##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549712_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1637946
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.275455967412846	33.0	31.0	34.0	30.0	34.0
2	32.462626362529655	34.0	31.0	34.0	31.0	34.0
3	32.528962493269006	34.0	31.0	34.0	31.0	34.0
4	36.000453616908004	37.0	35.0	37.0	35.0	37.0
5	35.96770284246245	37.0	35.0	37.0	35.0	37.0
6	35.59696351405968	37.0	35.0	37.0	33.0	37.0
7	35.96084486301746	37.0	35.0	37.0	35.0	37.0
8	36.040200348485236	37.0	35.0	37.0	35.0	37.0
9	37.833887075642295	39.0	38.0	39.0	35.0	39.0
10	37.347015103062006	39.0	37.0	39.0	34.0	39.0
11	37.241940821003865	39.0	37.0	39.0	34.0	39.0
12	37.16372273567016	39.0	37.0	39.0	34.0	39.0
13	37.15598865896678	39.0	37.0	39.0	33.0	39.0
14	38.3907064091246	40.0	38.0	41.0	34.0	41.0
15	38.42227582594298	40.0	38.0	41.0	34.0	41.0
16	38.46317033650682	40.0	38.0	41.0	34.0	41.0
17	38.36711894042905	40.0	38.0	41.0	34.0	41.0
18	38.31891039142926	40.0	38.0	41.0	34.0	41.0
19	38.36306019856576	40.0	37.0	41.0	34.0	41.0
20	38.28969880569933	40.0	37.0	41.0	34.0	41.0
21	38.185544578392694	40.0	37.0	41.0	34.0	41.0
22	38.23483497013943	40.0	37.0	41.0	34.0	41.0
23	38.17423712381238	40.0	37.0	41.0	34.0	41.0
24	38.150507403784985	40.0	37.0	41.0	34.0	41.0
25	38.07076545869034	40.0	37.0	41.0	34.0	41.0
26	38.02909680783127	40.0	37.0	41.0	34.0	41.0
27	37.9678206729648	40.0	37.0	41.0	33.0	41.0
28	37.859359832375425	40.0	37.0	41.0	33.0	41.0
29	37.81702876651611	40.0	37.0	41.0	33.0	41.0
30	37.79602196897822	40.0	37.0	41.0	33.0	41.0
31	37.74674012452181	40.0	37.0	41.0	33.0	41.0
32	37.617338422634205	40.0	36.0	41.0	33.0	41.0
33	37.5644740424898	40.0	36.0	41.0	33.0	41.0
34	37.46079357927551	40.0	36.0	41.0	32.0	41.0
35	37.37521566645054	40.0	36.0	41.0	32.0	41.0
36	37.26130409671625	40.0	36.0	41.0	32.0	41.0
37	37.25511891112406	40.0	36.0	41.0	32.0	41.0
38	37.19149776610462	40.0	36.0	41.0	32.0	41.0
39	37.124168318125264	40.0	36.0	41.0	31.0	41.0
40	37.00772980305822	39.0	35.0	41.0	31.0	41.0
41	36.907192300600876	39.0	35.0	41.0	31.0	41.0
42	36.83621254913166	39.0	35.0	41.0	31.0	41.0
43	36.82662432094831	39.0	35.0	41.0	31.0	41.0
44	36.722426746669306	39.0	35.0	41.0	31.0	41.0
45	36.758260650839524	39.0	35.0	41.0	31.0	41.0
46	36.669962257608006	39.0	35.0	41.0	31.0	41.0
47	36.46509530839234	39.0	35.0	41.0	30.0	41.0
48	36.393846317277855	39.0	35.0	40.0	30.0	41.0
49	36.38525873258337	39.0	35.0	40.0	30.0	41.0
50	36.30508453880653	39.0	35.0	40.0	30.0	41.0
51	35.454255512697	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	21.0
10	30.0
11	18.0
12	29.0
13	26.0
14	34.0
15	32.0
16	63.0
17	122.0
18	254.0
19	430.0
20	796.0
21	1373.0
22	2189.0
23	3336.0
24	4804.0
25	7378.0
26	10268.0
27	13047.0
28	15532.0
29	18646.0
30	23168.0
31	29797.0
32	39598.0
33	54623.0
34	89429.0
35	112033.0
36	129533.0
37	196745.0
38	358098.0
39	526300.0
40	189.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.316707632608153	4.669812069506564	53.68528632812071	16.32819396976457
2	20.30439342933161	5.458604862431362	54.073211204764995	20.163790503472033
3	20.71423600045423	5.697623731185277	52.25355414647369	21.334586121886804
4	18.802695571160466	4.624084066263478	52.789102937459475	23.78411742511658
5	19.213881287905707	5.706048917363575	50.42162562135748	24.658444173373237
6	20.64256086586493	6.950351232580316	54.64056812617755	17.76651977537721
7	84.89193172424487	2.1080060026398915	9.06904134812747	3.931020924987759
8	86.78069973002773	3.3631755869851636	6.195686548885006	3.660438134102101
9	81.08405283202255	5.6276580546611426	8.091719751444797	5.196569361871515
10	35.3619716400907	37.331084174936166	14.791940637847645	12.515003547125486
11	23.893705897508223	25.63521630139211	31.318431743171022	19.15264605792865
12	23.4004661936352	22.753131055602566	33.640669472619976	20.20573327814226
13	23.425436491801317	22.720651352364484	34.27548893553267	19.578423220301524
14	18.89659366059687	26.525233432604008	32.449482461570774	22.12869044522835
15	17.577929919545575	26.845817871895655	35.32204358385441	20.254208624704358
16	20.95417065031448	26.038465248549098	32.27279776012152	20.734566341014904
17	20.280766276788125	25.75988463600143	31.147363832507295	22.811985254703146
18	20.010183485902466	26.05671981860208	32.416697497963916	21.516399197531545
19	22.316975040691204	26.79892987925121	30.741794906547593	20.142300173509994
20	24.19731786029576	26.52517238053025	31.816433508796994	17.461076250376998
21	24.919197580384214	26.454046714604758	30.271754990701773	18.355000714309263
22	22.460020049501022	24.013734274512103	31.063417231093087	22.462828444893788
23	21.25503526978301	26.099151009862354	30.777510369694728	21.86830335065991
24	20.970044189490984	25.29546151094114	31.481318675951464	22.253175623616407
25	20.558858472745744	27.160907624549285	29.74786714580334	22.53236675690163
26	19.538861476507773	28.097324331815578	29.981269223771722	22.382544967904924
27	19.7432027673684	27.561531332534773	31.544690728509977	21.15057517158685
28	18.005721800352394	26.630914572275277	33.92553844876449	21.43782517860784
29	19.13335360262182	24.379802508751816	33.31520086742786	23.171643021198502
30	20.438646939520595	25.474832503635653	32.50461248417225	21.581908072671503
31	21.07090221533555	26.480115950098476	29.89958154908648	22.549400285479496
32	20.466791945522015	28.162283738291737	29.277766177883763	22.093158138302485
33	20.247248688296196	26.948934824469184	29.325020482970743	23.478796004263877
34	20.618567400878906	25.733876452581466	30.384762379223734	23.262793767315895
35	19.609315569621955	25.932662004730318	30.623292831387605	23.83472959426013
36	19.679891766883646	26.83562217557844	31.617770060795653	21.86671599674226
37	19.527383686641684	27.735896055181307	31.34492834318103	21.391791914995977
38	19.01314206939667	27.774969382385013	29.62832718538951	23.58356136282881
39	20.513374677797678	25.514333195355647	28.900159101704208	25.072133025142463
40	20.982193552168386	25.58258941381462	30.41632630135548	23.018890732661514
41	19.60705664289299	27.01902260514083	30.48384989492938	22.8900708570368
42	19.52787210323173	27.87778107458976	30.278226510519886	22.316120311658626
43	20.237846668937802	26.2831619601623	30.06991683486513	23.409074536034765
44	19.979474292803303	24.86882961953569	30.83434985036137	24.317346237299642
45	20.532850289325776	24.076068441816766	29.69200449831679	25.699076770540664
46	21.19514318542858	25.048322716377708	30.139149886504196	23.61738421168952
47	18.333937748863516	26.03944208172919	32.75053023726057	22.876089932146726
48	18.89122107810636	25.288745782828002	32.02572001763183	23.794313121433795
49	19.170961680055388	25.030373406693506	32.32682884539539	23.471836067855715
50	19.979596396950818	23.744006212659023	30.761087361854422	25.515310028535737
51	18.677172507518563	24.398972859911133	28.916948421987048	28.006906210583253
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	396.0
1	1616.5
2	2837.0
3	5275.0
4	7713.0
5	5460.5
6	3208.0
7	3105.0
8	3002.0
9	3115.5
10	3229.0
11	3183.0
12	3137.0
13	3177.0
14	3217.0
15	3385.0
16	3553.0
17	3645.5
18	3738.0
19	4641.0
20	5544.0
21	6204.0
22	6864.0
23	8843.0
24	10822.0
25	13313.5
26	20792.0
27	25779.0
28	29378.5
29	32978.0
30	37947.0
31	42916.0
32	48065.0
33	53214.0
34	57075.0
35	60936.0
36	65017.0
37	69098.0
38	75391.5
39	81685.0
40	89835.0
41	97985.0
42	105424.5
43	112864.0
44	132910.0
45	152956.0
46	156574.5
47	160193.0
48	153915.0
49	147637.0
50	129510.5
51	111384.0
52	99937.0
53	88490.0
54	78478.5
55	68467.0
56	64955.5
57	61444.0
58	58192.5
59	54941.0
60	50844.5
61	46748.0
62	40289.5
63	33831.0
64	28449.0
65	23067.0
66	18558.5
67	14050.0
68	11881.5
69	9713.0
70	7778.0
71	5843.0
72	4832.0
73	3821.0
74	3286.0
75	1973.5
76	1196.0
77	809.0
78	422.0
79	344.5
80	267.0
81	176.5
82	86.0
83	94.0
84	102.0
85	55.5
86	9.0
87	6.0
88	3.0
89	2.5
90	2.0
91	1.0
92	0.0
93	1.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1637946.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.4054213508709
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.93607138569433	16.368832184067617
2	11.958175767420396	5.597722848484968
3	5.011440022362139	3.5188459589401124
4	2.5382492950547944	2.37635176977234
5	1.5478152037129145	1.811363350809242
6	0.9711366289633783	1.363791719409135
7	0.6825394272971034	1.1182586019129575
8	0.497432455081623	0.9314092963826839
9	0.37742744098745035	0.7950463457123013
>10	4.448427545260635	26.62144104653058
>50	1.846749187497105	29.621610961708445
>100	0.17262157500617867	6.235479203835061
>500	0.00847222447072493	1.3500858102729585
>1k	0.003441841191232003	2.28976090216166
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT	4287	0.2617302401910686	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGC	4162	0.2540987309715949	No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	4136	0.2525113770539444	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4012	0.2449409199082265	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCG	3922	0.23944623327020548	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC	3502	0.213804362292774	No Hit
TCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	3480	0.21246121667014667	No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	3406	0.2079433632122182	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.105207375578926E-5	0.0	0.0	0.2787637687689338	0.0
2	6.105207375578926E-5	0.0	0.0	1.0250032662859458	0.0
3	6.105207375578926E-5	0.0	0.0	1.4399742116040455	0.0
4	6.105207375578926E-5	0.0	0.0	2.0255246509958202	0.0
5	6.105207375578926E-5	0.0	0.0	3.054740510370916	0.0
6	6.105207375578926E-5	0.0	0.0	3.586565124857596	0.0
7	6.105207375578926E-5	0.0	0.0	4.105629855929316	0.0
8	6.105207375578926E-5	0.0	0.0	4.8820901299554444	0.0
9	6.105207375578926E-5	0.0	0.0	5.24376261488474	0.0
10	6.105207375578926E-5	0.0	0.0	6.247580811577427	0.0
11	1.2210414751157852E-4	0.0	0.0	7.648542748051523	0.0
12	1.2210414751157852E-4	0.0	0.0	9.291148792451033	0.0
13	1.2210414751157852E-4	0.0	0.0	9.699404009655996	0.0
14	1.2210414751157852E-4	0.0	0.0	9.849470006947726	0.0
15	1.2210414751157852E-4	0.0	0.0	10.098257207502567	0.0
16	1.2210414751157852E-4	0.0	0.0	10.621107167147146	0.0
17	1.2210414751157852E-4	0.0	0.0	11.411792574358373	0.0
18	1.2210414751157852E-4	0.0	0.0	12.291003488515495	0.0
19	1.2210414751157852E-4	0.0	0.0	12.965506799369454	0.0
20	1.2210414751157852E-4	0.0	0.0	13.615528228647342	0.0
21	1.2210414751157852E-4	0.0	0.0	14.471966719293555	0.0
22	1.2210414751157852E-4	0.0	0.0	15.45826297081833	0.0
23	1.2210414751157852E-4	0.0	0.0	16.345227498342435	0.0
24	1.2210414751157852E-4	0.0	0.0	17.002025707807217	0.0
25	1.8315622126736778E-4	0.0	0.0	17.584340387289934	0.0
26	1.8315622126736778E-4	0.0	0.0	18.100963035411425	0.0
27	1.8315622126736778E-4	0.0	0.0	18.59920900933242	0.0
28	1.8315622126736778E-4	0.0	0.0	19.142511413685188	0.0
29	1.8315622126736778E-4	0.0	0.0	19.751811109767967	0.0
30	2.4420829502315704E-4	0.0	0.0	20.399634664390646	0.0
31	2.4420829502315704E-4	0.0	0.0	21.013513266005106	0.0
32	2.4420829502315704E-4	0.0	0.0	21.58502172843305	0.0
33	2.4420829502315704E-4	0.0	0.0	22.171854261373696	0.0
34	2.4420829502315704E-4	0.0	0.0	22.7381732975324	0.0
35	2.4420829502315704E-4	0.0	0.0	23.34496985859119	0.0
36	2.4420829502315704E-4	0.0	0.0	23.9082362910621	0.0
37	2.4420829502315704E-4	0.0	0.0	24.510576050736717	0.0
38	2.4420829502315704E-4	0.0	0.0	25.06944673389721	0.0
39	2.4420829502315704E-4	0.0	0.0	25.652066673748706	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTA	35	1.2123564E-7	45.000004	45
ACTACGC	25	3.8915343E-5	45.000004	45
CACGTAA	35	1.2123564E-7	45.000004	33
ACGCATA	20	7.034167E-4	45.0	15
ATATCGA	20	7.034167E-4	45.0	41
GGACGTA	45	3.8562575E-10	45.0	8
CATAACG	20	7.034167E-4	45.0	19
TATCGAT	20	7.034167E-4	45.0	43
CGAACGA	95	0.0	42.63158	22
CGAGGGA	270	0.0	40.833332	4
CAACCCG	45	1.9292202E-8	40.0	23
TTGGGAC	5280	0.0	39.715908	5
TTCGTTG	445	0.0	39.4382	1
CTAGTCG	40	3.4594268E-7	39.375	1
TGTTGCG	475	0.0	39.31579	1
TTTGTCG	420	0.0	39.107143	1
GCGCGAC	415	0.0	39.036144	9
TCGCGCG	75	0.0	39.0	1
TCGTTCA	295	0.0	38.898304	16
TTGTTCG	285	0.0	38.68421	1
>>END_MODULE