##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549686_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1435326
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08156265545249	33.0	31.0	34.0	30.0	34.0
2	32.25860396871512	34.0	31.0	34.0	30.0	34.0
3	32.25090815605653	34.0	31.0	34.0	30.0	34.0
4	35.85397881735578	37.0	35.0	37.0	35.0	37.0
5	35.10168909362751	37.0	35.0	37.0	35.0	37.0
6	26.593410138184634	36.0	16.0	37.0	0.0	37.0
7	31.3428377943408	37.0	19.0	37.0	17.0	37.0
8	34.76715881966884	37.0	32.0	37.0	32.0	37.0
9	37.28756254676638	39.0	37.0	39.0	35.0	39.0
10	37.137907346484354	39.0	37.0	39.0	34.0	39.0
11	37.19292620631132	39.0	37.0	39.0	34.0	39.0
12	37.14533144386711	39.0	37.0	39.0	34.0	39.0
13	37.242828458482606	39.0	37.0	39.0	34.0	39.0
14	38.4133123764218	40.0	38.0	41.0	34.0	41.0
15	38.47585635597767	40.0	38.0	41.0	34.0	41.0
16	38.52957237589231	40.0	38.0	41.0	34.0	41.0
17	38.52520542371559	40.0	38.0	41.0	34.0	41.0
18	38.489063808500646	40.0	38.0	41.0	34.0	41.0
19	38.44852040581721	40.0	37.0	41.0	34.0	41.0
20	38.34188957769872	40.0	37.0	41.0	34.0	41.0
21	38.229225973750914	40.0	37.0	41.0	34.0	41.0
22	38.22489385686597	40.0	37.0	41.0	34.0	41.0
23	38.15832570440443	40.0	37.0	41.0	34.0	41.0
24	38.10615915826788	40.0	37.0	41.0	34.0	41.0
25	38.00821834203519	40.0	37.0	41.0	34.0	41.0
26	37.961433848477625	40.0	37.0	41.0	34.0	41.0
27	37.940637179288885	40.0	37.0	41.0	34.0	41.0
28	37.885963188850475	40.0	37.0	41.0	34.0	41.0
29	37.8566938799966	40.0	36.0	41.0	34.0	41.0
30	37.56543461206722	40.0	36.0	41.0	33.0	41.0
31	37.518855646731126	39.0	36.0	41.0	33.0	41.0
32	37.33527296237928	39.0	36.0	41.0	33.0	41.0
33	37.15353863860893	39.0	36.0	41.0	32.0	41.0
34	37.02228413614747	39.0	36.0	41.0	31.0	41.0
35	36.91606993811859	39.0	36.0	41.0	31.0	41.0
36	36.76997838818498	39.0	36.0	41.0	31.0	41.0
37	36.64913058078792	39.0	35.0	41.0	31.0	41.0
38	36.532174572187785	39.0	35.0	40.0	30.0	41.0
39	36.53705708668274	39.0	35.0	41.0	30.0	41.0
40	36.53847906329294	39.0	35.0	41.0	30.0	41.0
41	36.464890206127386	39.0	35.0	41.0	30.0	41.0
42	36.394249808057545	39.0	35.0	40.0	30.0	41.0
43	36.27095377635464	39.0	35.0	40.0	30.0	41.0
44	36.122143680251035	39.0	35.0	40.0	29.0	41.0
45	36.132513449906156	39.0	35.0	40.0	29.0	41.0
46	36.02739308003896	39.0	35.0	40.0	28.0	41.0
47	35.92207205889115	39.0	35.0	40.0	28.0	41.0
48	35.87096171880116	39.0	35.0	40.0	28.0	41.0
49	35.86931261608861	39.0	35.0	40.0	28.0	41.0
50	35.77325987267004	39.0	35.0	40.0	28.0	41.0
51	34.756998061764364	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	3.0
11	5.0
12	5.0
13	1.0
14	4.0
15	19.0
16	36.0
17	83.0
18	165.0
19	455.0
20	895.0
21	1581.0
22	2663.0
23	4551.0
24	7770.0
25	12695.0
26	16404.0
27	17163.0
28	16618.0
29	18062.0
30	21425.0
31	28003.0
32	37037.0
33	53668.0
34	78224.0
35	106862.0
36	146549.0
37	241329.0
38	331197.0
39	291782.0
40	68.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.289779464734842	4.118646216956984	59.54187411082918	14.049700207478999
2	23.750841272296327	3.487430730022308	52.28819097542997	20.47353702225139
3	17.372638689747138	3.3154140592450774	57.39337265541069	21.9185745955971
4	16.104494728026943	3.6542917776170714	56.88596179543881	23.35525169891718
5	15.223370857909632	6.6202381897910305	56.08196326130789	22.074427690991456
6	13.537900100743663	28.746849147859095	44.84904474662899	12.866206004768255
7	77.96848938847342	0.8922014929012642	15.589838127366187	5.5494709912591285
8	79.34956936612309	0.9843060043502312	12.974683103350737	6.691441526175936
9	75.24743507746672	2.618081188524419	16.09515886983166	6.039324864177197
10	33.934102775258026	21.52117358704573	26.781999350670162	17.76272428702608
11	21.156517752761395	25.88241277591293	30.30287196079497	22.65819751053071
12	18.042172997632594	21.57558631279584	37.38641953117271	22.995821158398858
13	20.22258358031555	23.93734942445131	38.42158506151216	17.41848193372098
14	19.724578249122498	25.449828122670393	35.77556596898545	19.05002765922167
15	15.97156325461951	26.948163692429457	36.61126461863019	20.46900843432084
16	16.411881342635752	25.848622542892695	34.18526522894451	23.55423088552705
17	16.504682559920187	27.030444651598312	31.63643660046568	24.82843618801582
18	17.875869314706204	24.60569933241647	35.78476248601363	21.733668866863695
19	17.8559435278118	29.024207740959195	33.41582330425283	19.70402542697617
20	18.818512310095407	28.28778967286874	33.745225823262444	19.1484721937734
21	18.381120386588133	29.165778366726443	33.88191950818142	18.571181738504006
22	16.8776291936466	26.490009935025217	31.987088647457096	24.645272223871093
23	16.533177828590855	27.383953192515147	29.92156485704293	26.161304121851064
24	17.661492929132475	26.218364329775955	31.950650932262075	24.169491808829495
25	17.00512636153738	27.229841861709463	29.437214960225067	26.32781681652809
26	16.99641753859402	27.82392292761366	32.84424583683428	22.335413696958042
27	18.13608894425378	25.913903879676113	34.03463742731616	21.91536974875394
28	17.079673885932532	24.421768991852723	34.42695248326861	24.071604638946134
29	17.764953745699582	23.468605738347943	34.831181209007575	23.935259306944904
30	20.482315515778296	24.915106393948133	33.95207778581312	20.65050030446045
31	18.31785949672757	29.588818150023062	29.649640569459486	22.443681783789884
32	18.053459632167186	31.89951272393867	27.649955480497113	22.397072163397024
33	18.951443783502842	26.91876270617267	30.055750400954206	24.074043109370276
34	18.35778074109993	26.52038630945165	28.85205172901487	26.269781220433547
35	19.70562785039775	26.237105716750065	28.06010620583756	25.997160227014632
36	22.020432988742627	25.751292737677712	31.578122322036943	20.650151951542718
37	20.133614245126193	27.54949049902252	31.3195747864945	20.997320469356787
38	18.347817847652728	31.832698634317218	28.92583287699101	20.893650641039038
39	19.208946260292088	26.507288239744835	32.41730450085904	21.86646099910404
40	19.051699753226792	25.33612573032189	30.398181319087094	25.21399319736422
41	18.582747055372785	27.993710139717386	27.008707429531686	26.414835375378136
42	24.254559591340225	26.00997961438725	27.062702131780515	22.672758662492004
43	23.72227633304211	24.362688337004972	30.228533448150458	21.68650188180246
44	20.70372863029026	23.94382878872117	28.24487259340387	27.1075699875847
45	22.258497372722292	22.304897981364512	28.245778310989976	27.19082633492322
46	22.223940763283046	23.04723804905645	31.589060603653806	23.139760584006698
47	19.20372096652607	25.850294636897818	32.03509167952089	22.91089271705522
48	18.72104316371333	25.629996251722602	32.457156074647855	23.191804509916214
49	23.047098707889358	22.2750093010229	32.7846774879017	21.893214503186037
50	20.70846622997145	21.372566232340247	30.054566001033912	27.86440153665439
51	18.737415750846846	21.0193363737576	27.68311867826543	32.56012919713013
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	135.0
1	365.0
2	595.0
3	8941.0
4	17287.0
5	11815.0
6	6343.0
7	6550.0
8	6757.0
9	7235.5
10	7714.0
11	8085.5
12	8457.0
13	8634.5
14	8812.0
15	8704.0
16	8596.0
17	8431.5
18	8267.0
19	8091.0
20	7915.0
21	8096.5
22	8278.0
23	8293.5
24	8309.0
25	8709.0
26	9926.5
27	10744.0
28	12192.5
29	13641.0
30	16086.5
31	18532.0
32	20908.0
33	23284.0
34	27326.0
35	31368.0
36	36483.5
37	41599.0
38	47336.0
39	53073.0
40	61235.5
41	69398.0
42	82632.0
43	95866.0
44	114661.0
45	133456.0
46	164341.5
47	195227.0
48	218906.5
49	242586.0
50	215761.0
51	188936.0
52	146173.5
53	103411.0
54	77587.5
55	51764.0
56	38560.5
57	25357.0
58	19442.5
59	13528.0
60	10850.5
61	8173.0
62	6109.5
63	4046.0
64	3066.0
65	2086.0
66	1557.0
67	1028.0
68	866.0
69	704.0
70	550.5
71	397.0
72	327.0
73	257.0
74	218.5
75	119.5
76	59.0
77	38.5
78	18.0
79	17.5
80	17.0
81	14.5
82	12.0
83	7.5
84	3.0
85	2.0
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1435326.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.97692765841118
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.01129533362966	32.38846820809144
2	13.91132512538854	12.513773275944764
3	5.309219314873399	7.163771191431007
4	2.5359594115482795	4.562386519914958
5	1.5196090052533497	3.4173672149175043
6	0.9561088193439685	2.5801702320721556
7	0.6706190671237415	2.1113669687863
8	0.5030337733875181	1.80999309083104
9	0.3777284466436351	1.5290158517293322
>10	2.0145429637054826	15.56852076977407
>50	0.11314469867901858	3.52932789229597
>100	0.06820621082578147	5.600018089556203
>500	0.0049449455246428605	1.4432008091025537
>1k	0.0034103072583743866	2.7621596524942915
>5k	8.525768145935966E-4	3.0204602330585066
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGCT	9975	0.6949640708800648	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8282	0.5770117729352078	No Hit
CCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC	7886	0.5494222218506458	No Hit
TCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC	7828	0.5453813280049271	No Hit
GCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC	5413	0.3771268687392272	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCC	3625	0.2525558653574171	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTG	3608	0.2513714654371202	No Hit
TCCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTG	2605	0.1814918701396059	No Hit
TTCCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCT	2486	0.17320107069752794	No Hit
TCTGTATCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC	2288	0.15940629515524696	No Hit
CCTGTATCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGC	2213	0.15418100138923144	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTC	1916	0.13348883807580994	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCT	1649	0.1148867922687947	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTC	1587	0.11056721608888852	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCT	1565	0.10903446325085729	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGC	1511	0.10527225173932611	No Hit
TTCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTG	1485	0.10346081656710741	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0901175064062103E-4	0.0	0.0	0.733491903581486	0.0
2	2.0901175064062103E-4	0.0	0.0	2.331038384311299	0.0
3	2.0901175064062103E-4	0.0	0.0	3.3218934235149367	0.0
4	2.0901175064062103E-4	0.0	0.0	4.554296375875585	0.0
5	2.0901175064062103E-4	0.0	0.0	6.4328243200499395	0.0
6	2.0901175064062103E-4	0.0	0.0	7.856194341912569	0.0
7	2.0901175064062103E-4	0.0	0.0	9.358988829018633	0.0
8	2.0901175064062103E-4	0.0	0.0	11.68919116632737	0.0
9	2.0901175064062103E-4	0.0	0.0	12.677398723356227	0.0
10	2.7868233418749467E-4	0.0	0.0	14.875157281342357	0.0
11	2.7868233418749467E-4	0.0	0.0	19.61268729194622	0.0
12	2.7868233418749467E-4	0.0	0.0	23.32201883056532	0.0
13	2.7868233418749467E-4	0.0	0.0	24.634124930503592	0.0
14	2.7868233418749467E-4	0.0	0.0	25.036611891653884	0.0
15	2.7868233418749467E-4	0.0	0.0	25.53273611709117	0.0
16	2.7868233418749467E-4	0.0	0.0	26.879050473550958	0.0
17	2.7868233418749467E-4	0.0	0.0	29.033125575653198	0.0
18	2.7868233418749467E-4	0.0	0.0	31.41042522743962	0.0
19	2.7868233418749467E-4	0.0	0.0	33.08969530266992	0.0
20	2.7868233418749467E-4	0.0	0.0	34.67121754918395	0.0
21	2.7868233418749467E-4	0.0	0.0	37.168350604671	0.0
22	3.483529177343684E-4	0.0	0.0	39.63085738013525	0.0
23	3.483529177343684E-4	0.0	0.0	42.29178597754099	0.0
24	3.483529177343684E-4	0.0	0.0	43.998018568603925	0.0
25	3.483529177343684E-4	0.0	0.0	45.4716907517874	0.0
26	3.483529177343684E-4	0.0	0.0	46.726736643800784	0.0
27	3.483529177343684E-4	0.0	0.0	47.940676891521505	0.0
28	3.483529177343684E-4	0.0	0.0	49.25821729697643	0.0
29	3.483529177343684E-4	0.0	0.0	50.604043959351394	0.0
30	3.483529177343684E-4	0.0	0.0	51.948755892389606	0.0
31	4.1802350128124205E-4	0.0	0.0	53.26845608593448	0.0
32	4.1802350128124205E-4	0.0	0.0	54.42742624323673	0.0
33	4.1802350128124205E-4	0.0	0.0	55.53351642762689	0.0
34	4.1802350128124205E-4	0.0	0.0	56.61292277851861	0.0
35	4.1802350128124205E-4	0.0	0.0	57.6705919073437	0.0
36	4.1802350128124205E-4	0.0	0.0	58.687294733043224	0.0
37	4.1802350128124205E-4	0.0	0.0	59.68783398335988	0.0
38	4.1802350128124205E-4	0.0	0.0	60.67987342248381	0.0
39	4.1802350128124205E-4	0.0	0.0	61.648294533785354	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGCA	20	7.0338725E-4	45.000004	11
GTTTACG	40	6.8175723E-9	45.000004	1
TTGTACG	40	6.8175723E-9	45.000004	1
GCCGAAG	20	7.0338725E-4	45.000004	1
TGCGACT	20	7.0338725E-4	45.000004	2
CGCTAAA	20	7.0338725E-4	45.000004	18
TATTCGA	20	7.0338725E-4	45.000004	43
CGATGCA	20	7.0338725E-4	45.000004	12
CGGTCGC	20	7.0338725E-4	45.000004	11
GATTCGA	20	7.0338725E-4	45.000004	41
GCGCTAA	20	7.0338725E-4	45.000004	17
TCGCGAG	40	6.8175723E-9	45.000004	1
CGGGTCG	45	3.8562575E-10	45.0	6
GCCGGCG	35	1.2122109E-7	45.0	1
CCGTAGG	75	0.0	45.0	2
TATCTCG	25	3.891292E-5	45.0	1
TGTTACG	35	1.2122109E-7	45.0	1
GTCCGAA	25	3.891292E-5	45.0	9
GCCTATC	70	0.0	45.0	2
TTACGAG	25	3.891292E-5	45.0	1
>>END_MODULE