FastQCFastQC Report
Sat 18 Jun 2016
SRR3549677_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549677_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959633
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC35010.36482697031052497TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGCT29960.31220268581843263TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGC20400.2125812680472639No Hit
GCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC18030.18788432661236118TruSeq Adapter, Index 15 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC17130.17850574125733484TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16980.17694264369816376No Hit
GAATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTC13920.14505545349107418No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCG12810.13348853155320836No Hit
CCTGTATCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC11360.11837858848122147TruSeq Adapter, Index 21 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAT207.032704E-445.025
GTTCGCC253.8903232E-545.037
TGTTCGC253.8903232E-545.036
CGGTCAA207.032704E-445.06
ATACACG253.8903232E-545.010
CGCTTAT207.032704E-445.023
TAATCGT207.032704E-445.021
CTCGTAG1150.043.0434761
TCGTTAG1400.041.7857131
CGTTAGG2800.041.7857132
GTTAGGG14250.041.368423
TTTTAGG19650.041.3358762
CGTAGGG4900.041.326533
TTAGGGA17800.041.0814634
TTTAGGG33850.041.078293
TTTAGCG556.002665E-1140.9090921
TCGAGGG4400.040.9090923
TAGGGAT9700.040.8247385
CTAGGGA10750.040.8139534
CGTTCAT1050.040.71428717
Produced by FastQC (version 0.11.5)