##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549694_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1144244
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37879945186516	33.0	31.0	34.0	31.0	34.0
2	32.546991725541055	34.0	31.0	34.0	31.0	34.0
3	32.59394761956366	34.0	31.0	34.0	31.0	34.0
4	36.06249278999933	37.0	35.0	37.0	35.0	37.0
5	35.268377199268684	37.0	35.0	37.0	35.0	37.0
6	26.74979375028403	37.0	17.0	37.0	0.0	37.0
7	31.4582973561583	37.0	25.0	37.0	17.0	37.0
8	34.80394828375766	37.0	32.0	37.0	32.0	37.0
9	37.355660156400205	39.0	37.0	39.0	35.0	39.0
10	37.30840712295629	39.0	37.0	39.0	34.0	39.0
11	37.368946658230236	39.0	37.0	39.0	34.0	39.0
12	37.209162556238006	39.0	37.0	39.0	34.0	39.0
13	37.24870744351729	39.0	37.0	39.0	34.0	39.0
14	38.523574517323226	40.0	38.0	41.0	34.0	41.0
15	38.60647903768776	40.0	38.0	41.0	35.0	41.0
16	38.71077497456836	40.0	38.0	41.0	35.0	41.0
17	38.64816857243735	40.0	38.0	41.0	35.0	41.0
18	38.62806009906978	40.0	38.0	41.0	35.0	41.0
19	38.63547547551047	40.0	38.0	41.0	35.0	41.0
20	38.55613488032273	40.0	38.0	41.0	34.0	41.0
21	38.50499456409647	40.0	38.0	41.0	34.0	41.0
22	38.50754996311976	40.0	38.0	41.0	34.0	41.0
23	38.43182135977991	40.0	38.0	41.0	34.0	41.0
24	38.36762613568435	40.0	37.0	41.0	34.0	41.0
25	38.284168411632486	40.0	37.0	41.0	34.0	41.0
26	38.243158801794024	40.0	37.0	41.0	34.0	41.0
27	38.191435567938306	40.0	37.0	41.0	34.0	41.0
28	38.14175822639227	40.0	37.0	41.0	34.0	41.0
29	38.126533326807916	40.0	37.0	41.0	34.0	41.0
30	37.886977777467045	40.0	37.0	41.0	33.0	41.0
31	37.95072816637011	40.0	37.0	41.0	34.0	41.0
32	37.872606716749225	40.0	37.0	41.0	33.0	41.0
33	37.802249345419334	40.0	37.0	41.0	33.0	41.0
34	37.74134450344507	40.0	37.0	41.0	33.0	41.0
35	37.71241798078032	40.0	37.0	41.0	33.0	41.0
36	37.65788328363531	40.0	37.0	41.0	33.0	41.0
37	37.55701231555508	40.0	37.0	41.0	33.0	41.0
38	37.50137470679331	40.0	36.0	41.0	33.0	41.0
39	37.46344573360227	40.0	36.0	41.0	33.0	41.0
40	37.39917360283296	40.0	36.0	41.0	33.0	41.0
41	37.321702364181064	40.0	36.0	41.0	33.0	41.0
42	37.31219040694118	40.0	36.0	41.0	33.0	41.0
43	37.2448935716508	40.0	36.0	41.0	32.0	41.0
44	37.105771146713465	40.0	36.0	41.0	32.0	41.0
45	37.07452344080458	40.0	35.0	41.0	32.0	41.0
46	36.97611436022387	39.0	35.0	41.0	31.0	41.0
47	36.84283509461269	39.0	35.0	41.0	31.0	41.0
48	36.817998608688356	39.0	35.0	41.0	31.0	41.0
49	36.82391605286984	39.0	35.0	41.0	31.0	41.0
50	36.726361685095135	39.0	35.0	41.0	31.0	41.0
51	35.6842535333373	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	4.0
11	12.0
12	2.0
13	4.0
14	2.0
15	13.0
16	37.0
17	60.0
18	145.0
19	360.0
20	619.0
21	1119.0
22	1745.0
23	2505.0
24	3720.0
25	5579.0
26	7163.0
27	8163.0
28	9394.0
29	11247.0
30	14318.0
31	19207.0
32	26362.0
33	40980.0
34	62446.0
35	84115.0
36	99289.0
37	172470.0
38	261429.0
39	311536.0
40	194.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.23806111283957	3.8640359923233154	53.8911281160312	17.00677477880592
2	19.264160441304476	7.833294297370141	52.02378164097867	20.87876362034671
3	18.577506196230875	8.0246870422742	52.882427174623594	20.515379586871333
4	17.51191179503672	4.271029605573636	55.367648858110684	22.849409741278958
5	19.792456853608144	7.0767248943407175	50.34983797162143	22.7809802804297
6	15.076592055540603	31.39400337690213	41.207294947581104	12.322109619976159
7	83.71597316656238	1.428366677037415	11.078231565994665	3.7774285904055427
8	85.12983244832397	4.105243287270897	6.788674443562737	3.9762498208424075
9	80.64407591387851	3.415268072194392	8.64046479597009	7.30019121795701
10	39.38670423441154	20.7116663928323	22.375384970338494	17.526244402417664
11	29.60234006033678	26.77584501207784	23.084324672010514	20.537490255574863
12	24.539608684860923	22.50062049702686	30.221526177983016	22.738244640129203
13	22.88366816867731	23.756646309703175	33.514442723754726	19.84524279786479
14	20.60810456511024	26.744994948629834	30.8273410216702	21.819559464589723
15	18.45646557901986	26.070663250145948	34.768108899850034	20.704762270984162
16	19.324200083199038	24.874851867259082	31.78998535277441	24.010962696767475
17	19.90196146975645	25.763823100667338	31.079472560048384	23.254742869527828
18	19.876005467365353	25.84204068363041	31.89013881654612	22.391815032458112
19	21.791855583249724	27.489241805069547	29.037250796158858	21.681651815521864
20	23.51264240843736	26.22736059791443	31.19247293409448	19.06752405955373
21	22.711414698263656	28.01010973184041	29.320756761669713	19.95771880822622
22	21.540335802503662	24.617826267823993	29.450624167572652	24.391213762099692
23	20.64017814382247	27.55636035670714	28.966286910833706	22.83717458863669
24	20.91809089669686	25.23281747599288	29.757376923103813	24.091714704206446
25	20.339280782770107	29.026326552728264	27.366715490751975	23.267677173749654
26	20.489510978427678	27.394244584197075	29.269893484256855	22.846350953118392
27	21.186128133509985	28.148104774855714	28.898469207616557	21.767297884017744
28	18.466778064818342	26.7029584599089	32.44325510992411	22.38700836534865
29	21.454252764270556	25.636490119240303	30.097077196821658	22.812179919667482
30	20.126214338899747	27.722671038694546	30.625461003072772	21.525653619332942
31	20.608017171162793	27.306064091225295	28.672381065576925	23.413537672034987
32	21.986744086051576	28.658922397670427	27.669972488385348	21.684361027892653
33	20.212384771080295	26.465421710754	28.520402990970457	24.80179052719525
34	21.274133838586877	25.423248887475047	28.59241560366495	24.710201670273126
35	21.33662051100989	26.46323686206788	27.834535291423855	24.365607335498375
36	21.99670699606028	26.789740649721562	28.142511562219248	23.07104079199891
37	18.93101471364499	29.56371193556619	29.846081779760258	21.659191571028558
38	19.919877228982628	27.757628617672452	29.454556895207666	22.86793725813725
39	20.31865581117314	26.681284760942596	29.42991180202824	23.570147625856023
40	21.790369886143164	24.090403794994774	30.66024379415579	23.45898252470627
41	20.09265506308095	25.370200761376072	27.610894179912677	26.926249995630304
42	21.763539944277618	25.154774680924696	28.616011969475043	24.46567340532264
43	21.051628848392475	25.627925512390714	28.269057998119283	25.051387641097527
44	20.406224546512806	25.551281020481646	29.66010745959778	24.38238697340777
45	20.676184450169718	24.794362041662442	28.358811582145066	26.17064192602277
46	22.7850877959596	26.232342052918785	28.02016003579656	22.962410115325053
47	19.755926183576232	25.263230569703666	31.826690810701212	23.15415243601889
48	20.198489133436574	25.588423448145676	29.750385407308233	24.46270201110952
49	20.794690642904833	23.50232992263888	32.1012825935727	23.601696840883587
50	20.56711680375864	23.498571982898753	29.405878466480928	26.528432746861686
51	19.50982482757174	23.77281418998046	27.820377471937803	28.896983510509994
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	159.0
1	617.5
2	1076.0
3	3049.0
4	5022.0
5	3586.5
6	2151.0
7	2103.0
8	2055.0
9	2082.0
10	2109.0
11	2228.5
12	2348.0
13	2375.5
14	2403.0
15	2383.0
16	2363.0
17	2419.0
18	2475.0
19	2455.0
20	2435.0
21	2737.0
22	3039.0
23	4112.5
24	5186.0
25	6350.0
26	9505.5
27	11497.0
28	13169.0
29	14841.0
30	17865.0
31	20889.0
32	24107.5
33	27326.0
34	30690.5
35	34055.0
36	37734.0
37	41413.0
38	46183.0
39	50953.0
40	56920.5
41	62888.0
42	69519.5
43	76151.0
44	83905.5
45	91660.0
46	116397.5
47	141135.0
48	133258.0
49	125381.0
50	118080.0
51	110779.0
52	94217.0
53	77655.0
54	66803.5
55	55952.0
56	50079.5
57	44207.0
58	39810.5
59	35414.0
60	31750.0
61	28086.0
62	23625.0
63	19164.0
64	16055.0
65	12946.0
66	10455.0
67	7964.0
68	6710.5
69	5457.0
70	4316.0
71	3175.0
72	2747.5
73	2320.0
74	1818.5
75	985.0
76	653.0
77	443.0
78	233.0
79	211.0
80	189.0
81	161.5
82	134.0
83	93.0
84	52.0
85	32.0
86	12.0
87	10.0
88	8.0
89	5.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1144244.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.60359583084451
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.16848421357082	24.626854636202424
2	10.992542678400536	7.607630079933624
3	4.089033353209255	4.2448577247988775
4	2.2860077685247218	3.164163555528009
5	1.5495490369672036	2.6809984297643727
6	1.177554256186633	2.4448566929983793
7	0.9869567817330208	2.3906577504320334
8	0.8089198191583568	2.2393227585372486
9	0.6871844576456086	2.140114791024606
>10	6.121287445406097	39.19835910308416
>50	0.093333796129353	2.2390459769482383
>100	0.032924449824857704	1.9709965298531744
>500	0.002592476351521648	0.6439312297722383
>1k	0.0028517239866738125	2.124219502615886
>5k	7.777429054564943E-4	2.283991238506713
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGC	9893	0.8645883220711666	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCG	9211	0.8049856499138296	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTC	6356	0.5554759299589948	No Hit
CCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC	3555	0.31068548316617783	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC	3245	0.2835933594582974	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGCT	2823	0.246713113636602	Illumina Single End Adapter 1 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCT	2242	0.19593723017118725	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTC	1979	0.17295262199321124	No Hit
TCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC	1946	0.17006862172753365	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1835	0.16036789356116354	No Hit
GAATCAGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTC	1662	0.14524874065321733	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATATCGCTCGTA	1633	0.14271431617731883	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATATCGCT	1469	0.1283817087963756	No Hit
GAACTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCT	1290	0.11273819220376073	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.739394744477576E-5	0.0	0.0	0.25947262996353926	0.0
2	8.739394744477576E-5	0.0	0.0	1.0785287054159778	0.0
3	8.739394744477576E-5	0.0	0.0	1.4656838925963342	0.0
4	8.739394744477576E-5	0.0	0.0	1.970995696722028	0.0
5	8.739394744477576E-5	0.0	0.0	3.2073578712232704	0.0
6	8.739394744477576E-5	0.0	0.0	3.725866161413125	0.0
7	8.739394744477576E-5	0.0	0.0	4.470113017852835	0.0
8	8.739394744477576E-5	0.0	0.0	5.492185233219488	0.0
9	8.739394744477576E-5	0.0	0.0	5.849102114583952	0.0
10	8.739394744477576E-5	0.0	0.0	7.501983842606997	0.0
11	8.739394744477576E-5	0.0	0.0	9.112916475856547	0.0
12	8.739394744477576E-5	0.0	0.0	11.314981769622563	0.0
13	8.739394744477576E-5	0.0	0.0	11.809981087949772	0.0
14	8.739394744477576E-5	0.0	0.0	11.997878074956041	0.0
15	8.739394744477576E-5	0.0	0.0	12.38013920107949	0.0
16	8.739394744477576E-5	0.0	0.0	13.055432232985272	0.0
17	8.739394744477576E-5	0.0	0.0	13.933654010857824	0.0
18	8.739394744477576E-5	0.0	0.0	14.833462093749235	0.0
19	8.739394744477576E-5	0.0	0.0	15.678386777645327	0.0
20	8.739394744477576E-5	0.0	0.0	16.367488053247385	0.0
21	8.739394744477576E-5	0.0	0.0	17.373741964126534	0.0
22	8.739394744477576E-5	0.0	0.0	18.492471885367106	0.0
23	8.739394744477576E-5	0.0	0.0	19.592761683696835	0.0
24	8.739394744477576E-5	0.0	0.0	20.43462757943236	0.0
25	1.7478789488955152E-4	0.0	0.0	21.172232495866265	0.0
26	1.7478789488955152E-4	0.0	0.0	21.81387885800581	0.0
27	1.7478789488955152E-4	0.0	0.0	22.463740251205163	0.0
28	1.7478789488955152E-4	0.0	0.0	23.111766371508175	0.0
29	1.7478789488955152E-4	0.0	0.0	23.811354920803605	0.0
30	1.7478789488955152E-4	0.0	0.0	24.56538989935713	0.0
31	1.7478789488955152E-4	0.0	0.0	25.260084387595654	0.0
32	1.7478789488955152E-4	0.0	0.0	25.92357923659639	0.0
33	1.7478789488955152E-4	0.0	0.0	26.60376632955908	0.0
34	1.7478789488955152E-4	0.0	0.0	27.255987359339443	0.0
35	1.7478789488955152E-4	0.0	0.0	27.955488514687428	0.0
36	1.7478789488955152E-4	0.0	0.0	28.616973302896934	0.0
37	1.7478789488955152E-4	0.0	0.0	29.284837849269913	0.0
38	1.7478789488955152E-4	0.0	0.0	29.934349666679484	0.0
39	1.7478789488955152E-4	0.0	0.0	30.596621000415997	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGCG	75	0.0	45.0	36
TATTGCG	25	3.8907954E-5	45.0	1
TCGTGCG	55	1.8189894E-12	45.0	1
TGCGATC	20	7.0332753E-4	45.0	14
GCGTATC	20	7.0332753E-4	45.0	22
TCGCGAG	20	7.0332753E-4	45.0	1
TAGGGCG	395	0.0	43.29114	5
AGGGCGA	565	0.0	42.21239	6
TTTAGGG	4425	0.0	41.898308	3
TAGGGTG	895	0.0	41.480446	5
CGTTTAT	575	0.0	41.086956	1
TTTTAGG	2725	0.0	40.37615	2
CGTTTTT	1505	0.0	40.365448	1
TACGGCT	1200	0.0	40.3125	7
TTAGGGA	2010	0.0	40.186565	4
TTAGGGT	1560	0.0	40.096153	4
TCGTTAG	135	0.0	40.000004	1
CGAGGGT	225	0.0	40.0	4
CGTTTGG	615	0.0	39.878048	2
TTGAGGG	1840	0.0	39.864132	3
>>END_MODULE