##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549703_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1734739
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23230526321251	33.0	31.0	34.0	30.0	34.0
2	32.414480218638076	34.0	31.0	34.0	31.0	34.0
3	32.47633620965459	34.0	31.0	34.0	30.0	34.0
4	35.96442116076251	37.0	35.0	37.0	35.0	37.0
5	35.91663010977444	37.0	35.0	37.0	35.0	37.0
6	35.54001725908047	37.0	35.0	37.0	33.0	37.0
7	35.858668076292744	37.0	35.0	37.0	35.0	37.0
8	35.95452975923179	37.0	35.0	37.0	35.0	37.0
9	37.72348232212454	39.0	38.0	39.0	35.0	39.0
10	37.29255179021167	39.0	37.0	39.0	34.0	39.0
11	37.19797560324637	39.0	37.0	39.0	34.0	39.0
12	36.85949010196923	39.0	35.0	39.0	33.0	39.0
13	36.72652773702557	39.0	35.0	39.0	33.0	39.0
14	37.61928509130192	40.0	36.0	41.0	33.0	41.0
15	37.74605055861429	40.0	36.0	41.0	33.0	41.0
16	37.88135621554597	40.0	36.0	41.0	33.0	41.0
17	37.86368727514629	40.0	36.0	41.0	33.0	41.0
18	37.84963847587447	40.0	36.0	41.0	33.0	41.0
19	37.87981016164391	40.0	36.0	41.0	33.0	41.0
20	37.794656717811726	40.0	36.0	41.0	33.0	41.0
21	37.6528532534289	40.0	35.0	41.0	33.0	41.0
22	37.77051879274058	40.0	36.0	41.0	33.0	41.0
23	37.734973964383116	39.0	35.0	41.0	33.0	41.0
24	37.688223415741504	40.0	35.0	41.0	33.0	41.0
25	37.59993866512484	39.0	35.0	41.0	33.0	41.0
26	37.53145804642658	39.0	35.0	41.0	33.0	41.0
27	37.448811031515405	39.0	35.0	41.0	33.0	41.0
28	37.30922692116797	39.0	35.0	41.0	33.0	41.0
29	37.22769304200805	39.0	35.0	41.0	32.0	41.0
30	37.180047834285155	39.0	35.0	41.0	32.0	41.0
31	37.07468904544142	39.0	35.0	41.0	32.0	41.0
32	36.96901724121035	39.0	35.0	41.0	31.0	41.0
33	36.91584267143357	39.0	35.0	41.0	31.0	41.0
34	36.69128381848797	39.0	35.0	41.0	31.0	41.0
35	36.6418959855056	39.0	35.0	41.0	31.0	41.0
36	36.50897512536468	39.0	35.0	41.0	30.0	41.0
37	36.449786394379785	39.0	35.0	41.0	30.0	41.0
38	36.38620103658245	39.0	35.0	41.0	30.0	41.0
39	36.32636206368797	39.0	35.0	41.0	30.0	41.0
40	36.154659577031474	38.0	35.0	40.0	30.0	41.0
41	36.008461791658576	38.0	35.0	40.0	30.0	41.0
42	35.9732784009583	38.0	35.0	40.0	30.0	41.0
43	35.95038619642494	38.0	35.0	40.0	30.0	41.0
44	35.83911470255756	38.0	35.0	40.0	29.0	41.0
45	35.86892264484744	38.0	35.0	40.0	30.0	41.0
46	35.7799138660052	38.0	35.0	40.0	29.0	41.0
47	35.56063304047468	38.0	34.0	40.0	29.0	41.0
48	35.46286963053232	38.0	34.0	40.0	28.0	41.0
49	35.440045447759	37.0	34.0	40.0	28.0	41.0
50	35.332241334287176	37.0	34.0	40.0	28.0	41.0
51	34.333110629322334	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	39.0
10	54.0
11	50.0
12	34.0
13	50.0
14	60.0
15	86.0
16	161.0
17	270.0
18	480.0
19	843.0
20	1531.0
21	2289.0
22	3455.0
23	5192.0
24	7253.0
25	10490.0
26	14387.0
27	17842.0
28	20873.0
29	25248.0
30	30992.0
31	40123.0
32	53287.0
33	77282.0
34	132887.0
35	138367.0
36	143635.0
37	211425.0
38	350408.0
39	445475.0
40	161.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.752807771082566	4.819284053681851	53.03691218102551	16.39099599421008
2	21.569988338303343	4.564029516832215	51.781910708181464	22.08407143668298
3	22.730393448236306	4.95849807953819	49.97362715659243	22.337481315633074
4	20.194161773039056	5.5353571920617455	48.828555765449444	25.441925269449754
5	18.795392275149172	6.50345671596707	49.05337344695657	25.64777756192718
6	22.414784010735907	6.979205517371778	52.44915805778275	18.156852414109558
7	83.78562999967141	3.15373090707017	8.731399939702746	4.3292391535556645
8	85.05619577354288	3.438269388075094	7.42590095685864	4.079633881523388
9	79.4510298090952	5.646497830509373	9.408735262192181	5.493737098203246
10	41.71543961368252	26.916613969017817	16.711217076459338	14.656729340840322
11	33.249900993751794	24.423212944425646	24.078665436126126	18.24822062569643
12	31.15909655573547	21.12386935441009	28.411132741005996	19.305901348848444
13	28.589084582752793	20.549373709820323	29.643710091258686	21.217831616168194
14	21.453140789478994	23.358211235234812	33.05690366101183	22.131744314274364
15	20.188570153781058	24.473537517747626	35.00901288320606	20.32887944526525
16	25.98281355293217	23.504861538248694	30.312340934284638	20.199983974534497
17	25.338624427075196	22.95388528187814	29.03312832650906	22.674361964537603
18	25.272966134963244	23.459148609675577	29.673282263210776	21.594602992150406
19	26.43815582632315	25.71170648725831	27.33316078095898	20.516976905459554
20	27.18506933896108	27.328952655125644	26.56509134803564	18.920886657877638
21	26.67519436641477	25.391081886093524	29.37894403711452	18.554779710377183
22	23.681256949892752	23.10191907831668	30.705483649125316	22.511340322665255
23	23.621305568157517	24.45088281291883	30.158542581910012	21.769269037013636
24	24.56133170465413	24.453649799768147	28.448487063471795	22.536531432105924
25	24.907896807531277	25.0013979048145	25.846885323959395	24.243819963694826
26	22.063261389753734	25.548742490945326	27.646464396084948	24.741531723216
27	21.76304331660267	25.16349721773708	30.118882437069782	22.954577028590467
28	20.211916605322184	26.541860187613235	28.765825867753016	24.480397339311562
29	23.153050689469712	26.439942838663338	27.954522265309073	22.452484206557873
30	25.34254432511173	23.659928092929253	29.29512739380391	21.702400188155107
31	24.584678156195256	25.396097049757916	28.03954946536626	21.979675328680568
32	26.57581342207675	25.473976200454363	26.376993887841344	21.573216489627548
33	27.36976571115309	23.970061202290374	26.390655885409853	22.269517201146684
34	22.856176058761577	24.381246977210978	30.47588138619124	22.286695577836205
35	22.65032376628415	25.517959762246655	28.466760705789167	23.364955765680023
36	25.23820586266868	26.758549845250496	26.133960209576195	21.86928408250463
37	23.840762212644094	28.461111440971816	25.874151673537053	21.823974672847037
38	24.74954445596715	26.40137795945096	25.61803245329701	23.23104513128488
39	23.66407857320323	23.82565907609156	26.52105014068399	25.98921221002122
40	25.43858182700683	22.667617434092392	29.03111073193143	22.86269000696935
41	22.190485139263025	23.951384041057473	28.57824721759296	25.27988360208654
42	22.086434904616777	24.641113158809482	28.29808979909946	24.97436213747428
43	22.084359664479784	23.566369350086667	28.217847180469224	26.13142380496432
44	23.14711319685555	23.686733278032026	27.860444712432248	25.305708812680177
45	23.375735485280494	22.09606171303003	27.305894431381322	27.222308370308156
46	22.57555747579319	24.493944045761353	28.18383630044635	24.74666217799911
47	20.832182824044423	25.370329484723637	30.037717489489772	23.759770201742164
48	21.49954546476444	25.473918554895004	28.92694520616646	24.0995907741741
49	23.601475495737397	23.85004314770118	28.8652644576504	23.683216898911017
50	21.78529450251594	24.09567087613756	27.864018737112616	26.255015884233885
51	20.74871205408998	24.23119558619481	27.289868965879016	27.73022339383619
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	397.0
1	1540.5
2	2684.0
3	5756.5
4	8829.0
5	6136.0
6	3443.0
7	3477.0
8	3511.0
9	3604.5
10	3698.0
11	3763.5
12	3829.0
13	3806.0
14	3783.0
15	3760.5
16	3738.0
17	3873.5
18	4009.0
19	4149.0
20	4289.0
21	4840.5
22	5392.0
23	6906.5
24	8421.0
25	10618.5
26	13919.5
27	15023.0
28	16920.0
29	18817.0
30	22523.5
31	26230.0
32	31123.5
33	36017.0
34	40848.0
35	45679.0
36	48404.5
37	51130.0
38	57466.0
39	63802.0
40	67225.0
41	70648.0
42	76471.5
43	82295.0
44	87723.0
45	93151.0
46	100745.0
47	108339.0
48	120766.0
49	133193.0
50	146091.0
51	158989.0
52	156677.0
53	154365.0
54	141153.5
55	127942.0
56	118620.5
57	109299.0
58	102994.0
59	96689.0
60	87262.5
61	77836.0
62	69824.5
63	61813.0
64	53414.5
65	45016.0
66	37962.5
67	30909.0
68	26327.5
69	21746.0
70	18187.0
71	14628.0
72	12055.5
73	9483.0
74	7956.5
75	4782.5
76	3135.0
77	2504.0
78	1873.0
79	1354.5
80	836.0
81	569.5
82	303.0
83	222.0
84	141.0
85	104.5
86	68.0
87	62.0
88	56.0
89	35.5
90	15.0
91	9.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1734739.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.52166416552846
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.55291435990236	17.791182001638727
2	10.683437593854336	5.239513376197549
3	4.290467037876282	3.156281754482156
4	2.128695619286635	2.0879663634711396
5	1.2936833254145357	1.5861634021179658
6	0.8475680121884799	1.247026689139826
7	0.6309257275138138	1.0829944162439824
8	0.4669139054134519	0.9159604786211192
9	0.36975643537797315	0.8160338818243426
>10	4.88106102666175	30.49935948860795
>50	1.7100259538474998	28.174620561668938
>100	0.13882621081011773	5.571095489583306
>500	0.0033394619141435215	0.5639238755762591
>1k	0.0023853299386739435	1.2678782208267805
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4467	0.2575027136647069	No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	3358	0.1935737883335764	Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	2693	0.15523949135864243	TruSeq Adapter, Index 22 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	2574	0.14837966979470688	TruSeq Adapter, Index 22 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	2260	0.13027896415541473	TruSeq Adapter, Index 22 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.305822374432119E-4	0.0	0.0	0.21876489777424732	0.0
2	2.305822374432119E-4	0.0	0.0	0.7319833127634763	0.0
3	2.305822374432119E-4	0.0	0.0	1.0604477100013316	0.0
4	2.305822374432119E-4	0.0	0.0	1.4788968254014005	0.0
5	2.305822374432119E-4	0.0	0.0	2.1655130829479248	0.0
6	2.305822374432119E-4	0.0	0.0	2.6799420546837305	0.0
7	2.305822374432119E-4	0.0	0.0	3.1309032655632922	0.0
8	2.305822374432119E-4	0.0	0.0	3.802474032116647	0.0
9	2.305822374432119E-4	0.0	0.0	4.1531319697084115	0.0
10	2.305822374432119E-4	0.0	0.0	4.7999151457366205	0.0
11	2.305822374432119E-4	0.0	0.0	6.033241888261001	0.0
12	2.882277968040149E-4	0.0	0.0	7.111732658342263	0.0
13	2.882277968040149E-4	0.0	0.0	7.452648496402053	0.0
14	2.882277968040149E-4	0.0	0.0	7.597915305991276	0.0
15	2.882277968040149E-4	0.0	0.0	7.80497815521528	0.0
16	2.882277968040149E-4	0.0	0.0	8.24429496310396	0.0
17	2.882277968040149E-4	0.0	0.0	8.847959260730288	0.0
18	2.882277968040149E-4	0.0	0.0	9.52143233074255	0.0
19	2.882277968040149E-4	0.0	0.0	9.992223614042228	0.0
20	2.882277968040149E-4	0.0	0.0	10.55092437536713	0.0
21	2.882277968040149E-4	0.0	0.0	11.293456825493633	0.0
22	2.882277968040149E-4	0.0	0.0	12.062736815163548	0.0
23	2.882277968040149E-4	0.0	0.0	12.763937399228357	0.0
24	2.882277968040149E-4	0.0	0.0	13.266318449057755	0.0
25	2.882277968040149E-4	0.0	0.0	13.747831806398542	0.0
26	2.882277968040149E-4	0.0	0.0	14.16483978281459	0.0
27	2.882277968040149E-4	0.0	0.0	14.621911422986397	0.0
28	3.4587335616481787E-4	0.0	0.0	15.10019662900298	0.0
29	3.4587335616481787E-4	0.0	0.0	15.632553369699995	0.0
30	4.0351891552562087E-4	0.0	0.0	16.243365716687062	0.0
31	4.0351891552562087E-4	0.0	0.0	16.74078924841143	0.0
32	4.0351891552562087E-4	0.0	0.0	17.244957310581015	0.0
33	4.0351891552562087E-4	0.0	0.0	17.754659346449234	0.0
34	4.0351891552562087E-4	0.0	0.0	18.288630162808353	0.0
35	4.0351891552562087E-4	0.0	0.0	18.85984001051455	0.0
36	4.0351891552562087E-4	0.0	0.0	19.37669009574351	0.0
37	4.0351891552562087E-4	0.0	0.0	19.911871468849203	0.0
38	4.0351891552562087E-4	0.0	0.0	20.53905515469474	0.0
39	4.0351891552562087E-4	0.0	0.0	21.715946894604894	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCCGA	135	0.0	45.000004	12
TATCACG	25	3.89163E-5	45.0	16
GTCCGAT	20	7.034282E-4	45.0	15
TATCGGC	20	7.034282E-4	45.0	32
TCGGTAA	20	7.034282E-4	45.0	5
TTTAGCG	120	0.0	41.250004	1
CGCATCG	55	6.184564E-11	40.909092	21
ATAAGCG	50	1.0822987E-9	40.5	33
TTTGTCG	500	0.0	40.050003	1
TTTCGCG	540	0.0	39.166668	1
CGTTTTT	2630	0.0	38.925854	1
TCCGATA	145	0.0	38.793102	14
CCGATAA	145	0.0	38.793102	15
CGATAAC	145	0.0	38.793102	16
CGGTCTA	35	6.2501294E-6	38.571426	31
TGGGCGA	2150	0.0	38.511627	6
GGCGATT	1585	0.0	38.47003	8
TAGCGGG	310	0.0	38.46774	3
TTTACGG	135	0.0	38.333336	2
GCCGATT	200	0.0	38.25	9
>>END_MODULE