FastQCFastQC Report
Sat 18 Jun 2016
SRR3549673_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549673_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1323700
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75430.5698421092392536No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCC73490.5551862204426985No Hit
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT65700.4963360278008613RNA PCR Primer, Index 34 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC51580.3896653320238725TruSeq Adapter, Index 18 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC41490.3134396011180781RNA PCR Primer, Index 34 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC32210.24333308151393823TruSeq Adapter, Index 18 (95% over 23bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGC27230.20571126388154418No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTC21140.15970386039132733No Hit
TCCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG20300.15335801163405605RNA PCR Primer, Index 34 (95% over 24bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCG18340.13855103120042306No Hit
TTCCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT13530.10221349248319106TruSeq Adapter, Index 18 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAC207.033674E-445.00000434
AACCCGA207.033674E-445.00000424
CGTCTAT207.033674E-445.00000440
TCGTCTA207.033674E-445.00000439
TGTCGTA253.8911265E-545.00000418
ACGGTAG207.033674E-445.00000419
TAATCGT207.033674E-445.00000421
CGGGTCG453.8562575E-1045.06
CGATAGC453.8562575E-1045.010
CGTTTTT43550.042.881751
TTACCGG700.041.7857132
TGTTTCG3100.040.645161
CGGGTAA950.040.2631536
TACGGGA2000.039.3750044
TAGGGCG1500.039.05
TGGGCGA20800.038.725966
TTTCGTG3900.038.6538471
GCGGGTA1400.038.5714265
TAGTGCG356.249009E-638.5714261
GCCCGCG356.249009E-638.5714261
Produced by FastQC (version 0.11.5)