FastQCFastQC Report
Sat 18 Jun 2016
SRR3549694_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549694_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144244
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGC98930.8645883220711666No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCG92110.8049856499138296No Hit
GAATCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTC63560.5554759299589948No Hit
CCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC35550.31068548316617783Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC32450.2835933594582974Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGCT28230.246713113636602Illumina Single End Adapter 1 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCT22420.19593723017118725No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTC19790.17295262199321124No Hit
TCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC19460.17006862172753365Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18350.16036789356116354No Hit
GAATCAGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTC16620.14524874065321733No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATATCGCTCGTA16330.14271431617731883No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATATCGCT14690.1283817087963756No Hit
GAACTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCT12900.11273819220376073No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGCG750.045.036
TATTGCG253.8907954E-545.01
TCGTGCG551.8189894E-1245.01
TGCGATC207.0332753E-445.014
GCGTATC207.0332753E-445.022
TCGCGAG207.0332753E-445.01
TAGGGCG3950.043.291145
AGGGCGA5650.042.212396
TTTAGGG44250.041.8983083
TAGGGTG8950.041.4804465
CGTTTAT5750.041.0869561
TTTTAGG27250.040.376152
CGTTTTT15050.040.3654481
TACGGCT12000.040.31257
TTAGGGA20100.040.1865654
TTAGGGT15600.040.0961534
TCGTTAG1350.040.0000041
CGAGGGT2250.040.04
CGTTTGG6150.039.8780482
TTGAGGG18400.039.8641323
Produced by FastQC (version 0.11.5)