##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549699_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	684195
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11861384546803	33.0	31.0	34.0	30.0	34.0
2	32.31006657458765	34.0	31.0	34.0	30.0	34.0
3	32.38394609723836	34.0	31.0	34.0	30.0	34.0
4	35.89333011787575	37.0	35.0	37.0	35.0	37.0
5	35.85791623733	37.0	35.0	37.0	35.0	37.0
6	35.471976556391084	37.0	35.0	37.0	33.0	37.0
7	35.83472255716572	37.0	35.0	37.0	35.0	37.0
8	35.90230124452824	37.0	35.0	37.0	35.0	37.0
9	37.64761361892443	39.0	38.0	39.0	35.0	39.0
10	37.18768479746271	39.0	37.0	39.0	34.0	39.0
11	37.0944979136065	39.0	37.0	39.0	33.0	39.0
12	36.837322693091885	39.0	35.0	39.0	33.0	39.0
13	36.685618865966575	39.0	35.0	39.0	33.0	39.0
14	37.80217920329731	40.0	36.0	41.0	33.0	41.0
15	37.854056226660525	40.0	36.0	41.0	33.0	41.0
16	37.92202807679097	40.0	36.0	41.0	33.0	41.0
17	37.83676144958674	40.0	36.0	41.0	33.0	41.0
18	37.8281586389845	39.0	36.0	41.0	33.0	41.0
19	37.84776416080211	40.0	36.0	41.0	33.0	41.0
20	37.79821980575713	40.0	36.0	41.0	33.0	41.0
21	37.640118679616194	39.0	36.0	41.0	33.0	41.0
22	37.709150169176915	39.0	36.0	41.0	33.0	41.0
23	37.669839738670994	39.0	36.0	41.0	33.0	41.0
24	37.61268936487405	39.0	35.0	41.0	33.0	41.0
25	37.53314186745007	39.0	35.0	41.0	33.0	41.0
26	37.44052061181388	39.0	35.0	41.0	33.0	41.0
27	37.37549967480031	39.0	35.0	41.0	33.0	41.0
28	37.23502656406434	39.0	35.0	41.0	32.0	41.0
29	37.134809520677585	39.0	35.0	41.0	32.0	41.0
30	37.12637040609768	39.0	35.0	41.0	32.0	41.0
31	37.024421400331775	39.0	35.0	41.0	32.0	41.0
32	36.891988395121274	39.0	35.0	41.0	31.0	41.0
33	36.85123100870366	39.0	35.0	41.0	31.0	41.0
34	36.666497124357825	39.0	35.0	41.0	31.0	41.0
35	36.62781955436681	39.0	35.0	41.0	31.0	41.0
36	36.479902659329575	39.0	35.0	40.0	30.0	41.0
37	36.45749091998626	39.0	35.0	40.0	30.0	41.0
38	36.38470465291328	39.0	35.0	40.0	30.0	41.0
39	36.28763145009829	38.0	35.0	40.0	30.0	41.0
40	36.136338324600445	38.0	35.0	40.0	30.0	41.0
41	36.070019511981236	38.0	35.0	40.0	30.0	41.0
42	36.0859652584424	38.0	35.0	40.0	30.0	41.0
43	36.03796724617982	38.0	35.0	40.0	30.0	41.0
44	35.843177749033536	38.0	35.0	40.0	29.0	41.0
45	35.868322627321156	38.0	35.0	40.0	29.0	41.0
46	35.73333041019008	38.0	35.0	40.0	29.0	41.0
47	35.529908871009	38.0	34.0	40.0	28.0	41.0
48	35.4641133010326	38.0	34.0	40.0	28.0	41.0
49	35.47268688020228	38.0	34.0	40.0	28.0	41.0
50	35.3654981401501	37.0	34.0	40.0	28.0	41.0
51	34.40619852527423	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	10.0
10	21.0
11	20.0
12	18.0
13	17.0
14	16.0
15	29.0
16	56.0
17	94.0
18	164.0
19	276.0
20	477.0
21	795.0
22	1186.0
23	1940.0
24	2748.0
25	4077.0
26	5566.0
27	6797.0
28	8258.0
29	9969.0
30	12225.0
31	15837.0
32	20828.0
33	29381.0
34	51355.0
35	61682.0
36	59661.0
37	86601.0
38	141736.0
39	162282.0
40	66.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.032483429431668	5.08787699413179	52.27851708942626	17.601122487010283
2	21.448271326157016	4.614912415320194	52.10488238002324	21.83193387849955
3	21.77142481310153	4.92345018598499	49.62664152763466	23.678483473278817
4	20.129641403401077	5.340290414282478	49.2806875232938	25.24938065902265
5	18.516066326120477	6.178940214412558	49.775867990850564	25.529125468616403
6	22.473271508853472	7.093007110545971	51.880969606618	18.552751773982564
7	82.80066355351909	2.6008667119753874	9.366774092181322	5.231695642324191
8	82.34626093438274	3.626305366160232	8.612749289310797	5.41468441014623
9	76.87267518762926	6.325535848698105	10.77032132652241	6.031467637150228
10	38.81393462390108	28.040982468448323	18.081394923961735	15.063687983688862
11	30.393820475156936	25.281242920512426	24.911903770124013	19.41303283420662
12	29.79092217861867	20.857796388456507	29.52783928558379	19.823442147341037
13	25.406499609029588	23.630251609555756	29.22982483064039	21.733423950774267
14	20.2928989542455	26.783007768253203	30.353627255387718	22.570466022113578
15	17.94546876256038	25.83152463844372	35.82998998823435	20.393016610761553
16	21.807671789475222	23.961589897616907	32.70412674749158	21.526611565416292
17	20.59135188067729	23.86117992677526	29.736844028383718	25.810624164163727
18	22.510541585366745	23.996813773851024	30.931971148576064	22.560673492206167
19	23.51040273606209	26.115508005758592	28.981649968210814	21.392439289968504
20	27.763430016296525	24.578226967458107	29.13350726035706	18.524835755888304
21	24.829763444632015	26.29908140223182	28.47287688451392	20.39827826862225
22	22.419485672944116	24.392315056380127	28.693135728849235	24.495063541826525
23	22.94287447292073	25.512609709220325	27.763868487784915	23.78064733007403
24	23.67102945797616	23.912334933754266	28.93064111839461	23.48599448987496
25	22.35561499280176	24.87404906495955	26.7008674427612	26.069468499477487
26	20.13110297502905	28.143730953894725	27.741798756202545	23.983367314873682
27	21.22450470991457	26.047544925057913	28.506639189120058	24.221311175907452
28	19.470180284860312	27.475792719911723	30.18028486031029	22.873742134917677
29	21.67773807174855	26.54316386410307	28.833300447971705	22.945797616176673
30	23.345391299264097	25.01289836961685	28.04814416942538	23.59356616169367
31	24.04153786566695	27.08906086715045	25.794985347744433	23.07441591943817
32	23.361614744334585	27.493185422284583	25.89378758979531	23.251412243585527
33	24.052645810039536	25.957950584263255	26.29791214492944	23.691491460767764
34	21.507464977089867	25.599280906759038	28.810061459086956	24.08319265706414
35	20.795825751430513	25.734914753834797	27.372167291488537	26.097092203246152
36	21.77347101338069	27.390144622512587	26.805515971323963	24.030868392782757
37	22.87213440612691	26.95386549156308	27.828323796578463	22.345676305731555
38	22.62030561462741	28.127069037335843	25.66183617243622	23.590789175600523
39	23.155971616278983	25.20246420976476	28.02768216663378	23.613882007322474
40	23.491548462061253	23.677606530302032	29.72295909791799	23.10788590971872
41	19.23705961019885	25.053530060874458	28.98398848281557	26.725421846111125
42	21.225820124379744	24.438500719824027	27.327004728184217	27.00867442761201
43	22.146610250001828	24.222918904698222	27.75012971448198	25.880341130817968
44	22.18446495516629	24.158463595904674	27.315019840834847	26.342051608094184
45	22.971375119666178	24.653497906298643	25.819247436768755	26.555879537266424
46	22.211504030283763	25.449469814891952	28.17383932943094	24.165186825393345
47	19.58169820007454	25.078522935712773	30.114660294214367	25.225118569998315
48	19.731947763430018	23.852264339844634	30.93679433494837	25.47899356177698
49	21.53552715234692	22.5334882599259	31.632210115537234	24.298774472189947
50	21.452363726715337	22.121617375163513	29.664788547124722	26.761230350996428
51	19.71835514728988	23.155240830464997	28.44393776628008	28.68246625596504
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	145.0
1	658.0
2	1171.0
3	2270.0
4	3369.0
5	2407.5
6	1446.0
7	1458.5
8	1471.0
9	1546.5
10	1622.0
11	1675.5
12	1729.0
13	1739.5
14	1750.0
15	1708.5
16	1667.0
17	1627.0
18	1587.0
19	1668.5
20	1750.0
21	2025.0
22	2300.0
23	2516.5
24	2733.0
25	3611.5
26	5261.5
27	6033.0
28	6956.0
29	7879.0
30	9189.5
31	10500.0
32	12768.5
33	15037.0
34	16245.5
35	17454.0
36	18396.5
37	19339.0
38	21661.0
39	23983.0
40	26577.0
41	29171.0
42	32710.0
43	36249.0
44	41140.5
45	46032.0
46	51346.0
47	56660.0
48	64188.5
49	71717.0
50	69885.5
51	68054.0
52	59211.5
53	50369.0
54	44633.0
55	38897.0
56	35639.5
57	32382.0
58	31025.5
59	29669.0
60	28887.5
61	28106.0
62	25180.0
63	22254.0
64	18987.0
65	15720.0
66	12890.0
67	10060.0
68	8266.0
69	6472.0
70	5704.0
71	4936.0
72	4372.5
73	3809.0
74	3393.5
75	2298.0
76	1618.0
77	1180.0
78	742.0
79	614.0
80	486.0
81	330.5
82	175.0
83	133.5
84	92.0
85	54.5
86	17.0
87	43.5
88	70.0
89	35.5
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	684195.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.986172147528066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.36066065006007	18.987649007163252
2	11.99465659543398	6.473797354697286
3	5.068136692334821	4.1030882773965285
4	2.6975939335928882	2.9119093710426034
5	1.5656531557916864	2.1125492792757514
6	1.007622433007244	1.6315123462106729
7	0.7025895087282199	1.3272141002110842
8	0.5235971163498111	1.1303905534212229
9	0.3840777181560761	0.9328308678170671
>10	4.237657986260366	28.378956399271026
>50	1.3226959619951377	23.94497496400006
>100	0.12904350848732624	5.657261145622681
>500	0.0032807671649320233	0.6437995351561532
>1k	0.0027339726374433528	1.7640667987146026
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	2861	0.4181556427626627	No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	2711	0.3962320683430893	No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	2347	0.34303086108492464	No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	2311	0.337769203224227	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1725	0.2521211058250937	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	961	0.1404570334480667	No Hit
TCCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG	929	0.1357800042385577	No Hit
GCCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG	762	0.1113717580514327	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4615716279715578E-4	0.0	0.0	0.42765585834447783	0.0
2	1.4615716279715578E-4	0.0	0.0	1.6629761983060385	0.0
3	1.4615716279715578E-4	0.0	0.0	2.355761149964557	0.0
4	1.4615716279715578E-4	0.0	0.0	3.145009829069198	0.0
5	1.4615716279715578E-4	0.0	0.0	4.350806422145733	0.0
6	1.4615716279715578E-4	0.0	0.0	5.134354971901286	0.0
7	1.4615716279715578E-4	0.0	0.0	5.852717427049306	0.0
8	1.4615716279715578E-4	0.0	0.0	6.930188031189939	0.0
9	1.4615716279715578E-4	0.0	0.0	7.484269835353956	0.0
10	1.4615716279715578E-4	0.0	0.0	8.508977703724815	0.0
11	1.4615716279715578E-4	0.0	0.0	10.38753571715666	0.0
12	1.4615716279715578E-4	0.0	0.0	12.066443046207587	0.0
13	1.4615716279715578E-4	0.0	0.0	12.613509306557342	0.0
14	1.4615716279715578E-4	0.0	0.0	12.81710623433378	0.0
15	1.4615716279715578E-4	0.0	0.0	13.158090895139544	0.0
16	1.4615716279715578E-4	0.0	0.0	13.908754083265736	0.0
17	1.4615716279715578E-4	0.0	0.0	14.983740015638816	0.0
18	1.4615716279715578E-4	0.0	0.0	16.26773069081183	0.0
19	1.4615716279715578E-4	0.0	0.0	17.005970520100263	0.0
20	1.4615716279715578E-4	0.0	0.0	17.834681633160137	0.0
21	1.4615716279715578E-4	0.0	0.0	18.87152054604316	0.0
22	1.4615716279715578E-4	0.0	0.0	19.88599741301822	0.0
23	1.4615716279715578E-4	0.0	0.0	20.80927221040785	0.0
24	1.4615716279715578E-4	0.0	0.0	21.578497358209283	0.0
25	1.4615716279715578E-4	0.0	0.0	22.24351244893634	0.0
26	1.4615716279715578E-4	0.0	0.0	22.805194425565812	0.0
27	1.4615716279715578E-4	0.0	0.0	23.353283786055144	0.0
28	1.4615716279715578E-4	0.0	0.0	23.942735623616073	0.0
29	1.4615716279715578E-4	0.0	0.0	24.556157235875737	0.0
30	1.4615716279715578E-4	0.0	0.0	25.229357127719435	0.0
31	1.4615716279715578E-4	0.0	0.0	25.90124160509796	0.0
32	1.4615716279715578E-4	0.0	0.0	26.534248277172445	0.0
33	1.4615716279715578E-4	0.0	0.0	27.136708102222318	0.0
34	1.4615716279715578E-4	0.0	0.0	27.726013782620452	0.0
35	1.4615716279715578E-4	0.0	0.0	28.348789453299133	0.0
36	1.4615716279715578E-4	0.0	0.0	28.957095564860897	0.0
37	1.4615716279715578E-4	0.0	0.0	29.54830128837539	0.0
38	2.9231432559431156E-4	0.0	0.0	30.16391525807701	0.0
39	2.9231432559431156E-4	0.0	0.0	30.779236913453037	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCCG	20	7.031282E-4	45.000004	4
ACGCCTA	20	7.031282E-4	45.000004	28
CAACGTG	25	3.8891434E-5	45.000004	37
TTTCGAC	50	2.1827873E-11	45.000004	24
CGACCTA	20	7.031282E-4	45.000004	16
GTTAGTC	20	7.031282E-4	45.000004	16
CAATTCG	20	7.031282E-4	45.000004	37
AAGCGGT	25	3.8891434E-5	45.000004	23
TCTAGCG	20	7.031282E-4	45.000004	1
ATTCCGG	25	3.8891434E-5	45.000004	2
GTCACGA	20	7.031282E-4	45.000004	8
TGTTCCG	40	6.8084773E-9	45.000004	1
AAACCGG	20	7.031282E-4	45.000004	26
AACGATC	20	7.031282E-4	45.000004	23
GTTCCGC	20	7.031282E-4	45.000004	26
ACTCGTT	25	3.8891434E-5	45.000004	11
ACTCGAT	20	7.031282E-4	45.000004	34
TACTCGA	20	7.031282E-4	45.000004	33
CTATTCG	20	7.031282E-4	45.000004	11
TGGATCG	20	7.031282E-4	45.000004	27
>>END_MODULE