FastQCFastQC Report
Sat 18 Jun 2016
SRR3549711_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549711_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2035096
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195880.96250987668395No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT47110.23148785118736415No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC46670.22932579101919517No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC31860.15655281126787138No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC26700.13119774202298073No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCC25440.12500638790504232No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACG207.034569E-445.00000440
GGTACGA253.8918693E-545.09
CGTTTTT119600.043.7395481
CGCGGAT950.042.631584
TATTTCG850.039.7058831
CGTTCAT2900.039.5689717
TCGTTCA2850.039.47368216
TCTTGCG5700.038.6842081
TACGAAT2850.038.68420812
GCTACGA2800.038.57142610
TACGGGA3950.038.164564
TGGGCGA25600.037.8808636
TTGGGAC56550.037.6790475
TTTTGCG17500.037.671431
CGTTTGG11950.037.6569062
GGGCGAT37900.037.6385237
TTTGGGA138500.037.6245464
TTGGGAT83150.037.6127475
TATATCG301.1402312E-437.49999645
TACGATT301.1402312E-437.49999611