SRR3549706_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549706_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	988688
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT	4882	0.49378570388231674	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	3846	0.3890003722104446	TruSeq Adapter, Index 19 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	3659	0.3700864175553865	TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	3570	0.3610845888692894	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2023	0.20461460035926402	No Hit
TCCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG	1356	0.13715145728480574	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGCG	25	3.890408E-5	45.000004	10
TCTACGA	25	3.890408E-5	45.000004	31
GTTCGTA	25	3.890408E-5	45.000004	30
AACGCGC	25	3.890408E-5	45.000004	15
AGTTCGT	50	2.1827873E-11	45.000004	29
ACGTCGT	25	3.890408E-5	45.000004	10
GCCCGTT	25	3.890408E-5	45.000004	13
TCGAACC	25	3.890408E-5	45.000004	11
CGGACTA	25	3.890408E-5	45.000004	41
CGTTGGT	20	7.0328085E-4	45.0	13
ATTCGGA	20	7.0328085E-4	45.0	38
CGCGATT	20	7.0328085E-4	45.0	25
TTATCCG	20	7.0328085E-4	45.0	1
TCTAGCG	35	1.2117016E-7	45.0	1
ATCCGAT	35	1.2117016E-7	45.0	38
AACGCTC	20	7.0328085E-4	45.0	14
ACTCGAA	20	7.0328085E-4	45.0	45
TACTCGA	20	7.0328085E-4	45.0	44
CGTAATG	20	7.0328085E-4	45.0	21
CGAGAGT	20	7.0328085E-4	45.0	37