##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549673_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1323700
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.382969706126765	33.0	31.0	34.0	31.0	34.0
2	32.539011105235325	34.0	31.0	34.0	31.0	34.0
3	32.552775553373124	34.0	31.0	34.0	31.0	34.0
4	36.01726373045252	37.0	35.0	37.0	35.0	37.0
5	36.047877917957244	37.0	35.0	37.0	35.0	37.0
6	35.87903981264637	37.0	35.0	37.0	35.0	37.0
7	36.021352270151844	37.0	35.0	37.0	35.0	37.0
8	36.01350154868928	37.0	35.0	37.0	35.0	37.0
9	37.88882979527083	39.0	38.0	39.0	35.0	39.0
10	37.43417164009972	39.0	37.0	39.0	35.0	39.0
11	37.262550426833876	39.0	37.0	39.0	34.0	39.0
12	37.174989045856314	39.0	37.0	39.0	34.0	39.0
13	37.19097303014278	39.0	37.0	39.0	34.0	39.0
14	38.309774118002565	40.0	38.0	41.0	34.0	41.0
15	38.379997733625444	40.0	38.0	41.0	34.0	41.0
16	38.43147314346151	40.0	38.0	41.0	34.0	41.0
17	38.43854876482587	40.0	38.0	41.0	34.0	41.0
18	38.44538339502908	40.0	38.0	41.0	34.0	41.0
19	38.43667220669336	40.0	37.0	41.0	34.0	41.0
20	38.34233965400015	40.0	37.0	41.0	34.0	41.0
21	38.27936239329153	40.0	37.0	41.0	34.0	41.0
22	38.18256931328851	40.0	37.0	41.0	34.0	41.0
23	37.96005137115661	40.0	37.0	41.0	33.0	41.0
24	37.64384679308	39.0	36.0	41.0	33.0	41.0
25	37.595966608748206	39.0	36.0	41.0	33.0	41.0
26	37.7221613658684	39.0	36.0	41.0	33.0	41.0
27	37.79481680139004	40.0	36.0	41.0	33.0	41.0
28	37.78110070257611	40.0	36.0	41.0	33.0	41.0
29	37.76791493540833	40.0	36.0	41.0	33.0	41.0
30	37.64298254891592	40.0	36.0	41.0	33.0	41.0
31	37.51581325073657	40.0	36.0	41.0	33.0	41.0
32	37.38882752889628	39.0	35.0	41.0	33.0	41.0
33	37.24682480924681	39.0	35.0	41.0	32.0	41.0
34	37.11217798594848	39.0	35.0	41.0	32.0	41.0
35	37.047581778348565	39.0	35.0	41.0	31.0	41.0
36	36.92822920601345	39.0	35.0	41.0	31.0	41.0
37	36.86718894009216	39.0	35.0	41.0	31.0	41.0
38	36.81687391402886	39.0	35.0	41.0	31.0	41.0
39	36.784834932386495	39.0	35.0	41.0	31.0	41.0
40	36.675760368663596	39.0	35.0	41.0	31.0	41.0
41	36.62223540077057	39.0	35.0	41.0	31.0	41.0
42	36.63132658457354	39.0	35.0	41.0	31.0	41.0
43	36.528391629523306	39.0	35.0	41.0	31.0	41.0
44	36.42500037772909	39.0	35.0	41.0	31.0	41.0
45	36.35929440205484	39.0	35.0	41.0	31.0	41.0
46	36.19560776611014	39.0	35.0	40.0	30.0	41.0
47	36.07689506685805	38.0	35.0	40.0	30.0	41.0
48	36.01393367077132	38.0	35.0	40.0	30.0	41.0
49	36.011402885850266	38.0	35.0	40.0	30.0	41.0
50	35.86392762710584	38.0	35.0	40.0	29.0	41.0
51	35.078252625217196	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	8.0
11	11.0
12	12.0
13	6.0
14	25.0
15	22.0
16	49.0
17	114.0
18	210.0
19	427.0
20	762.0
21	1254.0
22	2038.0
23	3049.0
24	4683.0
25	7021.0
26	9610.0
27	11654.0
28	13492.0
29	15780.0
30	19461.0
31	24920.0
32	32109.0
33	44453.0
34	74277.0
35	97236.0
36	118559.0
37	170400.0
38	290486.0
39	381315.0
40	245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.453199365415124	3.855631940772078	58.70166956258971	15.989499131223086
2	20.866434992823145	3.8465664425474047	54.68353856614036	20.603459998489086
3	18.089219611694492	3.8387096774193545	56.85094810002267	21.22112261086349
4	17.442471859182596	3.9498375764901414	55.88660572637304	22.72108483795422
5	16.686333761426305	4.886530180554506	54.699327642215	23.727808415804187
6	20.30037017451084	5.931404396766639	56.61192113016544	17.156304298557075
7	83.06753796177382	1.4959582987081665	10.933368588048651	4.503135151469366
8	85.09533882299614	1.8275288962755911	8.624310644405831	4.4528216363224296
9	79.7219913877767	4.8856991765505775	10.785072146256706	4.607237289416031
10	38.53886832363828	32.98285109919166	15.90556772682632	12.572712850343734
11	24.11535846490897	26.049860240235702	30.242426531691468	19.592354763163858
12	22.57150411724711	23.60723728941603	33.76248394651356	20.0587746468233
13	23.067311324318197	22.899599607161743	34.916672962151544	19.11641610636851
14	19.810757724559945	25.69668353856614	33.623253002946285	20.869305733927625
15	17.400090654982247	26.58646218931782	34.98632620684445	21.027120948855483
16	19.688600135982472	27.13507592354763	32.6108634887059	20.565460451763997
17	19.791493540832512	27.021832741557755	31.06814232832213	22.1185313892876
18	19.492256553599756	25.82843544609806	33.24023570295384	21.439072297348343
19	20.01926418372743	27.67439752209715	32.027498677948174	20.27883961622724
20	22.555488403716854	29.230641383999394	30.17511520737327	18.038755004910477
21	22.490367908136285	28.814610561305432	30.487194983757647	18.207826546800636
22	21.016091259348794	27.11203444889325	29.727506232530033	22.144368059227922
23	19.628465664425473	28.869456825564704	28.763012767243335	22.739064742766487
24	20.223615622875275	28.277479791493544	29.469743899675155	22.029160685956032
25	19.204124801692227	29.355140892951574	27.770340711641612	23.670393593714586
26	19.37123215230037	29.828435446098055	28.987459394122535	21.812873007479038
27	18.966910931479944	28.791871269925213	31.2118304751832	21.02938732341165
28	18.578529878371235	28.13900430611166	31.14391478431669	22.138551031200425
29	20.87330966231019	25.9743899675153	30.459394122535315	22.692906247639193
30	22.521115056281634	27.329908589559572	29.48349323864924	20.665483115509556
31	22.10651960413991	30.54861373422981	26.520963964644555	20.823902696985723
32	21.77404245675002	30.463398050917885	25.85555639495354	21.90700309737856
33	21.544911989121402	28.59477222935711	26.68459620759991	23.175719573921583
34	20.669864772984813	28.1745863866435	27.76376822542872	23.391780614942963
35	21.523003701745107	28.114225277630883	27.109012616151695	23.253758404472315
36	21.156379844375614	28.683614111958907	28.348870590012844	21.811135453652643
37	21.482284505552617	29.731661252549674	28.530860466873158	20.25519377502455
38	22.156153206919996	29.964191282012543	26.512880562060893	21.366774949006572
39	21.02976505250434	28.51499584497998	26.73188789000529	23.723351212510387
40	19.978922716627633	30.15668202764977	27.167636171337918	22.69675908438468
41	18.204426984966382	32.07932310946589	26.200498602402355	23.51575130316537
42	20.15449119891214	28.931631034222256	26.44420941300899	24.469668353856612
43	20.843620155624386	26.519906323185012	28.673490972274685	23.962982548915917
44	20.920827982171186	26.32386492407645	27.67658835083478	25.078718742917577
45	22.58366699403188	26.274533504570524	26.501926418372747	24.639873083024856
46	21.924529727279594	27.348341769283067	28.91176248394651	21.81536601949082
47	19.094205635718062	29.373045251945307	29.07660345999849	22.456145652338144
48	19.161743597491878	27.906172093374632	29.893631487497167	23.038452821636323
49	22.029765052504345	25.9511218554053	29.0570370929969	22.96207599909345
50	20.77117171564554	25.599909345017753	27.626954748054693	26.001964191282013
51	19.103422225579813	25.804336329984135	26.609956938883432	28.48228450555262
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	814.0
1	1730.5
2	2647.0
3	6796.0
4	10945.0
5	7141.0
6	3337.0
7	3918.5
8	4500.0
9	5817.0
10	7134.0
11	8506.0
12	9878.0
13	10581.5
14	11285.0
15	11006.5
16	10728.0
17	10323.5
18	9919.0
19	9282.5
20	8646.0
21	8554.5
22	8463.0
23	9095.5
24	9728.0
25	10475.0
26	13253.5
27	15285.0
28	18036.5
29	20788.0
30	23300.5
31	25813.0
32	28334.5
33	30856.0
34	35193.0
35	39530.0
36	42802.0
37	46074.0
38	48781.0
39	51488.0
40	56836.5
41	62185.0
42	67257.5
43	72330.0
44	80734.0
45	89138.0
46	94293.5
47	99449.0
48	113043.5
49	126638.0
50	137809.5
51	148981.0
52	135928.0
53	122875.0
54	102198.0
55	81521.0
56	66752.0
57	51983.0
58	45607.0
59	39231.0
60	34358.5
61	29486.0
62	25437.0
63	21388.0
64	18209.0
65	15030.0
66	12161.5
67	9293.0
68	7360.0
69	5427.0
70	4416.0
71	3405.0
72	2985.0
73	2565.0
74	2137.5
75	1369.0
76	1028.0
77	740.0
78	452.0
79	385.0
80	318.0
81	208.5
82	99.0
83	72.5
84	46.0
85	25.0
86	4.0
87	18.0
88	32.0
89	18.5
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1323700.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.585523107003276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.55867380310804	23.317768187176867
2	11.961526327652523	7.795451850894987
3	4.557189542046986	4.454952153760973
4	2.355025371989409	3.0695893470615947
5	1.453733777692892	2.3685337802221453
6	0.9879463463143849	1.931564909778411
7	0.7521262300206997	1.7155898653405937
8	0.5742605918713412	1.4970065428691972
9	0.45992186034168414	1.3488114966821894
>10	4.8480764378465	34.994476880864326
>50	0.4143410388903969	8.68879099673971
>100	0.06896817535026029	4.058632457143361
>500	0.003987955624962516	0.7986550773552811
>1k	0.003284198749969131	1.9253182842024756
>5k	9.383424999911804E-4	2.034858169907885
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7543	0.5698421092392536	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCC	7349	0.5551862204426985	No Hit
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	6570	0.4963360278008613	RNA PCR Primer, Index 34 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	5158	0.3896653320238725	TruSeq Adapter, Index 18 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	4149	0.3134396011180781	RNA PCR Primer, Index 34 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	3221	0.24333308151393823	TruSeq Adapter, Index 18 (95% over 23bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGC	2723	0.20571126388154418	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTC	2114	0.15970386039132733	No Hit
TCCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG	2030	0.15335801163405605	RNA PCR Primer, Index 34 (95% over 24bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCG	1834	0.13855103120042306	No Hit
TTCCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT	1353	0.10221349248319106	TruSeq Adapter, Index 18 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5109163707788774E-4	0.0	0.0	0.5310115585102364	0.0
2	1.5109163707788774E-4	0.0	0.0	1.571881846339805	0.0
3	1.5109163707788774E-4	0.0	0.0	2.272871496562665	0.0
4	1.5109163707788774E-4	0.0	0.0	3.0197174586386644	0.0
5	1.5109163707788774E-4	0.0	0.0	4.193775024552391	0.0
6	1.5109163707788774E-4	0.0	0.0	5.047140590768301	0.0
7	1.5109163707788774E-4	0.0	0.0	5.880335423434313	0.0
8	1.5109163707788774E-4	0.0	0.0	7.325602477902848	0.0
9	1.5109163707788774E-4	0.0	0.0	8.06141875047216	0.0
10	1.5109163707788774E-4	0.0	0.0	9.331041776837653	0.0
11	1.5109163707788774E-4	0.0	0.0	12.277404245675003	0.0
12	1.5109163707788774E-4	0.0	0.0	14.412177985948478	0.0
13	1.5109163707788774E-4	0.0	0.0	15.061872025383394	0.0
14	1.5109163707788774E-4	0.0	0.0	15.268716476543023	0.0
15	1.5109163707788774E-4	0.0	0.0	15.548236005137115	0.0
16	1.5109163707788774E-4	0.0	0.0	16.20518244315177	0.0
17	1.5109163707788774E-4	0.0	0.0	17.295233058850194	0.0
18	1.5109163707788774E-4	0.0	0.0	18.491274457958752	0.0
19	1.5109163707788774E-4	0.0	0.0	19.403792400090655	0.0
20	1.5109163707788774E-4	0.0	0.0	20.25383395029085	0.0
21	1.5109163707788774E-4	0.0	0.0	21.40575659137267	0.0
22	1.5109163707788774E-4	0.0	0.0	22.598927249376747	0.0
23	1.5109163707788774E-4	0.0	0.0	23.718365188486818	0.0
24	2.266374556168316E-4	0.0	0.0	24.614489687995768	0.0
25	2.266374556168316E-4	0.0	0.0	25.352496789302712	0.0
26	2.266374556168316E-4	0.0	0.0	26.008158948402205	0.0
27	2.266374556168316E-4	0.0	0.0	26.664652111505628	0.0
28	2.266374556168316E-4	0.0	0.0	27.357256175870667	0.0
29	2.266374556168316E-4	0.0	0.0	28.103120042305658	0.0
30	2.266374556168316E-4	0.0	0.0	28.888569917655058	0.0
31	2.266374556168316E-4	0.0	0.0	29.61010803052051	0.0
32	2.266374556168316E-4	0.0	0.0	30.29115358464909	0.0
33	2.266374556168316E-4	0.0	0.0	30.948704389212057	0.0
34	2.266374556168316E-4	0.0	0.0	31.607010651960415	0.0
35	2.266374556168316E-4	0.0	0.0	32.297348341769286	0.0
36	2.266374556168316E-4	0.0	0.0	32.96887512276196	0.0
37	2.266374556168316E-4	0.0	0.0	33.63307395935635	0.0
38	2.266374556168316E-4	0.0	0.0	34.29236231774571	0.0
39	2.266374556168316E-4	0.0	0.0	34.98277555337312	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTAC	20	7.033674E-4	45.000004	34
AACCCGA	20	7.033674E-4	45.000004	24
CGTCTAT	20	7.033674E-4	45.000004	40
TCGTCTA	20	7.033674E-4	45.000004	39
TGTCGTA	25	3.8911265E-5	45.000004	18
ACGGTAG	20	7.033674E-4	45.000004	19
TAATCGT	20	7.033674E-4	45.000004	21
CGGGTCG	45	3.8562575E-10	45.0	6
CGATAGC	45	3.8562575E-10	45.0	10
CGTTTTT	4355	0.0	42.88175	1
TTACCGG	70	0.0	41.785713	2
TGTTTCG	310	0.0	40.64516	1
CGGGTAA	95	0.0	40.263153	6
TACGGGA	200	0.0	39.375004	4
TAGGGCG	150	0.0	39.0	5
TGGGCGA	2080	0.0	38.72596	6
TTTCGTG	390	0.0	38.653847	1
GCGGGTA	140	0.0	38.571426	5
TAGTGCG	35	6.249009E-6	38.571426	1
GCCCGCG	35	6.249009E-6	38.571426	1
>>END_MODULE