FastQCFastQC Report
Sat 18 Jun 2016
SRR3549712_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549712_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1637946
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT42870.2617302401910686No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGC41620.2540987309715949No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC41360.2525113770539444No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40120.2449409199082265No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCG39220.23944623327020548No Hit
GAATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC35020.213804362292774No Hit
TCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC34800.21246121667014667No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC34060.2079433632122182No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTA351.2123564E-745.00000445
ACTACGC253.8915343E-545.00000445
CACGTAA351.2123564E-745.00000433
ACGCATA207.034167E-445.015
ATATCGA207.034167E-445.041
GGACGTA453.8562575E-1045.08
CATAACG207.034167E-445.019
TATCGAT207.034167E-445.043
CGAACGA950.042.6315822
CGAGGGA2700.040.8333324
CAACCCG451.9292202E-840.023
TTGGGAC52800.039.7159085
TTCGTTG4450.039.43821
CTAGTCG403.4594268E-739.3751
TGTTGCG4750.039.315791
TTTGTCG4200.039.1071431
GCGCGAC4150.039.0361449
TCGCGCG750.039.01
TCGTTCA2950.038.89830416
TTGTTCG2850.038.684211