##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549677_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	959633
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.394323663317124	33.0	31.0	34.0	31.0	34.0
2	32.5644512016573	34.0	31.0	34.0	31.0	34.0
3	32.629172819192334	34.0	31.0	34.0	31.0	34.0
4	36.06969539396832	37.0	35.0	37.0	35.0	37.0
5	35.19630317006606	37.0	35.0	37.0	35.0	37.0
6	26.1720063816063	37.0	0.0	37.0	0.0	37.0
7	31.146810291017502	37.0	19.0	37.0	17.0	37.0
8	34.76598449615634	37.0	32.0	37.0	32.0	37.0
9	37.29277963554817	39.0	37.0	39.0	35.0	39.0
10	37.41046420871312	39.0	37.0	39.0	35.0	39.0
11	37.43681282323555	39.0	37.0	39.0	35.0	39.0
12	37.197667233202694	39.0	37.0	39.0	34.0	39.0
13	37.22294773105968	39.0	37.0	39.0	34.0	39.0
14	38.425584572435504	40.0	38.0	41.0	34.0	41.0
15	38.51923599959568	40.0	38.0	41.0	34.0	41.0
16	38.59644676662849	40.0	38.0	41.0	35.0	41.0
17	38.55328964301978	40.0	38.0	41.0	34.0	41.0
18	38.53991578030351	40.0	38.0	41.0	34.0	41.0
19	38.55303746328023	40.0	38.0	41.0	34.0	41.0
20	38.499617041098006	40.0	38.0	41.0	34.0	41.0
21	38.41481899851297	40.0	37.0	41.0	34.0	41.0
22	38.38842557519385	40.0	37.0	41.0	34.0	41.0
23	38.33741544944786	40.0	37.0	41.0	34.0	41.0
24	38.29099562020064	40.0	37.0	41.0	34.0	41.0
25	38.18447573186833	40.0	37.0	41.0	34.0	41.0
26	38.120687804608636	40.0	37.0	41.0	34.0	41.0
27	38.068930518229365	40.0	37.0	41.0	34.0	41.0
28	38.008374034657	40.0	36.0	41.0	34.0	41.0
29	37.97697557295341	40.0	36.0	41.0	34.0	41.0
30	37.752141704172324	40.0	36.0	41.0	33.0	41.0
31	37.773108052766005	40.0	36.0	41.0	33.0	41.0
32	37.650802963216144	40.0	36.0	41.0	33.0	41.0
33	37.58255395552258	40.0	36.0	41.0	33.0	41.0
34	37.50419900107645	40.0	36.0	41.0	33.0	41.0
35	37.45115997469866	40.0	36.0	41.0	33.0	41.0
36	37.400967870008635	40.0	35.0	41.0	33.0	41.0
37	37.34208390082458	40.0	35.0	41.0	33.0	41.0
38	37.281139769057546	40.0	35.0	41.0	33.0	41.0
39	37.26942904214424	40.0	35.0	41.0	33.0	41.0
40	37.15670157237194	39.0	35.0	41.0	32.0	41.0
41	36.964742771455334	39.0	35.0	41.0	32.0	41.0
42	36.886205455627305	39.0	35.0	41.0	31.0	41.0
43	36.85688070335222	39.0	35.0	41.0	32.0	41.0
44	36.78340886568094	39.0	35.0	41.0	31.0	41.0
45	36.78982381806378	39.0	35.0	41.0	31.0	41.0
46	36.67039274389272	39.0	35.0	41.0	31.0	41.0
47	36.56941455744019	39.0	35.0	41.0	31.0	41.0
48	36.52168068417822	39.0	35.0	40.0	31.0	41.0
49	36.47423963119234	39.0	35.0	40.0	31.0	41.0
50	36.37282481948829	38.0	35.0	40.0	31.0	41.0
51	35.29343613652303	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	8.0
10	11.0
11	14.0
12	6.0
13	11.0
14	9.0
15	21.0
16	38.0
17	72.0
18	148.0
19	299.0
20	479.0
21	827.0
22	1300.0
23	1901.0
24	2736.0
25	4222.0
26	5795.0
27	6960.0
28	8381.0
29	9842.0
30	12630.0
31	17288.0
32	23975.0
33	39808.0
34	61049.0
35	79571.0
36	90023.0
37	150778.0
38	216328.0
39	224964.0
40	133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.015847725119915	4.099067039170183	53.49222046344801	18.392864772261895
2	19.552474748158932	5.312760190614537	53.7924394013128	21.342325659913737
3	21.6163887652884	5.099032651023881	51.35984277322685	21.924735810460874
4	19.683670736625356	4.748169352241951	51.4431037698787	24.125056141254
5	19.101156379574274	8.10278512723093	48.061290097360136	24.734768395834656
6	16.884684040669715	31.43086992631558	38.14687489904995	13.537571133964754
7	83.7179421716427	2.295565075398616	9.465597785820204	4.52089496713848
8	84.73145462900922	3.1730880451172476	7.371463882546765	4.723993443326772
9	79.31813516208801	4.740249657942151	9.64410352707754	6.297511652892304
10	42.983828192652815	24.75686017467094	17.128110433884622	15.131201198791622
11	31.333749464639087	25.97044911961135	23.980209100770818	18.715592314978746
12	31.374702620689366	21.314085697344716	27.66766044935929	19.643551232606633
13	26.777215873151505	21.67630750505662	29.112796246064903	22.43368037572697
14	20.516905942167472	25.212138390405496	29.599857445502604	24.671098221924424
15	18.486754832316105	26.253578190829202	34.39450289850391	20.865164078350787
16	22.12595856957816	24.776659410420443	32.05433743941694	21.043044580584453
17	20.95988779043655	24.210505474488684	29.973646175152375	24.855960559922387
18	21.21613158363666	25.793193856401352	29.674886128342813	23.315788431619172
19	24.2755303329502	26.721673806548964	28.226832549526748	20.77596331097409
20	28.35083828922098	25.399501684498137	27.82751322641051	18.42214679987037
21	26.857038055173177	25.57602750218052	27.522083963348486	20.044850479297814
22	22.98930945476031	25.04874259222015	28.313011328289043	23.648936624730496
23	24.177576219242148	25.543619279453704	27.801461600424325	22.477342900879815
24	24.912023659044653	22.949398363749477	28.026860268456794	24.111717708749072
25	23.341631644597467	25.17681238556823	25.795694812496027	25.685861157338273
26	22.134816122413465	27.409540939088174	26.991151825750055	23.464491112748313
27	22.1400264476107	27.925467340118566	28.00737365221913	21.927132560051604
28	20.1056028710976	27.70090232411766	28.922723582869704	23.270771221915044
29	22.611248258448803	26.616008411548997	27.868674795468685	22.904068534533515
30	24.408602038487633	25.233604930218114	27.60742909007923	22.750363941215028
31	23.389983462427825	26.93217094451733	25.55049690871406	24.127348684340785
32	24.957145075252726	28.474427202899445	25.18900454652977	21.379423175318063
33	25.055203395464726	27.690064847707408	25.411798051963615	21.842933704864258
34	23.60152266543564	26.102999792629056	27.260629844951147	23.03484769698416
35	23.135719592802666	25.68648639636194	26.605483554650576	24.572310456184812
36	22.75130179975053	29.503049603337946	25.37365847151984	22.371990125391687
37	22.090111532221172	29.721466435606114	26.846096372258977	21.342325659913737
38	22.28320618403077	27.5346929503258	25.84415083683033	24.3379500288131
39	24.784683311224185	26.04568621545945	26.667903250513476	22.501727222802884
40	26.0933085877622	23.260663191032403	28.885209241449594	21.760818979755804
41	21.68579029691559	26.80628948775209	28.141174803284173	23.366745412048147
42	22.166078073596886	26.805768455232364	27.097338253269736	23.93081521790101
43	22.607496824306793	26.845158513723476	26.397278959768993	24.150065702200738
44	22.695342907132204	25.26205330579503	26.57047016932515	25.472133617747616
45	24.27625977847781	23.915392655317188	25.32614030572104	26.482207260483953
46	22.81174157203848	26.26076843960139	26.76825411381226	24.159235874547875
47	20.828796008474075	25.95408869849203	29.369144245768958	23.84797104726494
48	21.503533121516245	24.72914124462164	29.012132763254282	24.755192870607825
49	22.489743474849238	24.19810490051926	29.456886122090424	23.855265502541076
50	22.055202353399686	23.87339743422746	27.605865992520055	26.4655342198528
51	20.153121036896398	23.824003551357652	27.38953329033078	28.63334212141517
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	184.0
1	664.5
2	1145.0
3	2918.0
4	4691.0
5	3245.5
6	1800.0
7	1755.0
8	1710.0
9	1740.0
10	1770.0
11	1796.0
12	1822.0
13	1759.0
14	1696.0
15	1712.0
16	1728.0
17	1639.5
18	1551.0
19	1913.5
20	2276.0
21	2325.5
22	2375.0
23	2876.5
24	3378.0
25	4292.0
26	6335.0
27	7464.0
28	9039.0
29	10614.0
30	13050.5
31	15487.0
32	17647.0
33	19807.0
34	22502.0
35	25197.0
36	27387.5
37	29578.0
38	31421.5
39	33265.0
40	38147.0
41	43029.0
42	47460.0
43	51891.0
44	56485.5
45	61080.0
46	72039.0
47	82998.0
48	90021.0
49	97044.0
50	95834.0
51	94624.0
52	83418.0
53	72212.0
54	65358.0
55	58504.0
56	54864.0
57	51224.0
58	49752.5
59	48281.0
60	46052.0
61	43823.0
62	37103.5
63	30384.0
64	24624.5
65	18865.0
66	15128.5
67	11392.0
68	9611.5
69	7831.0
70	6694.0
71	5557.0
72	4773.0
73	3989.0
74	2986.5
75	1418.5
76	853.0
77	704.5
78	556.0
79	506.0
80	456.0
81	301.0
82	146.0
83	112.0
84	78.0
85	58.5
86	39.0
87	37.5
88	36.0
89	21.5
90	7.0
91	6.0
92	5.0
93	2.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	959633.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.196821308834124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.84041191276518	22.486392624906703
2	11.399455329126337	7.340524524998352
3	4.174671251707148	4.032334329430257
4	2.319069261931967	2.986666345169335
5	1.498985180128677	2.413127899459677
6	1.1562743694755493	2.2337015554793465
7	0.912694495710914	2.0570103111572537
8	0.7939794954981341	2.0450892751545346
9	0.6926607816078715	2.0071327871759412
>10	7.030068938469218	44.73407452061849
>50	0.1344250315332606	2.8698858910887206
>100	0.04185889281752887	2.3638847403729044
>500	0.0023822134123759934	0.5061084460819655
>1k	0.0030628458159119914	1.9240667489065408
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	3501	0.36482697031052497	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGCT	2996	0.31220268581843263	TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGC	2040	0.2125812680472639	No Hit
GCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	1803	0.18788432661236118	TruSeq Adapter, Index 15 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	1713	0.17850574125733484	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1698	0.17694264369816376	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTC	1392	0.14505545349107418	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCG	1281	0.13348853155320836	No Hit
CCTGTATCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	1136	0.11837858848122147	TruSeq Adapter, Index 21 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.084130078894744E-4	0.0	0.0	0.33512811668627485	0.0
2	2.084130078894744E-4	0.0	0.0	1.1375181970607513	0.0
3	2.084130078894744E-4	0.0	0.0	1.5356912486335923	0.0
4	2.084130078894744E-4	0.0	0.0	2.0074340919914175	0.0
5	2.084130078894744E-4	0.0	0.0	2.8675545755512784	0.0
6	2.084130078894744E-4	0.0	0.0	3.3210612807187747	0.0
7	2.084130078894744E-4	0.0	0.0	3.972872962893106	0.0
8	2.084130078894744E-4	0.0	0.0	4.956061327611701	0.0
9	2.084130078894744E-4	0.0	0.0	5.38101545069834	0.0
10	2.084130078894744E-4	0.0	0.0	6.387337659292667	0.0
11	2.084130078894744E-4	0.0	0.0	8.093614954883794	0.0
12	2.084130078894744E-4	0.0	0.0	9.842512710588318	0.0
13	2.084130078894744E-4	0.0	0.0	10.34343337505067	0.0
14	2.084130078894744E-4	0.0	0.0	10.538403743931275	0.0
15	2.084130078894744E-4	0.0	0.0	10.856025167954833	0.0
16	2.084130078894744E-4	0.0	0.0	11.494185798112403	0.0
17	2.084130078894744E-4	0.0	0.0	12.29657587848688	0.0
18	2.084130078894744E-4	0.0	0.0	13.142836897022091	0.0
19	2.084130078894744E-4	0.0	0.0	13.85904819863427	0.0
20	2.084130078894744E-4	0.0	0.0	14.637783402613291	0.0
21	2.084130078894744E-4	0.0	0.0	15.702982285936395	0.0
22	2.084130078894744E-4	0.0	0.0	16.838312146414307	0.0
23	2.084130078894744E-4	0.0	0.0	17.880481392365624	0.0
24	2.084130078894744E-4	0.0	0.0	18.665260573573438	0.0
25	2.084130078894744E-4	0.0	0.0	19.358025411798053	0.0
26	2.084130078894744E-4	0.0	0.0	19.974198469623282	0.0
27	2.084130078894744E-4	0.0	0.0	20.572760628281852	0.0
28	2.084130078894744E-4	0.0	0.0	21.19278932675304	0.0
29	2.084130078894744E-4	0.0	0.0	21.857731028424407	0.0
30	2.084130078894744E-4	0.0	0.0	22.5786316227141	0.0
31	2.084130078894744E-4	0.0	0.0	23.30151214057874	0.0
32	2.084130078894744E-4	0.0	0.0	23.996986347905917	0.0
33	2.084130078894744E-4	0.0	0.0	24.64640128048952	0.0
34	2.084130078894744E-4	0.0	0.0	25.305090591924206	0.0
35	2.084130078894744E-4	0.0	0.0	25.95033726435002	0.0
36	2.084130078894744E-4	0.0	0.0	26.665610707426694	0.0
37	2.084130078894744E-4	0.0	0.0	27.36108491475387	0.0
38	2.084130078894744E-4	0.0	0.0	28.022066769275337	0.0
39	2.084130078894744E-4	0.0	0.0	28.754325872495006	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGAT	20	7.032704E-4	45.0	25
GTTCGCC	25	3.8903232E-5	45.0	37
TGTTCGC	25	3.8903232E-5	45.0	36
CGGTCAA	20	7.032704E-4	45.0	6
ATACACG	25	3.8903232E-5	45.0	10
CGCTTAT	20	7.032704E-4	45.0	23
TAATCGT	20	7.032704E-4	45.0	21
CTCGTAG	115	0.0	43.043476	1
TCGTTAG	140	0.0	41.785713	1
CGTTAGG	280	0.0	41.785713	2
GTTAGGG	1425	0.0	41.36842	3
TTTTAGG	1965	0.0	41.335876	2
CGTAGGG	490	0.0	41.32653	3
TTAGGGA	1780	0.0	41.081463	4
TTTAGGG	3385	0.0	41.07829	3
TTTAGCG	55	6.002665E-11	40.909092	1
TCGAGGG	440	0.0	40.909092	3
TAGGGAT	970	0.0	40.824738	5
CTAGGGA	1075	0.0	40.813953	4
CGTTCAT	105	0.0	40.714287	17
>>END_MODULE