FastQCFastQC Report
Sat 18 Jun 2016
SRR3549709_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549709_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658446
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGC114631.7409172506173627No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCG109501.6630065335653947No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTC100921.5326997202504078No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC34120.5181897984041212No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT28750.4366341355251608Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCGTTGAT28130.4272180254720964No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC27090.411423260221795No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCT24860.3775556385793216No Hit
GAACTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT22950.3485479447061718No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTC22610.34338427145126554No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCGTTGATCGTA19970.30328986735434643No Hit
TCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC18710.28415390176263505No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17320.263043590514636No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGC9120.13850794142572057No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCG8250.12529501280287222No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTCGTTGATCGT6930.10524781075441265No Hit
TCCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG6860.10418470155487314No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCATC207.031088E-445.00000440
GGTGTAC406.8066583E-945.0000048
CAACCCG207.031088E-445.00000423
ATATGGA207.031088E-445.00000429
CGTTACG207.031088E-445.00000445
GGAACGT351.2108649E-745.0000048
ACTCGTC207.031088E-445.0000041
GCGATAT207.031088E-445.0000049
ACGCTAG207.031088E-445.00000437
ACGTCAT207.031088E-445.00000411
CATCGCA207.031088E-445.00000443
TTACGGT207.031088E-445.00000429
TTACGGG406.8066583E-945.0000043
CGATCGC351.2108649E-745.00000434
TATCGAA207.031088E-445.00000435
CAATAGT207.031088E-445.00000419
GGCTCGT207.031088E-445.0000048
GGTAGGC207.031088E-445.0000048
CTTACGA207.031088E-445.00000429
TATCCGG207.031088E-445.0000042