##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549692_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	431520
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03175287356322	33.0	31.0	34.0	30.0	34.0
2	32.15877131998517	33.0	31.0	34.0	30.0	34.0
3	32.25678068223952	34.0	31.0	34.0	30.0	34.0
4	35.8722469410456	37.0	35.0	37.0	35.0	37.0
5	35.09226223581757	37.0	35.0	37.0	35.0	37.0
6	26.963888116425657	35.0	17.0	37.0	0.0	37.0
7	31.44006071560994	35.0	28.0	37.0	17.0	37.0
8	34.12441601779755	35.0	32.0	37.0	30.0	37.0
9	36.83954857248795	39.0	37.0	39.0	34.0	39.0
10	37.10665090841676	39.0	37.0	39.0	34.0	39.0
11	37.173062662217276	39.0	37.0	39.0	34.0	39.0
12	36.854039210233594	39.0	35.0	39.0	33.0	39.0
13	36.67888394512421	39.0	35.0	39.0	32.0	39.0
14	37.852463385242864	40.0	36.0	41.0	33.0	41.0
15	37.78531470152021	40.0	36.0	41.0	33.0	41.0
16	38.12362810530219	40.0	37.0	41.0	34.0	41.0
17	37.86254866518354	40.0	36.0	41.0	33.0	41.0
18	37.87952817945866	40.0	36.0	41.0	33.0	41.0
19	37.90025723025584	40.0	36.0	41.0	33.0	41.0
20	37.57549592139414	40.0	35.0	41.0	32.0	41.0
21	37.72725018539118	39.0	36.0	41.0	33.0	41.0
22	37.71881488691138	39.0	36.0	41.0	33.0	41.0
23	37.597038375973305	39.0	35.0	41.0	33.0	41.0
24	37.51617538005191	39.0	35.0	41.0	33.0	41.0
25	37.469971264367814	39.0	35.0	41.0	33.0	41.0
26	37.46033787541713	39.0	35.0	41.0	33.0	41.0
27	37.32687708565072	39.0	35.0	41.0	33.0	41.0
28	37.20138116425658	39.0	35.0	41.0	32.0	41.0
29	37.2319660734149	39.0	35.0	41.0	32.0	41.0
30	36.96481275491286	39.0	35.0	41.0	31.0	41.0
31	36.87037217278458	39.0	35.0	41.0	31.0	41.0
32	36.746938728216534	39.0	35.0	41.0	31.0	41.0
33	36.644169447534296	39.0	35.0	41.0	31.0	41.0
34	36.52416805710048	39.0	35.0	41.0	30.0	41.0
35	36.56554736744531	39.0	35.0	41.0	31.0	41.0
36	36.27972052280312	39.0	35.0	41.0	30.0	41.0
37	36.28657536151279	39.0	35.0	40.0	30.0	41.0
38	35.85663005190953	38.0	35.0	40.0	29.0	41.0
39	36.07253661475714	39.0	35.0	40.0	30.0	41.0
40	36.05160826844642	39.0	35.0	40.0	30.0	41.0
41	35.74750648869114	38.0	35.0	40.0	27.0	41.0
42	35.951575824990734	38.0	35.0	40.0	29.0	41.0
43	35.89771968854283	38.0	35.0	40.0	29.0	41.0
44	35.877231646273636	38.0	35.0	40.0	29.0	41.0
45	35.6393539117538	38.0	35.0	40.0	28.0	41.0
46	35.635755005561734	38.0	35.0	40.0	28.0	41.0
47	35.354546718576195	38.0	34.0	40.0	27.0	41.0
48	35.36620550611791	38.0	34.0	40.0	28.0	41.0
49	35.44494345569151	38.0	35.0	40.0	28.0	41.0
50	35.30591397849462	38.0	34.0	40.0	27.0	41.0
51	34.34491796440489	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	4.0
12	1.0
13	4.0
14	3.0
15	8.0
16	23.0
17	57.0
18	174.0
19	326.0
20	595.0
21	1110.0
22	1706.0
23	2232.0
24	2904.0
25	3471.0
26	4254.0
27	4607.0
28	5379.0
29	6313.0
30	7999.0
31	10899.0
32	15011.0
33	22062.0
34	31875.0
35	41590.0
36	41154.0
37	61323.0
38	83859.0
39	82528.0
40	45.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.66671301446051	3.1233778272154242	39.253568780126066	16.956340378198
2	16.12370226177234	25.898683722654802	39.99374304783092	17.983870967741936
3	15.439840563589174	25.86531331108639	42.34774749721913	16.347098628105304
4	16.130886169818318	3.485585836114201	61.12184835001854	19.261679644048943
5	30.47483314794216	6.26830737856878	40.75083426028921	22.50602521319985
6	13.224647756766778	39.94924916573971	35.2736837226548	11.55241935483871
7	65.82545420837968	1.796672228401928	27.309510567296996	5.068362995921394
8	62.50625695216908	16.719271412680754	13.267519466073416	7.506952169076751
9	59.55112161661105	3.5622914349276975	12.932656655543195	23.95393029291806
10	43.10877827215425	19.725157582499072	20.97585279940675	16.190211345939932
11	34.132832777159805	19.27627919911012	27.02423989618094	19.56664812754913
12	26.59992584352985	16.272710418984058	31.286846496106786	25.840517241379313
13	22.73799592139414	21.882647385984427	39.23688357434186	16.14247311827957
14	19.10734149054505	31.36934556915091	26.651140155728587	22.872172784575454
15	12.287958843159066	20.547599184278827	45.785826844642195	21.37861512791991
16	13.953698553948831	22.452030033370413	30.639831294030408	32.95444011865035
17	15.095939933259178	23.695309603262885	35.22131071560994	25.987439747868002
18	16.06205969595847	22.62629773822766	33.59403967371153	27.717602892102335
19	17.286336670374492	25.29152762328513	29.51589729328884	27.906238413051536
20	21.78207267334075	24.033416759362254	37.243233222098624	16.94127734519837
21	20.505886169818314	32.20082499073044	28.33101594364108	18.96227289581016
22	17.07614942528736	21.181637004078606	32.9539766407119	28.788236929922135
23	18.294401186503524	30.460465331850205	29.297599184278827	21.947534297367447
24	21.962133852428625	22.045791620318873	27.776464590285503	28.215609936967002
25	15.933908045977011	34.42181127178346	25.64794215795328	23.996338524286244
26	19.029940674823877	22.902298850574713	31.114432703003338	26.953327771598072
27	23.832267334074896	27.602660363366706	26.185344827586203	22.379727474972192
28	15.321885428253616	23.909436410826846	37.90299406748239	22.865684093437153
29	26.405265109380792	17.480997404523542	31.709770114942533	24.403967371153133
30	24.145346681497962	26.3461716722284	31.31210604375232	18.19637560252132
31	24.111512791991103	20.713987764182427	30.181451612903228	24.99304783092325
32	27.069428995179827	26.695402298850574	27.332452725250278	18.902715980719318
33	24.95388394512421	20.494067482387837	26.63700407860586	27.915044493882093
34	26.32044864664442	20.204162031887282	31.755422691879865	21.71996662958843
35	24.247311827956988	18.978958101594365	27.455737856878017	29.317992213570633
36	26.63028364849833	19.611142009640343	33.87444382647386	19.88413051538747
37	23.125695216907673	20.111234705228032	38.077493511308866	18.685576566555433
38	23.451288468668892	20.537170930663702	31.099369670003707	24.9121709306637
39	22.4121709306637	20.130700778642936	39.41138301816834	18.045745272525025
40	25.21644419725621	18.655682239525397	30.36916017797553	25.758713385242864
41	18.730533926585093	21.829579162031887	35.13301816833519	24.30686874304783
42	19.841490545050057	18.227196885428253	33.95277159807193	27.97854097144976
43	25.25815721171672	18.959955506117907	31.414534668149795	24.367352614015573
44	23.546996662958843	19.118696700037077	32.696978123841305	24.637328513162775
45	20.22455506117909	18.63250834260289	30.061410826844643	31.081525769373382
46	29.336531331108638	22.70810159436411	28.77641824249166	19.178948832035594
47	18.252919911012235	19.17431405265109	40.97307193177605	21.599694104560623
48	21.379773822766033	21.76700964034112	30.24425287356322	26.608963663329625
49	19.46700037078235	15.988598442714128	40.397664071190206	24.14673711531331
50	23.339126807563957	16.90211345939933	33.38431590656285	26.374443826473858
51	20.425704486466444	18.348859844271413	28.052697441601783	33.17273822766037
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	82.0
1	266.0
2	450.0
3	2332.0
4	4214.0
5	2762.0
6	1310.0
7	1292.5
8	1275.0
9	1334.5
10	1394.0
11	1433.0
12	1472.0
13	1512.5
14	1553.0
15	1431.0
16	1309.0
17	1222.0
18	1135.0
19	1152.0
20	1169.0
21	1207.0
22	1245.0
23	1300.5
24	1356.0
25	1658.0
26	2163.0
27	2366.0
28	2981.5
29	3597.0
30	4285.5
31	4974.0
32	5654.0
33	6334.0
34	7060.0
35	7786.0
36	8544.5
37	9303.0
38	10496.0
39	11689.0
40	14116.0
41	16543.0
42	18756.5
43	20970.0
44	24935.0
45	28900.0
46	64836.0
47	100772.0
48	75937.5
49	51103.0
50	46364.5
51	41626.0
52	33440.5
53	25255.0
54	22011.0
55	18767.0
56	17083.0
57	15399.0
58	14174.5
59	12950.0
60	11925.5
61	10901.0
62	9424.0
63	7947.0
64	6590.0
65	5233.0
66	4151.5
67	3070.0
68	2732.0
69	2394.0
70	1961.0
71	1528.0
72	1284.0
73	1040.0
74	802.0
75	440.5
76	317.0
77	235.5
78	154.0
79	102.5
80	51.0
81	43.0
82	35.0
83	26.5
84	18.0
85	10.5
86	3.0
87	3.0
88	3.0
89	2.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	431520.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.23379422288806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.3097713804122	23.95285879426063
2	10.175713500704875	6.560037101055697
3	3.796144382082925	3.670924105573006
4	2.0809928598704874	2.6831318249745846
5	1.4570484883851615	2.3483100573688698
6	1.0927495050225209	2.113407760923523
7	0.8540184948551502	1.9269779497991044
8	0.6719398363735741	1.7327336332661487
9	0.5464019094570842	1.585134604118949
>10	4.87250492336598	28.248086537998546
>50	0.08649376937577387	1.9216444141255202
>100	0.03820141480763345	2.1927795889876265
>500	0.005766251291718257	1.2288208567657932
>1k	0.010090939760506951	8.498124499512654
>5k	0.0	0.0
>10k+	0.0021623442343943465	11.33702827126936
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGC	19315	4.476038190582128	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCG	16592	3.845012977382277	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTC	12889	2.9868835743418614	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTTGTCTT	4828	1.1188357434186134	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCT	4390	1.0173340748980348	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTC	4073	0.9438728216536894	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	3693	0.8558120133481647	No Hit
GAATCAGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTC	3366	0.7800333704115684	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTA	3316	0.7684464219503152	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCT	2786	0.6456247682610308	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	2035	0.47158880237300704	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2004	0.46440489432703	No Hit
CTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGCT	1766	0.40925101965146465	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGT	1226	0.28411197626992957	No Hit
GCTGTATCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	1077	0.24958286985539488	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATG	1012	0.2345198368557657	No Hit
TCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	1005	0.2328976640711902	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTAT	846	0.1960511679644049	No Hit
GAACTATCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCT	806	0.1867816091954023	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	713	0.16522988505747127	No Hit
CCTGTATCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	626	0.14506859473489062	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTT	602	0.13950685947348906	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGC	589	0.1364942528735632	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGGTTGTCT	588	0.13626251390433816	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTG	519	0.12027252502780869	No Hit
CTGTCACTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGCT	496	0.11494252873563218	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCG	448	0.10381905821282907	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.43126622172784573	0.0
2	0.0	0.0	0.0	2.113691138301817	0.0
3	0.0	0.0	0.0	2.6666203188728215	0.0
4	0.0	0.0	0.0	3.8331942899517983	0.0
5	0.0	0.0	0.0	7.814238042269188	0.0
6	0.0	0.0	0.0	8.410734149054505	0.0
7	0.0	0.0	0.0	10.097330367074527	0.0
8	0.0	0.0	0.0	12.016129032258064	0.0
9	0.0	0.0	0.0	12.44600482017056	0.0
10	0.0	0.0	0.0	17.41981831664813	0.0
11	0.0	0.0	0.0	19.05149239896181	0.0
12	0.0	0.0	0.0	25.433120133481648	0.0
13	0.0	0.0	0.0	26.235400444938822	0.0
14	0.0	0.0	0.0	26.634918427882834	0.0
15	0.0	0.0	0.0	27.782026325546905	0.0
16	0.0	0.0	0.0	28.662402669632925	0.0
17	0.0	0.0	0.0	29.53652206154987	0.0
18	0.0	0.0	0.0	30.350157582499072	0.0
19	0.0	0.0	0.0	32.37903225806452	0.0
20	0.0	0.0	0.0	33.09464219503152	0.0
21	0.0	0.0	0.0	33.95045420837968	0.0
22	0.0	0.0	0.0	34.93997960697071	0.0
23	0.0	0.0	0.0	35.74921208750464	0.0
24	0.0	0.0	0.0	36.39460511679644	0.0
25	0.0	0.0	0.0	36.91370040786059	0.0
26	0.0	0.0	0.0	37.43325917686318	0.0
27	0.0	0.0	0.0	38.00380051909529	0.0
28	0.0	0.0	0.0	38.46449758991472	0.0
29	2.317389692250649E-4	0.0	0.0	38.971542454579165	0.0
30	2.317389692250649E-4	0.0	0.0	39.53513162773452	0.0
31	2.317389692250649E-4	0.0	0.0	40.06905821282907	0.0
32	2.317389692250649E-4	0.0	0.0	40.55640526510938	0.0
33	2.317389692250649E-4	0.0	0.0	41.03193362995921	0.0
34	2.317389692250649E-4	0.0	0.0	41.514414163885796	0.0
35	2.317389692250649E-4	0.0	0.0	42.05923248053393	0.0
36	2.317389692250649E-4	0.0	0.0	42.5757786429366	0.0
37	2.317389692250649E-4	0.0	0.0	43.05177048572488	0.0
38	2.317389692250649E-4	0.0	0.0	43.51802929180571	0.0
39	2.317389692250649E-4	0.0	0.0	43.981507230255836	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCAT	85	0.0	45.000004	17
ACTCCGT	35	1.2095734E-7	45.000004	40
TCGTTAG	40	6.7975634E-9	45.0	1
GGGTACG	25	3.886743E-5	45.0	7
TGGAACG	20	7.028385E-4	45.0	21
ACCGAAT	20	7.028385E-4	45.0	17
GGTTTAG	25	3.886743E-5	45.0	1
GCGTTCG	45	3.8380676E-10	45.0	1
ATCCGCA	25	3.886743E-5	45.0	11
CAGGGCG	90	0.0	45.0	5
ACGCTAG	20	7.028385E-4	45.0	18
GTATACT	40	6.7975634E-9	45.0	11
TAGTCGT	20	7.028385E-4	45.0	23
TGGTCGA	20	7.028385E-4	45.0	13
CGCTTTA	20	7.028385E-4	45.0	13
CCTGTTG	30	2.16234E-6	44.999996	1
ATGATCC	175	0.0	43.714287	3
ACGGCTG	2250	0.0	43.2	8
TGATCCG	125	0.0	43.199997	4
TACGGCT	2260	0.0	43.10841	7
>>END_MODULE