##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549693_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1568105
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47400716151023	33.0	31.0	34.0	31.0	34.0
2	32.65820401057327	34.0	31.0	34.0	31.0	34.0
3	32.677080935269004	34.0	31.0	34.0	31.0	34.0
4	36.13285143533118	37.0	35.0	37.0	35.0	37.0
5	35.26028231527863	37.0	35.0	37.0	35.0	37.0
6	26.234053204345372	37.0	0.0	37.0	0.0	37.0
7	31.195898871567913	37.0	19.0	37.0	17.0	37.0
8	34.86325405505371	37.0	32.0	37.0	32.0	37.0
9	37.365991435522496	39.0	37.0	39.0	35.0	39.0
10	37.50281263053176	39.0	37.0	39.0	35.0	39.0
11	37.54324104572079	39.0	37.0	39.0	35.0	39.0
12	37.352450250461544	39.0	37.0	39.0	35.0	39.0
13	37.40281550023755	39.0	37.0	39.0	35.0	39.0
14	38.61280654037836	40.0	38.0	41.0	35.0	41.0
15	38.73773439916332	40.0	38.0	41.0	35.0	41.0
16	38.80208659496654	40.0	38.0	41.0	35.0	41.0
17	38.77308789908839	40.0	38.0	41.0	35.0	41.0
18	38.7235510377175	40.0	38.0	41.0	35.0	41.0
19	38.69599038329704	40.0	38.0	41.0	35.0	41.0
20	38.604749681940945	40.0	38.0	41.0	35.0	41.0
21	38.50326668175919	40.0	38.0	41.0	34.0	41.0
22	38.48547323042781	40.0	37.0	41.0	35.0	41.0
23	38.41986665433756	40.0	37.0	41.0	34.0	41.0
24	38.3823111335019	40.0	37.0	41.0	34.0	41.0
25	38.27933014689705	40.0	37.0	41.0	34.0	41.0
26	38.248601974995296	40.0	37.0	41.0	34.0	41.0
27	38.220292646219484	40.0	37.0	41.0	34.0	41.0
28	38.143327136894534	40.0	37.0	41.0	34.0	41.0
29	38.10976624652048	40.0	37.0	41.0	34.0	41.0
30	37.861969702283965	40.0	36.0	41.0	34.0	41.0
31	37.85318266315075	40.0	36.0	41.0	34.0	41.0
32	37.73810491006661	40.0	36.0	41.0	33.0	41.0
33	37.60152540805622	40.0	36.0	41.0	33.0	41.0
34	37.43864728446118	40.0	36.0	41.0	33.0	41.0
35	37.3626899984376	40.0	36.0	41.0	33.0	41.0
36	37.27717340356673	40.0	36.0	41.0	32.0	41.0
37	37.225184538025196	40.0	36.0	41.0	32.0	41.0
38	37.13160088131853	40.0	35.0	41.0	32.0	41.0
39	37.08096906776013	40.0	35.0	41.0	32.0	41.0
40	36.98461199983419	40.0	35.0	41.0	32.0	41.0
41	36.8414717126723	39.0	35.0	41.0	31.0	41.0
42	36.801919514318236	39.0	35.0	41.0	31.0	41.0
43	36.76823809630095	39.0	35.0	41.0	31.0	41.0
44	36.665026257807995	39.0	35.0	41.0	31.0	41.0
45	36.63498872843336	39.0	35.0	41.0	31.0	41.0
46	36.56307708986324	39.0	35.0	41.0	31.0	41.0
47	36.500888652226735	39.0	35.0	41.0	31.0	41.0
48	36.44747768803747	39.0	35.0	41.0	31.0	41.0
49	36.39205027724547	39.0	35.0	41.0	31.0	41.0
50	36.30700431412437	39.0	35.0	41.0	31.0	41.0
51	35.40901406474694	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	15.0
10	14.0
11	19.0
12	11.0
13	6.0
14	10.0
15	35.0
16	58.0
17	117.0
18	239.0
19	499.0
20	839.0
21	1519.0
22	2307.0
23	3670.0
24	5923.0
25	9780.0
26	13296.0
27	15324.0
28	16268.0
29	17284.0
30	20508.0
31	26057.0
32	35619.0
33	58184.0
34	88324.0
35	113633.0
36	136349.0
37	232681.0
38	350143.0
39	419063.0
40	302.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.042589622506146	3.911090137458907	54.18980234104222	18.856517898992735
2	21.672847162658112	3.998201651037399	52.99868312389795	21.330268062406535
3	20.120527643238177	4.071857433016284	53.70890342164587	22.09871150209967
4	18.432120298066774	4.575777769983516	52.390560581083534	24.601541350866174
5	18.198143619209173	7.7868510080638735	49.70285790811202	24.312147464614934
6	16.086614097907983	30.64660848603888	39.91218700278362	13.354590413269522
7	83.8910021969192	1.8561257058679108	10.239301577381617	4.013570519831261
8	85.88831742772327	2.197493152563126	8.402626099655317	3.511563320058287
9	80.3941700332567	4.938062183335937	10.158312102824747	4.509455680582614
10	38.73898750402556	32.078209048501215	16.281817862961983	12.90098558451124
11	26.868800239779862	24.01497348710705	30.099961418399918	19.01626485471317
12	26.733413897666292	21.160891649475	32.08905015926867	20.016644293590033
13	24.660721061408516	21.402010707191163	33.35242219111603	20.58484604028429
14	19.581214268177195	23.8991011443749	34.218945797634724	22.30073878981318
15	18.748043020078377	24.836028199642243	35.71482776982408	20.701101010455293
16	22.8082940874495	24.345117195595957	33.14943833480539	19.697150382149154
17	22.14819798419111	24.090542406280193	31.861960774310393	21.899298835218307
18	22.595361917728724	24.215597807544775	31.521103497533648	21.667936777192853
19	23.206417937574333	26.146973576386785	30.084018608447778	20.562589877591105
20	25.071344074535823	26.336565472337632	29.89551082357368	18.69657962955287
21	25.374576319825522	25.510536603097368	30.314232784156676	18.800654292920434
22	23.62380070212135	23.430318760542182	31.176675031327623	21.769205506008845
23	22.26636609155637	24.97913086177265	31.15588560715003	21.59861743952095
24	22.079516358917292	24.14863800574579	31.822550148108704	21.949295487228216
25	22.12925792596797	25.316034321681265	29.3480347298172	23.20667302253357
26	20.970343184927028	26.25563976902057	29.82644657086101	22.94757047519139
27	20.67469971717455	25.56270147726077	31.884344479483197	21.878254326081482
28	19.74612669432213	25.618692625812685	31.566444849037534	23.068735830827656
29	21.07996594615794	24.949541006501477	30.98886873009142	22.981624317249164
30	23.18709525191234	23.285494274936948	31.366777097196934	22.16063337595378
31	22.33747102394291	24.974475561266622	30.378705507603126	22.309347907187338
32	23.03984745919438	24.734568157106825	29.792073872604195	22.4335105110946
33	23.49415377159055	24.048325845526925	30.028027459895863	22.429492922986665
34	21.564754911182607	24.403722964980023	31.30281454366895	22.728707580168418
35	21.524005088944936	24.543509522640385	30.892893014179535	23.039592374235145
36	21.63802806572264	25.741834889883012	30.659107649041356	21.96102939535299
37	22.207313923493643	25.59331167236888	30.14262437783184	22.056750026305636
38	21.80000701483638	25.185877221231994	29.412379910784036	23.60173585314759
39	22.790756996502147	23.962744841703838	29.341338749637302	23.905159412156713
40	24.34186486236572	23.4282143096285	30.66491083186394	21.56500999614184
41	20.808938176971566	24.393965965289315	31.67415447307419	23.12294138466493
42	20.685221971742966	24.718242719715835	31.384569273103523	23.21196603543768
43	21.181617302412782	23.79942669655412	31.257983362083536	23.76097263894956
44	21.488293194652144	24.465198440155476	29.66312842571129	24.38337993948109
45	22.339065304938128	23.22650587811403	29.004180204769455	25.430248612178392
46	21.627314497434803	24.082507230064312	30.02464758418601	24.265530688314875
47	20.287353206577365	24.18103379556854	32.064370689462756	23.467242308391338
48	20.696254396229843	23.322290280306486	32.102697204587706	23.878758118875968
49	21.824367628443248	23.10438395388064	31.25511365629215	23.816134761383964
50	20.79414324933598	23.210307983202654	30.62639300301957	25.369155764441793
51	19.915311793534237	22.7322787695977	30.532649280500983	26.819760156367078
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	286.0
1	1020.5
2	1755.0
3	11391.5
4	21028.0
5	13552.5
6	6077.0
7	5882.0
8	5687.0
9	5667.0
10	5647.0
11	5468.5
12	5290.0
13	5122.5
14	4955.0
15	4825.0
16	4695.0
17	4564.5
18	4434.0
19	4625.5
20	4817.0
21	5471.5
22	6126.0
23	7131.5
24	8137.0
25	10086.5
26	14379.0
27	16722.0
28	20532.0
29	24342.0
30	27321.5
31	30301.0
32	34278.5
33	38256.0
34	43590.0
35	48924.0
36	52434.5
37	55945.0
38	61091.5
39	66238.0
40	71591.5
41	76945.0
42	82140.5
43	87336.0
44	93442.0
45	99548.0
46	111189.5
47	122831.0
48	132946.0
49	143061.0
50	139645.0
51	136229.0
52	122283.5
53	108338.0
54	97308.0
55	86278.0
56	81385.0
57	76492.0
58	73878.5
59	71265.0
60	65750.0
61	60235.0
62	52029.5
63	43824.0
64	36830.5
65	29837.0
66	25308.5
67	20780.0
68	16636.5
69	12493.0
70	10175.5
71	7858.0
72	6650.0
73	5442.0
74	4696.0
75	2927.5
76	1905.0
77	1346.0
78	787.0
79	657.0
80	527.0
81	381.5
82	236.0
83	173.5
84	111.0
85	86.0
86	61.0
87	41.5
88	22.0
89	17.5
90	13.0
91	7.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1568105.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.22160507021076
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.77938758791389	25.248490643555982
2	9.203350621933486	6.299068606129726
3	3.2261036246431676	3.3120733247434164
4	1.7940741590224545	2.455843893469477
5	1.2424069196101006	2.125857946969697
6	0.9802932525391602	2.012832512483251
7	0.8449739989152063	2.0241456539831013
8	0.7191146278949551	1.968740543682617
9	0.677514859779948	2.0867081364529736
>10	7.404232266632698	46.28888514039609
>50	0.08791455611330945	2.0167149841386163
>100	0.03722967130407125	2.1623326585039013
>500	0.001914667704909831	0.46659657784694863
>1k	0.001276445136606554	0.8779354239750901
>5k	2.127408561010923E-4	0.6537739536691525
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8980	0.5726657334808575	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3355	0.21395250955771458	No Hit
CCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	2316	0.14769419139662204	TruSeq Adapter, Index 21 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGCT	2199	0.14023295633902066	Illumina Paired End PCR Primer 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14801304759566483	0.0
2	0.0	0.0	0.0	0.5009868599360374	0.0
3	0.0	0.0	0.0	0.7671680148969616	0.0
4	0.0	0.0	0.0	1.0621099990115457	0.0
5	0.0	0.0	0.0	1.629036320909633	0.0
6	0.0	0.0	0.0	2.265473294199049	0.0
7	0.0	0.0	0.0	2.961600147949276	0.0
8	6.377123980855874E-5	0.0	0.0	3.921102222108851	0.0
9	6.377123980855874E-5	0.0	0.0	4.524952091856094	0.0
10	6.377123980855874E-5	0.0	0.0	5.366923771048495	0.0
11	6.377123980855874E-5	0.0	0.0	6.63788458043307	0.0
12	6.377123980855874E-5	0.0	0.0	7.692214488187972	0.0
13	6.377123980855874E-5	0.0	0.0	8.07101565265081	0.0
14	6.377123980855874E-5	0.0	0.0	8.242496516496026	0.0
15	1.2754247961711747E-4	0.0	0.0	8.441972954617198	0.0
16	1.2754247961711747E-4	0.0	0.0	8.84181862821686	0.0
17	1.2754247961711747E-4	0.0	0.0	9.408489865155714	0.0
18	1.2754247961711747E-4	0.0	0.0	9.992761964281728	0.0
19	1.2754247961711747E-4	0.0	0.0	10.433166146399635	0.0
20	1.2754247961711747E-4	0.0	0.0	10.924204692925537	0.0
21	1.913137194256762E-4	0.0	0.0	11.579071554519627	0.0
22	1.913137194256762E-4	0.0	0.0	12.327299511193447	0.0
23	1.913137194256762E-4	0.0	0.0	13.021194371550374	0.0
24	1.913137194256762E-4	0.0	0.0	13.56120923024925	0.0
25	1.913137194256762E-4	0.0	0.0	14.031522123837371	0.0
26	1.913137194256762E-4	0.0	0.0	14.46918414264351	0.0
27	1.913137194256762E-4	0.0	0.0	14.912075403113949	0.0
28	1.913137194256762E-4	0.0	0.0	15.366764342948974	0.0
29	2.5508495923423494E-4	0.0	0.0	15.860034882868176	0.0
30	2.5508495923423494E-4	0.0	0.0	16.422050819301003	0.0
31	2.5508495923423494E-4	0.0	0.0	16.911495084831692	0.0
32	2.5508495923423494E-4	0.0	0.0	17.383848658093687	0.0
33	2.5508495923423494E-4	0.0	0.0	17.85530943399836	0.0
34	2.5508495923423494E-4	0.0	0.0	18.34252170613575	0.0
35	2.5508495923423494E-4	0.0	0.0	18.85167128476728	0.0
36	2.5508495923423494E-4	0.0	0.0	19.363116628031925	0.0
37	2.5508495923423494E-4	0.0	0.0	19.87309523278097	0.0
38	3.188561990427937E-4	0.0	0.0	20.409347588331137	0.0
39	3.188561990427937E-4	0.0	0.0	21.11905771616059	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAACG	20	7.0340734E-4	45.000004	39
TAGTTCG	20	7.0340734E-4	45.000004	1
CGTTTTT	4095	0.0	42.52747	1
CGTTTAT	1690	0.0	42.071007	1
TTAGGGA	3640	0.0	41.600273	4
TTTAGGG	5310	0.0	41.101696	3
TAGGGCG	595	0.0	40.840336	5
GGCGAAC	50	1.0822987E-9	40.5	9
AGGGCGA	940	0.0	40.45213	6
TAGGGAA	1525	0.0	39.98361	5
TAGGGAG	1720	0.0	39.76744	5
CGTTAGG	385	0.0	39.74026	2
TAGGGAT	2660	0.0	39.67105	5
CTAGGGA	2200	0.0	39.477272	4
TCTAGGG	2565	0.0	39.122807	3
TAGGGAC	1580	0.0	39.01899	5
TCGAGGG	700	0.0	38.89286	3
ACACGAC	330	0.0	38.863636	26
CTTAGGG	2775	0.0	38.837837	3
TGTAGGG	2215	0.0	38.80361	3
>>END_MODULE