FastQCFastQC Report
Sat 18 Jun 2016
SRR3549692_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549692_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431520
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGC193154.476038190582128No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCG165923.845012977382277No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTC128892.9868835743418614No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTTGTCTT48281.1188357434186134No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCT43901.0173340748980348No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTC40730.9438728216536894No Hit
GCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC36930.8558120133481647No Hit
GAATCAGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTC33660.7800333704115684No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTA33160.7684464219503152No Hit
GAACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCT27860.6456247682610308No Hit
CCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC20350.47158880237300704No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20040.46440489432703No Hit
CTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGCT17660.40925101965146465No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGT12260.28411197626992957No Hit
GCTGTATCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC10770.24958286985539488No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATG10120.2345198368557657No Hit
TCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC10050.2328976640711902No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTAT8460.1960511679644049No Hit
GAACTATCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCT8060.1867816091954023No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7130.16522988505747127No Hit
CCTGTATCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC6260.14506859473489062No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTT6020.13950685947348906No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGC5890.1364942528735632No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGGTTGTCT5880.13626251390433816No Hit
CGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTG5190.12027252502780869No Hit
CTGTCACTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGCT4960.11494252873563218No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCG4480.10381905821282907No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCAT850.045.00000417
ACTCCGT351.2095734E-745.00000440
TCGTTAG406.7975634E-945.01
GGGTACG253.886743E-545.07
TGGAACG207.028385E-445.021
ACCGAAT207.028385E-445.017
GGTTTAG253.886743E-545.01
GCGTTCG453.8380676E-1045.01
ATCCGCA253.886743E-545.011
CAGGGCG900.045.05
ACGCTAG207.028385E-445.018
GTATACT406.7975634E-945.011
TAGTCGT207.028385E-445.023
TGGTCGA207.028385E-445.013
CGCTTTA207.028385E-445.013
CCTGTTG302.16234E-644.9999961
ATGATCC1750.043.7142873
ACGGCTG22500.043.28
TGATCCG1250.043.1999974
TACGGCT22600.043.108417
Produced by FastQC (version 0.11.5)