FastQCFastQC Report
Sat 18 Jun 2016
SRR3549672_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549672_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896980
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCG128190.6757583105778658No Hit
TCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC127440.6718046579299729No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC116110.6120781452624698No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT114630.6042762707039611No Hit
GAATCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTC105380.5555145547132812No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCC102350.5395417980157935No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGC101860.5369587449525035No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88640.4672690276123101No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC76420.402850847135974No Hit
TCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG37980.20021297008929984No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGC37260.19641746354732262No Hit
TTCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT32260.17005977922803614No Hit
GAATGACTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCT30410.16030743602990014No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTC30130.15883140570802012No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACACAACTTCGTA29930.15777709833524864No Hit
TGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG29890.15756623686069435No Hit
GAACTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT28830.15197840778500565No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACACAACTT28590.15071323893767988No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTC27910.14712859387025692No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCG26880.14169891090048392No Hit
TTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG25250.13310630581239655No Hit
GCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG21360.1126000274119917No Hit
TTTCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTC20320.10711762907358012No Hit
TTGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT19220.1013189385233371No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA207.0344476E-445.00000410
CTCGTTA207.0344476E-445.00000433
CGATTAC406.8193913E-945.00000410
CGGTACC351.2124838E-745.0000046
ATCGACG207.0344476E-445.00000416
CGTTGAT253.8917693E-545.025
GCGATAG253.8917693E-545.09
ACGCTAG302.1662527E-644.9999961
CGTTTTT52100.042.754321
GTTCGCA603.6379788E-1241.24999641
TAGGGCG1450.040.3448265
TCGTTCA1850.040.13513616
CGTTGTA451.929402E-840.00000445
TTTCGCG4800.039.8437461
GCGATTA1300.039.8076939
TACGGGA3000.039.7500044
CTATGCG403.4598088E-739.3750041
TATTGCG800.039.3750041
GCGATAT403.4598088E-739.3750049
TTGGGAC56600.039.355125
Produced by FastQC (version 0.11.5)