170126847590314	24.608313092755342	30.722734187343903	23.49882587231044
35	21.692475580681904	23.879204429588913	29.581747800656554	24.846572189072628
36	22.04001140538531	26.408094837868866	29.23704839376645	22.314845362979373
37	20.652256668940776	26.788921250591542	30.78468752379232	21.77413455667536
38	20.40518974888745	26.59813483136003	29.309750260875795	23.68692515887672
39	23.1429300793152	22.638495563991825	31.24762076795266	22.97095358874032
40	23.77560053682939	23.288184528385823	31.31897906872939	21.617235866055392
41	18.619590092840433	25.75900301406714	30.776626125796625	24.844780767295806
42	20.859613739607887	25.140813215915585	29.657883226171478	24.341689818305046
43	21.588722402774316	23.263253908658392	30.775581129760145	24.372442558807155
44	21.714868352892175	22.90287956122109	29.049994103236653	26.33225798265008
45	23.09874167548693	22.146302430810067	28.13233829805974	26.622617595643263
46	22.50906534061646	23.98833187282697	29.732675085353783	23.76992770120279
47	18.998027943194014	24.858664286066173	32.63074766480707	23.512560105932742
48	19.487981799154745	23.25832749877213	32.79436418709012	24.459326514983005
49	21.4912392010856	21.487357787235823	33.21266117197798	23.80874183970059
50	20.236348246422008	21.38076819151493	31.476773470237767	26.906110091825298
51	18.768278100316635	22.03612999153553	29.708640176514763	29.486951731633077
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	226.0
1	595.0
2	964.0
3	2081.0
4	3198.0
5	2262.5
6	1327.0
7	1314.0
8	1301.0
9	1373.5
10	1446.0
11	1551.0
12	1656.0
13	1605.0
14	1554.0
15	1746.0
16	1938.0
17	1801.0
18	1664.0
19	1680.0
20	1696.0
21	2059.0
22	2422.0
23	3189.0
24	3956.0
25	3724.0
26	4421.5
27	5351.0
28	6795.5
29	8240.0
30	9340.0
31	10440.0
32	12547.0
33	14654.0
34	16392.5
35	18131.0
36	19597.5
37	21064.0
38	22666.5
39	24269.0
40	28494.5
41	32720.0
42	38534.5
43	44349.0
44	51114.0
45	57879.0
46	65790.5
47	73702.0
48	71647.5
49	69593.0
50	59255.5
51	48918.0
52	45540.5
53	42163.0
54	36939.0
55	31715.0
56	30138.5
57	28562.0
58	28371.0
59	28180.0
60	27878.0
61	27576.0
62	24153.0
63	20730.0
64	17117.5
65	13505.0
66	11085.0
67	8665.0
68	6890.0
69	5115.0
70	3995.5
71	2876.0
72	2486.0
73	2096.0
74	1675.0
75	915.0
76	576.0
77	477.5
78	379.0
79	301.5
80	224.0
81	124.5
82	25.0
83	17.0
84	9.0
85	25.0
86	41.0
87	23.5
88	6.0
89	6.5
90	7.0
91	5.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	669859.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.54854975626492
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.73256311754837	19.210309846756648
2	12.204192337648543	6.724155996974759
3	5.250104075952549	4.338982600858441
4	2.824242273657575	3.112151151984099
5	1.6262558067321853	2.2400494504088178
6	1.0470691047449598	1.7307141198188574
7	0.7564474138822571	1.45873204535333
8	0.5539969814036979	1.2209450725616273
9	0.42846464864184275	1.0623221722719334
>10	4.26571272306089	28.800031273084702
>50	1.1872974921944996	21.79655582644156
>100	0.11818260751797324	5.674215937585092
>500	0.002188566805888393	0.40485971598760934
>1k	0.0032828502088325896	2.225974789912515
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	3459	0.516377327168852	No Hit
TCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	3109	0.46412752534488605	No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	3085	0.46054468179124264	No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	2199	0.3282780406025746	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1767	0.26378685663699375	No Hit
TCCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG	1149	0.17152863513067676	No Hit
GCCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG	790	0.11793526697409455	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.492851480684741E-4	0.0	0.0	0.5556393211108607	0.0
2	1.492851480684741E-4	0.0	0.0	1.977729641611145	0.0
3	1.492851480684741E-4	0.0	0.0	2.7923786946208082	0.0
4	1.492851480684741E-4	0.0	0.0	3.6455433158321378	0.0
5	1.492851480684741E-4	0.0	0.0	5.004933874143663	0.0
6	1.492851480684741E-4	0.0	0.0	5.856008503282034	0.0
7	1.492851480684741E-4	0.0	0.0	6.6442340850835775	0.0
8	1.492851480684741E-4	0.0	0.0	7.835081711225795	0.0
9	1.492851480684741E-4	0.0	0.0	8.422070913431035	0.0
10	1.492851480684741E-4	0.0	0.0	9.532901700208551	0.0
11	1.492851480684741E-4	0.0	0.0	11.62976088997834	0.0
12	1.492851480684741E-4	0.0	0.0	13.432528338053233	0.0
13	1.492851480684741E-4	0.0	0.0	14.021010391739157	0.0
14	1.492851480684741E-4	0.0	0.0	14.253148796985634	0.0
15	1.492851480684741E-4	0.0	0.0	14.59695249298733	0.0
16	1.492851480684741E-4	0.0	0.0	15.375325255016355	0.0
17	1.492851480684741E-4	0.0	0.0	16.50392097441402	0.0
18	1.492851480684741E-4	0.0	0.0	17.861072255504517	0.0
19	1.492851480684741E-4	0.0	0.0	18.623023651246008	0.0
20	1.492851480684741E-4	0.0	0.0	19.473501139792106	0.0
21	1.492851480684741E-4	0.0	0.0	20.57776338005461	0.0
22	1.492851480684741E-4	0.0	0.0	21.663066406512414	0.0
23	1.492851480684741E-4	0.0	0.0	22.66342618371926	0.0
24	1.492851480684741E-4	0.0	0.0	23.446128215042272	0.0
25	1.492851480684741E-4	0.0	0.0	24.20136177912068	0.0
26	1.492851480684741E-4	0.0	0.0	24.837764365336586	0.0
27	1.492851480684741E-4	0.0	0.0	25.481631208955914	0.0
28	1.492851480684741E-4	0.0	0.0	26.129080896128887	0.0
29	1.492851480684741E-4	0.0	0.0	26.78235270407653	0.0
30	1.492851480684741E-4	0.0	0.0	27.546543377039047	0.0
31	2.985702961369482E-4	0.0	0.0	28.26639636102523	0.0
32	2.985702961369482E-4	0.0	0.0	28.92414672341493	0.0
33	2.985702961369482E-4	0.0	0.0	29.61264982630673	0.0
34	2.985702961369482E-4	0.0	0.0	30.23875173730591	0.0
35	2.985702961369482E-4	0.0	0.0	30.9675618301762	0.0
36	2.985702961369482E-4	0.0	0.0	31.676815568649523	0.0
37	4.478554442054223E-4	0.0	0.0	32.38323288930954	0.0
38	4.478554442054223E-4	0.0	0.0	33.06934742983225	0.0
39	4.478554442054223E-4	0.0	0.0	33.72157424174341	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCAA	25	3.8890557E-5	45.000004	42
CGAACCC	20	7.031176E-4	45.000004	14
GATCGCG	20	7.031176E-4	45.000004	23
CGCATAG	20	7.031176E-4	45.000004	24
TCGGGTC	20	7.031176E-4	45.000004	5
CGTCTAT	25	3.8890557E-5	45.000004	18
TAACGTG	20	7.031176E-4	45.000004	17
CACGAAC	25	3.8890557E-5	45.000004	19
TAACGGA	20	7.031176E-4	45.000004	29
TGACGGA	20	7.031176E-4	45.000004	22
CGCTGAT	20	7.031176E-4	45.000004	25
TAGCACG	20	7.031176E-4	45.000004	1
TACGCCC	20	7.031176E-4	45.000004	18
TCGTGAC	20	7.031176E-4	45.000004	37
CGTAGGC	20	7.031176E-4	45.000004	33
TCGTCTA	25	3.8890557E-5	45.000004	17
TTAACGA	25	3.8890557E-5	45.000004	19
TAAGGTG	25	3.8890557E-5	45.000004	25
TGCTACG	20	7.031176E-4	45.000004	1
TAGGCGC	20	7.031176E-4	45.000004	35
>>END_MODULE