SRR3549685_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549685_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	544542
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	2517	0.4622232995801977	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGCT	2219	0.40749841150912147	Illumina Single End Adapter 2 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	1845	0.3388168405742808	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1798	0.3301857340664265	No Hit
GCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	1738	0.3191673002266125	Illumina Single End Adapter 2 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTG	762	0.13993410976563792	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTATCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	739	0.1357103767937092	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTATCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	705	0.12946659761781462	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCACTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGCT	658	0.1208354911099603	Illumina Single End Adapter 2 (95% over 23bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	648	0.1189990854699913	No Hit
TCTGTATCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	628	0.1153262741900533	Illumina Single End Adapter 2 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	35	1.2103555E-7	45.000004	6
TCGTTCA	65	0.0	45.000004	16
TCGTTAG	85	0.0	45.000004	1
GCGCCAC	35	1.2103555E-7	45.000004	21
TCGATTG	20	7.030011E-4	45.000004	17
CGACACT	20	7.030011E-4	45.000004	38
CGTTCAT	65	0.0	45.000004	17
AGTATTC	20	7.030011E-4	45.000004	11
CGAGGGT	20	7.030011E-4	45.000004	4
TCTCGAG	20	7.030011E-4	45.000004	1
TATTAGG	65	0.0	45.000004	2
CGACTCA	20	7.030011E-4	45.000004	23
CCCGCTA	40	6.8030204E-9	45.000004	24
ATCGAGT	20	7.030011E-4	45.000004	41
CAACCCG	45	3.8380676E-10	45.0	23
AGCGGTC	25	3.8880928E-5	45.0	29
TAGGGCG	190	0.0	45.0	5
AGACACG	25	3.8880928E-5	45.0	24
CGAGCAT	25	3.8880928E-5	45.0	12
TACACGC	50	2.1827873E-11	45.0	35