FastQCFastQC Report
Sat 18 Jun 2016
SRR3549686_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549686_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1435326
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGCT99750.6949640708800648No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82820.5770117729352078No Hit
CCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC78860.5494222218506458No Hit
TCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC78280.5453813280049271No Hit
GCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC54130.3771268687392272No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCC36250.2525558653574171No Hit
CGCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTG36080.2513714654371202No Hit
TCCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTG26050.1814918701396059No Hit
TTCCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCT24860.17320107069752794No Hit
TCTGTATCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC22880.15940629515524696No Hit
CCTGTATCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC22130.15418100138923144No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTC19160.13348883807580994No Hit
CGTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCT16490.1148867922687947No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTC15870.11056721608888852No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCT15650.10903446325085729No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGC15110.10527225173932611No Hit
TTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTG14850.10346081656710741No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGCA207.0338725E-445.00000411
GTTTACG406.8175723E-945.0000041
TTGTACG406.8175723E-945.0000041
GCCGAAG207.0338725E-445.0000041
TGCGACT207.0338725E-445.0000042
CGCTAAA207.0338725E-445.00000418
TATTCGA207.0338725E-445.00000443
CGATGCA207.0338725E-445.00000412
CGGTCGC207.0338725E-445.00000411
GATTCGA207.0338725E-445.00000441
GCGCTAA207.0338725E-445.00000417
TCGCGAG406.8175723E-945.0000041
CGGGTCG453.8562575E-1045.06
GCCGGCG351.2122109E-745.01
CCGTAGG750.045.02
TATCTCG253.891292E-545.01
TGTTACG351.2122109E-745.01
GTCCGAA253.891292E-545.09
GCCTATC700.045.02
TTACGAG253.891292E-545.01