SRR3549705_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549705_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1749995
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	7701	0.4400584001668576	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	7176	0.410058314452327	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6988	0.3993154266155046	No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	5342	0.30525801502290006	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	4050	0.23142923265495044	TruSeq Adapter, Index 13 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG	2822	0.16125760359315314	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCC	2683	0.15331472375635358	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT	2057	0.11754319298055138	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGA	40	6.8193913E-9	45.0	27
GCGAACC	25	3.891644E-5	45.0	10
TACGGAT	20	7.034298E-4	45.0	31
ATCGTCG	20	7.034298E-4	45.0	1
CGCAATA	20	7.034298E-4	45.0	43
ATACGGT	20	7.034298E-4	45.0	20
TATCGTC	20	7.034298E-4	45.0	44
CCGTTAG	20	7.034298E-4	45.0	1
CGCATCG	30	2.1661544E-6	44.999996	21
TATCGCC	30	2.1661544E-6	44.999996	14
CGTTTTT	4630	0.0	42.813175	1
TGGTCCG	80	0.0	42.1875	1
TATCGTG	70	0.0	41.785713	1
CCGATAA	65	0.0	41.538464	15
CGATAAC	65	0.0	41.538464	16
TATCCGG	85	0.0	39.705883	2
TTCACCG	160	0.0	39.375	25
ACGACGG	40	3.4596087E-7	39.375	27
CCGCGAG	115	0.0	39.130436	3
TCTTGCG	570	0.0	39.078945	1