##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549672_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1896980
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.257265232105766	33.0	31.0	34.0	30.0	34.0
2	32.393840209174584	34.0	31.0	34.0	30.0	34.0
3	32.422297019473056	34.0	31.0	34.0	30.0	34.0
4	35.944916129848494	37.0	35.0	37.0	35.0	37.0
5	35.969558456072285	37.0	35.0	37.0	35.0	37.0
6	35.75175594892935	37.0	35.0	37.0	35.0	37.0
7	35.937340404221445	37.0	35.0	37.0	35.0	37.0
8	35.86780988729454	37.0	35.0	37.0	35.0	37.0
9	37.767840989362035	39.0	38.0	39.0	35.0	39.0
10	37.32603295764847	39.0	37.0	39.0	34.0	39.0
11	37.19368364452973	39.0	37.0	39.0	34.0	39.0
12	37.133776845301476	39.0	37.0	39.0	34.0	39.0
13	37.11393109047012	39.0	37.0	39.0	33.0	39.0
14	38.15787883899672	40.0	38.0	41.0	33.0	41.0
15	38.206341131693534	40.0	38.0	41.0	33.0	41.0
16	38.32809887294542	40.0	38.0	41.0	34.0	41.0
17	38.32131914938481	40.0	38.0	41.0	34.0	41.0
18	38.33859186707293	40.0	38.0	41.0	34.0	41.0
19	38.33676264378117	40.0	37.0	41.0	34.0	41.0
20	38.24154076479457	40.0	37.0	41.0	34.0	41.0
21	38.203738573943845	40.0	37.0	41.0	34.0	41.0
22	38.119673375575914	40.0	37.0	41.0	34.0	41.0
23	37.867943257177195	40.0	37.0	41.0	33.0	41.0
24	37.497352634186974	39.0	36.0	41.0	32.0	41.0
25	37.42503241995171	39.0	36.0	41.0	32.0	41.0
26	37.58998618857342	39.0	36.0	41.0	33.0	41.0
27	37.71616938502251	39.0	36.0	41.0	33.0	41.0
28	37.7079415702854	39.0	36.0	41.0	33.0	41.0
29	37.71038334616074	40.0	36.0	41.0	33.0	41.0
30	37.58651013716539	39.0	36.0	41.0	33.0	41.0
31	37.493348374785185	39.0	36.0	41.0	33.0	41.0
32	37.38069088762138	39.0	36.0	41.0	33.0	41.0
33	37.24288764246328	39.0	36.0	41.0	32.0	41.0
34	37.12906725426731	39.0	35.0	41.0	32.0	41.0
35	37.12397336819576	39.0	35.0	41.0	32.0	41.0
36	37.045792786428954	39.0	35.0	41.0	32.0	41.0
37	37.04744541323578	39.0	35.0	41.0	32.0	41.0
38	36.93482324536895	39.0	35.0	41.0	31.0	41.0
39	36.854916235279234	39.0	35.0	41.0	31.0	41.0
40	36.759266834652976	39.0	35.0	41.0	31.0	41.0
41	36.75817246360004	39.0	35.0	41.0	31.0	41.0
42	36.83669147803351	39.0	35.0	41.0	31.0	41.0
43	36.72928180581767	39.0	35.0	41.0	31.0	41.0
44	36.65950563527291	39.0	35.0	41.0	31.0	41.0
45	36.6101951522947	39.0	35.0	40.0	31.0	41.0
46	36.46227424643381	39.0	35.0	40.0	31.0	41.0
47	36.36258843003089	39.0	35.0	40.0	30.0	41.0
48	36.36189416862592	39.0	35.0	40.0	30.0	41.0
49	36.37466921106179	39.0	35.0	40.0	31.0	41.0
50	36.22338770045019	39.0	35.0	40.0	30.0	41.0
51	35.474666048139675	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	8.0
11	6.0
12	12.0
13	10.0
14	16.0
15	32.0
16	59.0
17	108.0
18	273.0
19	527.0
20	1062.0
21	1805.0
22	3002.0
23	4310.0
24	6316.0
25	9202.0
26	12366.0
27	15032.0
28	17704.0
29	21714.0
30	27788.0
31	36314.0
32	48010.0
33	66111.0
34	103443.0
35	132333.0
36	178292.0
37	258589.0
38	436819.0
39	515415.0
40	299.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.181383040411603	3.9190186506974243	58.46919841010448	13.43039989878649
2	19.655926788896036	7.175932271294373	53.30319771426162	19.864943225547975
3	17.97609885185927	7.081888053643159	54.40848084850657	20.533532245990997
4	16.687998819175743	3.855391200750667	56.069963837257106	23.386646142816478
5	17.661915254773376	4.9704793935624	52.89507533026179	24.472530021402438
6	19.139158030132105	8.146000484981391	55.37955065419773	17.33529083068878
7	78.59766576347668	1.5144598256175605	14.607112357536717	5.280762053369039
8	79.55439698889815	4.07468713428713	10.055667429282334	6.315248447532394
9	74.57379624455713	4.754978966567913	12.472719796729539	8.19850499214541
10	37.28167930078335	31.922424063511478	17.366182036710985	13.429714598994192
11	23.647798079051967	25.63875212179359	29.86568124070892	20.84776855844553
12	21.498170776708243	23.285116342818586	33.92017838880747	21.2965344916657
13	22.840040485403115	23.62655378549062	36.23802043247689	17.29538529662938
14	19.53483958713323	27.023901148140727	32.19058714377589	21.250672120950142
15	15.58761821421417	27.15795633058862	35.67828864827251	21.57613680692469
16	17.518213159864626	28.034454764942176	31.0268953810794	23.420436694113803
17	17.98026336598172	28.101508713850436	30.52757540933484	23.390652510833007
18	18.111366487785848	26.419308585224936	33.4308216217356	22.038503305253617
19	18.26650781768917	28.522968086115824	31.60665900536642	21.60386509082858
20	20.126516884732577	30.692152790224466	31.099906166643827	18.08142415839914
21	20.53643159126612	30.5697477042457	30.475650771225844	18.418169933262345
22	18.51943615641704	28.06629484759987	29.381016141445876	24.033252854537213
23	18.29745173908001	29.733945534481123	28.017691277715105	23.95091144872376
24	19.37247625172643	29.082067285896528	27.65553669516811	23.88991976720893
25	17.266497274615443	32.085050975761476	25.580027201130218	25.068424548492867
26	17.16860483505361	31.258684856983205	28.021434068888446	23.55127623907474
27	17.9485287140613	30.254140792206556	28.861453468144106	22.935877025588038
28	14.893725816824638	30.38039410009594	30.002741199169208	24.723138883910213
29	16.912566289576063	28.61622157323746	28.125388775843707	26.345823361342767
30	18.886071545298318	32.3012894179169	26.494322554797627	22.318316481987157
31	17.875148920916402	35.36642452740672	23.244209216755053	23.51421733492182
32	16.72727176881148	34.823456230429414	22.780155826629695	25.669116174129407
33	16.87013041782201	32.08990078967622	24.813334879650814	26.226633912850954
34	15.766270598530296	33.279633944480175	25.295996794905584	25.658098662083944
35	14.468312791911353	31.721578508998515	24.301310504064354	29.508798195025776
36	15.509810330103639	34.56673238515957	25.157829813703884	24.765627471032907
37	14.834737319318075	33.31959219390821	26.956056468702887	24.88961401807083
38	14.87517000706386	33.74627038766882	25.13141941401596	26.247140191251354
39	16.374553237250787	32.87952429651341	27.22511571023416	23.520806756001644
40	16.91008866725005	32.96191841769549	26.9126190049447	23.215373910109754
41	15.708230977659227	31.8713428713007	25.687144830203795	26.733281320836277
42	16.850520300688462	28.036510664319074	28.80141066326477	26.311558371727696
43	17.633554386445823	27.874990774810488	29.93531824268047	24.556136596063215
44	17.690486984575482	28.25754620502061	26.755896213982222	27.29607059642168
45	18.591339919240056	28.20894263513585	24.92356271547407	28.276154730150026
46	19.446910352244092	30.037006188784275	26.835918143575576	23.680165315396053
47	13.95128045630423	31.0423409840905	30.438486436335648	24.56789212326962
48	14.858933673523179	29.586816940610866	28.899144956720686	26.655104429145272
49	17.97019473057175	26.90065261626375	30.5023774631256	24.626775190038906
50	17.66766122995498	26.28514797203977	27.72765131946568	28.319539478539575
51	15.881295532899662	25.92636717308564	25.651087518055014	32.541249775959685
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1146.0
1	2352.0
2	3558.0
3	7853.0
4	12148.0
5	7946.5
6	3745.0
7	4725.0
8	5705.0
9	7564.5
10	9424.0
11	11264.0
12	13104.0
13	13814.0
14	14524.0
15	14483.5
16	14443.0
17	13100.0
18	11757.0
19	11423.0
20	11089.0
21	11102.5
22	11116.0
23	11103.0
24	11090.0
25	12516.0
26	16205.5
27	18469.0
28	21348.5
29	24228.0
30	27553.0
31	30878.0
32	35215.5
33	39553.0
34	45461.5
35	51370.0
36	56938.0
37	62506.0
38	72455.0
39	82404.0
40	96903.0
41	111402.0
42	129383.5
43	147365.0
44	198482.5
45	249600.0
46	256760.5
47	263921.0
48	242417.0
49	220913.0
50	180427.0
51	139941.0
52	116048.5
53	92156.0
54	78189.0
55	64222.0
56	55978.5
57	47735.0
58	41854.0
59	35973.0
60	31249.5
61	26526.0
62	21973.5
63	17421.0
64	15117.0
65	12813.0
66	10375.0
67	7937.0
68	6135.0
69	4333.0
70	3991.5
71	3650.0
72	2931.5
73	2213.0
74	1796.5
75	1103.5
76	827.0
77	518.0
78	209.0
79	170.0
80	131.0
81	88.5
82	46.0
83	49.0
84	52.0
85	31.0
86	10.0
87	7.0
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1896980.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.302225986711612
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.88499730389303	19.5987930611927
2	13.491753200169414	7.906768024566076
3	5.822612961203378	5.118465624670123
4	3.1946436379627436	3.7444067930637934
5	1.9503095168848985	2.857420510389782
6	1.3603805404869875	2.3917306815164756
7	0.9366991550882566	1.9213159226770544
8	0.7067674568170044	1.656788779176424
9	0.5515404044868525	1.4545225415768475
>10	4.54107106546819	28.52802631551735
>50	0.43178164193685636	8.378615725176232
>100	0.11032942510583715	5.8584109981599175
>500	0.009831269008700225	2.0161730290733204
>1k	0.005643876653142721	3.4417326745869676
>5k	3.641210743963046E-4	0.8805586224402463
>10k+	0.001274423760387066	4.24627069621676
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCG	12819	0.6757583105778658	No Hit
TCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	12744	0.6718046579299729	No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	11611	0.6120781452624698	No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	11463	0.6042762707039611	No Hit
GAATCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTC	10538	0.5555145547132812	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCC	10235	0.5395417980157935	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGC	10186	0.5369587449525035	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8864	0.4672690276123101	No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	7642	0.402850847135974	No Hit
TCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG	3798	0.20021297008929984	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGC	3726	0.19641746354732262	No Hit
TTCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT	3226	0.17005977922803614	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCT	3041	0.16030743602990014	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTC	3013	0.15883140570802012	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACACAACTTCGTA	2993	0.15777709833524864	No Hit
TGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG	2989	0.15756623686069435	No Hit
GAACTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT	2883	0.15197840778500565	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACACAACTT	2859	0.15071323893767988	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTC	2791	0.14712859387025692	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCG	2688	0.14169891090048392	No Hit
TTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG	2525	0.13310630581239655	No Hit
GCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG	2136	0.1126000274119917	No Hit
TTTCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTC	2032	0.10711762907358012	No Hit
TTGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT	1922	0.1013189385233371	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0543073727714577E-4	0.0	0.0	0.641177028750962	0.0
2	1.0543073727714577E-4	0.0	0.0	2.4718236354626826	0.0
3	1.0543073727714577E-4	0.0	0.0	3.479794199200835	0.0
4	1.0543073727714577E-4	0.0	0.0	4.79277588588177	0.0
5	1.0543073727714577E-4	0.0	0.0	7.157323746164957	0.0
6	1.0543073727714577E-4	0.0	0.0	8.35807441301437	0.0
7	1.0543073727714577E-4	0.0	0.0	9.631414142479098	0.0
8	1.0543073727714577E-4	0.0	0.0	11.64524665520986	0.0
9	1.0543073727714577E-4	0.0	0.0	12.51731699859777	0.0
10	1.0543073727714577E-4	0.0	0.0	14.925934907062805	0.0
11	1.0543073727714577E-4	0.0	0.0	18.702200339486975	0.0
12	1.0543073727714577E-4	0.0	0.0	22.124165779291296	0.0
13	1.0543073727714577E-4	0.0	0.0	23.02048519225295	0.0
14	1.0543073727714577E-4	0.0	0.0	23.316956425476285	0.0
15	1.0543073727714577E-4	0.0	0.0	23.85048867146728	0.0
16	1.0543073727714577E-4	0.0	0.0	24.808695927210618	0.0
17	1.0543073727714577E-4	0.0	0.0	26.295849191873398	0.0
18	1.0543073727714577E-4	0.0	0.0	27.855169796202386	0.0
19	1.0543073727714577E-4	0.0	0.0	29.16920579025609	0.0
20	1.0543073727714577E-4	0.0	0.0	30.274647070606964	0.0
21	1.0543073727714577E-4	0.0	0.0	31.767335448976795	0.0
22	1.0543073727714577E-4	0.0	0.0	33.41943510210967	0.0
23	1.0543073727714577E-4	0.0	0.0	34.88987759491402	0.0
24	1.0543073727714577E-4	0.0	0.0	36.064481438918705	0.0
25	1.0543073727714577E-4	0.0	0.0	37.0464106105494	0.0
26	1.0543073727714577E-4	0.0	0.0	37.89597149152864	0.0
27	1.0543073727714577E-4	0.0	0.0	38.715748189227085	0.0
28	1.0543073727714577E-4	0.0	0.0	39.56984259190924	0.0
29	1.0543073727714577E-4	0.0	0.0	40.49025292833873	0.0
30	1.0543073727714577E-4	0.0	0.0	41.49332096279349	0.0
31	1.0543073727714577E-4	0.0	0.0	42.406403862982216	0.0
32	1.5814610591571867E-4	0.0	0.0	43.23582747314152	0.0
33	1.5814610591571867E-4	0.0	0.0	44.028297609885186	0.0
34	1.5814610591571867E-4	0.0	0.0	44.84464780862213	0.0
35	1.5814610591571867E-4	0.0	0.0	45.71782517475145	0.0
36	1.5814610591571867E-4	0.0	0.0	46.55056985313498	0.0
37	1.5814610591571867E-4	0.0	0.0	47.32422060327468	0.0
38	1.5814610591571867E-4	0.0	0.0	48.104882497443306	0.0
39	1.5814610591571867E-4	0.0	0.0	48.92529177956541	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	20	7.0344476E-4	45.000004	10
CTCGTTA	20	7.0344476E-4	45.000004	33
CGATTAC	40	6.8193913E-9	45.000004	10
CGGTACC	35	1.2124838E-7	45.000004	6
ATCGACG	20	7.0344476E-4	45.000004	16
CGTTGAT	25	3.8917693E-5	45.0	25
GCGATAG	25	3.8917693E-5	45.0	9
ACGCTAG	30	2.1662527E-6	44.999996	1
CGTTTTT	5210	0.0	42.75432	1
GTTCGCA	60	3.6379788E-12	41.249996	41
TAGGGCG	145	0.0	40.344826	5
TCGTTCA	185	0.0	40.135136	16
CGTTGTA	45	1.929402E-8	40.000004	45
TTTCGCG	480	0.0	39.843746	1
GCGATTA	130	0.0	39.807693	9
TACGGGA	300	0.0	39.750004	4
CTATGCG	40	3.4598088E-7	39.375004	1
TATTGCG	80	0.0	39.375004	1
GCGATAT	40	3.4598088E-7	39.375004	9
TTGGGAC	5660	0.0	39.35512	5
>>END_MODULE