##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549685_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	544542
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30952984342805	33.0	31.0	34.0	31.0	34.0
2	32.4814008836784	34.0	31.0	34.0	31.0	34.0
3	32.532811059569326	34.0	31.0	34.0	31.0	34.0
4	36.02383103598988	37.0	35.0	37.0	35.0	37.0
5	35.09511112090527	37.0	35.0	37.0	35.0	37.0
6	25.772724601591797	36.0	0.0	37.0	0.0	37.0
7	30.93127802814108	37.0	19.0	37.0	17.0	37.0
8	34.67123931671019	37.0	32.0	37.0	32.0	37.0
9	37.218802222785385	39.0	37.0	39.0	34.0	39.0
10	37.337882110103536	39.0	37.0	39.0	34.0	39.0
11	37.429226763041235	39.0	37.0	39.0	35.0	39.0
12	37.15855342654928	39.0	37.0	39.0	34.0	39.0
13	37.11923230898627	39.0	37.0	39.0	33.0	39.0
14	38.30156718857315	40.0	38.0	41.0	34.0	41.0
15	38.43168938300443	40.0	38.0	41.0	34.0	41.0
16	38.5390768756129	40.0	38.0	41.0	35.0	41.0
17	38.52690517903119	40.0	38.0	41.0	35.0	41.0
18	38.519030671647	40.0	38.0	41.0	35.0	41.0
19	38.50199984574193	40.0	37.0	41.0	34.0	41.0
20	38.42903761326032	40.0	37.0	41.0	34.0	41.0
21	38.31541919631544	40.0	37.0	41.0	34.0	41.0
22	38.29498734716514	40.0	37.0	41.0	34.0	41.0
23	38.24894131214856	40.0	37.0	41.0	34.0	41.0
24	38.20033900048114	40.0	37.0	41.0	34.0	41.0
25	38.06690576668099	40.0	36.0	41.0	34.0	41.0
26	38.004383500262605	40.0	36.0	41.0	34.0	41.0
27	37.981336609481	40.0	36.0	41.0	34.0	41.0
28	37.904345303025295	40.0	36.0	41.0	34.0	41.0
29	37.885588255818654	40.0	36.0	41.0	34.0	41.0
30	37.66648302610267	40.0	36.0	41.0	33.0	41.0
31	37.65093050673777	40.0	36.0	41.0	33.0	41.0
32	37.58154191963154	40.0	36.0	41.0	33.0	41.0
33	37.46719077683631	40.0	35.0	41.0	33.0	41.0
34	37.38791681817013	40.0	35.0	41.0	33.0	41.0
35	37.391402316074796	40.0	35.0	41.0	33.0	41.0
36	37.297822757473256	40.0	35.0	41.0	33.0	41.0
37	37.20751200091086	39.0	35.0	41.0	33.0	41.0
38	37.16175795439103	39.0	35.0	41.0	32.0	41.0
39	37.10576411002273	39.0	35.0	41.0	32.0	41.0
40	36.97976280984754	39.0	35.0	41.0	32.0	41.0
41	36.86778981235607	39.0	35.0	41.0	31.0	41.0
42	36.82774147815963	39.0	35.0	41.0	31.0	41.0
43	36.737731892122184	39.0	35.0	41.0	31.0	41.0
44	36.61238435235482	39.0	35.0	41.0	31.0	41.0
45	36.618057009376685	39.0	35.0	41.0	31.0	41.0
46	36.51528991335838	39.0	35.0	40.0	31.0	41.0
47	36.419776619617956	39.0	35.0	40.0	31.0	41.0
48	36.4292249266356	39.0	35.0	40.0	31.0	41.0
49	36.39047125841533	39.0	35.0	40.0	31.0	41.0
50	36.30071325995056	39.0	35.0	40.0	31.0	41.0
51	35.221336462568544	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	18.0
11	11.0
12	8.0
13	5.0
14	5.0
15	9.0
16	8.0
17	37.0
18	72.0
19	144.0
20	254.0
21	497.0
22	718.0
23	1144.0
24	1657.0
25	2636.0
26	3520.0
27	4423.0
28	5108.0
29	5955.0
30	7455.0
31	10237.0
32	14285.0
33	22965.0
34	35228.0
35	48359.0
36	52630.0
37	87243.0
38	119828.0
39	120004.0
40	75.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.540623129161755	4.513150500787818	53.16118866864264	17.785037701407788
2	20.23204821666648	4.336120997094806	52.86516007947964	22.566670706759073
3	20.624304461363863	4.508926767815889	52.36767779161203	22.499090979208216
4	19.60730301794903	4.974455597548031	51.49924156447069	23.918999820032248
5	17.884754527658107	8.167965005454125	49.044701786088126	24.902578680799646
6	15.690066147331153	31.837213658450587	39.087894046740196	13.384826147478062
7	81.44973206841712	2.1124174076563427	11.21033822919077	5.227512294735759
8	82.109368974294	2.813557080996507	8.999122198104095	6.077951746605404
9	76.78783271079183	4.930565502752772	11.792295176496946	6.489306609958461
10	39.2120350680021	26.914911981077676	18.821321404042298	15.051731546877928
11	30.11980710395158	25.401162812051226	25.39198078385138	19.08704930014581
12	29.466413977250603	20.72163396028222	30.40647002435074	19.405482038116435
13	24.47469616668687	24.714898024394813	30.591579712859613	20.218826096058706
14	18.56734650403458	28.115186707361417	30.109706872931746	23.207759915672252
15	15.897029062955658	25.71482089535793	37.12257273084537	21.26557731084104
16	18.557613554142748	23.5425366638386	35.14054012362683	22.759309658391825
17	18.045807302283386	23.693489207444056	30.591946993987605	27.668756496284953
18	20.043632998005663	23.168093553848923	32.15252450683327	24.635748941312148
19	22.666938454701384	25.87018081249931	29.59165684189649	21.87122389090281
20	26.421653426182	25.112847126576092	29.2890171924296	19.176482254812303
21	24.307399612885693	26.334424158283475	28.617994571584926	20.740181657245905
22	20.57582335246868	24.675415303135477	29.205460735811013	25.54330060858483
23	21.234909336653555	26.341586140279354	27.2452813557081	25.178223167358993
24	22.289740736251748	23.39562421264108	29.782459387889272	24.5321756632179
25	20.650748702579417	25.0298415916495	27.648923315373285	26.6704863903978
26	20.055385994101467	28.63121669219271	27.43663482339287	23.87676249031296
27	21.93843633732568	28.581633739913542	27.727705117327954	21.752224805432824
28	18.499215854791736	27.707504655288297	30.47000965949367	23.3232698304263
29	20.088441295620907	25.488943001641744	29.249350830606268	25.173264872131075
30	23.73462469377936	26.355359182579118	27.136749782385934	22.77326634125559
31	22.502763790488153	30.69203110136592	23.719566167531614	23.085638940614313
32	24.245880023946732	29.788335885937173	23.373403704397457	22.59238038571864
33	24.71912175736674	28.551149406290055	24.170954673835997	22.55877416250721
34	21.223707262249743	28.45253442342372	26.178329678886108	24.145428635440425
35	22.433347657297325	26.606212192998886	24.248450991842684	26.711989157861098
36	24.220904907243153	29.91523151565903	23.971888302463356	21.891975274634465
37	21.908135644266192	30.405919102658746	26.59482647803108	21.09111877504398
38	21.927417903485864	28.63948051757256	24.23926896364284	25.193832615298728
39	23.390665917413163	25.588843468456062	26.96504585504883	24.055444759081944
40	25.10862339360417	25.45735682463428	27.644883222965355	21.789136558796198
41	20.8055576980288	28.020979098031006	26.685177635517555	24.488285568422636
42	23.616543811129354	27.571977919058583	25.01313030032578	23.798347969486283
43	23.10896129224192	27.074862912318977	26.023337042872726	23.79283875256638
44	21.12197038979546	26.564893066099582	26.01764418538882	26.29549235871613
45	22.47393222194064	24.78192683025368	25.01129389468581	27.73284705311987
46	23.15413687098516	26.107628061747302	26.8838767257622	23.85435834150534
47	20.486757678930186	27.389439198445665	28.71495678937529	23.40884633324886
48	21.664995537534296	24.539888566905766	28.646091577876454	25.149024317683484
49	22.045131505007877	23.841687142589553	29.674295095695097	24.43888625670747
50	21.38971833210294	23.65565925126069	27.585200039666365	27.369422376970004
51	19.93363230017152	24.109435084897033	25.582416048716166	30.374516566215277
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	141.0
1	438.5
2	736.0
3	2373.0
4	4010.0
5	2688.0
6	1366.0
7	1316.5
8	1267.0
9	1332.0
10	1397.0
11	1487.5
12	1578.0
13	1530.5
14	1483.0
15	1412.5
16	1342.0
17	1205.0
18	1068.0
19	1130.0
20	1192.0
21	1455.5
22	1719.0
23	1871.0
24	2023.0
25	2385.0
26	3381.0
27	4015.0
28	4802.5
29	5590.0
30	6398.0
31	7206.0
32	8495.0
33	9784.0
34	10553.5
35	11323.0
36	12680.0
37	14037.0
38	15436.5
39	16836.0
40	20399.0
41	23962.0
42	28076.0
43	32190.0
44	36845.0
45	41500.0
46	48267.0
47	55034.0
48	61774.0
49	68514.0
50	63913.5
51	59313.0
52	50414.5
53	41516.0
54	35144.5
55	28773.0
56	26352.0
57	23931.0
58	22971.0
59	22011.0
60	20610.0
61	19209.0
62	16324.5
63	13440.0
64	11023.5
65	8607.0
66	6928.5
67	5250.0
68	4596.0
69	3942.0
70	3332.0
71	2722.0
72	2150.5
73	1579.0
74	1375.5
75	835.0
76	498.0
77	388.5
78	279.0
79	205.5
80	132.0
81	101.0
82	70.0
83	45.5
84	21.0
85	14.0
86	7.0
87	4.5
88	2.0
89	4.0
90	6.0
91	4.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	544542.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.167599424963505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.05141377426845	25.33591472540112
2	12.05401734631795	8.719297416863784
3	4.67796227198701	5.07571996734955
4	2.437156541918331	3.525844061761259
5	1.6363668942083975	2.9591731171000473
6	1.1474718533500972	2.490078140603207
7	0.9362950711606542	2.3704481554114367
8	0.7595187617075531	2.1975976263346473
9	0.6463996793407663	2.104085220373952
>10	5.484097697636106	36.82281834960107
>50	0.12145442537948808	3.018612900326032
>100	0.04206213865955032	2.6692874689345345
>500	0.0031546603994662744	0.7872658061828719
>1k	0.002628883666221895	1.9238570437565494
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	2517	0.4622232995801977	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGCT	2219	0.40749841150912147	Illumina Single End Adapter 2 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	1845	0.3388168405742808	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1798	0.3301857340664265	No Hit
GCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	1738	0.3191673002266125	Illumina Single End Adapter 2 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTG	762	0.13993410976563792	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTATCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	739	0.1357103767937092	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTATCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	705	0.12946659761781462	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCACTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGCT	658	0.1208354911099603	Illumina Single End Adapter 2 (95% over 23bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	648	0.1189990854699913	No Hit
TCTGTATCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	628	0.1153262741900533	Illumina Single End Adapter 2 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4732417334200117	0.0
2	0.0	0.0	0.0	1.8248362844371968	0.0
3	0.0	0.0	0.0	2.549114668840971	0.0
4	0.0	0.0	0.0	3.299470013332305	0.0
5	0.0	0.0	0.0	4.4696276871205525	0.0
6	0.0	0.0	0.0	5.201986256340191	0.0
7	0.0	0.0	0.0	6.18776880387555	0.0
8	0.0	0.0	0.0	7.6879285711662275	0.0
9	0.0	0.0	0.0	8.354176537346982	0.0
10	0.0	0.0	0.0	9.72927708055577	0.0
11	0.0	0.0	0.0	12.294919400156461	0.0
12	0.0	0.0	0.0	14.645518619316784	0.0
13	0.0	0.0	0.0	15.403586867495987	0.0
14	0.0	0.0	0.0	15.66527467119157	0.0
15	0.0	0.0	0.0	16.05404174517301	0.0
16	0.0	0.0	0.0	16.95204410311785	0.0
17	0.0	0.0	0.0	18.187210536561	0.0
18	0.0	0.0	0.0	19.58104241729747	0.0
19	0.0	0.0	0.0	20.48051389975429	0.0
20	0.0	0.0	0.0	21.43599575423016	0.0
21	0.0	0.0	0.0	22.753800441471917	0.0
22	0.0	0.0	0.0	24.05985213261787	0.0
23	0.0	0.0	0.0	25.329726632656435	0.0
24	0.0	0.0	0.0	26.25527507520081	0.0
25	0.0	0.0	0.0	27.107918213838417	0.0
26	0.0	0.0	0.0	27.811628855074538	0.0
27	0.0	0.0	0.0	28.540498253578235	0.0
28	0.0	0.0	0.0	29.236312350562493	0.0
29	0.0	0.0	0.0	30.01586654472933	0.0
30	0.0	0.0	0.0	30.90340139052635	0.0
31	0.0	0.0	0.0	31.720601900312555	0.0
32	0.0	0.0	0.0	32.49923789165941	0.0
33	0.0	0.0	0.0	33.23655475610697	0.0
34	0.0	0.0	0.0	33.96983152814659	0.0
35	0.0	0.0	0.0	34.72000323207393	0.0
36	0.0	0.0	0.0	35.47054221712926	0.0
37	0.0	0.0	0.0	36.24715816227215	0.0
38	0.0	0.0	0.0	36.94444138376838	0.0
39	0.0	0.0	0.0	37.689838433031795	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	35	1.2103555E-7	45.000004	6
TCGTTCA	65	0.0	45.000004	16
TCGTTAG	85	0.0	45.000004	1
GCGCCAC	35	1.2103555E-7	45.000004	21
TCGATTG	20	7.030011E-4	45.000004	17
CGACACT	20	7.030011E-4	45.000004	38
CGTTCAT	65	0.0	45.000004	17
AGTATTC	20	7.030011E-4	45.000004	11
CGAGGGT	20	7.030011E-4	45.000004	4
TCTCGAG	20	7.030011E-4	45.000004	1
TATTAGG	65	0.0	45.000004	2
CGACTCA	20	7.030011E-4	45.000004	23
CCCGCTA	40	6.8030204E-9	45.000004	24
ATCGAGT	20	7.030011E-4	45.000004	41
CAACCCG	45	3.8380676E-10	45.0	23
AGCGGTC	25	3.8880928E-5	45.0	29
TAGGGCG	190	0.0	45.0	5
AGACACG	25	3.8880928E-5	45.0	24
CGAGCAT	25	3.8880928E-5	45.0	12
TACACGC	50	2.1827873E-11	45.0	35
>>END_MODULE