##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549711_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2035096
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.097215069952476	33.0	31.0	34.0	30.0	34.0
2	32.31480087425851	34.0	31.0	34.0	30.0	34.0
3	32.327114298293544	34.0	31.0	34.0	30.0	34.0
4	35.87964155007921	37.0	35.0	37.0	35.0	37.0
5	35.870517164792226	37.0	35.0	37.0	35.0	37.0
6	35.53192871491075	37.0	35.0	37.0	33.0	37.0
7	35.89055553153266	37.0	35.0	37.0	35.0	37.0
8	35.97632494978124	37.0	35.0	37.0	35.0	37.0
9	37.7481371886142	39.0	38.0	39.0	35.0	39.0
10	37.188777335319806	39.0	37.0	39.0	34.0	39.0
11	37.07795553624989	39.0	37.0	39.0	33.0	39.0
12	36.99005550598105	39.0	37.0	39.0	33.0	39.0
13	36.98033753690244	39.0	37.0	39.0	33.0	39.0
14	38.16577104962125	40.0	37.0	41.0	33.0	41.0
15	38.1720346362039	40.0	37.0	41.0	33.0	41.0
16	38.17896453042019	40.0	37.0	41.0	33.0	41.0
17	38.09686226104321	40.0	37.0	41.0	33.0	41.0
18	38.008509180893675	39.0	37.0	41.0	33.0	41.0
19	37.95840933302409	40.0	37.0	41.0	33.0	41.0
20	37.83766810017808	39.0	36.0	41.0	33.0	41.0
21	37.66885296811551	39.0	36.0	41.0	33.0	41.0
22	37.74866738473271	39.0	36.0	41.0	33.0	41.0
23	37.686110630653296	39.0	36.0	41.0	33.0	41.0
24	37.61970983187034	39.0	36.0	41.0	33.0	41.0
25	37.535439605797464	39.0	35.0	41.0	33.0	41.0
26	37.480652509758755	39.0	35.0	41.0	33.0	41.0
27	37.4169361052255	39.0	35.0	41.0	33.0	41.0
28	37.31319554458365	39.0	35.0	41.0	32.0	41.0
29	37.21118168381246	39.0	35.0	41.0	32.0	41.0
30	37.13276327013566	39.0	35.0	41.0	32.0	41.0
31	37.00357378718252	39.0	35.0	41.0	31.0	41.0
32	36.75732741846085	39.0	35.0	41.0	31.0	41.0
33	36.55203243483354	39.0	35.0	41.0	30.0	41.0
34	36.29875052577372	39.0	35.0	41.0	30.0	41.0
35	36.159821453140296	39.0	35.0	41.0	29.0	41.0
36	35.99512799396196	39.0	35.0	41.0	29.0	41.0
37	35.97873220722757	39.0	35.0	41.0	29.0	41.0
38	35.90139875465334	39.0	35.0	41.0	28.0	41.0
39	35.78397923242933	39.0	35.0	41.0	27.0	41.0
40	35.64940425414821	39.0	35.0	40.0	27.0	41.0
41	35.53371536281335	38.0	35.0	40.0	26.0	41.0
42	35.495568268032564	38.0	35.0	40.0	26.0	41.0
43	35.48384990192109	38.0	35.0	40.0	26.0	41.0
44	35.35904153907236	38.0	35.0	40.0	26.0	41.0
45	35.38095500163137	38.0	35.0	40.0	26.0	41.0
46	35.250929685872315	38.0	34.0	40.0	25.0	41.0
47	35.02937502702575	38.0	34.0	40.0	24.0	41.0
48	34.9358148215121	38.0	34.0	40.0	24.0	41.0
49	34.92031432423827	38.0	34.0	40.0	24.0	41.0
50	34.80691230290856	38.0	34.0	40.0	24.0	41.0
51	33.991444138261784	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	15.0
10	26.0
11	36.0
12	28.0
13	26.0
14	38.0
15	62.0
16	126.0
17	271.0
18	545.0
19	1052.0
20	1929.0
21	3176.0
22	5169.0
23	8067.0
24	13084.0
25	20987.0
26	28829.0
27	31560.0
28	31405.0
29	32999.0
30	37841.0
31	45438.0
32	57865.0
33	76971.0
34	119618.0
35	149099.0
36	171255.0
37	253217.0
38	430769.0
39	513434.0
40	154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.034537928431877	4.731029887533561	55.94645166616219	17.287980517872377
2	24.374378407701652	4.192431217004013	50.68719116936007	20.745999205934265
3	19.333928227464455	4.044280957753344	54.70567481828867	21.91611599649353
4	17.129069095511955	4.521015224834602	54.46332752852937	23.886588151124073
5	16.98563605844638	5.218721868648949	54.27183779045313	23.52380428245154
6	20.104751815147786	6.000552308097505	56.3234854768522	17.57121039990251
7	80.94836803767488	1.7075852932736342	12.930102560272342	4.413944108779144
8	83.3842236435038	2.175769595144406	10.650357526131446	3.7896492352203532
9	78.05504015535385	4.928416153341169	12.609478864879101	4.407064826425879
10	35.229050619725065	32.36746571169124	19.34960316368368	13.053880504900015
11	23.338161934375577	25.791805398860788	31.86680136956684	19.00323129719679
12	22.645123375015235	21.99326223431229	35.363344038807014	19.998270351865465
13	22.710722246026723	22.00736476313648	35.66627815100614	19.61563483983065
14	19.378889251416147	24.92634254108897	34.44058658657872	21.254181620916164
15	17.466743583595072	26.60665639360502	35.58623278705279	20.34036723574711
16	20.646298749543018	25.793918321297866	33.817372743104016	19.742410186055103
17	20.55740859399262	25.850131885670258	32.26324458403928	21.329214936297845
18	21.33049251730631	24.555794910903465	33.08979035878406	21.02392221300617
19	21.153400134440833	27.389273036751092	31.76995090157909	19.687375927228988
20	22.91852571082642	26.955829110764306	31.46785213080857	18.657793047600705
21	22.58905722383612	27.184417835817083	32.30781250614221	17.91871243420458
22	21.464048870421838	24.703109828725527	32.363239866817096	21.469601434035543
23	20.450190064743875	25.72424101860551	31.778648279982864	22.046920636667757
24	21.169861274357572	25.219399969338056	32.22924127412171	21.38149748218266
25	20.268380459693304	26.99130655261472	29.938636801408876	22.801676186283103
26	19.85822781824543	27.845369456772556	29.970871153007035	22.32553157197498
27	19.458738064445118	26.51688175889491	31.952399297133894	22.071980879526077
28	18.504041087005234	25.898483413067492	32.55674425186822	23.04073124805906
29	19.33741700637218	25.08967635924792	31.761204385444223	23.811702248935678
30	20.92515537350572	25.33064779253657	31.506916627028897	22.237280206928816
31	21.01021278603073	27.579042954239014	28.973031247665958	22.437713012064293
32	19.954881735308803	27.781293855425005	29.546861671390438	22.716962737875757
33	21.027312716451704	26.92777146630921	29.66675773526163	22.37815808197746
34	18.570819263562996	27.50749841776506	29.852400083337592	24.069282235334352
35	18.510134165660983	27.478851120536817	29.173021813221588	24.83799290058061
36	19.94692142287145	28.78940354656488	28.166975906787687	23.09669912377598
37	19.800589259671288	28.263973787968727	29.75894011879538	22.176496833564606
38	18.75695298894991	28.8163310232048	30.20830466965686	22.21841131818843
39	18.841764712819444	28.420428323774406	30.523424939167487	22.21438202423866
40	20.360808531882526	28.67103075235763	29.32888669625413	21.639274019505713
41	18.564382220789586	28.835543876062847	29.320533281968025	23.279540621179542
42	19.57455569663544	27.714171714749575	29.443819849284754	23.26745273933023
43	20.066571798087164	25.337035697578887	30.84173916119928	23.754653343134674
44	20.324397473141318	25.609946656079124	29.030964632626667	25.034691238152895
45	20.861227185351453	25.082993627819032	28.04344365081549	26.012335536014024
46	20.663103853577425	26.7051284067189	28.284513359566333	24.34725438013735
47	18.53091942591406	27.513738909614094	30.17091085629376	23.784430808178094
48	18.535341821712585	26.597467637890297	30.870189907503136	23.99700063289398
49	19.948837794384147	25.559777032631388	30.66037179572855	23.831013377255914
50	18.940777240975365	25.751463321632002	30.15042042242725	25.157339014965384
51	17.735232146296784	25.778292522809735	29.08953680809161	27.39693852280187
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	493.0
1	1272.5
2	2052.0
3	15840.0
4	29628.0
5	19878.5
6	10129.0
7	10338.5
8	10548.0
9	11026.5
10	11505.0
11	11745.0
12	11985.0
13	12056.0
14	12127.0
15	11792.0
16	11457.0
17	11394.5
18	11332.0
19	10975.5
20	10619.0
21	10667.0
22	10715.0
23	11504.0
24	12293.0
25	14005.0
26	17900.5
27	20084.0
28	24720.0
29	29356.0
30	31843.5
31	34331.0
32	40342.5
33	46354.0
34	53297.5
35	60241.0
36	64724.5
37	69208.0
38	78638.0
39	88068.0
40	99575.0
41	111082.0
42	125333.5
43	139585.0
44	159786.0
45	179987.0
46	198795.0
47	217603.0
48	208283.5
49	198964.0
50	178374.0
51	157784.0
52	139405.5
53	121027.0
54	106927.0
55	92827.0
56	86401.5
57	79976.0
58	72296.5
59	64617.0
60	59175.5
61	53734.0
62	47004.0
63	40274.0
64	34681.0
65	29088.0
66	22296.5
67	15505.0
68	12519.0
69	9533.0
70	7654.0
71	5775.0
72	4762.0
73	3749.0
74	3310.5
75	2139.5
76	1407.0
77	1048.5
78	690.0
79	530.5
80	371.0
81	315.5
82	260.0
83	178.0
84	96.0
85	61.5
86	27.0
87	18.5
88	10.0
89	9.0
90	8.0
91	4.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2035096.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.596044970991898
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.78025390359937	23.311642203093264
2	11.040222619922337	6.976547407776564
3	4.34861727417493	4.121973208693898
4	2.1322497221393832	2.694826324404038
5	1.3536730434173727	2.1385357177917386
6	0.8835365213197138	1.6749755796678854
7	0.6137493476404817	1.3574436392275921
8	0.4847855996315633	1.2253846085798519
9	0.38865878418032723	1.105207238099939
>10	4.083286508914803	30.196710613864603
>50	0.7937350057602328	16.473963681209717
>100	0.08982516045130444	5.125509798391602
>500	0.004727558839235434	1.01866835198814
>1k	0.0025213647142588985	1.6033109928329883
>5k	0.0	0.0
>10k+	1.5758529464118115E-4	0.9753006343782048
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19588	0.96250987668395	No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	4711	0.23148785118736415	No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	4667	0.22932579101919517	No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	3186	0.15655281126787138	No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC	2670	0.13119774202298073	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCC	2544	0.12500638790504232	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4741319328424803E-4	0.0	0.0	0.25114294362526385	0.0
2	1.4741319328424803E-4	0.0	0.0	0.8346535003754123	0.0
3	1.4741319328424803E-4	0.0	0.0	1.2946318011533609	0.0
4	1.4741319328424803E-4	0.0	0.0	1.9367636710995453	0.0
5	1.4741319328424803E-4	0.0	0.0	2.953030225601151	0.0
6	1.4741319328424803E-4	0.0	0.0	3.9981897659864694	0.0
7	1.4741319328424803E-4	0.0	0.0	4.7802658940905	0.0
8	1.9655092437899734E-4	0.0	0.0	5.880656244226317	0.0
9	2.456886554737467E-4	0.0	0.0	6.519938125769006	0.0
10	2.456886554737467E-4	0.0	0.0	7.49522381253759	0.0
11	2.456886554737467E-4	0.0	0.0	9.354693832625095	0.0
12	2.456886554737467E-4	0.0	0.0	10.937076187069307	0.0
13	2.456886554737467E-4	0.0	0.0	11.508449724239053	0.0
14	2.456886554737467E-4	0.0	0.0	11.717334219122833	0.0
15	2.456886554737467E-4	0.0	0.0	11.959534095688852	0.0
16	2.456886554737467E-4	0.0	0.0	12.499508622689053	0.0
17	2.456886554737467E-4	0.0	0.0	13.331361272392064	0.0
18	2.456886554737467E-4	0.0	0.0	14.246797202687244	0.0
19	2.456886554737467E-4	0.0	0.0	14.937034911375187	0.0
20	2.456886554737467E-4	0.0	0.0	15.637444130399745	0.0
21	2.456886554737467E-4	0.0	0.0	16.641721078514234	0.0
22	2.456886554737467E-4	0.0	0.0	17.71877100638004	0.0
23	2.456886554737467E-4	0.0	0.0	18.770613278194247	0.0
24	2.456886554737467E-4	0.0	0.0	19.52654813335587	0.0
25	2.456886554737467E-4	0.0	0.0	20.192855767000673	0.0
26	2.456886554737467E-4	0.0	0.0	20.789043858373265	0.0
27	2.9482638656849606E-4	0.0	0.0	21.40159481420041	0.0
28	3.4396411766324535E-4	0.0	0.0	22.05537232641605	0.0
29	3.4396411766324535E-4	0.0	0.0	22.749934155440332	0.0
30	3.931018487579947E-4	0.0	0.0	23.542280069343168	0.0
31	4.4223957985274403E-4	0.0	0.0	24.244163420300566	0.0
32	4.4223957985274403E-4	0.0	0.0	24.913124491424483	0.0
33	4.4223957985274403E-4	0.0	0.0	25.578203681791916	0.0
34	4.4223957985274403E-4	0.0	0.0	26.243135458966062	0.0
35	4.4223957985274403E-4	0.0	0.0	26.953617912865045	0.0
36	4.4223957985274403E-4	0.0	0.0	27.60031959180304	0.0
37	4.4223957985274403E-4	0.0	0.0	28.261762590069463	0.0
38	4.4223957985274403E-4	0.0	0.0	28.963449390102483	0.0
39	4.4223957985274403E-4	0.0	0.0	29.671868059295484	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAACG	20	7.034569E-4	45.000004	40
GGTACGA	25	3.8918693E-5	45.0	9
CGTTTTT	11960	0.0	43.739548	1
CGCGGAT	95	0.0	42.63158	4
TATTTCG	85	0.0	39.705883	1
CGTTCAT	290	0.0	39.56897	17
TCGTTCA	285	0.0	39.473682	16
TCTTGCG	570	0.0	38.684208	1
TACGAAT	285	0.0	38.684208	12
GCTACGA	280	0.0	38.571426	10
TACGGGA	395	0.0	38.16456	4
TGGGCGA	2560	0.0	37.880863	6
TTGGGAC	5655	0.0	37.679047	5
TTTTGCG	1750	0.0	37.67143	1
CGTTTGG	1195	0.0	37.656906	2
GGGCGAT	3790	0.0	37.638523	7
TTTGGGA	13850	0.0	37.624546	4
TTGGGAT	8315	0.0	37.612747	5
TATATCG	30	1.1402312E-4	37.499996	45
TACGATT	30	1.1402312E-4	37.499996	11
>>END_MODULE