FastQCFastQC Report
Sat 18 Jun 2016
SRR3549670_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549670_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169802
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT147611.261837473350191TruSeq Adapter, Index 27 (100% over 25bp)
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC124561.0647955807905953TruSeq Adapter, Index 27 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC108530.9277638437957876TruSeq Adapter, Index 27 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC96280.823045267489712TruSeq Adapter, Index 27 (96% over 25bp)
TCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG40440.3456995286381798TruSeq Adapter, Index 27 (100% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC34610.2958620347716964No Hit
TTCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT28570.24422936531139455TruSeq Adapter, Index 27 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG26240.22431146467521854TruSeq Adapter, Index 27 (100% over 23bp)
TTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG26020.22243080452931352TruSeq Adapter, Index 27 (100% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24010.20524840955990845No Hit
GCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG22960.19627253159081623TruSeq Adapter, Index 27 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG18140.15506897748507867TruSeq Adapter, Index 27 (100% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC18100.15472703927673231TruSeq Adapter, Index 27 (100% over 21bp)
TTTCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC15700.13421074677595013TruSeq Adapter, Index 27 (100% over 21bp)
GTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG15680.13403977767177694TruSeq Adapter, Index 27 (95% over 24bp)
TTGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT14840.12685907529650317TruSeq Adapter, Index 27 (95% over 23bp)
GTTCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC14320.12241387858800037TruSeq Adapter, Index 27 (100% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT14010.119763857473316TruSeq Adapter, Index 27 (100% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC13070.1117283095771763No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTC12050.10300888526434389No Hit
GTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC11770.10061531780591929TruSeq Adapter, Index 27 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGT302.1655342E-645.00000420
CGAATAT2050.045.00000414
TCGTAAA302.1655342E-645.00000437
TTAGTCG302.1655342E-645.0000041
AACCGTA207.033339E-445.038
ATTCGAT207.033339E-445.012
GCGTTAG551.8189894E-1245.01
ACGGGTA253.890848E-545.05
AGTGCCG207.033339E-445.011
GTCGTAG351.2119563E-745.01
AATTCCG207.033339E-445.011
GCGATAT253.890848E-545.09
CCGACAC207.033339E-445.016
TTAAACG207.033339E-445.043
TTCGGAC207.033339E-445.013
CCGGTCC207.033339E-445.011
TTGCACG351.2119563E-745.01
CGCAATC253.890848E-545.018
CGCGTCA207.033339E-445.017
CGATCAA207.033339E-445.011
Produced by FastQC (version 0.11.5)