##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549674_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1141252
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2024364469898	33.0	31.0	34.0	30.0	34.0
2	32.36934261670516	34.0	31.0	34.0	30.0	34.0
3	32.38818858586885	34.0	31.0	34.0	30.0	34.0
4	35.90318790240893	37.0	35.0	37.0	35.0	37.0
5	35.94073000529243	37.0	35.0	37.0	35.0	37.0
6	35.784389424947335	37.0	35.0	37.0	35.0	37.0
7	35.93062531325246	37.0	35.0	37.0	35.0	37.0
8	35.903318460778166	37.0	35.0	37.0	35.0	37.0
9	37.78342119006144	39.0	38.0	39.0	35.0	39.0
10	37.310024429310964	39.0	37.0	39.0	34.0	39.0
11	37.13240633970411	39.0	37.0	39.0	34.0	39.0
12	37.12131764062626	39.0	37.0	39.0	34.0	39.0
13	37.08594858979436	39.0	37.0	39.0	33.0	39.0
14	38.16324615422361	40.0	38.0	41.0	33.0	41.0
15	38.238098158864126	40.0	38.0	41.0	33.0	41.0
16	38.322431855541105	40.0	38.0	41.0	34.0	41.0
17	38.347629620802415	40.0	38.0	41.0	34.0	41.0
18	38.38716427222033	40.0	38.0	41.0	34.0	41.0
19	38.30644940819381	40.0	37.0	41.0	34.0	41.0
20	38.21269535562698	40.0	37.0	41.0	34.0	41.0
21	38.14739338901487	40.0	37.0	41.0	34.0	41.0
22	38.04613354456334	40.0	37.0	41.0	34.0	41.0
23	37.78476795659504	39.0	36.0	41.0	33.0	41.0
24	37.394703360870345	39.0	36.0	41.0	32.0	41.0
25	37.34894046187871	39.0	36.0	41.0	32.0	41.0
26	37.51691475677589	39.0	36.0	41.0	33.0	41.0
27	37.64050095859635	39.0	36.0	41.0	33.0	41.0
28	37.67226432023777	39.0	36.0	41.0	33.0	41.0
29	37.66563300655771	39.0	36.0	41.0	33.0	41.0
30	37.51561530669826	39.0	36.0	41.0	33.0	41.0
31	37.39580040166414	39.0	35.0	41.0	33.0	41.0
32	37.23400090427005	39.0	35.0	41.0	33.0	41.0
33	36.95438080283758	39.0	35.0	41.0	32.0	41.0
34	36.897618580296026	39.0	35.0	41.0	32.0	41.0
35	36.86742542400802	39.0	35.0	41.0	31.0	41.0
36	36.69974379015327	39.0	35.0	40.0	31.0	41.0
37	36.69034183510741	39.0	35.0	40.0	31.0	41.0
38	36.60886903155482	39.0	35.0	40.0	31.0	41.0
39	36.45412319102179	39.0	35.0	40.0	31.0	41.0
40	36.42749629354428	38.0	35.0	40.0	31.0	41.0
41	36.390697234265524	39.0	35.0	40.0	31.0	41.0
42	36.243797163115595	38.0	35.0	40.0	30.0	41.0
43	36.1190867573507	38.0	35.0	40.0	30.0	41.0
44	36.11461798095425	38.0	35.0	40.0	30.0	41.0
45	36.157810019171926	38.0	35.0	40.0	30.0	41.0
46	36.00710097331702	38.0	35.0	40.0	30.0	41.0
47	35.928570552340766	38.0	35.0	40.0	30.0	41.0
48	35.872051921924346	38.0	35.0	40.0	30.0	41.0
49	35.83772821427695	38.0	35.0	40.0	29.0	41.0
50	35.70490216008384	38.0	35.0	40.0	28.0	41.0
51	35.01983260489357	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	3.0
11	4.0
12	3.0
13	9.0
14	12.0
15	15.0
16	35.0
17	70.0
18	155.0
19	286.0
20	572.0
21	1058.0
22	1618.0
23	2673.0
24	4084.0
25	6217.0
26	8547.0
27	10199.0
28	11551.0
29	14079.0
30	17746.0
31	22884.0
32	30028.0
33	40542.0
34	63906.0
35	87188.0
36	127519.0
37	165770.0
38	257548.0
39	266781.0
40	147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.38226439033623	4.263738420611749	60.18328993070769	15.170707258344345
2	20.689733731025225	3.7840021309929797	55.0630360341099	20.463228103871888
3	17.59839194148181	3.634166687112049	57.55126825626593	21.216173115140215
4	16.497583355823252	3.751844465551868	56.916526761837005	22.83404541678788
5	15.534605853921835	4.371514792526103	57.343426342297754	22.750453011254308
6	19.357775495683686	5.22198427691693	58.12984336500615	17.29039686239323
7	78.2157665441112	1.1102718768510373	14.622712599846485	6.051248979191274
8	79.03688230119202	1.533491288514719	12.04896026469176	7.380666145601497
9	74.31794204960867	4.002358812952792	15.251057610413826	6.428641527024706
10	36.96554310529138	30.87249792333332	19.70230939354323	12.459649577832064
11	20.339153841570486	26.54383081037317	32.00160875950272	21.115406588553622
12	18.636900526789876	23.952028123499456	36.38442692761984	21.026644422090826
13	20.713129089806635	25.792813506569978	36.04453705228995	17.449520351333447
14	17.425073515752874	28.053313378640297	34.0891406981105	20.43247240749633
15	13.274018358784915	29.400430404503126	35.39814169000361	21.92740954670835
16	14.562077437761337	31.27021902261727	31.47876192111821	22.68894161850319
17	14.801025540371452	32.842790198834265	29.017079488141096	23.33910477265319
18	15.958613873184888	29.539751080392413	33.884453214539825	20.61718183188288
19	15.224683067368119	32.113678661680325	31.920557422900465	20.741080848051087
20	18.261348063354983	35.18767108403753	29.316575129769763	17.234405722837725
21	17.46248856518981	36.54959640815525	28.685426181071318	17.30248884558362
22	16.99011261316519	34.43753001090031	26.192374690252464	22.37998268568204
23	15.850749878203937	34.42745335824165	25.37003221023928	24.35176455331513
24	17.87834763925934	33.63796952820236	26.649679474822385	21.83400335771591
25	14.784990519184193	35.351613841640585	23.615993663099825	26.247401976075395
26	14.664333556480077	35.18793395323732	26.17914360719631	23.968588883086294
27	15.294518651445957	33.47560398579805	29.60993715673664	21.619940206019354
28	13.446635799981074	31.0917308359591	28.206215629852128	27.255417734207693
29	13.6705127351365	29.559378647310147	28.35123180507022	28.418876812483134
30	19.267173244822352	33.15832086165019	26.0198448721229	21.554661021404563
31	19.218104327528014	35.74214984946357	21.410433453785842	23.629312369222575
32	18.156375629571734	34.189469109364104	21.18497930343167	26.469175957632494
33	17.81771247717419	33.67485883923971	24.663439801200788	23.84398888238531
34	17.40640980256771	30.24003462863592	24.209201823961752	28.14435374483462
35	16.880320910719103	29.479028295240667	24.00626680172302	29.634383992317208
36	17.357779000606353	31.182771202153425	27.96148440484661	23.497965392393617
37	16.305163101576163	31.206692299334414	28.79907329844767	23.68907130064175
38	16.58231486122259	33.09216544636943	25.14536666748448	25.180153024923506
39	18.226123590582972	34.602436622235935	26.396974550756536	20.774465236424557
40	23.901206744873175	31.889363611191918	25.052135724625234	19.15729391930967
41	22.71785723048021	29.798764865253247	24.54874120702527	22.934636697241277
42	20.253020367105602	28.454101285255142	28.354123366267924	22.938754981371336
43	18.12150164906611	28.266850791937276	30.864962339605977	22.746685219390635
44	16.779554384132513	29.014100303876795	25.706855278238283	28.499490033752405
45	19.508049054897604	29.3894775211785	23.5990824112466	27.5033910126773
46	20.818013900523287	29.93203954954734	27.48980943735477	21.76013711257461
47	16.112479978129283	31.262245323556936	29.301153470048686	23.3241212282651
48	16.056751707773568	28.578175547556544	30.70785418119749	24.657218563472398
49	19.78747901427555	27.33191267134691	30.194908749338445	22.685699565039098
50	19.641236116125096	25.6473592160189	27.225450645431508	27.485954022424497
51	17.436464514410492	25.68968115718527	25.090865120061125	31.782989208343114
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	633.0
1	1148.0
2	1663.0
3	7363.5
4	13064.0
5	7868.5
6	2673.0
7	3724.0
8	4775.0
9	7677.0
10	10579.0
11	13579.5
12	16580.0
13	17787.0
14	18994.0
15	18012.0
16	17030.0
17	15981.0
18	14932.0
19	12839.0
20	10746.0
21	9444.0
22	8142.0
23	7884.5
24	7627.0
25	7896.5
26	10295.0
27	12424.0
28	15387.5
29	18351.0
30	20439.5
31	22528.0
32	25888.0
33	29248.0
34	31253.0
35	33258.0
36	33499.0
37	33740.0
38	35861.0
39	37982.0
40	39741.0
41	41500.0
42	46766.0
43	52032.0
44	62142.0
45	72252.0
46	81624.5
47	90997.0
48	112215.0
49	133433.0
50	149905.0
51	166377.0
52	145300.5
53	124224.0
54	93245.5
55	62267.0
56	47199.5
57	32132.0
58	24175.0
59	16218.0
60	13241.0
61	10264.0
62	8089.5
63	5915.0
64	5042.0
65	4169.0
66	3297.0
67	2425.0
68	1816.0
69	1207.0
70	1037.5
71	868.0
72	751.0
73	634.0
74	634.5
75	454.5
76	274.0
77	195.5
78	117.0
79	112.5
80	108.0
81	77.5
82	47.0
83	29.0
84	11.0
85	8.0
86	5.0
87	4.5
88	4.0
89	2.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1141252.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.55871185782737
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.04464334193972	22.805633597545018
2	12.572739005939507	8.187043731161028
3	5.302895214556239	5.179663119089647
4	2.8904976551272092	3.7644352111604995
5	1.8292809335169895	2.9779515410698565
6	1.2164348077886895	2.376333024037414
7	0.8669718849542867	1.9759241453744858
8	0.6344491210786737	1.6525476897321894
9	0.500270708221961	1.465935285591905
>10	3.6854169570187154	24.23260283586439
>50	0.31858480461003896	6.928454633350205
>100	0.12014690304438147	7.542129051436734
>500	0.010057546177444584	2.3365651912531806
>1k	0.005980162591994077	3.573658795409726
>5k	8.154767170901013E-4	2.1485850213269746
>10k+	8.154767170901013E-4	2.8525371265967676
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11055	0.9686730012302278	No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	10630	0.9314331979264878	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	10546	0.9240728603323367	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCC	9063	0.794127852568933	No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	8073	0.7073810166378679	No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	7141	0.6257163185694308	No Hit
TCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG	3448	0.30212433362657853	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGC	3338	0.2924857963009046	No Hit
TTCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT	2903	0.2543697623311942	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTC	2693	0.23596891834581668	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCG	2337	0.20477510663727208	No Hit
TTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG	2281	0.19986821490783807	No Hit
TGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG	2160	0.18926582384959675	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC	1855	0.16254078853750092	No Hit
CGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG	1810	0.15859775054063432	No Hit
TTTCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC	1794	0.15719578147508176	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCT	1602	0.14037215268845094	No Hit
GCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG	1597	0.13993403735546575	No Hit
TTGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT	1534	0.1344137841598525	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGT	1503	0.1316974690953444	No Hit
TTTTTTGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTC	1315	0.11522433257510174	No Hit
GTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG	1302	0.11408523270934026	No Hit
GTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC	1271	0.11136891764483216	No Hit
GTTCCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC	1200	0.10514767991644264	No Hit
TTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT	1192	0.10444669538366635	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9903158986796956	0.0
2	0.0	0.0	0.0	3.4623378535152622	0.0
3	0.0	0.0	0.0	4.856771335340485	0.0
4	0.0	0.0	0.0	6.481653482315913	0.0
5	0.0	0.0	0.0	8.97137529660408	0.0
6	0.0	0.0	0.0	10.592840143982222	0.0
7	0.0	0.0	0.0	12.237437480941983	0.0
8	0.0	0.0	0.0	14.85219741126412	0.0
9	0.0	0.0	0.0	16.14174608237269	0.0
10	0.0	0.0	0.0	18.349321622218405	0.0
11	0.0	0.0	0.0	23.42392390111912	0.0
12	0.0	0.0	0.0	26.865056972517902	0.0
13	0.0	0.0	0.0	27.893839397433695	0.0
14	0.0	0.0	0.0	28.18693855520078	0.0
15	0.0	0.0	0.0	28.561001426503523	0.0
16	0.0	0.0	0.0	29.483409448570516	0.0
17	8.762306659703554E-5	0.0	0.0	31.083406644632387	0.0
18	8.762306659703554E-5	0.0	0.0	32.79039160500923	0.0
19	2.628691997911066E-4	0.0	0.0	34.01396010697024	0.0
20	2.628691997911066E-4	0.0	0.0	35.05790132240732	0.0
21	2.628691997911066E-4	0.0	0.0	36.50578487485674	0.0
22	2.628691997911066E-4	0.0	0.0	38.02946237991259	0.0
23	2.628691997911066E-4	0.0	0.0	39.487597831153856	0.0
24	2.628691997911066E-4	0.0	0.0	40.59918405400385	0.0
25	2.628691997911066E-4	0.0	0.0	41.45236985345918	0.0
26	2.628691997911066E-4	0.0	0.0	42.17753835261625	0.0
27	2.628691997911066E-4	0.0	0.0	42.90813948190233	0.0
28	2.628691997911066E-4	0.0	0.0	43.625947643465246	0.0
29	2.628691997911066E-4	0.0	0.0	44.38371192339641	0.0
30	2.628691997911066E-4	0.0	0.0	45.21034793367284	0.0
31	3.5049226638814217E-4	0.0	0.0	45.968462705870394	0.0
32	3.5049226638814217E-4	0.0	0.0	46.63194456614315	0.0
33	3.5049226638814217E-4	0.0	0.0	47.29498831108292	0.0
34	3.5049226638814217E-4	0.0	0.0	47.917024460855274	0.0
35	3.5049226638814217E-4	0.0	0.0	48.62405498522675	0.0
36	3.5049226638814217E-4	0.0	0.0	49.28262995377007	0.0
37	3.5049226638814217E-4	0.0	0.0	49.908171026206304	0.0
38	3.5049226638814217E-4	0.0	0.0	50.50760042479663	0.0
39	4.3811533298517767E-4	0.0	0.0	51.09388636339739	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	125	0.0	45.000004	24
ACAGTTC	25	3.8907885E-5	45.0	12
CTCGTAG	25	3.8907885E-5	45.0	1
CGCGGCC	25	3.8907885E-5	45.0	35
GACAACG	25	3.8907885E-5	45.0	11
GTGATCG	20	7.033266E-4	45.0	23
GTTAGCG	25	3.8907885E-5	45.0	1
TTACTCG	35	1.2119199E-7	45.0	1
AACGGTA	20	7.033266E-4	45.0	25
TACGCGG	70	0.0	45.0	2
TCGGAAA	20	7.033266E-4	45.0	14
TGACCGG	25	3.8907885E-5	45.0	2
GCACAAC	20	7.033266E-4	45.0	12
TACGACT	20	7.033266E-4	45.0	14
CCGAGCC	35	1.2119199E-7	45.0	43
GTCCGCG	45	3.8562575E-10	45.0	1
TCTCCCG	55	1.8189894E-12	45.0	1
TTACGCG	20	7.033266E-4	45.0	1
CGCGTAT	35	1.2119199E-7	45.0	24
TCGACGG	25	3.8907885E-5	45.0	2
>>END_MODULE