SRR3549691_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549691_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1299411
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7188	0.553173707164246	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2749	0.21155739023295939	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	2573	0.19801279194958332	TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGCT	2369	0.18231337121203375	TruSeq Adapter, Index 23 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	1782	0.13713905761918285	TruSeq Adapter, Index 20 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	1500	0.11543691718786434	TruSeq Adapter, Index 20 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTG	1403	0.10797199654304912	TruSeq Adapter, Index 23 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGCG	20	7.0336263E-4	45.000004	1
CGTTTAT	1475	0.0	43.169495	1
CGTTTTT	3155	0.0	42.86054	1
CGTTAGG	375	0.0	42.600002	2
TAGGGAT	2240	0.0	42.589287	5
TTAGGGA	3700	0.0	42.26351	4
TCGTTAG	220	0.0	40.909092	1
TGTAGGG	1700	0.0	40.897057	3
TAGGGAA	1435	0.0	40.766552	5
TTTAGGG	5245	0.0	40.753098	3
CCGTAGG	100	0.0	40.499996	2
TTTTAGG	2905	0.0	40.430294	2
AGGGATC	935	0.0	40.427807	6
CTAGGGA	1765	0.0	40.410767	4
TAGGGAC	1465	0.0	40.392494	5
TAGGGCG	535	0.0	40.373833	5
AGGGATA	865	0.0	40.057804	6
CGTTCGG	90	0.0	40.0	2
TATTGCG	45	1.9286745E-8	40.0	1
TGCGTAG	90	0.0	40.0	1