##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549713_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	938210
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26737617377773	33.0	31.0	34.0	30.0	34.0
2	32.45659500538259	34.0	31.0	34.0	31.0	34.0
3	32.525734110700164	34.0	31.0	34.0	31.0	34.0
4	35.995604395604396	37.0	35.0	37.0	35.0	37.0
5	35.94870977712879	37.0	35.0	37.0	35.0	37.0
6	35.541994862557424	37.0	35.0	37.0	33.0	37.0
7	35.874843585124864	37.0	35.0	37.0	35.0	37.0
8	35.966371068310934	37.0	35.0	37.0	35.0	37.0
9	37.75639568966436	39.0	38.0	39.0	35.0	39.0
10	37.30195158866352	39.0	37.0	39.0	34.0	39.0
11	37.23617740164782	39.0	37.0	39.0	34.0	39.0
12	37.04287846004626	39.0	37.0	39.0	33.0	39.0
13	36.95054412125217	39.0	37.0	39.0	33.0	39.0
14	38.16394410633014	40.0	37.0	41.0	33.0	41.0
15	38.24108781616056	40.0	37.0	41.0	33.0	41.0
16	38.2502904467017	40.0	37.0	41.0	34.0	41.0
17	38.1891698020699	40.0	37.0	41.0	34.0	41.0
18	38.13558691550932	40.0	37.0	41.0	33.0	41.0
19	38.161600281386896	40.0	37.0	41.0	34.0	41.0
20	38.09888084757144	40.0	37.0	41.0	34.0	41.0
21	37.96235490988158	40.0	36.0	41.0	33.0	41.0
22	38.02784877586041	40.0	37.0	41.0	34.0	41.0
23	37.9755609085386	40.0	36.0	41.0	33.0	41.0
24	37.92572878140288	40.0	36.0	41.0	33.0	41.0
25	37.83502094413831	40.0	36.0	41.0	33.0	41.0
26	37.76275354131804	40.0	36.0	41.0	33.0	41.0
27	37.67756259259654	40.0	36.0	41.0	33.0	41.0
28	37.54872469916117	40.0	36.0	41.0	33.0	41.0
29	37.47258396307863	40.0	36.0	41.0	33.0	41.0
30	37.42520331269119	39.0	36.0	41.0	33.0	41.0
31	37.38401210816342	39.0	35.0	41.0	33.0	41.0
32	37.26483516483516	39.0	35.0	41.0	32.0	41.0
33	37.20858443205679	39.0	35.0	41.0	32.0	41.0
34	37.08320845013377	39.0	35.0	41.0	31.0	41.0
35	37.033502094413834	39.0	35.0	41.0	31.0	41.0
36	36.87161403097387	39.0	35.0	41.0	31.0	41.0
37	36.825857750397034	39.0	35.0	41.0	31.0	41.0
38	36.75577749118001	39.0	35.0	41.0	31.0	41.0
39	36.68164163673378	39.0	35.0	41.0	31.0	41.0
40	36.548538173756405	39.0	35.0	41.0	30.0	41.0
41	36.488203067543516	39.0	35.0	41.0	30.0	41.0
42	36.432859381162	39.0	35.0	41.0	30.0	41.0
43	36.370724038328305	39.0	35.0	41.0	30.0	41.0
44	36.20206563562529	38.0	35.0	40.0	30.0	41.0
45	36.196262030888604	38.0	35.0	40.0	30.0	41.0
46	36.1167009518125	38.0	35.0	40.0	30.0	41.0
47	35.95258843968834	38.0	35.0	40.0	29.0	41.0
48	35.86874900075676	38.0	35.0	40.0	29.0	41.0
49	35.84869698681532	38.0	35.0	40.0	29.0	41.0
50	35.75959433389113	38.0	35.0	40.0	29.0	41.0
51	34.86957291011607	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	17.0
10	32.0
11	31.0
12	28.0
13	21.0
14	27.0
15	49.0
16	63.0
17	107.0
18	206.0
19	377.0
20	650.0
21	1053.0
22	1568.0
23	2309.0
24	3379.0
25	5049.0
26	7096.0
27	8919.0
28	10534.0
29	12305.0
30	14963.0
31	18947.0
32	24705.0
33	34155.0
34	58651.0
35	75418.0
36	76589.0
37	114962.0
38	194331.0
39	271565.0
40	102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.743714093859584	4.899969090075783	52.41619680028992	17.940120015774717
2	20.47633259078458	4.688609159996163	53.52852772833374	21.306530520885516
3	22.01234265249784	4.907749864102919	50.76368830006075	22.316219183338486
4	19.282250242483027	4.773238400784472	50.87134010509374	25.073171251638758
5	18.192515534901567	6.204687649886486	50.03144285394527	25.57135396126667
6	21.78350262734356	7.404099295466899	53.06477227912727	17.747625798062266
7	85.36713528954071	2.494324298398013	8.099679176303813	4.038861235757453
8	86.5573805438015	3.1020773600793	6.600334679869113	3.740207416250093
9	81.67936815851462	5.318851856194242	8.200189722983128	4.801590262308012
10	38.464416282069045	29.588578250071944	16.693384210358023	15.253621257500985
11	28.952473326867118	25.98202960957568	24.723889107982224	20.34160795557498
12	28.200402894874284	22.26218010893084	29.00960339369651	20.527813602498373
13	24.611440935398257	22.516920518860385	30.329457157779178	22.542181387962184
14	20.51726159388623	26.368510248238668	29.334157597979132	23.78007055989597
15	20.22628196246043	26.175909444580636	32.007333113055715	21.59047547990322
16	22.737766598096375	24.920966521354494	31.03260464075207	21.30866223979706
17	22.864603873333262	24.240948188571853	28.4945801046674	24.399867833427486
18	22.972042506475095	25.30584837083382	29.31593140128543	22.40617772140566
19	24.568273627439485	25.99013014143955	27.60277549802283	21.83882073309813
20	26.675264599609893	25.44462327197536	27.913047185598106	19.96706494281664
21	26.043956043956047	25.677513563061577	28.076763198004713	20.20176719497767
22	23.86054294880677	23.794139904712164	28.154784110167235	24.190533036313834
23	23.683397107257438	25.585210134191705	27.562805768431375	23.168586990119483
24	23.873653020112766	24.720584943669326	28.658829046801888	22.746932989416017
25	23.295317679410793	24.92906705321836	27.37457498854201	24.401040278828834
26	21.465876509523454	27.138167361251746	26.905383656111105	24.490572473113694
27	22.781040492000727	27.481907035738267	27.770754948252524	21.966297524008485
28	20.76379488600633	26.78494153760885	30.065230598693255	22.38603297769156
29	22.202598565353174	26.191684164526063	28.271815478411018	23.333901791709746
30	22.6464224427367	26.82896153313224	27.934151202822395	22.59046482130866
31	24.04440370492747	27.732490593790303	25.49280011937626	22.73030558190597
32	23.92641306317349	27.60330842775072	25.280374329840864	23.189904179234926
33	23.222839236418285	27.683247886933625	26.064953475234752	23.028959401413328
34	21.436778546380875	26.909540507988616	27.6361368989885	24.01754404664201
35	23.19864422677226	25.484912759403542	26.45079459822428	24.86564841559992
36	22.753967661824113	28.67268521972693	25.43844128713188	23.13490583131708
37	22.207394932904148	29.298984235938647	26.607902282005096	21.88571854915211
38	22.68234190639622	28.092111574167834	25.99577919655514	23.229767322880804
39	22.439752294262476	26.706067937881713	26.914123703648436	23.940056064207376
40	22.51617441724134	26.221634815233262	28.212873450506816	23.049317317018577
41	20.80440413127125	26.45718975495891	27.814348599993604	24.924057513776233
42	21.139616930111597	27.00088466334829	26.664712590997752	25.194785815542364
43	22.475245414139692	26.159388623016167	26.537448971978556	24.827916990865585
44	22.593129469948092	25.80051374425768	27.541275407424777	24.06508137836945
45	22.53621257500986	26.173671139723513	25.588194540667867	25.701921744598756
46	22.020336598416133	27.138700290979635	26.78995107705098	24.051012033553256
47	21.342236812653884	26.979354302341697	27.592116903465108	24.086291981539315
48	21.2060199742062	26.207672056362647	27.87531576086377	24.71099220856738
49	21.07246778439795	26.18113215591392	28.30954690314535	24.436853156542778
50	20.92228818707965	25.39047761162213	28.151799703691072	25.535434497607145
51	20.569062363436757	26.505366602359814	26.01411197919442	26.911459055009008
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	354.0
1	944.0
2	1534.0
3	2954.0
4	4374.0
5	3137.0
6	1900.0
7	1831.5
8	1763.0
9	1798.0
10	1833.0
11	1845.5
12	1858.0
13	1806.5
14	1755.0
15	1601.5
16	1448.0
17	1858.5
18	2269.0
19	2408.0
20	2547.0
21	2874.0
22	3201.0
23	3485.0
24	3769.0
25	4342.5
26	6658.0
27	8400.0
28	9683.0
29	10966.0
30	13866.0
31	16766.0
32	18537.0
33	20308.0
34	23316.5
35	26325.0
36	29457.0
37	32589.0
38	34641.5
39	36694.0
40	40445.0
41	44196.0
42	47893.5
43	51591.0
44	54677.0
45	57763.0
46	65032.5
47	72302.0
48	80242.5
49	88183.0
50	85512.0
51	82841.0
52	76913.5
53	70986.0
54	62247.5
55	53509.0
56	51652.0
57	49795.0
58	47849.0
59	45903.0
60	42745.0
61	39587.0
62	35412.5
63	31238.0
64	26335.0
65	21432.0
66	18657.0
67	15882.0
68	13029.5
69	10177.0
70	8520.0
71	6863.0
72	5695.0
73	4527.0
74	3620.0
75	2182.0
76	1651.0
77	1216.5
78	782.0
79	653.0
80	524.0
81	340.0
82	156.0
83	89.5
84	23.0
85	16.5
86	10.0
87	7.0
88	4.0
89	2.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	938210.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.924673642729655
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.92684651038024	17.686756761970152
2	10.666638718364082	5.103917004035297
3	4.037965370872429	2.898210110363
4	1.9585196853344626	1.8742777717795436
5	1.0765805172467418	1.2878418762624693
6	0.694353077493394	0.9967302463112655
7	0.5167516832091453	0.8654180762577018
8	0.374622880406524	0.7170184122260342
9	0.3129391256100374	0.6738269805235193
>10	4.219488957489928	26.576624492899377
>50	2.0341320957899747	33.37580520417081
>100	0.1735193345706046	6.074262970979142
>500	0.004944851503307385	0.7188578710321462
>1k	0.0026971917290767554	1.1504522211895372
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2222	0.23683397107257437	No Hit
TCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	1970	0.20997431278711587	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	1875	0.19984864795728036	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	1738	0.18524637341320171	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	1587	0.1691518956310421	Illumina Single End Adapter 1 (95% over 21bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1305	0.13909465897826712	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0658594557721619E-4	0.0	0.0	0.2218053527461869	0.0
2	1.0658594557721619E-4	0.0	0.0	0.8644120186312233	0.0
3	1.0658594557721619E-4	0.0	0.0	1.232666460600505	0.0
4	1.0658594557721619E-4	0.0	0.0	1.630018865712367	0.0
5	1.0658594557721619E-4	0.0	0.0	2.304494729324991	0.0
6	1.0658594557721619E-4	0.0	0.0	2.744801270504471	0.0
7	1.0658594557721619E-4	0.0	0.0	3.127657987017832	0.0
8	1.0658594557721619E-4	0.0	0.0	3.7117489687809764	0.0
9	1.0658594557721619E-4	0.0	0.0	3.982264098655951	0.0
10	1.0658594557721619E-4	0.0	0.0	4.5534581810042525	0.0
11	1.0658594557721619E-4	0.0	0.0	5.536713528954072	0.0
12	1.0658594557721619E-4	0.0	0.0	6.397608211381248	0.0
13	1.0658594557721619E-4	0.0	0.0	6.681766342290105	0.0
14	1.0658594557721619E-4	0.0	0.0	6.7881391159761675	0.0
15	1.0658594557721619E-4	0.0	0.0	6.9700813250764755	0.0
16	1.0658594557721619E-4	0.0	0.0	7.391202396052057	0.0
17	1.0658594557721619E-4	0.0	0.0	7.966979674060179	0.0
18	1.0658594557721619E-4	0.0	0.0	8.653926093305337	0.0
19	1.0658594557721619E-4	0.0	0.0	9.065987358906854	0.0
20	1.0658594557721619E-4	0.0	0.0	9.541467262126815	0.0
21	1.0658594557721619E-4	0.0	0.0	10.178318286950683	0.0
22	1.0658594557721619E-4	0.0	0.0	10.817727374468403	0.0
23	1.0658594557721619E-4	0.0	0.0	11.439656366911459	0.0
24	2.1317189115443237E-4	0.0	0.0	11.929099029002035	0.0
25	2.1317189115443237E-4	0.0	0.0	12.35810745995033	0.0
26	2.1317189115443237E-4	0.0	0.0	12.7605759904499	0.0
27	2.1317189115443237E-4	0.0	0.0	13.147056629112885	0.0
28	2.1317189115443237E-4	0.0	0.0	13.5553873866192	0.0
29	2.1317189115443237E-4	0.0	0.0	13.995054412125217	0.0
30	2.1317189115443237E-4	0.0	0.0	14.460728408352075	0.0
31	2.1317189115443237E-4	0.0	0.0	14.915850395966787	0.0
32	2.1317189115443237E-4	0.0	0.0	15.375875337078053	0.0
33	2.1317189115443237E-4	0.0	0.0	15.836752965753936	0.0
34	2.1317189115443237E-4	0.0	0.0	16.271197279926668	0.0
35	2.1317189115443237E-4	0.0	0.0	16.725466579976764	0.0
36	2.1317189115443237E-4	0.0	0.0	17.15181036228563	0.0
37	2.1317189115443237E-4	0.0	0.0	17.58060562134277	0.0
38	3.197578367316486E-4	0.0	0.0	18.01174577120261	0.0
39	3.197578367316486E-4	0.0	0.0	18.455782820477292	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAATCGC	30	2.165074E-6	45.000004	29
AACGTTA	25	3.8902555E-5	45.0	14
ACCGCGT	20	7.0326234E-4	45.0	26
CGGTTAT	45	3.8380676E-10	45.0	37
CGCATCG	20	7.0326234E-4	45.0	21
CGTTGAT	20	7.0326234E-4	45.0	25
CCTCGTA	25	3.8902555E-5	45.0	34
CGTTAGT	25	3.8902555E-5	45.0	16
AGGTTCG	20	7.0326234E-4	45.0	36
CGTAACG	25	3.8902555E-5	45.0	14
CTTTACG	45	3.8380676E-10	45.0	1
GACGATT	20	7.0326234E-4	45.0	37
CGTCATA	25	3.8902555E-5	45.0	38
TCAAACG	20	7.0326234E-4	45.0	35
CCCTCGT	230	0.0	43.04348	14
ACGAACG	75	0.0	42.0	21
TGGGTAC	505	0.0	41.88119	6
TTCACGG	100	0.0	40.5	2
ACACGCG	95	0.0	40.263157	36
GCGCGAC	280	0.0	40.178574	9
>>END_MODULE