##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549709_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	658446
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98722446487639	33.0	31.0	34.0	30.0	34.0
2	32.16620649225601	33.0	31.0	34.0	30.0	34.0
3	32.24866276049972	34.0	31.0	34.0	30.0	34.0
4	35.8273465098125	37.0	35.0	37.0	35.0	37.0
5	35.76791870555824	37.0	35.0	37.0	35.0	37.0
6	35.14241410837032	37.0	35.0	37.0	32.0	37.0
7	35.67394592722866	37.0	35.0	37.0	35.0	37.0
8	35.61772871275701	37.0	35.0	37.0	35.0	37.0
9	37.47307448143052	39.0	37.0	39.0	35.0	39.0
10	37.08600249678789	39.0	37.0	39.0	33.0	39.0
11	37.025917083557346	39.0	37.0	39.0	33.0	39.0
12	36.77204053179759	39.0	35.0	39.0	33.0	39.0
13	36.58044699185658	39.0	35.0	39.0	32.0	39.0
14	37.641211276247404	40.0	36.0	41.0	33.0	41.0
15	37.65366939733858	40.0	36.0	41.0	33.0	41.0
16	37.81055697809691	40.0	36.0	41.0	33.0	41.0
17	37.633795330216905	39.0	36.0	41.0	32.0	41.0
18	37.61751912837195	39.0	36.0	41.0	33.0	41.0
19	37.67371508065961	39.0	36.0	41.0	33.0	41.0
20	37.44324971220115	39.0	35.0	41.0	32.0	41.0
21	37.38940323124447	39.0	35.0	41.0	32.0	41.0
22	37.489578492389654	39.0	35.0	41.0	33.0	41.0
23	37.36765961065904	39.0	35.0	41.0	32.0	41.0
24	37.34021772476407	39.0	35.0	41.0	32.0	41.0
25	37.297597676954524	39.0	35.0	41.0	32.0	41.0
26	37.23145254128661	39.0	35.0	41.0	32.0	41.0
27	37.06167096466529	39.0	35.0	41.0	32.0	41.0
28	36.93226475671506	39.0	35.0	41.0	31.0	41.0
29	36.8731528477658	39.0	35.0	41.0	31.0	41.0
30	36.75469210838854	39.0	35.0	40.0	31.0	41.0
31	36.815128347654934	39.0	35.0	40.0	31.0	41.0
32	36.570645125036826	39.0	35.0	40.0	30.0	41.0
33	36.4327568244017	39.0	35.0	40.0	30.0	41.0
34	36.33269850526847	39.0	35.0	40.0	30.0	41.0
35	36.17852033424153	39.0	35.0	40.0	30.0	41.0
36	36.05161547036507	38.0	35.0	40.0	30.0	41.0
37	35.91048013048906	38.0	35.0	40.0	29.0	41.0
38	35.87430556188359	38.0	35.0	40.0	29.0	41.0
39	35.85008489686322	38.0	35.0	40.0	29.0	41.0
40	35.591518514806076	38.0	35.0	40.0	27.0	41.0
41	35.5057726829535	38.0	34.0	40.0	27.0	41.0
42	35.554625588127195	38.0	34.0	40.0	28.0	41.0
43	35.42663939032206	38.0	34.0	40.0	27.0	41.0
44	35.35949341327915	38.0	34.0	40.0	27.0	41.0
45	35.42568714822476	38.0	34.0	40.0	27.0	41.0
46	35.26420997317928	38.0	34.0	40.0	26.0	41.0
47	34.887598983060116	37.0	34.0	40.0	26.0	41.0
48	34.871895341455485	37.0	34.0	40.0	26.0	41.0
49	34.90200866889616	37.0	34.0	40.0	26.0	41.0
50	34.743084474656996	37.0	34.0	40.0	26.0	41.0
51	33.759266515401414	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	14.0
10	19.0
11	23.0
12	12.0
13	10.0
14	18.0
15	25.0
16	47.0
17	87.0
18	178.0
19	367.0
20	807.0
21	1486.0
22	2256.0
23	2963.0
24	3965.0
25	5120.0
26	6597.0
27	7908.0
28	9270.0
29	11018.0
30	13973.0
31	17562.0
32	22407.0
33	29973.0
34	49374.0
35	59211.0
36	57605.0
37	81734.0
38	130178.0
39	144193.0
40	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.874899384307902	4.3856291935861105	46.98790789221895	16.751563529887036
2	19.310163627693083	14.184154812999092	46.32331276976396	20.182368789543865
3	20.21745139312867	14.400269725991196	45.7689772585755	19.61330162230464
4	18.611245265367245	4.881341826057111	53.78755433247373	22.719858576101913
5	23.656457780896233	5.960397663589725	45.25321742405603	25.129927131458007
6	19.999362134480275	13.63133802923854	49.500642421702004	16.868657414579175
7	75.31080756812251	2.3470413670976815	17.250161744471075	5.091989320308727
8	74.47398875534212	9.796399401013902	9.86762771738305	5.861984126260923
9	69.28495275238971	5.610027245970057	11.425842058422408	13.679177943217818
10	40.00206546930196	25.823985566014525	18.748386352107843	15.425562612575671
11	31.89373160441403	21.880306053951273	26.148841362845243	20.077120978789452
12	26.622228702125916	19.17909745066414	31.077263739167677	23.12141010804227
13	25.703246735495394	20.862606804506367	33.893136263262285	19.54101019673595
14	20.50084593117735	27.79286380356172	29.46179337409598	22.244496891164957
15	16.470143337494648	23.093921141597033	39.22007271666924	21.215862804239073
16	19.52597479519961	22.90028339453805	31.556574115417206	26.017167694845135
17	19.635323170009386	23.555158661454396	31.792128739486607	25.017389429049615
18	20.20302348256349	23.2720678688913	31.778308319892595	24.746600328652615
19	21.99375499281642	24.996582863287195	28.670080765924617	24.33958137797177
20	25.10213441952719	24.999316572657442	31.75780549961576	18.14074350819961
21	24.01776303599688	28.211425082694706	28.842304456250016	18.928507425058395
22	19.899277996980768	21.954419952433458	31.775878356007937	26.370423694577838
23	21.482855086066284	26.44104451997582	29.85134695935582	22.22475343460208
24	23.327501420010144	23.22863226445297	27.884443067464908	25.559423248071976
25	20.419290268298386	28.791730832900498	26.034329314780557	24.75464958402056
26	20.59819635930661	23.26462610449452	30.396266360491218	25.740911175707655
27	21.468275302758315	26.325317489968807	28.404455338782526	23.801951868490352
28	17.813002129255853	25.055661360233035	32.48922462889895	24.64211188161216
29	24.11192413652752	21.79176424490391	30.31152137001364	23.78479024855493
30	21.344802762868937	23.961873866649658	33.5764815945423	21.116841775939104
31	24.377093945441235	23.128396254210674	26.514247182001256	25.980262618346835
32	28.394431737758296	25.593290869714448	25.606048180108925	20.40622921241833
33	22.624330620886145	23.194916515553288	28.254405068904664	25.926347794655904
34	24.763306330359665	22.76390167151141	29.071328552379388	23.40146344574954
35	24.015333072112217	22.590766744729258	29.103829319336743	24.290070863821786
36	23.366836460393106	26.31772385282924	27.430343566518744	22.885096120258915
37	22.79533932926922	24.31148492055537	32.34509739598995	20.548078354185463
38	21.724029001618963	25.153771152076253	27.05248418245384	26.069715663850946
39	25.868788025137977	23.336613784577626	29.765083241450323	21.02951494883407
40	23.74651831737151	21.58415420550812	32.234989657466215	22.434337819654154
41	20.3012851471495	25.28514107459078	30.055615798410194	24.357957979849523
42	24.853214994092152	22.034456887884506	28.884525078746016	24.22780303927733
43	23.723889278695594	24.13880561200159	26.197896258766857	25.939408850535962
44	21.822442538947765	22.22855025317186	30.199591158576407	25.74941604930397
45	22.145779608350573	20.68734565932514	28.30224498288394	28.86462974944035
46	26.59808093602209	23.79602883152149	27.706903831141812	21.89898640131461
47	19.182894269233923	22.00028552075645	35.68872770128454	23.12809250872509
48	21.481640104123954	23.040158190648892	30.167394137104637	25.310807568122517
49	22.00711979418206	20.136199475735292	34.08464779192219	23.772032938160457
50	22.098091567114082	21.475717067155088	30.4352976553886	25.990893710342228
51	20.91090233671402	21.685301452207167	28.03631581025627	29.367480400822544
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	144.0
1	561.0
2	978.0
3	2139.0
4	3300.0
5	2300.0
6	1300.0
7	1360.0
8	1420.0
9	1546.5
10	1673.0
11	1647.0
12	1621.0
13	1711.0
14	1801.0
15	1707.5
16	1614.0
17	1534.0
18	1454.0
19	1700.0
20	1946.0
21	2054.5
22	2163.0
23	2701.0
24	3239.0
25	3486.5
26	4648.0
27	5562.0
28	5920.0
29	6278.0
30	7667.5
31	9057.0
32	9849.0
33	10641.0
34	12550.0
35	14459.0
36	15840.5
37	17222.0
38	18772.5
39	20323.0
40	22804.0
41	25285.0
42	29971.5
43	34658.0
44	38146.5
45	41635.0
46	67623.0
47	93611.0
48	82767.0
49	71923.0
50	67913.5
51	63904.0
52	55062.0
53	46220.0
54	40249.0
55	34278.0
56	32712.0
57	31146.0
58	29975.5
59	28805.0
60	26363.0
61	23921.0
62	20168.5
63	16416.0
64	14295.5
65	12175.0
66	10598.0
67	9021.0
68	7340.0
69	5659.0
70	4527.0
71	3395.0
72	3006.0
73	2617.0
74	2202.0
75	1472.5
76	1158.0
77	760.0
78	362.0
79	352.0
80	342.0
81	245.0
82	148.0
83	88.0
84	28.0
85	20.5
86	13.0
87	10.0
88	7.0
89	5.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	658446.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.47284316444034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.94567812923272	19.046066534744828
2	11.665506311774559	6.176382380507936
3	4.704270362711209	3.7360623454553608
4	2.464327912744409	2.6095106535934143
5	1.421709993350232	1.88183528396391
6	0.9053116923100321	1.4379704667274527
7	0.5987121660931184	1.1094729291537835
8	0.4779056513614286	1.0121217084712644
9	0.34479040257805166	0.8214824026847706
>10	4.238726033471571	28.850932294142346
>50	1.1213856806167477	19.247362499865442
>100	0.09952442573069169	4.5497497928485275
>500	0.004629043057241474	0.7965355505626671
>1k	0.005786303821551842	3.745402066693803
>5k	0.0	0.0
>10k+	0.0017358911464655526	4.9791130905845185
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGC	11463	1.7409172506173627	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCG	10950	1.6630065335653947	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTC	10092	1.5326997202504078	No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	3412	0.5181897984041212	No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT	2875	0.4366341355251608	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCGTTGAT	2813	0.4272180254720964	No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	2709	0.411423260221795	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCT	2486	0.3775556385793216	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT	2295	0.3485479447061718	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTC	2261	0.34338427145126554	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCGTTGATCGTA	1997	0.30328986735434643	No Hit
TCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	1871	0.28415390176263505	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1732	0.263043590514636	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGC	912	0.13850794142572057	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCG	825	0.12529501280287222	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTCGTTGATCGT	693	0.10524781075441265	No Hit
TCCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG	686	0.10418470155487314	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5187274279136027E-4	0.0	0.0	0.4761210486509144	0.0
2	1.5187274279136027E-4	0.0	0.0	1.8425201155447828	0.0
3	1.5187274279136027E-4	0.0	0.0	2.4571491056214176	0.0
4	1.5187274279136027E-4	0.0	0.0	3.487605665460797	0.0
5	1.5187274279136027E-4	0.0	0.0	6.178486922238118	0.0
6	1.5187274279136027E-4	0.0	0.0	6.867989174510893	0.0
7	1.5187274279136027E-4	0.0	0.0	7.862148148823138	0.0
8	3.0374548558272053E-4	0.0	0.0	9.124818132390507	0.0
9	3.0374548558272053E-4	0.0	0.0	9.524699064160158	0.0
10	3.0374548558272053E-4	0.0	0.0	12.194166264203899	0.0
11	3.0374548558272053E-4	0.0	0.0	13.710919346461214	0.0
12	3.0374548558272053E-4	0.0	0.0	17.188045792669406	0.0
13	3.0374548558272053E-4	0.0	0.0	17.854615260780687	0.0
14	3.0374548558272053E-4	0.0	0.0	18.141502871913566	0.0
15	3.0374548558272053E-4	0.0	0.0	18.83070137870076	0.0
16	3.0374548558272053E-4	0.0	0.0	19.54662948821923	0.0
17	3.0374548558272053E-4	0.0	0.0	20.448753580399913	0.0
18	3.0374548558272053E-4	0.0	0.0	21.443975663911694	0.0
19	3.0374548558272053E-4	0.0	0.0	22.63830291322295	0.0
20	3.0374548558272053E-4	0.0	0.0	23.370025787991725	0.0
21	3.0374548558272053E-4	0.0	0.0	24.250432077953242	0.0
22	4.556182283740808E-4	0.0	0.0	25.276788073737254	0.0
23	4.556182283740808E-4	0.0	0.0	26.130009142739116	0.0
24	4.556182283740808E-4	0.0	0.0	26.785188155141043	0.0
25	4.556182283740808E-4	0.0	0.0	27.32843695610574	0.0
26	4.556182283740808E-4	0.0	0.0	27.834476935086553	0.0
27	4.556182283740808E-4	0.0	0.0	28.396557956157377	0.0
28	4.556182283740808E-4	0.0	0.0	28.97534497893525	0.0
29	4.556182283740808E-4	0.0	0.0	29.553372637999168	0.0
30	4.556182283740808E-4	0.0	0.0	30.115909277298368	0.0
31	4.556182283740808E-4	0.0	0.0	30.694848172819032	0.0
32	4.556182283740808E-4	0.0	0.0	31.23733761006977	0.0
33	4.556182283740808E-4	0.0	0.0	31.744440698250123	0.0
34	4.556182283740808E-4	0.0	0.0	32.29725748201067	0.0
35	4.556182283740808E-4	0.0	0.0	32.84415122880236	0.0
36	4.556182283740808E-4	0.0	0.0	33.36826406417535	0.0
37	6.074909711654411E-4	0.0	0.0	33.90179300960139	0.0
38	7.593637139568013E-4	0.0	0.0	34.44185248296747	0.0
39	7.593637139568013E-4	0.0	0.0	35.0390161076231	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCATC	20	7.031088E-4	45.000004	40
GGTGTAC	40	6.8066583E-9	45.000004	8
CAACCCG	20	7.031088E-4	45.000004	23
ATATGGA	20	7.031088E-4	45.000004	29
CGTTACG	20	7.031088E-4	45.000004	45
GGAACGT	35	1.2108649E-7	45.000004	8
ACTCGTC	20	7.031088E-4	45.000004	1
GCGATAT	20	7.031088E-4	45.000004	9
ACGCTAG	20	7.031088E-4	45.000004	37
ACGTCAT	20	7.031088E-4	45.000004	11
CATCGCA	20	7.031088E-4	45.000004	43
TTACGGT	20	7.031088E-4	45.000004	29
TTACGGG	40	6.8066583E-9	45.000004	3
CGATCGC	35	1.2108649E-7	45.000004	34
TATCGAA	20	7.031088E-4	45.000004	35
CAATAGT	20	7.031088E-4	45.000004	19
GGCTCGT	20	7.031088E-4	45.000004	8
GGTAGGC	20	7.031088E-4	45.000004	8
CTTACGA	20	7.031088E-4	45.000004	29
TATCCGG	20	7.031088E-4	45.000004	2
>>END_MODULE