FastQCFastQC Report
Sat 18 Jun 2016
SRR3549693_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549693_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568105
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89800.5726657334808575No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33550.21395250955771458No Hit
CCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC23160.14769419139662204TruSeq Adapter, Index 21 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGCT21990.14023295633902066Illumina Paired End PCR Primer 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACG207.0340734E-445.00000439
TAGTTCG207.0340734E-445.0000041
CGTTTTT40950.042.527471
CGTTTAT16900.042.0710071
TTAGGGA36400.041.6002734
TTTAGGG53100.041.1016963
TAGGGCG5950.040.8403365
GGCGAAC501.0822987E-940.59
AGGGCGA9400.040.452136
TAGGGAA15250.039.983615
TAGGGAG17200.039.767445
CGTTAGG3850.039.740262
TAGGGAT26600.039.671055
CTAGGGA22000.039.4772724
TCTAGGG25650.039.1228073
TAGGGAC15800.039.018995
TCGAGGG7000.038.892863
ACACGAC3300.038.86363626
CTTAGGG27750.038.8378373
TGTAGGG22150.038.803613
Produced by FastQC (version 0.11.5)