FastQCFastQC Report
Sat 18 Jun 2016
SRR3549704_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549704_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2490101
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75420.3028792808002567No Hit
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC65590.26340297040160215TruSeq Adapter, Index 21 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT51080.20513224162393412TruSeq Adapter, Index 15 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC41700.1674630868386463TruSeq Adapter, Index 21 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC37420.1502750290048476TruSeq Adapter, Index 21 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACT302.1665273E-645.00000436
ACTACGT207.034873E-445.00000414
TACGGGT1950.042.6923074
TATAGCG603.6379788E-1241.25000434
CGTTTTT45000.040.651
TCTAGCG1000.040.5000041
TCTTGCG7650.040.2941171
CGTACCG403.460391E-739.37500426
CGTTCAT3800.039.07894517
TTTGGGA172450.038.7242664
TCGTTCA3900.038.65384716
TCGTCTA356.2512263E-638.57142612
TTGGGAC67200.038.43755
AACGACG1300.038.07692711
TATTGCG1950.038.0769231
TGGGATC45300.037.8476836
TTGGGAT120900.037.7977685
ACACGAC4850.037.57731626
CACTACG301.1403044E-437.50000434
TGGGCGA29100.037.5000046
Produced by FastQC (version 0.11.5)