##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549679_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1883411
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25666251285566	33.0	31.0	34.0	30.0	34.0
2	32.413734442455734	34.0	31.0	34.0	30.0	34.0
3	32.468825975849136	34.0	31.0	34.0	30.0	34.0
4	35.979030068317535	37.0	35.0	37.0	35.0	37.0
5	35.08326700863486	37.0	35.0	37.0	35.0	37.0
6	25.81183554731283	36.0	0.0	37.0	0.0	37.0
7	30.96704065124394	37.0	19.0	37.0	17.0	37.0
8	34.69523540002687	37.0	32.0	37.0	32.0	37.0
9	37.27513219366352	39.0	37.0	39.0	35.0	39.0
10	37.183213860384164	39.0	37.0	39.0	34.0	39.0
11	37.2545514494712	39.0	37.0	39.0	34.0	39.0
12	37.1448319033923	39.0	37.0	39.0	34.0	39.0
13	37.22178642898443	39.0	37.0	39.0	34.0	39.0
14	38.40690375069488	40.0	38.0	41.0	34.0	41.0
15	38.48143873004883	40.0	38.0	41.0	34.0	41.0
16	38.54626526021139	40.0	38.0	41.0	34.0	41.0
17	38.524493060728645	40.0	38.0	41.0	34.0	41.0
18	38.517867847219755	40.0	38.0	41.0	34.0	41.0
19	38.52643687437315	40.0	38.0	41.0	34.0	41.0
20	38.45854462992942	40.0	38.0	41.0	34.0	41.0
21	38.38558339098582	40.0	38.0	41.0	34.0	41.0
22	38.381218969200035	40.0	37.0	41.0	34.0	41.0
23	38.323738153807106	40.0	37.0	41.0	34.0	41.0
24	38.25857712416462	40.0	37.0	41.0	34.0	41.0
25	38.15756783835286	40.0	37.0	41.0	34.0	41.0
26	38.1430776394531	40.0	37.0	41.0	34.0	41.0
27	38.12105429988463	40.0	37.0	41.0	34.0	41.0
28	38.03329915775155	40.0	37.0	41.0	34.0	41.0
29	38.046819308159506	40.0	37.0	41.0	34.0	41.0
30	37.810405163822445	40.0	37.0	41.0	33.0	41.0
31	37.83122430526316	40.0	37.0	41.0	33.0	41.0
32	37.772454870445166	40.0	37.0	41.0	33.0	41.0
33	37.70391858176468	40.0	37.0	41.0	33.0	41.0
34	37.63093663571042	40.0	37.0	41.0	33.0	41.0
35	37.6339211144036	40.0	37.0	41.0	33.0	41.0
36	37.54171500538119	40.0	37.0	41.0	33.0	41.0
37	37.432911350735445	40.0	36.0	41.0	33.0	41.0
38	37.3354392641861	40.0	36.0	41.0	32.0	41.0
39	37.32100587710277	40.0	36.0	41.0	32.0	41.0
40	37.312121464725436	40.0	36.0	41.0	32.0	41.0
41	37.21455274499299	40.0	36.0	41.0	32.0	41.0
42	37.13017020714013	39.0	36.0	41.0	32.0	41.0
43	37.00357011825884	39.0	35.0	41.0	31.0	41.0
44	36.86859639239656	39.0	35.0	41.0	31.0	41.0
45	36.8485869520779	39.0	35.0	41.0	31.0	41.0
46	36.73456085793276	39.0	35.0	41.0	31.0	41.0
47	36.594425751999964	39.0	35.0	40.0	31.0	41.0
48	36.553517527507275	39.0	35.0	40.0	31.0	41.0
49	36.519896613113126	39.0	35.0	40.0	31.0	41.0
50	36.43109124880337	39.0	35.0	40.0	31.0	41.0
51	35.29794452724339	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	13.0
10	7.0
11	7.0
12	3.0
13	6.0
14	7.0
15	19.0
16	39.0
17	85.0
18	220.0
19	437.0
20	866.0
21	1466.0
22	2459.0
23	3751.0
24	5996.0
25	9061.0
26	12191.0
27	14160.0
28	16519.0
29	20037.0
30	25958.0
31	34755.0
32	47929.0
33	72858.0
34	107572.0
35	143232.0
36	186257.0
37	315833.0
38	434433.0
39	427019.0
40	215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.144172461560434	4.202109895291044	57.8208898641879	13.832827778960619
2	19.951407313645294	5.065277838984693	53.629929951561294	21.353384895808723
3	19.37564344691626	4.997103659265025	53.392116749875626	22.23513614394309
4	18.161728905692915	4.054133696787372	53.478714948569376	24.305422448950335
5	17.72687958178008	7.660675232331126	51.34375874410843	23.268686441780364
6	14.717658546116594	31.940983672708718	40.99190245782785	12.349455323346842
7	83.4637792813146	1.5330164260482708	10.598961140186608	4.404243152450527
8	85.17062924661691	2.4061131638288193	7.537282090844749	4.885975498709523
9	80.6186222762849	3.455326532551843	10.025055603901645	5.900995587261622
10	38.37691295208534	21.73195335484395	22.2151723654582	17.67596132761251
11	26.785656449919852	27.058831025198433	24.51705973895236	21.63845278592936
12	22.733593464198734	22.498010259045955	32.24362605931472	22.52477021744059
13	22.81344857813828	23.89945688965393	34.2431365219806	19.04395801022719
14	21.39087007562343	25.652446545124775	32.249891287669016	20.70679209158277
15	19.209721085838407	26.538020644458378	33.666629323073934	20.58562894662928
16	19.89257788130153	25.428597369347422	31.483091051289392	23.195733698061655
17	20.036996704383693	26.373478757424696	29.623964179884265	23.965560358307346
18	20.913066770874757	24.734272020286596	32.3434980468947	22.00916316194394
19	21.299068551686275	27.850479794373083	29.633308927260167	21.217142726680475
20	21.94422778671251	27.878779512278516	30.56353605240704	19.613456648601925
21	22.261789912026636	27.920034448136917	30.310112874991173	19.50806276484527
22	20.99175379139232	25.16131635633433	29.963136033505165	23.883793818768183
23	19.817926092605383	26.714137275400855	28.991335401566626	24.476601230427132
24	20.905474163631833	25.642146084949065	29.62401727503981	23.82836247637929
25	19.14319285594063	27.45417755338585	27.96920056217151	25.433429028502008
26	18.863540671685573	27.04046010137989	29.92363323777975	24.172365989154784
27	20.564709455344584	26.550710386633614	29.644405814769055	23.240174343252747
28	17.88738623699235	26.278969380554752	31.417677819658056	24.415966562794843
29	18.81203837080701	24.134031286851357	31.697701670001926	25.35622867233971
30	21.70179530649444	25.406403594329653	30.85662131101496	22.03517978816095
31	21.380888186380986	27.525431252127124	28.118238663786077	22.97544189770581
32	20.586531564273542	27.86784191023627	28.5391239617906	23.006502563699584
33	21.67853962836577	26.26097012282502	28.480029053669114	23.580461195140096
34	20.167239120935367	26.138107932894094	28.744071262193966	24.950581683976573
35	20.30103891290855	24.76910244232406	28.427252469057475	26.50260617570992
36	21.97804940079462	27.248433825649315	27.36253531491533	23.41098145864073
37	21.10166076337029	28.60060815191161	29.08823406043609	21.209497024282005
38	19.694320570496828	28.641916182925552	30.073043005483136	21.590720241094484
39	20.55589565952413	25.329415618789525	32.2584927028673	21.856196018819045
40	23.18930918424072	23.484730629692617	30.415984615147728	22.909975570918935
41	22.730460850021583	25.435659025034894	26.595310317291336	25.238569807652176
42	22.60239533484725	26.110817022944012	26.874166074213225	24.412621567995515
43	20.853334720886732	26.385743738355565	28.689064681049437	24.071856859708262
44	19.637243278286046	24.644541207415692	28.854084424483023	26.864131089815235
45	21.607179739313406	22.403978738575915	28.595139350890484	27.3937021712202
46	23.459988287208684	23.40434456419762	29.27937662039778	23.856290528195917
47	20.356151684364168	25.19800510881587	30.566350095650925	23.879493111169044
48	20.39809685724465	24.961519286018824	30.733440550150764	23.906943306585767
49	21.947307305734117	22.75615890530532	32.30245549165849	22.994078297302075
50	22.37551973520384	21.506245848622527	30.29880360686011	25.819430809313527
51	21.162136145535946	21.244805302719374	28.403837505462164	29.189221046282515
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	155.0
1	613.5
2	1072.0
3	5011.5
4	8951.0
5	6058.5
6	3166.0
7	3160.0
8	3154.0
9	3323.0
10	3492.0
11	3604.5
12	3717.0
13	3775.0
14	3833.0
15	3825.5
16	3818.0
17	3931.5
18	4045.0
19	4140.0
20	4235.0
21	4825.0
22	5415.0
23	6278.5
24	7142.0
25	8792.5
26	12566.0
27	14689.0
28	17098.0
29	19507.0
30	23284.0
31	27061.0
32	31799.0
33	36537.0
34	42127.0
35	47717.0
36	54256.0
37	60795.0
38	68163.0
39	75531.0
40	85737.0
41	95943.0
42	108441.5
43	120940.0
44	140634.0
45	160328.0
46	192795.5
47	225263.0
48	240958.5
49	256654.0
50	239798.5
51	222943.0
52	186589.0
53	150235.0
54	124735.5
55	99236.0
56	82894.0
57	66552.0
58	56628.0
59	46704.0
60	40754.5
61	34805.0
62	28826.0
63	22847.0
64	18593.0
65	14339.0
66	11430.5
67	8522.0
68	7178.0
69	5834.0
70	4664.5
71	3495.0
72	2746.5
73	1998.0
74	1538.0
75	829.5
76	581.0
77	449.5
78	318.0
79	296.0
80	274.0
81	152.0
82	30.0
83	18.5
84	7.0
85	6.0
86	5.0
87	3.5
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1883411.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.00424598911335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.34004501268709	26.352161275583523
2	13.873894045994795	10.545337643017625
3	5.347561375896719	6.096901139143811
4	2.738703542317333	4.163294524539361
5	1.5528697934777076	2.9507822810195226
6	0.9942456176167591	2.267133301530315
7	0.729594804844153	1.9409390304973437
8	0.5725170818657134	1.7406464009755138
9	0.444251775168346	1.519510837013829
>10	4.241057130346614	31.745235076038213
>50	0.10374461704410122	2.678141961267931
>100	0.05549059737072644	3.781197255024556
>500	0.003012302684956948	0.8184135838935377
>1k	0.0025366759452269036	2.278398826573675
>5k	4.756267397300444E-4	1.1219068638811507
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGCT	6707	0.356109208239731	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	6621	0.3515430248628685	No Hit
TCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	5292	0.2809795631436792	No Hit
GCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	4904	0.260378642792253	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGC	4866	0.2583610268815463	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCG	4075	0.21636275884552017	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3625	0.19246993885030936	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTC	3354	0.17808115169763797	No Hit
TCCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTG	2060	0.10937602042252062	No Hit
CCTGTATCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	1976	0.10491602735674793	No Hit
TCTGTATCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	1920	0.10194269864623282	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5928546663473877E-4	0.0	0.0	0.38180726352346883	0.0
2	1.5928546663473877E-4	0.0	0.0	1.3662976376372444	0.0
3	1.5928546663473877E-4	0.0	0.0	1.9222570113480275	0.0
4	1.5928546663473877E-4	0.0	0.0	2.6096799901880154	0.0
5	1.5928546663473877E-4	0.0	0.0	3.825187386077707	0.0
6	1.5928546663473877E-4	0.0	0.0	4.563475523929721	0.0
7	1.5928546663473877E-4	0.0	0.0	5.468960306592666	0.0
8	1.5928546663473877E-4	0.0	0.0	6.982437715400409	0.0
9	1.5928546663473877E-4	0.0	0.0	7.563245621906212	0.0
10	1.5928546663473877E-4	0.0	0.0	9.09461609813259	0.0
11	1.5928546663473877E-4	0.0	0.0	11.832202318028301	0.0
12	1.5928546663473877E-4	0.0	0.0	14.30208276366656	0.0
13	2.123806221796517E-4	0.0	0.0	15.07339608826751	0.0
14	2.123806221796517E-4	0.0	0.0	15.314979045996864	0.0
15	2.123806221796517E-4	0.0	0.0	15.706396532673962	0.0
16	2.123806221796517E-4	0.0	0.0	16.649897446707065	0.0
17	2.123806221796517E-4	0.0	0.0	18.0601578731355	0.0
18	2.123806221796517E-4	0.0	0.0	19.545016993104532	0.0
19	2.123806221796517E-4	0.0	0.0	20.709181373582293	0.0
20	2.123806221796517E-4	0.0	0.0	21.80772014180654	0.0
21	2.123806221796517E-4	0.0	0.0	23.52917127488371	0.0
22	2.123806221796517E-4	0.0	0.0	25.267081906179797	0.0
23	2.123806221796517E-4	0.0	0.0	27.048052708622812	0.0
24	2.123806221796517E-4	0.0	0.0	28.334123566231693	0.0
25	2.123806221796517E-4	0.0	0.0	29.483633683779058	0.0
26	2.6547577772456464E-4	0.0	0.0	30.441098623720475	0.0
27	2.6547577772456464E-4	0.0	0.0	31.400156418328237	0.0
28	2.6547577772456464E-4	0.0	0.0	32.43567123692067	0.0
29	2.6547577772456464E-4	0.0	0.0	33.505804096928394	0.0
30	2.6547577772456464E-4	0.0	0.0	34.596272401509815	0.0
31	2.6547577772456464E-4	0.0	0.0	35.649839572987524	0.0
32	3.1857093326947755E-4	0.0	0.0	36.689124147623644	0.0
33	3.1857093326947755E-4	0.0	0.0	37.68046379680272	0.0
34	3.1857093326947755E-4	0.0	0.0	38.60601854826164	0.0
35	3.1857093326947755E-4	0.0	0.0	39.57091680997934	0.0
36	3.1857093326947755E-4	0.0	0.0	40.516169864145425	0.0
37	3.1857093326947755E-4	0.0	0.0	41.46641386293273	0.0
38	3.1857093326947755E-4	0.0	0.0	42.41963119043056	0.0
39	3.1857093326947755E-4	0.0	0.0	43.36307900930811	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGCG	25	3.8917577E-5	45.0	1
TAGGGCG	950	0.0	44.05263	5
CGTTTAT	930	0.0	43.064518	1
TTAGGGA	3690	0.0	42.073174	4
GCGATAT	75	0.0	42.0	9
AGGGCGA	1215	0.0	41.296295	6
TTAGGGT	2315	0.0	41.015118	4
TAGGGAT	1660	0.0	40.933735	5
TATTGCG	110	0.0	40.909092	1
TCGCTAG	165	0.0	40.90909	1
GTTAGGG	2775	0.0	40.864864	3
TTTAGGG	7820	0.0	40.597828	3
CTAGGGT	1140	0.0	40.46052	4
TTCGTAG	380	0.0	40.263157	1
AGGGCGC	190	0.0	40.263157	6
CTTAGGG	3600	0.0	40.187504	3
TAGGGTC	1195	0.0	40.104603	5
CGTTTTT	2615	0.0	40.00956	1
TTCGCAG	180	0.0	40.000004	1
TAGGGTA	1370	0.0	39.908756	5
>>END_MODULE