FastQCFastQC Report
Sat 18 Jun 2016
SRR3549688_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549688_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778891
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGCT64460.8275869152423124TruSeq Adapter, Index 20 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC57460.7377155468480185TruSeq Adapter, Index 23 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC44280.5685005989284765TruSeq Adapter, Index 23 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC32230.4137934576211562TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCACTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGCT17170.22044162790428953TruSeq Adapter, Index 20 (96% over 25bp)
TCTGTATCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC16640.21363708144015017TruSeq Adapter, Index 20 (95% over 24bp)
CCTGTATCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC16280.20901512535130076TruSeq Adapter, Index 20 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG16010.20554865828466368TruSeq Adapter, Index 20 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15990.20529188294639428No Hit
TCCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG14050.1803846751342614TruSeq Adapter, Index 20 (95% over 23bp)
GCTGTATCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC13060.16767429588992555TruSeq Adapter, Index 20 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCC11360.14584839213702558No Hit
TTCCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCT9940.1276173431198974No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTC8770.11259598583113684TruSeq Adapter, Index 20 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG8510.10925790643363448TruSeq Adapter, Index 23 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG8230.10566305169786272TruSeq Adapter, Index 20 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG302.1645974E-645.00000429
CCGTACG207.031884E-445.01
TCGTCAG207.031884E-445.01
TCCGTTG406.8102963E-945.01
CTAGGGA7800.042.980774
TAGGGCG3100.042.822585
TCTTGCG1750.042.4285741
TCGTTAG750.042.01
TTAGGGA13650.041.8681344
CTCGTAG700.041.7857131
CTTAGGG11400.041.6447373
GTTTTAG3950.041.582281
TAGGGAC5150.041.5048525
CGTTTAT3600.041.251
TAGGGAT7050.041.170215
TAGGGAG9850.041.116755
TCTTAGG6700.040.970152
TTTAGGG25950.040.924863
CGTTTTT9950.040.7035181
TTGAGGG10050.040.522393
Produced by FastQC (version 0.11.5)