FastQCFastQC Report
Sat 18 Jun 2016
SRR3549703_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549703_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1734739
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44670.2575027136647069No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT33580.1935737883335764Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC26930.15523949135864243TruSeq Adapter, Index 22 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC25740.14837966979470688TruSeq Adapter, Index 22 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC22600.13027896415541473TruSeq Adapter, Index 22 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCCGA1350.045.00000412
TATCACG253.89163E-545.016
GTCCGAT207.034282E-445.015
TATCGGC207.034282E-445.032
TCGGTAA207.034282E-445.05
TTTAGCG1200.041.2500041
CGCATCG556.184564E-1140.90909221
ATAAGCG501.0822987E-940.533
TTTGTCG5000.040.0500031
TTTCGCG5400.039.1666681
CGTTTTT26300.038.9258541
TCCGATA1450.038.79310214
CCGATAA1450.038.79310215
CGATAAC1450.038.79310216
CGGTCTA356.2501294E-638.57142631
TGGGCGA21500.038.5116276
GGCGATT15850.038.470038
TAGCGGG3100.038.467743
TTTACGG1350.038.3333362
GCCGATT2000.038.259
Produced by FastQC (version 0.11.5)