FastQCFastQC Report
Sat 18 Jun 2016
SRR3549701_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549701_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1227500
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC47420.38631364562118126No Hit
TCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC44800.364969450101833No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT43240.3522606924643584No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36670.29873727087576374No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC30150.2456211812627291No Hit
TCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG18430.15014256619144603No Hit
GCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG13850.11283095723014257No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG700.045.0000042
TCGCAAG207.033474E-445.01
TTATCCG207.033474E-445.01
ATCCGGT207.033474E-445.016
TGCGCAT207.033474E-445.013
CTACCGT207.033474E-445.019
TATAGCG207.033474E-445.01
ATACCGA207.033474E-445.010
CGACAAA302.1656233E-644.99999610
ACGGGTA302.1656233E-644.9999965
CACTCGA302.1656233E-644.99999644
TGCGACA302.1656233E-644.99999630
CGGTAGT501.0822987E-940.512
TTGGGTA8050.039.689445
CGAACTC403.458481E-739.37513
CGGTACA403.458481E-739.37520
TTGGGAT40650.039.188195
TTTGGGC26450.039.045374
CGTTTTT19250.038.9220771
CTTGGGA36150.038.9004144