##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549687_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	557400
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30268927161823	33.0	31.0	34.0	31.0	34.0
2	32.47430032292788	34.0	31.0	34.0	31.0	34.0
3	32.52207212055974	34.0	31.0	34.0	31.0	34.0
4	36.014199856476495	37.0	35.0	37.0	35.0	37.0
5	35.16001973448152	37.0	35.0	37.0	35.0	37.0
6	26.279391819160388	37.0	0.0	37.0	0.0	37.0
7	31.1796035163258	37.0	19.0	37.0	17.0	37.0
8	34.724086831718694	37.0	32.0	37.0	32.0	37.0
9	37.220866523143165	39.0	37.0	39.0	34.0	39.0
10	37.36706315034087	39.0	37.0	39.0	34.0	39.0
11	37.420315751704344	39.0	37.0	39.0	35.0	39.0
12	37.15489594546107	39.0	37.0	39.0	34.0	39.0
13	37.13101363473269	39.0	37.0	39.0	33.0	39.0
14	38.29362755651238	40.0	38.0	41.0	34.0	41.0
15	38.43713670613563	40.0	38.0	41.0	34.0	41.0
16	38.51571941155364	40.0	38.0	41.0	35.0	41.0
17	38.518623968424826	40.0	38.0	41.0	35.0	41.0
18	38.50534266236096	40.0	38.0	41.0	35.0	41.0
19	38.48811984212415	40.0	37.0	41.0	35.0	41.0
20	38.399599928238246	40.0	37.0	41.0	34.0	41.0
21	38.249562253318985	40.0	37.0	41.0	34.0	41.0
22	38.22802116971654	40.0	37.0	41.0	34.0	41.0
23	38.179443846429855	40.0	36.0	41.0	34.0	41.0
24	38.115712235378545	40.0	36.0	41.0	34.0	41.0
25	37.99438284894151	40.0	36.0	41.0	34.0	41.0
26	37.90662540365985	40.0	36.0	41.0	34.0	41.0
27	37.893340509508434	40.0	36.0	41.0	34.0	41.0
28	37.80452278435594	40.0	36.0	41.0	34.0	41.0
29	37.7906799425906	40.0	36.0	41.0	34.0	41.0
30	37.536824542518836	40.0	36.0	41.0	33.0	41.0
31	37.53309293146753	40.0	35.0	41.0	33.0	41.0
32	37.4397380696089	39.0	35.0	41.0	33.0	41.0
33	37.34563150340868	39.0	35.0	41.0	33.0	41.0
34	37.230746322210265	39.0	35.0	41.0	33.0	41.0
35	37.17445102260495	39.0	35.0	41.0	33.0	41.0
36	37.06367958378184	39.0	35.0	41.0	32.0	41.0
37	36.97519196268389	39.0	35.0	41.0	32.0	41.0
38	36.90603157517043	39.0	35.0	41.0	32.0	41.0
39	36.85920882669537	39.0	35.0	41.0	32.0	41.0
40	36.76053462504485	39.0	35.0	41.0	31.0	41.0
41	36.68895048439182	39.0	35.0	41.0	31.0	41.0
42	36.65661284535343	39.0	35.0	41.0	31.0	41.0
43	36.553555794761394	39.0	35.0	41.0	31.0	41.0
44	36.36468066020811	39.0	35.0	40.0	31.0	41.0
45	36.34201471115895	39.0	35.0	40.0	31.0	41.0
46	36.26320236813778	39.0	35.0	40.0	31.0	41.0
47	36.20300861141012	38.0	35.0	40.0	30.0	41.0
48	36.19901506996771	38.0	35.0	40.0	31.0	41.0
49	36.18209364908504	38.0	35.0	40.0	31.0	41.0
50	36.099072479368495	38.0	35.0	40.0	30.0	41.0
51	35.10667025475421	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	9.0
10	5.0
11	10.0
12	6.0
13	9.0
14	5.0
15	14.0
16	18.0
17	35.0
18	90.0
19	157.0
20	287.0
21	495.0
22	853.0
23	1258.0
24	1931.0
25	3180.0
26	4350.0
27	5253.0
28	5869.0
29	6490.0
30	8137.0
31	10517.0
32	14476.0
33	23755.0
34	36205.0
35	49359.0
36	54053.0
37	88137.0
38	121638.0
39	120733.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.146035163257984	4.43308216720488	53.09651955507715	18.32436311445999
2	21.301758162899176	4.471115895227844	51.57391460351632	22.653211338356655
3	20.877646214567637	4.443667025475421	52.272515249372084	22.40617151058486
4	19.165052027269468	4.398815931108719	51.57427341227125	24.861858629350557
5	17.431108719052745	8.079834947972731	50.06996770721206	24.41908862576247
6	16.441155364190887	30.973806960889842	39.36257624686043	13.222461428058846
7	80.28525296017223	2.560100466451382	11.80265518478651	5.351991388589881
8	80.30803731611051	2.946896304269824	10.56189451022605	6.183171869393613
9	74.84858270541801	5.409041980624328	13.29529960531037	6.447075708647292
10	38.99982059562253	27.248654467168997	19.626300681736634	14.125224255471833
11	29.264800861141012	24.786508790814494	26.84553283100108	19.103157517043414
12	27.60566917832795	21.301219949766775	31.546645138141372	19.546465733763903
13	24.264083243631145	24.44492285611769	31.92447075708647	19.36652314316469
14	18.953534266236097	27.54790096878364	31.866702547542157	21.631862217438105
15	16.672407606745605	25.437926085396484	36.925008970218876	20.96465733763904
16	18.31539289558665	23.868675995694293	35.0821672048798	22.733763903839254
17	18.069608898457123	24.083243631144597	31.102439899533547	26.74470757086473
18	20.51345532831001	23.29978471474704	32.69178327951202	23.49497667743093
19	22.12091855041263	25.46968066020811	30.70093290276283	21.708467886616432
20	25.328130606386797	24.44420523860782	30.813957660566913	19.413706494438465
21	23.811266594904914	25.749551489056334	30.42070326515967	20.018478650879082
22	20.97470398277718	23.960351632579837	30.397021887334052	24.667922497308933
23	20.418909221385	25.791532113383564	28.513634732687475	25.275923932543954
24	22.16200215285253	23.080193756727667	30.850735557947612	23.90706853247219
25	20.025116612845352	24.94330821672049	29.42267671331181	25.608898457122354
26	18.897380696088984	29.020272694653748	28.090958019375673	23.991388589881595
27	19.544133476856835	27.01901686401148	29.590958019375673	23.845891639756008
28	17.918909221385	26.951740222461428	32.04718335127377	23.0821672048798
29	19.213850017940437	25.47362755651238	31.327771797631865	23.98475062791532
30	20.523322569070686	25.424291352709005	30.712773591675635	23.339612486544674
31	21.250089702188735	28.083602439899536	27.489056332974528	23.17725152493721
32	21.893254395407247	27.97416576964478	26.347506279153208	23.78507355579476
33	22.329565841406527	25.15572299964119	28.02439899533549	24.490312163616792
34	20.4183710082526	25.036239684248297	30.02996053103696	24.515428776462144
35	20.733225690706853	23.711876569788302	27.863114459992822	27.691783279512023
36	21.513096519555077	26.49120918550413	27.71636167922497	24.279332615715823
37	20.456763545030498	27.447972730534627	28.981700753498384	23.113562970936492
38	21.59903121636168	26.60351632579835	27.945461069250086	23.851991388589884
39	22.997667743092933	22.906350914962324	31.148008611410116	22.947972730534623
40	21.86562612127736	22.356835306781488	32.49425905992106	23.283279512020094
41	18.43595263724435	24.038930749910296	30.019016864011483	27.506099748833872
42	22.05633297452458	24.334947972730532	26.565482597775386	27.0432364549695
43	23.91334768568353	23.80301399354144	27.86562612127736	24.418012199497667
44	21.638858988159313	24.71761750986724	27.838894869034803	25.80462863293864
45	21.688554000717616	23.039468963042697	27.640653031933983	27.631324004305707
46	20.883387154646574	24.73232866881952	30.579296734840327	23.80498744169358
47	18.952816648726227	25.259777538571942	31.75905992106207	24.028345891639756
48	19.773053462504485	22.841585934696806	31.29045568711877	26.094904915679944
49	21.387334050950844	21.5	32.596878363832076	24.51578758521708
50	20.955866523143165	21.374955148905634	30.36993182633656	27.29924650161464
51	19.07552924291353	21.83925367778974	28.090958019375673	30.994259059921063
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	181.0
1	504.0
2	827.0
3	3498.0
4	6169.0
5	4014.5
6	1860.0
7	1885.0
8	1910.0
9	1956.5
10	2003.0
11	2024.5
12	2046.0
13	2083.5
14	2121.0
15	2110.0
16	2099.0
17	1964.0
18	1829.0
19	1867.0
20	1905.0
21	1892.0
22	1879.0
23	2050.5
24	2222.0
25	2674.0
26	3731.5
27	4337.0
28	4663.0
29	4989.0
30	5883.0
31	6777.0
32	8281.5
33	9786.0
34	10994.5
35	12203.0
36	13015.0
37	13827.0
38	16530.0
39	19233.0
40	22628.5
41	26024.0
42	30315.0
43	34606.0
44	38158.5
45	41711.0
46	48552.0
47	55393.0
48	62571.5
49	69750.0
50	65696.0
51	61642.0
52	50524.5
53	39407.0
54	33809.0
55	28211.0
56	26034.0
57	23857.0
58	22548.0
59	21239.0
60	19219.5
61	17200.0
62	14534.5
63	11869.0
64	10391.0
65	8913.0
66	7182.0
67	5451.0
68	4621.5
69	3792.0
70	3222.0
71	2652.0
72	2224.5
73	1797.0
74	1500.5
75	966.5
76	729.0
77	547.0
78	365.0
79	261.0
80	157.0
81	107.0
82	57.0
83	41.0
84	25.0
85	18.0
86	11.0
87	9.5
88	8.0
89	4.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	557400.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.76633115457044
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.39720690507717	25.17849814523803
2	12.014005707517057	8.593938132559089
3	4.622650367429264	4.960057315598153
4	2.4589168778232406	3.517857413351538
5	1.5460515183810526	2.7648295294221588
6	1.1049887970593437	2.3712837142628955
7	0.907159009743402	2.2712024686634296
8	0.7923969556550334	2.2672905537465113
9	0.6216072355623661	2.000934921168059
>10	5.365671986424532	37.061461079480786
>50	0.11496792027746322	2.7683632285431257
>100	0.04764436335820049	2.8641242207241393
>500	0.0036251146033413413	0.8850019741718901
>1k	0.003107241088578293	2.495157303070224
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGC	3019	0.5416218155723	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2834	0.50843200574094	No Hit
CTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGCT	2588	0.4642985288841048	No Hit
TCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGC	2006	0.3598851811984212	No Hit
GCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGC	1963	0.3521707929673484	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1061	0.1903480444922856	No Hit
TCCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTG	812	0.1456763545030499	No Hit
CCTGTATCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGC	785	0.140832436311446	No Hit
CTGTCACTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGCT	714	0.1280947255113025	No Hit
CGCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTG	701	0.12576246860423393	No Hit
GCTGTATCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGC	624	0.11194833153928956	No Hit
GCCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTG	607	0.1088984571223538	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.588087549336204E-4	0.0	0.0	0.48869752421959095	0.0
2	3.588087549336204E-4	0.0	0.0	1.8396124865446717	0.0
3	3.588087549336204E-4	0.0	0.0	2.6099748833871548	0.0
4	3.588087549336204E-4	0.0	0.0	3.3878722640832435	0.0
5	3.588087549336204E-4	0.0	0.0	4.638679583781844	0.0
6	3.588087549336204E-4	0.0	0.0	5.410118406889128	0.0
7	3.588087549336204E-4	0.0	0.0	6.336562612127736	0.0
8	3.588087549336204E-4	0.0	0.0	7.722640832436311	0.0
9	3.588087549336204E-4	0.0	0.0	8.39199856476498	0.0
10	3.588087549336204E-4	0.0	0.0	9.736634373878722	0.0
11	3.588087549336204E-4	0.0	0.0	12.321313240043057	0.0
12	3.588087549336204E-4	0.0	0.0	14.606925008970219	0.0
13	3.588087549336204E-4	0.0	0.0	15.360961607463222	0.0
14	3.588087549336204E-4	0.0	0.0	15.623071402942232	0.0
15	3.588087549336204E-4	0.0	0.0	16.00340868317187	0.0
16	3.588087549336204E-4	0.0	0.0	16.91047721564406	0.0
17	3.588087549336204E-4	0.0	0.0	18.259059921062075	0.0
18	5.382131324004305E-4	0.0	0.0	19.712055974165768	0.0
19	5.382131324004305E-4	0.0	0.0	20.628453534266235	0.0
20	5.382131324004305E-4	0.0	0.0	21.66935773232867	0.0
21	5.382131324004305E-4	0.0	0.0	22.95389307499103	0.0
22	5.382131324004305E-4	0.0	0.0	24.256907068532474	0.0
23	5.382131324004305E-4	0.0	0.0	25.522784355938285	0.0
24	5.382131324004305E-4	0.0	0.0	26.41388589881593	0.0
25	5.382131324004305E-4	0.0	0.0	27.235019734481522	0.0
26	5.382131324004305E-4	0.0	0.0	27.93146752780768	0.0
27	5.382131324004305E-4	0.0	0.0	28.60800143523502	0.0
28	5.382131324004305E-4	0.0	0.0	29.36437029063509	0.0
29	5.382131324004305E-4	0.0	0.0	30.08665231431647	0.0
30	5.382131324004305E-4	0.0	0.0	30.929852888410476	0.0
31	5.382131324004305E-4	0.0	0.0	31.742734122712594	0.0
32	5.382131324004305E-4	0.0	0.0	32.49372084678866	0.0
33	5.382131324004305E-4	0.0	0.0	33.216720487979906	0.0
34	5.382131324004305E-4	0.0	0.0	33.93236454969501	0.0
35	7.176175098672408E-4	0.0	0.0	34.68173663437388	0.0
36	7.176175098672408E-4	0.0	0.0	35.392357373519914	0.0
37	7.176175098672408E-4	0.0	0.0	36.074991029781124	0.0
38	7.176175098672408E-4	0.0	0.0	36.70685324721923	0.0
39	7.176175098672408E-4	0.0	0.0	37.40850376749193	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGGT	20	7.030158E-4	45.0	14
CACGGCA	20	7.030158E-4	45.0	45
ACGTTAG	20	7.030158E-4	45.0	1
TTAGCGA	20	7.030158E-4	45.0	17
CCGGCAC	20	7.030158E-4	45.0	41
CAACCCG	20	7.030158E-4	45.0	23
GCGCATT	20	7.030158E-4	45.0	18
ATTCCGA	25	3.8882106E-5	45.0	12
TACGATA	20	7.030158E-4	45.0	17
GGGCGTA	20	7.030158E-4	45.0	7
CGCAGTA	25	3.8882106E-5	45.0	37
AATTCCG	25	3.8882106E-5	45.0	11
GGCATAC	20	7.030158E-4	45.0	8
ACCGTAC	25	3.8882106E-5	45.0	16
CGGTCAT	20	7.030158E-4	45.0	24
CGACTGG	20	7.030158E-4	45.0	2
TGTACGA	20	7.030158E-4	45.0	15
CGATATC	20	7.030158E-4	45.0	19
TAGTACG	20	7.030158E-4	45.0	19
GTACGAT	20	7.030158E-4	45.0	16
>>END_MODULE