##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549705_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1749995
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06949048425852	33.0	31.0	34.0	30.0	34.0
2	32.262979037082964	34.0	31.0	34.0	30.0	34.0
3	32.32209063454467	34.0	31.0	34.0	30.0	34.0
4	35.86779905085443	37.0	35.0	37.0	35.0	37.0
5	35.840316686619104	37.0	35.0	37.0	35.0	37.0
6	35.532500950002714	37.0	35.0	37.0	33.0	37.0
7	35.88362252463578	37.0	35.0	37.0	35.0	37.0
8	35.97822165206186	37.0	35.0	37.0	35.0	37.0
9	37.77393078265938	39.0	38.0	39.0	35.0	39.0
10	37.100812859465314	39.0	37.0	39.0	33.0	39.0
11	37.00585773102209	39.0	37.0	39.0	33.0	39.0
12	36.93244666413332	39.0	37.0	39.0	33.0	39.0
13	36.89615513187181	39.0	37.0	39.0	33.0	39.0
14	38.02035948674139	40.0	37.0	41.0	33.0	41.0
15	38.05077043077266	40.0	37.0	41.0	33.0	41.0
16	38.079513941468406	40.0	37.0	41.0	33.0	41.0
17	38.026188646253274	40.0	37.0	41.0	33.0	41.0
18	37.983883953954155	40.0	37.0	41.0	33.0	41.0
19	37.98518510052886	40.0	37.0	41.0	33.0	41.0
20	37.91171289060826	40.0	37.0	41.0	33.0	41.0
21	37.75499072854494	39.0	36.0	41.0	33.0	41.0
22	37.87265792187978	39.0	36.0	41.0	33.0	41.0
23	37.82199149140426	39.0	36.0	41.0	33.0	41.0
24	37.75853359581027	39.0	36.0	41.0	33.0	41.0
25	37.692079120226055	39.0	36.0	41.0	33.0	41.0
26	37.65159329026655	39.0	36.0	41.0	33.0	41.0
27	37.59684799099426	39.0	36.0	41.0	33.0	41.0
28	37.482416806905164	39.0	36.0	41.0	33.0	41.0
29	37.41989605684588	39.0	36.0	41.0	32.0	41.0
30	37.38079080225943	39.0	36.0	41.0	32.0	41.0
31	37.29940599830285	39.0	36.0	41.0	32.0	41.0
32	37.17920794059412	39.0	36.0	41.0	32.0	41.0
33	37.13009237169249	39.0	36.0	41.0	31.0	41.0
34	36.959085025957215	39.0	35.0	41.0	31.0	41.0
35	36.89719399198283	39.0	35.0	41.0	31.0	41.0
36	36.75078043080123	39.0	35.0	41.0	31.0	41.0
37	36.70291115117472	39.0	35.0	41.0	30.0	41.0
38	36.64179497655707	39.0	35.0	41.0	30.0	41.0
39	36.58930054085869	39.0	35.0	41.0	30.0	41.0
40	36.48810539458684	39.0	35.0	40.0	30.0	41.0
41	36.407410878316796	39.0	35.0	40.0	30.0	41.0
42	36.37708393452553	39.0	35.0	40.0	30.0	41.0
43	36.337858108166024	39.0	35.0	40.0	30.0	41.0
44	36.21180860516744	39.0	35.0	40.0	30.0	41.0
45	36.264831042374404	39.0	35.0	40.0	30.0	41.0
46	36.12024091497404	39.0	35.0	40.0	30.0	41.0
47	35.9058740167829	38.0	35.0	40.0	29.0	41.0
48	35.82030862945323	38.0	35.0	40.0	29.0	41.0
49	35.836761819319484	38.0	35.0	40.0	29.0	41.0
50	35.758062737322106	38.0	35.0	40.0	28.0	41.0
51	34.823365209614884	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	17.0
10	31.0
11	17.0
12	20.0
13	19.0
14	24.0
15	32.0
16	69.0
17	108.0
18	284.0
19	581.0
20	1022.0
21	1670.0
22	2728.0
23	4250.0
24	6840.0
25	10629.0
26	14757.0
27	17433.0
28	20040.0
29	23928.0
30	29592.0
31	38016.0
32	49812.0
33	67436.0
34	105341.0
35	130243.0
36	154025.0
37	232656.0
38	394445.0
39	443805.0
40	117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.39692113406038	4.373841068117337	59.19005482872808	14.039182969094197
2	20.266972191349115	4.04555441586976	54.63747039277255	21.050003000008573
3	18.928854082440235	3.8442395549701573	55.110614601756005	22.116291760833604
4	17.54045011557176	4.267155049014425	53.96998277137934	24.22241206403447
5	16.75016214332041	5.290415115471758	54.083354523870064	23.876068217337764
6	19.848456709876313	5.478187080534515	57.105591730262084	17.56776447932708
7	82.98355138157537	1.4827470935631244	11.086660247600708	4.447041277260793
8	85.83990239972114	1.7059477312792324	7.992365692473408	4.461784176526218
9	81.19908914025469	3.6377246792133695	10.387686821962347	4.775499358569596
10	36.629761799319425	25.7369878199652	20.527258649310426	17.105991731404945
11	23.808068023051497	28.6140817545193	27.549164426184074	20.02868579624513
12	22.137891822548063	23.96286846533847	32.51380718230624	21.38543252980723
13	22.179491941405548	24.378469652770438	33.49655284729385	19.94548555853017
14	19.592341692404837	26.115274615070327	33.4320383772525	20.86034531527233
15	17.73307923736925	27.073505924302644	34.42569835913817	20.76771647918994
16	19.575655930445514	26.294189411969747	32.1242632121806	22.00589144540413
17	20.24525784359384	26.496532847236708	30.05820016628619	23.200009142883264
18	20.68754482155663	25.440815545187274	32.548835853816726	21.32280377943937
19	21.317375192500553	28.249852142434694	30.192200549144427	20.24057211592033
20	22.77532221520633	28.10853745296415	30.25311500890003	18.863025322929495
21	22.14303469438484	28.247394992557123	30.359458169880487	19.250112143177553
22	20.649030425801215	25.171329060940174	30.17602907436878	24.003611438889823
23	19.308798025137214	26.533732953522726	29.79031368661053	24.367155334729528
24	19.97131420375487	26.23001780005086	30.846430989802826	22.952237006391446
25	19.55257015020043	27.32047805850874	28.64265326472361	24.48429852656722
26	18.69319626627505	27.852422435492674	30.504030011514317	22.95035128671796
27	18.285023671496205	27.66807905165443	30.6926019788628	23.354295297986567
28	16.93330552373007	27.08819168054766	32.36043531552947	23.6180674801928
29	18.26639504684299	24.943099837428107	32.57077877365364	24.219726342075262
30	20.190400544001555	26.43058980168515	30.821345203843443	22.557664450469858
31	18.61845319558056	29.233912096891707	28.609738884968245	23.53789582255949
32	18.75731073517353	29.243512124320354	28.480424229783512	23.5187529107226
33	19.53628438938397	26.949734142097547	27.74585070243058	25.768130766087904
34	17.614793185123386	27.198877711079174	29.088425966931336	26.09790313686611
35	17.47490707116306	25.592415978331367	30.29380083943097	26.638876111074605
36	18.349823856639592	27.431678376223935	28.78453938439824	25.433958382738236
37	16.802333720953488	29.307855165300474	28.976539932971235	24.913271180774803
38	17.57125020357201	29.41922691207689	29.56059874456784	23.448924139783255
39	19.715713473467066	25.799273712210606	30.077171649061857	24.40784116526047
40	20.542058691596264	25.177443364123896	29.285855102443147	24.994642841836693
41	17.367821050917286	27.327678079080226	28.27990937116963	27.024591498832855
42	17.56547875851074	27.287849393855414	28.138537538678683	27.008134308955167
43	18.256395018271483	25.87875965359901	29.515341472404206	26.349503855725303
44	18.53302438006966	25.44104411726891	28.766939334112386	27.25899216854905
45	20.15805759445027	24.179554798727995	28.490424258355024	27.17196334846671
46	19.644798985139957	25.623673210494886	29.720999202854863	25.01052860151029
47	16.85616244617842	26.53099008854311	31.143460409886885	25.469387055391586
48	16.476504218583482	26.17264620756059	31.100774573641637	26.250075000214284
49	19.234112097463136	24.289612256035017	31.05140300400858	25.424872642493263
50	18.893482552807296	23.27960937031249	29.810542315835185	28.01636576104503
51	18.294909414026897	23.752982151377573	27.498764282183664	30.453344152411866
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	256.0
1	907.0
2	1558.0
3	6556.0
4	11554.0
5	8056.0
6	4558.0
7	4454.5
8	4351.0
9	4630.0
10	4909.0
11	5032.5
12	5156.0
13	5320.0
14	5484.0
15	5475.0
16	5466.0
17	5542.5
18	5619.0
19	6011.5
20	6404.0
21	6763.0
22	7122.0
23	8267.0
24	9412.0
25	10767.5
26	14585.0
27	17047.0
28	20562.0
29	24077.0
30	27856.0
31	31635.0
32	35272.5
33	38910.0
34	44345.0
35	49780.0
36	57103.0
37	64426.0
38	73638.5
39	82851.0
40	90048.0
41	97245.0
42	107954.5
43	118664.0
44	132977.0
45	147290.0
46	165811.5
47	184333.0
48	203719.5
49	223106.0
50	203818.0
51	184530.0
52	154516.5
53	124503.0
54	101654.5
55	78806.0
56	68074.5
57	57343.0
58	50337.0
59	43331.0
60	38028.0
61	32725.0
62	28021.0
63	23317.0
64	19547.5
65	15778.0
66	12470.0
67	9162.0
68	7685.5
69	6209.0
70	5191.0
71	4173.0
72	3640.5
73	3108.0
74	2489.0
75	1367.5
76	865.0
77	687.0
78	509.0
79	315.0
80	121.0
81	130.0
82	139.0
83	106.5
84	74.0
85	48.0
86	22.0
87	22.0
88	22.0
89	21.5
90	21.0
91	25.0
92	29.0
93	15.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1749995.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.903748226219626
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.33801928291253	22.440464579330218
2	12.880777277126596	8.218901504149152
3	5.341593442218021	5.112505569220486
4	2.721464887229329	3.4729972227464683
5	1.5965506686418045	2.546797528137536
6	1.0001394173504305	1.9144917697359674
7	0.728380868587556	1.6266655890948785
8	0.5379334888714209	1.3729675673124582
9	0.3960416143295578	1.1371690755608206
>10	3.566964754401975	25.862633640621823
>50	0.787721318140552	16.709184406296657
>100	0.09587838042791916	5.514202404650953
>500	0.005629204098580189	1.250950126764633
>1k	0.0021790467478374925	1.2438822846183224
>5k	7.263489159458307E-4	1.5761867317596152
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	7701	0.4400584001668576	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	7176	0.410058314452327	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6988	0.3993154266155046	No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	5342	0.30525801502290006	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	4050	0.23142923265495044	TruSeq Adapter, Index 13 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG	2822	0.16125760359315314	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCC	2683	0.15331472375635358	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT	2057	0.11754319298055138	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2857208163451896E-4	0.0	0.0	0.4421155489015683	0.0
2	2.2857208163451896E-4	0.0	0.0	1.537890108257452	0.0
3	2.2857208163451896E-4	0.0	0.0	2.2320063771610776	0.0
4	2.2857208163451896E-4	0.0	0.0	3.118866053903011	0.0
5	2.2857208163451896E-4	0.0	0.0	4.5084700242000695	0.0
6	2.2857208163451896E-4	0.0	0.0	5.635787530821517	0.0
7	2.2857208163451896E-4	0.0	0.0	6.516190046257275	0.0
8	2.2857208163451896E-4	0.0	0.0	7.8952225577787365	0.0
9	2.2857208163451896E-4	0.0	0.0	8.546767276477933	0.0
10	2.857151020431487E-4	0.0	0.0	9.871685347672422	0.0
11	2.857151020431487E-4	0.0	0.0	12.410264029325798	0.0
12	2.857151020431487E-4	0.0	0.0	14.40901259717885	0.0
13	2.857151020431487E-4	0.0	0.0	15.138900396858277	0.0
14	2.857151020431487E-4	0.0	0.0	15.355701016288618	0.0
15	2.857151020431487E-4	0.0	0.0	15.660216172046205	0.0
16	2.857151020431487E-4	0.0	0.0	16.49856142446121	0.0
17	2.857151020431487E-4	0.0	0.0	17.81016517190049	0.0
18	2.857151020431487E-4	0.0	0.0	19.20554058725882	0.0
19	2.857151020431487E-4	0.0	0.0	20.196629133226097	0.0
20	2.857151020431487E-4	0.0	0.0	21.19428912654036	0.0
21	2.857151020431487E-4	0.0	0.0	22.739150683287665	0.0
22	2.857151020431487E-4	0.0	0.0	24.332698093423126	0.0
23	2.857151020431487E-4	0.0	0.0	26.011902891151117	0.0
24	2.857151020431487E-4	0.0	0.0	27.150191857691024	0.0
25	2.857151020431487E-4	0.0	0.0	28.137108963168465	0.0
26	2.857151020431487E-4	0.0	0.0	28.982939951257002	0.0
27	2.857151020431487E-4	0.0	0.0	29.84334240954974	0.0
28	2.857151020431487E-4	0.0	0.0	30.796145131843232	0.0
29	2.857151020431487E-4	0.0	0.0	31.775233643524697	0.0
30	2.857151020431487E-4	0.0	0.0	32.80266515047186	0.0
31	2.857151020431487E-4	0.0	0.0	33.76986791390833	0.0
32	2.857151020431487E-4	0.0	0.0	34.68741339260969	0.0
33	2.857151020431487E-4	0.0	0.0	35.590215972045634	0.0
34	2.857151020431487E-4	0.0	0.0	36.44610413172609	0.0
35	3.4285812245177846E-4	0.0	0.0	37.36267817908051	0.0
36	3.4285812245177846E-4	0.0	0.0	38.24290926545505	0.0
37	3.4285812245177846E-4	0.0	0.0	39.08216880619659	0.0
38	3.4285812245177846E-4	0.0	0.0	39.94462841322404	0.0
39	3.4285812245177846E-4	0.0	0.0	40.867888193966266	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGA	40	6.8193913E-9	45.0	27
GCGAACC	25	3.891644E-5	45.0	10
TACGGAT	20	7.034298E-4	45.0	31
ATCGTCG	20	7.034298E-4	45.0	1
CGCAATA	20	7.034298E-4	45.0	43
ATACGGT	20	7.034298E-4	45.0	20
TATCGTC	20	7.034298E-4	45.0	44
CCGTTAG	20	7.034298E-4	45.0	1
CGCATCG	30	2.1661544E-6	44.999996	21
TATCGCC	30	2.1661544E-6	44.999996	14
CGTTTTT	4630	0.0	42.813175	1
TGGTCCG	80	0.0	42.1875	1
TATCGTG	70	0.0	41.785713	1
CCGATAA	65	0.0	41.538464	15
CGATAAC	65	0.0	41.538464	16
TATCCGG	85	0.0	39.705883	2
TTCACCG	160	0.0	39.375	25
ACGACGG	40	3.4596087E-7	39.375	27
CCGCGAG	115	0.0	39.130436	3
TCTTGCG	570	0.0	39.078945	1
>>END_MODULE