SRR3549674_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549674_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1141252
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11055	0.9686730012302278	No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	10630	0.9314331979264878	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	10546	0.9240728603323367	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCC	9063	0.794127852568933	No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	8073	0.7073810166378679	No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	7141	0.6257163185694308	No Hit
TCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG	3448	0.30212433362657853	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGC	3338	0.2924857963009046	No Hit
TTCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT	2903	0.2543697623311942	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTC	2693	0.23596891834581668	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCG	2337	0.20477510663727208	No Hit
TTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG	2281	0.19986821490783807	No Hit
TGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG	2160	0.18926582384959675	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC	1855	0.16254078853750092	No Hit
CGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG	1810	0.15859775054063432	No Hit
TTTCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC	1794	0.15719578147508176	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCT	1602	0.14037215268845094	No Hit
GCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG	1597	0.13993403735546575	No Hit
TTGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT	1534	0.1344137841598525	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGT	1503	0.1316974690953444	No Hit
TTTTTTGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTC	1315	0.11522433257510174	No Hit
GTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG	1302	0.11408523270934026	No Hit
GTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC	1271	0.11136891764483216	No Hit
GTTCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC	1200	0.10514767991644264	No Hit
TTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT	1192	0.10444669538366635	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	125	0.0	45.000004	24
ACAGTTC	25	3.8907885E-5	45.0	12
CTCGTAG	25	3.8907885E-5	45.0	1
CGCGGCC	25	3.8907885E-5	45.0	35
GACAACG	25	3.8907885E-5	45.0	11
GTGATCG	20	7.033266E-4	45.0	23
GTTAGCG	25	3.8907885E-5	45.0	1
TTACTCG	35	1.2119199E-7	45.0	1
AACGGTA	20	7.033266E-4	45.0	25
TACGCGG	70	0.0	45.0	2
TCGGAAA	20	7.033266E-4	45.0	14
TGACCGG	25	3.8907885E-5	45.0	2
GCACAAC	20	7.033266E-4	45.0	12
TACGACT	20	7.033266E-4	45.0	14
CCGAGCC	35	1.2119199E-7	45.0	43
GTCCGCG	45	3.8562575E-10	45.0	1
TCTCCCG	55	1.8189894E-12	45.0	1
TTACGCG	20	7.033266E-4	45.0	1
CGCGTAT	35	1.2119199E-7	45.0	24
TCGACGG	25	3.8907885E-5	45.0	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content