##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549702_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	645237
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.225084736306194	33.0	31.0	34.0	30.0	34.0
2	32.433849887715674	34.0	31.0	34.0	31.0	34.0
3	32.47763070003735	34.0	31.0	34.0	30.0	34.0
4	35.97105559662574	37.0	35.0	37.0	35.0	37.0
5	35.930022611846496	37.0	35.0	37.0	35.0	37.0
6	35.539118184481055	37.0	35.0	37.0	33.0	37.0
7	35.86490390352692	37.0	35.0	37.0	35.0	37.0
8	35.94463119752897	37.0	35.0	37.0	35.0	37.0
9	37.68097303781401	39.0	38.0	39.0	35.0	39.0
10	37.24235126007963	39.0	37.0	39.0	34.0	39.0
11	37.170515639989645	39.0	37.0	39.0	34.0	39.0
12	36.90716279444607	39.0	35.0	39.0	33.0	39.0
13	36.78811351487903	39.0	35.0	39.0	33.0	39.0
14	37.92583345344424	40.0	37.0	41.0	33.0	41.0
15	38.00088029669718	40.0	37.0	41.0	33.0	41.0
16	38.052298612757795	40.0	37.0	41.0	33.0	41.0
17	37.982476206417175	40.0	37.0	41.0	33.0	41.0
18	37.924726883300245	40.0	36.0	41.0	33.0	41.0
19	37.95087076531569	40.0	36.0	41.0	33.0	41.0
20	37.8605396156761	40.0	36.0	41.0	33.0	41.0
21	37.70200561964054	40.0	36.0	41.0	33.0	41.0
22	37.746888352651816	40.0	36.0	41.0	33.0	41.0
23	37.68699408124456	39.0	36.0	41.0	33.0	41.0
24	37.629108374132294	39.0	35.0	41.0	33.0	41.0
25	37.538120101606076	39.0	35.0	41.0	33.0	41.0
26	37.46376292742047	39.0	35.0	41.0	33.0	41.0
27	37.36179109381514	39.0	35.0	41.0	33.0	41.0
28	37.24766713626156	39.0	35.0	41.0	32.0	41.0
29	37.14318149765125	39.0	35.0	41.0	32.0	41.0
30	37.08602110542328	39.0	35.0	41.0	32.0	41.0
31	37.01667759288448	39.0	35.0	41.0	32.0	41.0
32	36.88381478433506	39.0	35.0	41.0	31.0	41.0
33	36.77435267971303	39.0	35.0	41.0	31.0	41.0
34	36.621501866755935	39.0	35.0	41.0	31.0	41.0
35	36.54375214068629	39.0	35.0	41.0	30.0	41.0
36	36.38407592868977	39.0	35.0	41.0	30.0	41.0
37	36.334791712192576	39.0	35.0	41.0	30.0	41.0
38	36.25071562852099	39.0	35.0	41.0	30.0	41.0
39	36.196687418731415	39.0	35.0	41.0	30.0	41.0
40	36.0420574145624	38.0	35.0	40.0	29.0	41.0
41	35.91137520012027	38.0	35.0	40.0	29.0	41.0
42	35.84178991595336	38.0	35.0	40.0	29.0	41.0
43	35.77911527082297	38.0	35.0	40.0	29.0	41.0
44	35.62796305853508	38.0	35.0	40.0	28.0	41.0
45	35.68491267549753	38.0	35.0	40.0	28.0	41.0
46	35.56524811813334	38.0	34.0	40.0	28.0	41.0
47	35.32076120867216	38.0	34.0	40.0	27.0	41.0
48	35.22586274500687	37.0	34.0	40.0	27.0	41.0
49	35.20885659067908	37.0	34.0	40.0	27.0	41.0
50	35.1074349425095	37.0	34.0	40.0	27.0	41.0
51	34.15874632111922	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	18.0
10	19.0
11	20.0
12	16.0
13	8.0
14	23.0
15	16.0
16	36.0
17	81.0
18	165.0
19	312.0
20	534.0
21	910.0
22	1285.0
23	2044.0
24	3006.0
25	4688.0
26	6131.0
27	7536.0
28	8634.0
29	9799.0
30	11966.0
31	14726.0
32	19026.0
33	26247.0
34	45783.0
35	55925.0
36	53584.0
37	79336.0
38	129547.0
39	163750.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.28215988853708	5.207233931098186	54.41039494015377	20.10021124021096
2	21.452892503064767	4.61845802395089	51.216684722047866	22.711964750936477
3	20.513702716986163	4.713616857061824	51.92882615225103	22.84385427370098
4	19.6764909637854	4.761971182681712	49.277552279240034	26.283985574292856
5	18.65593572594256	5.548658864882206	49.55760441512189	26.237800994053345
6	21.98742477570257	6.833303111879821	53.01710844232429	18.162163670093314
7	83.19392719264394	2.5121002050409387	9.938208751203046	4.355763851112072
8	84.25415777458515	3.186736036526114	8.390560367740846	4.168545821147888
9	77.80458963140676	6.0525047385689295	10.395095135585839	5.747810494438477
10	38.2896517093719	28.476048335727803	18.075993782129668	15.158306172770624
11	30.749166585301214	24.90836700313218	25.656309232111614	18.68615717945499
12	29.573009607322582	19.877192411470514	30.66098193377007	19.888816047436833
13	26.546679747131673	21.75123249286696	30.344664053673302	21.357423706328063
14	20.46224875510859	26.024391037711723	30.3338153267714	23.179544880408283
15	18.304591956133947	25.743873956391216	34.9532032415996	20.998330845875238
16	21.255600655263105	23.036961612554766	34.343659771525815	21.363777960656314
17	20.67054431162503	23.365213092243625	31.14328533546588	24.82095726066546
18	21.703188130872842	23.880682601896666	31.697965243778643	22.71816402345185
19	24.640713412281066	25.736899774811427	29.17532627546158	20.447060537445928
20	27.138555290536654	25.471260947527806	28.518048407019435	18.8721353549161
21	25.80834639055107	25.130455940995322	29.18602002055059	19.87517764790302
22	23.472150543133765	23.881612492773975	28.61677182182671	24.029465142265554
23	23.205116879534188	25.737829665688732	28.01699220596463	23.040061248812453
24	23.99831379787582	23.673316936257528	29.428721539527338	22.899647726339314
25	22.84137456469483	24.542609924725333	28.15492601943162	24.461089491148215
26	22.21927756777742	27.015809694732322	26.841610137050413	23.92330260043984
27	21.117666841796115	26.86857697249228	29.132551295105518	22.88120489060609
28	20.21551770899685	26.509174148413685	30.560708700833956	22.71459944175551
29	21.4505677758715	25.33999135201484	28.983923736549517	24.225517135564143
30	23.58590719379081	24.62583515824418	29.42624183052119	22.362015817443822
31	23.763361369543283	26.314671973243943	26.15209605152835	23.769870605684424
32	24.276506152003062	26.07801474496968	25.753327846977157	23.892151256050102
33	24.78391660738612	25.127356304737642	27.085861474156008	23.002865613720232
34	21.73402951163681	25.154633103805267	28.64528847539741	24.46604890916051
35	21.897690306042588	23.567619339870465	27.603190765563664	26.931499588523288
36	22.74265114988756	26.22168288551339	27.053470275263198	23.982195689335857
37	21.954878594996877	27.04448133011591	28.68992323750808	22.310716837379132
38	21.95999299482206	26.055387400288577	27.336777029215618	24.647842575673746
39	23.209456370294944	24.439702000970186	27.14676932661952	25.20407230211535
40	23.559250321974716	24.132838011459356	28.670860474523312	23.637051192042613
41	19.11514683751862	25.716287193697816	29.527909899773263	25.640656069010305
42	20.918515212239843	25.81904013564008	28.28759045126055	24.97485420085953
43	21.782848782695353	24.06139139571971	29.327673397526798	24.82808642405814
44	21.420036358733302	23.91539852798274	28.506579752866003	26.157985360417957
45	22.621920317650723	23.463316579799358	26.872141554188616	27.0426215483613
46	22.1682885513385	25.806641590609342	26.930104752207328	25.094965105844828
47	20.01543618856327	26.20695961328938	29.567120298432982	24.21048389971437
48	21.04838997143685	23.213795861055704	30.525682811122113	25.212131356385324
49	21.624767333553407	23.12886582759513	30.467564631290518	24.778802207560943
50	20.60607187746518	23.279973095157285	29.307680743664733	26.806274283712806
51	19.49314747914332	24.198705281935165	27.471611206424928	28.836536032496586
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	215.0
1	889.0
2	1563.0
3	3083.5
4	4604.0
5	3307.5
6	2011.0
7	1945.5
8	1880.0
9	1989.0
10	2098.0
11	2064.0
12	2030.0
13	2113.5
14	2197.0
15	1967.0
16	1737.0
17	1880.5
18	2024.0
19	2125.0
20	2226.0
21	2368.0
22	2510.0
23	2961.5
24	3413.0
25	3983.5
26	5714.5
27	6875.0
28	8600.0
29	10325.0
30	10350.5
31	10376.0
32	11798.5
33	13221.0
34	15383.0
35	17545.0
36	18074.5
37	18604.0
38	19856.0
39	21108.0
40	24715.5
41	28323.0
42	29994.5
43	31666.0
44	34202.0
45	36738.0
46	38832.0
47	40926.0
48	44342.5
49	47759.0
50	51962.0
51	56165.0
52	55311.5
53	54458.0
54	49787.0
55	45116.0
56	41060.5
57	37005.0
58	35809.5
59	34614.0
60	33169.0
61	31724.0
62	27478.0
63	23232.0
64	19522.5
65	15813.0
66	12986.0
67	10159.0
68	8632.5
69	7106.0
70	6128.0
71	5150.0
72	4479.0
73	3808.0
74	2899.5
75	1548.0
76	1105.0
77	841.5
78	578.0
79	485.5
80	393.0
81	309.0
82	225.0
83	121.5
84	18.0
85	19.0
86	20.0
87	12.0
88	4.0
89	10.5
90	17.0
91	12.0
92	7.0
93	4.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	645237.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.357848173647298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.51164302254638	20.84632011828729
2	10.67982953522509	6.057139689606948
3	4.072554314398253	3.4646663077991384
4	2.027175683940512	2.299453610659787
5	1.1597520356898203	1.6444036073585153
6	0.781602619577724	1.3298741050866063
7	0.5671044486524593	1.1257303297440484
8	0.42187488912831667	0.9570771243340074
9	0.36152932396310206	0.9226974311340298
>10	5.378371362336227	38.80879786548269
>50	0.9515108192913349	17.251336364359908
>100	0.0815423284627193	3.7452500474579375
>500	0.003305770072812945	0.578247483444238
>1k	0.00220384671520863	0.9690059152448429
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2252	0.34901904261534905	No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	1708	0.2647089364063127	No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	1122	0.17388959405613752	No Hit
TCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	1120	0.173579630430369	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	778	0.12057585042395275	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	687	0.10647250545148526	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.199272515370321E-4	0.0	0.0	0.1904726480347531	0.0
2	6.199272515370321E-4	0.0	0.0	0.8133445540165861	0.0
3	6.199272515370321E-4	0.0	0.0	1.2502382845373095	0.0
4	6.199272515370321E-4	0.0	0.0	1.785080520800884	0.0
5	6.199272515370321E-4	0.0	0.0	2.67157649049884	0.0
6	6.199272515370321E-4	0.0	0.0	3.276315524373215	0.0
7	6.199272515370321E-4	0.0	0.0	3.804338560869882	0.0
8	6.199272515370321E-4	0.0	0.0	4.601410024533621	0.0
9	6.199272515370321E-4	0.0	0.0	5.032414446164743	0.0
10	6.199272515370321E-4	0.0	0.0	5.831190709770208	0.0
11	6.199272515370321E-4	0.0	0.0	7.4355624367480475	0.0
12	6.199272515370321E-4	0.0	0.0	8.701763848012435	0.0
13	6.199272515370321E-4	0.0	0.0	9.100532052563631	0.0
14	6.199272515370321E-4	0.0	0.0	9.247764774803677	0.0
15	6.199272515370321E-4	0.0	0.0	9.48550687576813	0.0
16	6.199272515370321E-4	0.0	0.0	10.058784601627	0.0
17	6.199272515370321E-4	0.0	0.0	10.866084864941099	0.0
18	6.199272515370321E-4	0.0	0.0	11.81829312330198	0.0
19	6.199272515370321E-4	0.0	0.0	12.375297758808005	0.0
20	6.199272515370321E-4	0.0	0.0	12.99971948291868	0.0
21	6.199272515370321E-4	0.0	0.0	13.800975455530294	0.0
22	6.199272515370321E-4	0.0	0.0	14.618659500307638	0.0
23	7.749090644212902E-4	0.0	0.0	15.324291694369666	0.0
24	7.749090644212902E-4	0.0	0.0	15.889510365958555	0.0
25	7.749090644212902E-4	0.0	0.0	16.39025660338759	0.0
26	7.749090644212902E-4	0.0	0.0	16.81676655244507	0.0
27	7.749090644212902E-4	0.0	0.0	17.269158464254218	0.0
28	7.749090644212902E-4	0.0	0.0	17.72821459401739	0.0
29	7.749090644212902E-4	0.0	0.0	18.220281849924913	0.0
30	7.749090644212902E-4	0.0	0.0	18.771862121979986	0.0
31	7.749090644212902E-4	0.0	0.0	19.29864530397358	0.0
32	7.749090644212902E-4	0.0	0.0	19.815044704503926	0.0
33	7.749090644212902E-4	0.0	0.0	20.292543670000324	0.0
34	7.749090644212902E-4	0.0	0.0	20.782751144153234	0.0
35	9.298908773055481E-4	0.0	0.0	21.29868559924493	0.0
36	9.298908773055481E-4	0.0	0.0	21.79772703673224	0.0
37	9.298908773055481E-4	0.0	0.0	22.330089563989667	0.0
38	9.298908773055481E-4	0.0	0.0	22.824016601651795	0.0
39	9.298908773055481E-4	0.0	0.0	23.34615032925886	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACC	60	0.0	45.000004	24
ACCTACG	30	2.1640153E-6	45.000004	39
TCGACCC	60	0.0	45.000004	25
CGTCATA	35	1.2108285E-7	45.000004	38
ACGACTT	35	1.2108285E-7	45.000004	13
AACGTCG	20	7.0309837E-4	45.0	11
TTCACGT	20	7.0309837E-4	45.0	28
GTTAGCG	25	3.8888975E-5	45.0	1
TCTACCG	20	7.0309837E-4	45.0	1
CAGATAG	20	7.0309837E-4	45.0	11
GTCAATC	20	7.0309837E-4	45.0	10
TTAGTGT	45	3.8380676E-10	45.0	21
ACGTAGC	20	7.0309837E-4	45.0	22
AACTCGC	25	3.8888975E-5	45.0	38
GATTCGC	40	6.8066583E-9	45.0	11
TCGCGAT	20	7.0309837E-4	45.0	13
GTACGTA	20	7.0309837E-4	45.0	20
TACGTAG	20	7.0309837E-4	45.0	21
TAGCGGG	100	0.0	42.75	3
CGGGTAT	95	0.0	42.63158	6
>>END_MODULE