FastQCFastQC Report
Sat 18 Jun 2016
SRR3549702_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549702_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645237
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22520.34901904261534905No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC17080.2647089364063127No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT11220.17388959405613752No Hit
TCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC11200.173579630430369No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7780.12057585042395275No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA6870.10647250545148526No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACC600.045.00000424
ACCTACG302.1640153E-645.00000439
TCGACCC600.045.00000425
CGTCATA351.2108285E-745.00000438
ACGACTT351.2108285E-745.00000413
AACGTCG207.0309837E-445.011
TTCACGT207.0309837E-445.028
GTTAGCG253.8888975E-545.01
TCTACCG207.0309837E-445.01
CAGATAG207.0309837E-445.011
GTCAATC207.0309837E-445.010
TTAGTGT453.8380676E-1045.021
ACGTAGC207.0309837E-445.022
AACTCGC253.8888975E-545.038
GATTCGC406.8066583E-945.011
TCGCGAT207.0309837E-445.013
GTACGTA207.0309837E-445.020
TACGTAG207.0309837E-445.021
TAGCGGG1000.042.753
CGGGTAT950.042.631586