##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549688_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	778891
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.246838132678384	33.0	31.0	34.0	30.0	34.0
2	32.41952981867809	34.0	31.0	34.0	30.0	34.0
3	32.47611154834245	34.0	31.0	34.0	30.0	34.0
4	35.973259416272626	37.0	35.0	37.0	35.0	37.0
5	35.08504655978821	37.0	35.0	37.0	35.0	37.0
6	25.968280542463578	36.0	0.0	37.0	0.0	37.0
7	30.993905437346175	37.0	19.0	37.0	17.0	37.0
8	34.613350263387304	37.0	32.0	37.0	32.0	37.0
9	37.10981510891768	39.0	37.0	39.0	34.0	39.0
10	37.27190454120024	39.0	37.0	39.0	34.0	39.0
11	37.36261042944391	39.0	37.0	39.0	35.0	39.0
12	37.151996877611886	39.0	37.0	39.0	34.0	39.0
13	37.16008658464406	39.0	37.0	39.0	34.0	39.0
14	38.31727160796569	40.0	38.0	41.0	34.0	41.0
15	38.427480867027604	40.0	38.0	41.0	34.0	41.0
16	38.48409212585587	40.0	38.0	41.0	34.0	41.0
17	38.45686238510908	40.0	38.0	41.0	34.0	41.0
18	38.45613442702509	40.0	38.0	41.0	34.0	41.0
19	38.474257630400146	40.0	37.0	41.0	34.0	41.0
20	38.420409274211664	40.0	37.0	41.0	34.0	41.0
21	38.311495446731314	40.0	37.0	41.0	34.0	41.0
22	38.28941790314691	40.0	37.0	41.0	34.0	41.0
23	38.24919147865362	40.0	37.0	41.0	34.0	41.0
24	38.19674896744217	40.0	37.0	41.0	34.0	41.0
25	38.09474368043796	40.0	37.0	41.0	34.0	41.0
26	38.03019164427372	40.0	37.0	41.0	34.0	41.0
27	37.967284254151096	40.0	36.0	41.0	34.0	41.0
28	37.90906558170527	40.0	36.0	41.0	34.0	41.0
29	37.87695967728475	40.0	36.0	41.0	34.0	41.0
30	37.63122439468424	40.0	36.0	41.0	33.0	41.0
31	37.67858147032126	40.0	36.0	41.0	33.0	41.0
32	37.643086131435595	40.0	36.0	41.0	33.0	41.0
33	37.57783951798133	40.0	36.0	41.0	33.0	41.0
34	37.544574272908534	40.0	36.0	41.0	33.0	41.0
35	37.52631112697412	40.0	36.0	41.0	33.0	41.0
36	37.44525613981931	40.0	36.0	41.0	33.0	41.0
37	37.36770998766195	40.0	35.0	41.0	33.0	41.0
38	37.28716983506036	40.0	35.0	41.0	33.0	41.0
39	37.26386747311241	39.0	35.0	41.0	33.0	41.0
40	37.182506923305056	39.0	35.0	41.0	33.0	41.0
41	37.1079714619889	39.0	35.0	41.0	32.0	41.0
42	37.08972885808156	39.0	35.0	41.0	32.0	41.0
43	36.98532914104798	39.0	35.0	41.0	32.0	41.0
44	36.82647892965768	39.0	35.0	41.0	31.0	41.0
45	36.79461054242506	39.0	35.0	41.0	31.0	41.0
46	36.70425001701137	39.0	35.0	40.0	31.0	41.0
47	36.645814369404704	39.0	35.0	40.0	31.0	41.0
48	36.643965586969166	39.0	35.0	40.0	31.0	41.0
49	36.602210065336486	39.0	35.0	40.0	31.0	41.0
50	36.51114725937262	39.0	35.0	40.0	31.0	41.0
51	35.56139434144187	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	5.0
10	12.0
11	8.0
12	5.0
13	8.0
14	9.0
15	20.0
16	26.0
17	63.0
18	110.0
19	227.0
20	431.0
21	652.0
22	1053.0
23	1625.0
24	2335.0
25	3305.0
26	4542.0
27	5668.0
28	6716.0
29	8226.0
30	10719.0
31	14289.0
32	20081.0
33	32664.0
34	48481.0
35	65324.0
36	76813.0
37	125902.0
38	173540.0
39	175933.0
40	96.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.63541111657472	4.307406299469374	52.504394067976136	18.55278851597977
2	20.71419492586254	4.689488002814258	51.60953201410724	22.986785057215965
3	21.289243295916886	4.790015547746732	51.10869171681275	22.81204943952363
4	20.509930144269223	4.727490752878131	50.01624104014554	24.746338062707103
5	18.22783932539983	8.061718520306435	48.020968274123085	25.689473880170656
6	16.062966448450425	31.273695549184676	38.699124781259506	13.964213221105393
7	78.94159773318732	2.312390308785183	12.24908234913486	6.496929608892643
8	78.29644969578541	3.113529364185746	10.81858693963597	7.7714340003928655
9	72.61105854349324	5.408587337637743	14.18349935998747	7.796854758881539
10	37.14755979976659	26.98760160279166	20.37833278340615	15.486505814035597
11	28.269295703763426	25.92699106806986	26.735833383618502	19.067879844548212
12	28.685014976421602	20.666306325275297	31.315421541653453	19.333257156649648
13	24.464655516625562	22.79728485757314	30.755266141218733	21.982793484582565
14	17.83458789484023	27.486644472718265	30.10254323133789	24.57622440110362
15	16.707472547506647	26.71798749760878	34.90565432133636	21.668885633548214
16	18.532118101249083	25.06127301509454	33.41096507727011	22.99564380638626
17	17.487299249830848	25.285566273072867	30.224896680023267	27.00223779707302
18	19.136952410542683	25.434367581599993	30.98649233333034	24.442187674526988
19	21.608415041385765	27.617728282904796	28.245158821966104	22.528697853743335
20	26.629656781244105	25.457990912720778	28.472019833327128	19.44033247270799
21	24.74210126962566	26.65045558364392	28.039738551350574	20.56770459537984
22	20.429302688052626	26.81427824945981	27.155275898681587	25.601143163805972
23	22.944288738732375	25.957290557985647	26.867045581474176	24.2313751218078
24	24.08681060636212	22.78855449607198	27.724418435955737	25.40021646161016
25	20.589658886801875	24.725282484969014	25.80502278239189	28.88003584583722
26	20.064938483048333	27.658812337027904	26.151412713717328	26.124836466206443
27	21.504806192394057	28.31705591668154	26.616304463654096	23.56183342727031
28	20.094082483941914	27.23423431519943	27.10481954471165	25.56686365614701
29	20.36960242190499	25.024168978714606	26.479443208356496	28.126785391023905
30	21.956474012409952	24.41175979694206	26.49420779030699	27.137558400341
31	21.291811049299582	26.818515042541254	26.42718942701867	25.462484481140496
32	21.661824311745804	26.032654119767724	26.89593280702948	25.409588761456998
33	21.648471994155795	25.372227949738797	28.31577203999019	24.663528016115222
34	20.125409075210783	23.38119197679778	30.76990233550009	25.72349661249135
35	19.533285145161518	22.748112380294547	28.63443023478253	29.0841722397614
36	20.016921494791955	26.86178168703965	28.353261239377524	24.76803557879087
37	21.96867084097775	26.826603465696742	29.40976336868702	21.794962324638494
38	20.416463921139158	25.536307390892947	28.12100794591284	25.92622074205505
39	21.72280845458479	22.592634912972418	29.68977687506981	25.99477975737298
40	22.198613156398007	21.655148152950797	31.664764389368987	24.48147430128221
41	17.526200713578667	24.791787297580793	31.392967693810814	26.28904429502973
42	21.200270641206536	23.749664587214387	29.231689671597184	25.8183750999819
43	22.573761925609617	23.897952345064972	28.63057860470849	24.897707124616925
44	21.384892109422243	23.03993755223773	27.04357862653439	28.531591711805632
45	21.41185351994053	21.23609080089512	26.36389430613526	30.988161373029087
46	20.431998829104458	23.814371972458275	29.749091978210046	26.00453722022722
47	18.546369132523036	23.613958820939	32.33571834826696	25.503953698271005
48	18.53686844500707	21.343166116953462	32.30798661173387	27.811978826305605
49	20.06750623643103	21.235834025556848	32.84169415232683	25.854965585685292
50	19.577193728005586	20.74012923502775	30.141573082754842	29.541103954211824
51	17.42451767962398	21.09512114018521	29.238365830392187	32.24199534979862
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	239.0
1	559.5
2	880.0
3	2390.0
4	3900.0
5	2690.0
6	1480.0
7	1590.5
8	1701.0
9	1882.0
10	2063.0
11	2260.5
12	2458.0
13	2370.0
14	2282.0
15	2277.0
16	2272.0
17	2328.0
18	2384.0
19	2396.0
20	2408.0
21	2600.5
22	2793.0
23	3101.0
24	3409.0
25	4007.5
26	5394.5
27	6183.0
28	7259.5
29	8336.0
30	9613.5
31	10891.0
32	12693.0
33	14495.0
34	15982.0
35	17469.0
36	18429.5
37	19390.0
38	21802.0
39	24214.0
40	28005.5
41	31797.0
42	36110.0
43	40423.0
44	47079.0
45	53735.0
46	63186.0
47	72637.0
48	85773.0
49	98909.0
50	92095.0
51	85281.0
52	70285.5
53	55290.0
54	48135.5
55	40981.0
56	38576.5
57	36172.0
58	36597.5
59	37023.0
60	34381.0
61	31739.0
62	27062.0
63	22385.0
64	18106.0
65	13827.0
66	11327.0
67	8827.0
68	7364.5
69	5902.0
70	4740.0
71	3578.0
72	3267.0
73	2956.0
74	2263.5
75	1250.0
76	929.0
77	761.0
78	593.0
79	430.5
80	268.0
81	188.0
82	108.0
83	70.0
84	32.0
85	37.0
86	42.0
87	35.0
88	28.0
89	15.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	778891.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.32182313901785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.18673772815394	26.219539207446747
2	10.910679756707783	7.925915608992047
3	4.061488253025174	4.4256197402273685
4	2.1313706621501614	3.096610729372381
5	1.4586764725718278	2.6490894426900176
6	1.1567456953909938	2.5209067538887133
7	0.9685061459096511	2.462453625854751
8	0.8137710596683528	2.3646118803940044
9	0.6710316452448755	2.193578346534168
>10	5.451386425212656	33.82751462948864
>50	0.12354259518365543	3.075896498240517
>100	0.058225511196680915	4.016329819404672
>500	0.0033591641075008077	0.8974584285579854
>1k	0.0037324045638897864	2.671633421690667
>5k	7.464809127779574E-4	1.6528418672173653
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGCT	6446	0.8275869152423124	TruSeq Adapter, Index 20 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	5746	0.7377155468480185	TruSeq Adapter, Index 23 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	4428	0.5685005989284765	TruSeq Adapter, Index 23 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	3223	0.4137934576211562	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCACTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGCT	1717	0.22044162790428953	TruSeq Adapter, Index 20 (96% over 25bp)
TCTGTATCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	1664	0.21363708144015017	TruSeq Adapter, Index 20 (95% over 24bp)
CCTGTATCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	1628	0.20901512535130076	TruSeq Adapter, Index 20 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG	1601	0.20554865828466368	TruSeq Adapter, Index 20 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1599	0.20529188294639428	No Hit
TCCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG	1405	0.1803846751342614	TruSeq Adapter, Index 20 (95% over 23bp)
GCTGTATCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	1306	0.16767429588992555	TruSeq Adapter, Index 20 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCC	1136	0.14584839213702558	No Hit
TTCCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCT	994	0.1276173431198974	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTC	877	0.11259598583113684	TruSeq Adapter, Index 20 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG	851	0.10925790643363448	TruSeq Adapter, Index 23 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTG	823	0.10566305169786272	TruSeq Adapter, Index 20 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2838766913470561E-4	0.0	0.0	0.8955039922145718	0.0
2	1.2838766913470561E-4	0.0	0.0	2.8075815486377427	0.0
3	1.2838766913470561E-4	0.0	0.0	3.8194047690883575	0.0
4	1.2838766913470561E-4	0.0	0.0	4.865635884867074	0.0
5	1.2838766913470561E-4	0.0	0.0	6.38279297103189	0.0
6	1.2838766913470561E-4	0.0	0.0	7.291264117829067	0.0
7	1.2838766913470561E-4	0.0	0.0	8.434941474481025	0.0
8	1.2838766913470561E-4	0.0	0.0	10.101670195187774	0.0
9	1.2838766913470561E-4	0.0	0.0	10.86634715255408	0.0
10	1.2838766913470561E-4	0.0	0.0	12.249981062818803	0.0
11	1.2838766913470561E-4	0.0	0.0	14.691914529760904	0.0
12	2.5677533826941123E-4	0.0	0.0	16.60026884377917	0.0
13	2.5677533826941123E-4	0.0	0.0	17.34376183573825	0.0
14	2.5677533826941123E-4	0.0	0.0	17.636742496703647	0.0
15	2.5677533826941123E-4	0.0	0.0	18.053617258384037	0.0
16	2.5677533826941123E-4	0.0	0.0	19.038350680647227	0.0
17	2.5677533826941123E-4	0.0	0.0	20.286150436967432	0.0
18	2.5677533826941123E-4	0.0	0.0	21.639741632654633	0.0
19	2.5677533826941123E-4	0.0	0.0	22.510595192395343	0.0
20	2.5677533826941123E-4	0.0	0.0	23.433702533473873	0.0
21	2.5677533826941123E-4	0.0	0.0	24.55658108772601	0.0
22	2.5677533826941123E-4	0.0	0.0	25.68857516648671	0.0
23	2.5677533826941123E-4	0.0	0.0	26.71606168257176	0.0
24	2.5677533826941123E-4	0.0	0.0	27.539925355409164	0.0
25	2.5677533826941123E-4	0.0	0.0	28.235401359111865	0.0
26	2.5677533826941123E-4	0.0	0.0	28.837282752015366	0.0
27	2.5677533826941123E-4	0.0	0.0	29.415155650790677	0.0
28	2.5677533826941123E-4	0.0	0.0	30.04990428699266	0.0
29	2.5677533826941123E-4	0.0	0.0	30.67938902876012	0.0
30	2.5677533826941123E-4	0.0	0.0	31.363695305248104	0.0
31	2.5677533826941123E-4	0.0	0.0	32.051596436471854	0.0
32	2.5677533826941123E-4	0.0	0.0	32.692764456130575	0.0
33	2.5677533826941123E-4	0.0	0.0	33.29888264211552	0.0
34	2.5677533826941123E-4	0.0	0.0	33.90127758569556	0.0
35	2.5677533826941123E-4	0.0	0.0	34.48081952416962	0.0
36	2.5677533826941123E-4	0.0	0.0	35.125967561571514	0.0
37	2.5677533826941123E-4	0.0	0.0	35.69793462756663	0.0
38	2.5677533826941123E-4	0.0	0.0	36.28620692754185	0.0
39	2.5677533826941123E-4	0.0	0.0	36.87935795894419	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCCG	30	2.1645974E-6	45.000004	29
CCGTACG	20	7.031884E-4	45.0	1
TCGTCAG	20	7.031884E-4	45.0	1
TCCGTTG	40	6.8102963E-9	45.0	1
CTAGGGA	780	0.0	42.98077	4
TAGGGCG	310	0.0	42.82258	5
TCTTGCG	175	0.0	42.428574	1
TCGTTAG	75	0.0	42.0	1
TTAGGGA	1365	0.0	41.868134	4
CTCGTAG	70	0.0	41.785713	1
CTTAGGG	1140	0.0	41.644737	3
GTTTTAG	395	0.0	41.58228	1
TAGGGAC	515	0.0	41.504852	5
CGTTTAT	360	0.0	41.25	1
TAGGGAT	705	0.0	41.17021	5
TAGGGAG	985	0.0	41.11675	5
TCTTAGG	670	0.0	40.97015	2
TTTAGGG	2595	0.0	40.92486	3
CGTTTTT	995	0.0	40.703518	1
TTGAGGG	1005	0.0	40.52239	3
>>END_MODULE