##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549697_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	781552
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16146334472946	33.0	31.0	34.0	30.0	34.0
2	32.36240198988679	34.0	31.0	34.0	30.0	34.0
3	32.42417011280119	34.0	31.0	34.0	30.0	34.0
4	35.91255604233628	37.0	35.0	37.0	35.0	37.0
5	35.87480807419084	37.0	35.0	37.0	35.0	37.0
6	35.46670471062706	37.0	35.0	37.0	33.0	37.0
7	35.81189095543227	37.0	35.0	37.0	35.0	37.0
8	35.88781424652486	37.0	35.0	37.0	35.0	37.0
9	37.67342288165087	39.0	38.0	39.0	35.0	39.0
10	37.20668362437816	39.0	37.0	39.0	34.0	39.0
11	37.13069251949966	39.0	37.0	39.0	34.0	39.0
12	36.914746555571476	39.0	35.0	39.0	33.0	39.0
13	36.77136390157021	39.0	35.0	39.0	33.0	39.0
14	37.903936014494235	40.0	37.0	41.0	33.0	41.0
15	37.95058678117387	40.0	37.0	41.0	33.0	41.0
16	38.02996729584212	40.0	37.0	41.0	33.0	41.0
17	37.99436505824308	40.0	37.0	41.0	33.0	41.0
18	37.94506315638627	40.0	36.0	41.0	33.0	41.0
19	37.964429750035826	40.0	37.0	41.0	34.0	41.0
20	37.88662558601347	40.0	36.0	41.0	33.0	41.0
21	37.72504068827154	39.0	36.0	41.0	33.0	41.0
22	37.8047385202776	39.0	36.0	41.0	33.0	41.0
23	37.77324733351076	39.0	36.0	41.0	33.0	41.0
24	37.7195720822159	39.0	36.0	41.0	33.0	41.0
25	37.63852181300796	39.0	36.0	41.0	33.0	41.0
26	37.5769046717301	39.0	35.0	41.0	33.0	41.0
27	37.513482148340735	39.0	35.0	41.0	33.0	41.0
28	37.377143171535614	39.0	35.0	41.0	32.0	41.0
29	37.284651565090996	39.0	35.0	41.0	32.0	41.0
30	37.27448333572174	39.0	35.0	41.0	32.0	41.0
31	37.20015686736135	39.0	35.0	41.0	32.0	41.0
32	37.06558616701128	39.0	35.0	41.0	32.0	41.0
33	37.00167999058284	39.0	35.0	41.0	31.0	41.0
34	36.8461689049481	39.0	35.0	41.0	31.0	41.0
35	36.772411816488216	39.0	35.0	41.0	31.0	41.0
36	36.59825961676255	39.0	35.0	40.0	31.0	41.0
37	36.5475950416607	39.0	35.0	40.0	30.0	41.0
38	36.45605538722951	39.0	35.0	40.0	30.0	41.0
39	36.38947632403218	39.0	35.0	40.0	30.0	41.0
40	36.27171704505906	38.0	35.0	40.0	30.0	41.0
41	36.20142613671259	38.0	35.0	40.0	30.0	41.0
42	36.158796855487545	38.0	35.0	40.0	30.0	41.0
43	36.088343449955985	38.0	35.0	40.0	30.0	41.0
44	35.927476354740314	38.0	35.0	40.0	30.0	41.0
45	35.988888775155075	38.0	35.0	40.0	30.0	41.0
46	35.890580537187546	38.0	35.0	40.0	30.0	41.0
47	35.71383989804901	38.0	35.0	40.0	29.0	41.0
48	35.609848097119574	38.0	34.0	40.0	29.0	41.0
49	35.595370493582	38.0	34.0	40.0	29.0	41.0
50	35.48903975679161	37.0	34.0	40.0	29.0	41.0
51	34.49403110733515	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	16.0
10	25.0
11	25.0
12	26.0
13	19.0
14	27.0
15	45.0
16	44.0
17	100.0
18	153.0
19	292.0
20	495.0
21	806.0
22	1333.0
23	1952.0
24	2975.0
25	4227.0
26	5804.0
27	7387.0
28	8919.0
29	10732.0
30	13356.0
31	17252.0
32	22984.0
33	32113.0
34	55025.0
35	69671.0
36	70293.0
37	101519.0
38	163112.0
39	190747.0
40	75.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.067112616946794	4.783942719102504	53.884450426843	17.2644942371077
2	20.00877740700555	4.8896298646795096	53.44596392818393	21.655628800131023
3	20.54348271132311	5.632638647204537	50.81517288676889	23.008705754703463
4	19.333710360922883	5.004913300714476	51.02066145310869	24.640714885253956
5	18.88089340184658	6.263434806641145	49.115222019776034	25.74044977173624
6	21.367995987471083	7.490224578786823	52.094294429545315	19.047485004196776
7	80.59885970479252	3.0677421336008353	10.894604581652917	5.4387935799537335
8	81.8292320920425	4.3019530370340044	8.404942985239625	5.46387188568387
9	76.56560280058142	5.544480725530739	10.938235715601778	6.951680758286077
10	40.13872397486028	23.99981063320163	19.09316334677667	16.768302045161423
11	29.5262503326714	25.35519069748398	25.11707474358712	20.0014842262575
12	27.065121706553114	21.332041885888593	30.51991422195836	21.082922185599934
13	24.531956926730402	23.00205232665261	30.838766966241533	21.627223780375456
14	19.164047945626137	26.191475423260385	30.55190185681823	24.092574774295247
15	17.26871660490921	24.433946813519768	36.098813642598316	22.19852293897271
16	19.33166315229185	23.45410158249227	32.874076197105246	24.34015906811063
17	19.983571150735973	23.19730484983725	30.087441398652935	26.731682600773844
18	20.466200585501667	23.978698794194116	31.617729850349047	23.937370769955166
19	23.361337441398653	25.165312096955798	28.777739676950475	22.69561078469507
20	26.233571150735973	24.549486150633612	29.208677093782626	20.008265604847793
21	24.12648166724671	26.172794644502222	28.220643028231006	21.48008066002006
22	22.53324155014638	23.573095584170982	28.119050299916065	25.774612565766574
23	22.428578008884887	24.458513317092144	28.101393125473418	25.01151554854955
24	22.79451655168178	23.15456936966446	29.5755112903556	24.475402788298155
25	21.38130284357279	25.100185272381108	27.94989968677708	25.568612197269026
26	19.69977685425922	26.351029745941407	29.388831453313408	24.56036194648597
27	20.68064568960223	25.806984052244765	30.66296292505169	22.849407333101315
28	19.71871353409626	24.952786250946836	32.064277232992815	23.264222981964092
29	20.2390883779966	23.621844739697423	31.565909881876063	24.573157000429912
30	21.620954203942926	25.148422625749788	30.24405285892685	22.986570311380433
31	22.787735173091487	26.48998403177268	27.22326857330031	23.499012221835528
32	22.878835957172395	26.446864699981575	27.001402337912257	23.672897004933773
33	23.0289219399349	26.395812434745224	26.685492456036197	23.889773169283682
34	21.329610825639243	24.781844330255694	29.355564313059148	24.53298053104592
35	21.141267631584334	24.00723176448912	30.169329743894203	24.682170860032347
36	23.144461277048745	25.579999795279136	29.543779556574613	21.73175937109751
37	21.883380760333285	25.901923352508856	30.73973324871538	21.47496263844248
38	22.57648883247692	25.715883268163857	28.624582881241427	23.083045018117794
39	21.29378467459619	24.781332528097938	30.352938767989844	23.57194402931603
40	21.300310152107603	24.953042152025713	30.15487133293754	23.591776362929146
41	19.882746125657665	24.998976395684483	28.972480397977357	26.145797080680495
42	22.506499887403525	23.972429217761583	28.826616783016355	24.694454111818537
43	23.759263619055417	22.194428521710645	29.946951706348397	24.09935615288554
44	21.681986611255553	23.01906974839806	29.81132413454255	25.48761950580384
45	21.27651135177186	22.942811226892132	28.893406964603763	26.887270456732242
46	21.713718345036543	24.927835895756136	30.17406391385346	23.18438184535386
47	19.720760742727293	24.339519315413433	32.089995291420145	23.84972465043913
48	20.41425266648924	24.002625545069296	31.53008373083301	24.05303805760845
49	21.900526132618175	22.466707269637848	32.283200605973754	23.34956599177022
50	21.2620529408152	22.133907916555774	30.175855221405612	26.428183921223415
51	19.403059553299077	23.147020287837535	28.267089074047536	29.182831084815852
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	279.0
1	851.5
2	1424.0
3	2853.0
4	4282.0
5	2895.0
6	1508.0
7	1509.0
8	1510.0
9	1698.0
10	1886.0
11	1919.5
12	1953.0
13	1814.0
14	1675.0
15	1597.0
16	1519.0
17	1702.5
18	1886.0
19	1853.0
20	1820.0
21	1877.0
22	1934.0
23	2309.5
24	2685.0
25	3242.0
26	4600.5
27	5402.0
28	6891.0
29	8380.0
30	9838.5
31	11297.0
32	12639.5
33	13982.0
34	16892.0
35	19802.0
36	21834.5
37	23867.0
38	26723.0
39	29579.0
40	34879.5
41	40180.0
42	45497.5
43	50815.0
44	62318.5
45	73822.0
46	79988.5
47	86155.0
48	83033.0
49	79911.0
50	68509.5
51	57108.0
52	51486.5
53	45865.0
54	41239.5
55	36614.0
56	35632.5
57	34651.0
58	33613.5
59	32576.0
60	30243.5
61	27911.0
62	25353.5
63	22796.0
64	20413.5
65	18031.0
66	14978.0
67	11925.0
68	9757.5
69	7590.0
70	6692.5
71	5795.0
72	4850.0
73	3905.0
74	3278.0
75	1974.5
76	1298.0
77	1008.5
78	719.0
79	563.5
80	408.0
81	300.5
82	193.0
83	121.5
84	50.0
85	53.0
86	56.0
87	48.0
88	40.0
89	24.0
90	8.0
91	8.0
92	8.0
93	4.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	781552.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.974937988599144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.93907750301537	18.86602278623669
2	12.029001706430535	6.489631501914339
3	5.152458944951385	4.169617815865997
4	2.7437947906478986	2.960547773246738
5	1.6177606072432598	2.1819496030392713
6	1.0748179119612329	1.7395887914513921
7	0.7127911621413283	1.3459248178308698
8	0.5750871151307704	1.2410351414955927
9	0.39329452397661835	0.954818585597241
>10	4.391220789646939	29.51346068743876
>50	1.2481319319744715	22.190070802184554
>100	0.11490282457516678	5.321835606620727
>500	0.003351332383442365	0.5495149123027372
>1k	0.004308855921568754	2.4759811747751064
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT	3597	0.460238090363789	No Hit
TCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	2997	0.3834677667001044	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	2801	0.3583894609699675	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	2525	0.32307511208467254	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2515	0.3217956066902778	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGC	1251	0.16006612483878233	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCG	1239	0.15853071836550864	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC	1180	0.15098163653857966	No Hit
TCCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG	1067	0.13652322558191907	No Hit
GCCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG	794	0.10159272831494258	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2795053943947428E-4	0.0	0.0	0.49299342846029437	0.0
2	1.2795053943947428E-4	0.0	0.0	1.6805023849980552	0.0
3	1.2795053943947428E-4	0.0	0.0	2.3692601388007453	0.0
4	1.2795053943947428E-4	0.0	0.0	3.2098951829180913	0.0
5	1.2795053943947428E-4	0.0	0.0	4.568729911765308	0.0
6	1.2795053943947428E-4	0.0	0.0	5.367269228407067	0.0
7	1.2795053943947428E-4	0.0	0.0	6.127935185374741	0.0
8	1.2795053943947428E-4	0.0	0.0	7.357795770466968	0.0
9	1.2795053943947428E-4	0.0	0.0	7.962234118779045	0.0
10	1.2795053943947428E-4	0.0	0.0	9.2628513521813	0.0
11	1.2795053943947428E-4	0.0	0.0	11.23930333490286	0.0
12	1.2795053943947428E-4	0.0	0.0	13.170716727741723	0.0
13	1.2795053943947428E-4	0.0	0.0	13.753915286506848	0.0
14	2.5590107887894857E-4	0.0	0.0	13.965417528200298	0.0
15	2.5590107887894857E-4	0.0	0.0	14.316129956803898	0.0
16	2.5590107887894857E-4	0.0	0.0	15.127464327389605	0.0
17	2.5590107887894857E-4	0.0	0.0	16.149405285892687	0.0
18	2.5590107887894857E-4	0.0	0.0	17.47011075398694	0.0
19	2.5590107887894857E-4	0.0	0.0	18.28298053104592	0.0
20	2.5590107887894857E-4	0.0	0.0	19.152404446537147	0.0
21	2.5590107887894857E-4	0.0	0.0	20.27657788605237	0.0
22	2.5590107887894857E-4	0.0	0.0	21.359679202407516	0.0
23	2.5590107887894857E-4	0.0	0.0	22.379189100661247	0.0
24	2.5590107887894857E-4	0.0	0.0	23.177216615145248	0.0
25	2.5590107887894857E-4	0.0	0.0	23.883887444469465	0.0
26	2.5590107887894857E-4	0.0	0.0	24.514299752287755	0.0
27	2.5590107887894857E-4	0.0	0.0	25.08278399901734	0.0
28	2.5590107887894857E-4	0.0	0.0	25.7153714660061	0.0
29	2.5590107887894857E-4	0.0	0.0	26.392869572338117	0.0
30	3.838516183184228E-4	0.0	0.0	27.115534219092268	0.0
31	3.838516183184228E-4	0.0	0.0	27.797766495383545	0.0
32	3.838516183184228E-4	0.0	0.0	28.433296824779415	0.0
33	3.838516183184228E-4	0.0	0.0	29.10657256330993	0.0
34	3.838516183184228E-4	0.0	0.0	29.749779925072165	0.0
35	3.838516183184228E-4	0.0	0.0	30.435850717546625	0.0
36	3.838516183184228E-4	0.0	0.0	31.061528855405655	0.0
37	3.838516183184228E-4	0.0	0.0	31.715228161401928	0.0
38	3.838516183184228E-4	0.0	0.0	32.3319497615002	0.0
39	3.838516183184228E-4	0.0	0.0	33.02288267447336	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCATA	35	1.211265E-7	45.000004	6
GGGCGTA	70	0.0	45.000004	7
GTTGCGT	35	1.211265E-7	45.000004	1
TAATACG	20	7.0318993E-4	45.0	22
ACGTTTA	20	7.0318993E-4	45.0	24
AACCCGA	25	3.8896556E-5	45.0	24
TAGAACG	25	3.8896556E-5	45.0	11
TAGCGGT	20	7.0318993E-4	45.0	5
ATTCGGT	20	7.0318993E-4	45.0	40
TACGGCA	20	7.0318993E-4	45.0	13
TAGCCGT	40	6.8102963E-9	45.0	44
TACGCTT	20	7.0318993E-4	45.0	14
CGCTAGC	25	3.8896556E-5	45.0	14
TGCGAAG	20	7.0318993E-4	45.0	14
ACTACGC	20	7.0318993E-4	45.0	12
CGAATTC	20	7.0318993E-4	45.0	24
ATGACGG	20	7.0318993E-4	45.0	27
TTCGGTA	20	7.0318993E-4	45.0	25
GCAATCA	20	7.0318993E-4	45.0	9
GGCGATA	55	1.8189894E-12	45.0	8
>>END_MODULE